Arabidopsis a practical approach (Oxford University Press, 2000)
Includes bibliographical references and index
1 - Growth, maintenance, and use of Arabidopsis genetic resources - Mary Anderson, Fiona Wilson - 1 -- - 1 - What is Arabidopsis? - 1 -- - 2 - What makes Arabidopsis such an attractive experimental model? - 3 -- - 3 - Arabidopsis genetic resource centres - 4 -- - Arabidopsis genetic resources - 4 -- - Accessing Arabidopsis resources - 5 -- - 4 - Mutants of Arabidopsis - 5 -- - Single gene mutation lines - 5 -- - Resources for the identification/investigation of novel genes - 8 -- - Mapping tools - 10 -- - 5 - Considerations of available resources for identifying novel genes - 11 -- - Forward genetics - 12 -- - Reverse genetics - 14 -- - 6 - Growing Arabidopsis - 15 -- - How to maintain clean growth conditions - 15 -- - Growing Arabidopsis in the glasshouse - 16 -- - Chemical control of pests and diseases - 19 -- - 7 - Seed storage - 21 -- - 8 - Growing Arabidopsis with specific growth requirements - 23 -- - 9 - Sterile culture of Arabidopsis - 24 -- - 2
- Preservation and handling of stock centre clones - Randy Scholl, Keith Davis, Doreen Ware - 29 -- - 2 - Missions of a plant DNA resource centre - 29 -- - 3 - Preservation of stocks - 30 -- - Plasmids with small DNA inserts - 30 -- - Cosmids - 32 -- - Phage and phage libraries - 33 -- - Yeast artificial chromosome (YAC) libraries - 33 -- - Pools of YAC library cells for PCR screening - 37 -- - Distribution of YAC libraries arrayed on nylon filters - 38 -- - Other large-insert libraries - 38 -- - Yeast expression analysis--'two-hybrid' libraries and complementation testing - 42 -- - 4 - Verification of stock identity and purity - 42 -- - 5 - Pooled DNA from T-DNA lines for PCR screening - 44 -- - 6 - Organization of stock information - 48 -- - Collecting, maintaining, and disseminating stock data - 48 -- - Organizing and distributing patron data - 48 -- - 7 - Future - 48 -- - 3 - Genetic mapping using recombinant inbred lines - Clare Lister, Mary Anderson, Caroline Dean
- 51 -- - 2 - Preparation and digestion of A. thaliana genomic DNA - 54 -- - Preparation of genomic DNA - 56 -- - Identifying an RFLP - 60 -- - Southern blotting and hybridization - 61 -- - 3 - Polymorphic markers - 62 -- - RFLP markers - 62 -- - PCR-based markers - 66 -- - Phenotypic and biochemical markers - 68 -- - 4 - Calculating map positions - 69 -- - Mapping programs - 69 -- - NASC mapping service - 70 -- - 5 - Integration of a mutation into a molecular map - 72 -- - 4 - Arabidopsis mutant characterization; microscopy, mapping, and gene expression analysis - Kriton Kalantidis, L. Greg Briarty, Zoe A. Wilson - 77 -- - 1 - Generation of mutants and their importance for developmental biology - 77 -- - 2 - Mapping and segregation analysis - 78 -- - Mapping of mutations - 78 -- - Influence of environment on phenotype - 82 -- - 3 - Microscopy - 82 -- - Fresh material characterization - 82 -- - Fixed material characterization - 84 -- - 4 - Analysis of plant gene expression
- 89 -- - RNA isolation - 89 -- - Northern analysis - 91 -- - In situ hybridizations - 92 -- - 5 - Classical and molecular cytogenetics of Arabidopsis - G.H. Jones, J.S. Heslop-Harrison - 105 -- - 2 - Mitotic chromosome analysis by light microscopy - 108 -- - 3 - In situ hybridization to mitotic chromosome preparations - 112 -- - Photography of in situ hybridizations - 117 -- - 4 - Meiotic chromosome analysis by light microscopy - 117 -- - 5 - Meiotic chromosome analysis by electron microscopy - 121 -- - 6 - Tissue culture, transformation, and transient gene expression in Arabidopsis - Keith Lindsey, Wenbin Wei - 125 -- - 2 - Stable transformation by Agrobacterium tumefaciens - 128 -- - 3 - Transient gene expression in Arabidopsis protoplasts - 131 -- - Reporter gene enzyme assays - 134 -- - 7 - Transposon and T-DNA mutagenesis - Mark G.M. Aarts, Csaba Koncz, Andy Pereira - 143 -- - 2 - Transposon tagging - 143 -- - Endogenous transposable elements - 143 --