Detection of the European mountain ash ringspot-associated virus (EMARaV) in Sorbus aucuparia L. in several European countries: a study about virus diseases in mountain ash trees
Saved in:
| Main Author: | |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
Uelvesbüll
Der Andere Verlag
[2014]
|
| Series: | Berliner ökophysiologische und phytomedizinische Schriften
30 |
| Subjects: | |
| Online Access: | Inhaltsverzeichnis Inhaltsverzeichnis |
| Physical Description: | VI, 40 Seiten, vii Illustrationen |
| ISBN: | 9783862474417 |
Staff View
MARC
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Record in the Search Index
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|---|---|
| adam_text | CONTENTS
ABBREVIATIONS II
LIST OF FIGURES IV
LIST OF TABLES VI
1 INTRODUCTION 1
2 MATERIAL AND METHODS 6
2.1 MATERIAL 6
2.1.1 PLANT MATERIAL 6
2.1.2 BACTERIA 8
2.1.3 CHEMICALS AND KITS 8
2.1.4 LABORATORY EQUIPMENT 8
2.2 METHODS 10
2.2.1 RNA ISOLATION ACCORDING TO BOOM ET AL. (1990) MODIFIED BY MIELKE
ET AL. (2007)
EXCEPT PHENOL/CHLOROFORM EXTRACTION 10
2.2.2 PHOTOMETRIC DETERMINATION OF THE RNA CONCENTRATION WITH UV
SPECTROSCOPY ...11
2.2.3 CDNA-SYNTHESIS 12
2.2.4 POLYMERASE CHAIN REACTION 13
2.2.5 GEL ELECTROPHORESIS 14
2.2.6 PURIFICATION OF PCR PRODUCTS 15
2.2.7 CLONING OF PCR FRAGMENT 16
2.2.8 COLONY PCR 17
2.2.9 PLASMID ISOLATION FROM E.COLI 17
2.2.10 SEQUENCING AND ANALYSES 18
3 RESULTS 19
3.1 DETECTION OF EMARAV IN EUROPEAN MOUNTAIN ASH TREES 19
3.1.1 RNA EXTRACTION 19
3.1.2 RT-PCR 20
3.2 COMPARISON OF RT-PCR USING VARIOUS ENZYMES 23
3.3 CLONING OF PCR PRODUCTS AND COMPARISON OF VARIOUS EMARAV ISOLATES
FROM
EUROPEAN ROWAN TREES BY SEQUENCING 25
3.3.1 PURIFICATION AND COLONY PCR 25
3.3.2 SEQUENCE ANALYSIS 26
3.4 SEQUENCE DIVERSITY OF EMARAV 26
4 DISCUSSION 30
5 SUMMARY 36
6 BIBLIOGRAPHY 35
APPENDIX
I
HTTP://D-NB.INFO/106434013X
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| author_facet | Dieckmann, Heike Luisa |
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| discipline | Biologie |
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| id | DE-604.BV043298896 |
| illustrated | Illustrated |
| indexdate | 2024-07-10T07:22:33Z |
| institution | BVB |
| isbn | 9783862474417 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-028719828 |
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| physical | VI, 40 Seiten, vii Illustrationen |
| publishDate | 2014 |
| publishDateSearch | 2014 |
| publishDateSort | 2014 |
| publisher | Der Andere Verlag |
| record_format | marc |
| series | Berliner ökophysiologische und phytomedizinische Schriften |
| series2 | Berliner ökophysiologische und phytomedizinische Schriften |
| spelling | Dieckmann, Heike Luisa aut Detection of the European mountain ash ringspot-associated virus (EMARaV) in Sorbus aucuparia L. in several European countries a study about virus diseases in mountain ash trees submitted by: Heike Luisa Dieckmann Uelvesbüll Der Andere Verlag [2014] 2014 VI, 40 Seiten, vii Illustrationen txt rdacontent n rdamedia nc rdacarrier Berliner ökophysiologische und phytomedizinische Schriften 30 European mountain ash ringspot-associated virus (DE-588)1075258138 gnd rswk-swf European mountain ash ringspot-associated virus (DE-588)1075258138 s DE-604 Berliner ökophysiologische und phytomedizinische Schriften 30 (DE-604)BV035817225 30 application/pdf http://d-nb.info/106434013X/04 Inhaltsverzeichnis DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=028719828&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Dieckmann, Heike Luisa Detection of the European mountain ash ringspot-associated virus (EMARaV) in Sorbus aucuparia L. in several European countries a study about virus diseases in mountain ash trees Berliner ökophysiologische und phytomedizinische Schriften European mountain ash ringspot-associated virus (DE-588)1075258138 gnd |
| subject_GND | (DE-588)1075258138 |
| title | Detection of the European mountain ash ringspot-associated virus (EMARaV) in Sorbus aucuparia L. in several European countries a study about virus diseases in mountain ash trees |
| title_auth | Detection of the European mountain ash ringspot-associated virus (EMARaV) in Sorbus aucuparia L. in several European countries a study about virus diseases in mountain ash trees |
| title_exact_search | Detection of the European mountain ash ringspot-associated virus (EMARaV) in Sorbus aucuparia L. in several European countries a study about virus diseases in mountain ash trees |
| title_full | Detection of the European mountain ash ringspot-associated virus (EMARaV) in Sorbus aucuparia L. in several European countries a study about virus diseases in mountain ash trees submitted by: Heike Luisa Dieckmann |
| title_fullStr | Detection of the European mountain ash ringspot-associated virus (EMARaV) in Sorbus aucuparia L. in several European countries a study about virus diseases in mountain ash trees submitted by: Heike Luisa Dieckmann |
| title_full_unstemmed | Detection of the European mountain ash ringspot-associated virus (EMARaV) in Sorbus aucuparia L. in several European countries a study about virus diseases in mountain ash trees submitted by: Heike Luisa Dieckmann |
| title_short | Detection of the European mountain ash ringspot-associated virus (EMARaV) in Sorbus aucuparia L. in several European countries |
| title_sort | detection of the european mountain ash ringspot associated virus emarav in sorbus aucuparia l in several european countries a study about virus diseases in mountain ash trees |
| title_sub | a study about virus diseases in mountain ash trees |
| topic | European mountain ash ringspot-associated virus (DE-588)1075258138 gnd |
| topic_facet | European mountain ash ringspot-associated virus |
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| volume_link | (DE-604)BV035817225 |
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