Human stem cell technology and biology a research guide and laboratory manual (Wiley-Blackwell, 2011)
Includes bibliographical references and index
Frontmatter -- Section I: Introduction. Introduction to Pluripotent Stem Cells: Biology and Applications / Maria Borowski, Gary S Stein -- Researching and Obtaining Established Stem Cell Lines / Mai X Luong, Kelly P Smith, Gary S Stein -- Section II: Laboratory Guide for Human Stem Cell Culture: Pluripotent Stem Cell Culture. Basics of Cell Culture / Alicia Allaire, Mai X Luong, Kelly P Smith -- The Stem Cell Laboratory / Alicia Allaire, Mai X Luong, Kelly P Smith -- Reagent Preparation / Alicia Allaire, Mai X Luong, Kelly P Smith -- Preparation of Mouse Embryonic Fibroblasts for Culture of Human Embryonic Stem Cells / Meng-Jiao Shi, Maria Borowski, Kimberly Stencel -- Thawing and Seeding of Frozen Mouse Embryonic Fibroblasts / Meng-Jiao Shi, Maria Borowski, Kimberly Stencel -- Thawing, Seeding, and Changing the Medium of Human Embryonic Stem Cells / Meng-Jiao Shi, Kimberly Stencel, Maria Borowski --
- Passaging of Human Embryonic Stem Cells on Inactivated Mouse Embryonic Fibroblast Plates / Meng-Jiao Shi, Kimberly Stencel, Maria Borowski -- Harvesting Human Embryonic Stem Cells for Cryopreservation / Meng-Jiao Shi, Kimberly Stencel, Maria Borowski -- Human Embryonic Stem Cell Culture on BD Matrigel₉ with mTeSRʼ1 Medium / Meng-Jiao Shi, Kimberly Stencel, Maria Borowski -- Section III: Laboratory Guide for Human Stem Cell Culture: Characterization of Pluripotent Stem Cells. Defining Pluripotency / Kelly P Smith, Mai X Luong -- Characterization of Human Embryonic Stem Cells by Immunofluorescence Microscopy / Shirwin M Pockwinse, Prachi N Ghule -- Preparation of Human Embryonic Stem Cell Samples for Flow Cytometry / Meng-Jiao Shi, Kimberly Stencel, Maria Borowski -- Characterization of Human Embryonic Stem Cell Gene Expression Using Reverse Transcription Polymerase Chain Reaction / Alicia Allaire, Kelly P Smith, Mai X Luong --
- Determining Pluripotency of Human Embryonic Stem Cells: Embryoid Body Formation / Shirwin M Pockwinse, Prachi N Ghule -- Characterization of Human Embryonic Stem Cells by Cytogenetics: Karyotyping and Fluorescence Hybridization / Shirwin M Pockwinse, Prachi N Ghule, Anne Higgins -- High-Resolution Chromatin Immunoprecipitation Assay / Beatriz P̌rez-Cadah̕a, Bojan Drobic, James R Davie -- Assaying Pluripotency Via Teratoma Formation / M William Lensch, Tan A Ince -- Section IV: Perspectives in Human Stem Cell Technologies. Genomic Analysis of Pluripotent Stem Cells / David Lapointe -- Proteomic Analysis of Human Pluripotent Cells / Andy T Y Lau, Yan-Ming Xu, Jen-Fu Chiu -- Bioinformatics Strategies for Understanding Gene Expression in Human Pluripotent Cells / Gustavo Glusman, Bruz Marzolf, Kai Wang, Ji-Hoon Cho, Burak Kutlu, Qiang Tian -- Epigenetic Analysis of Pluripotent Cells / Mojgan Rastegar, Genevïve P Delcuve, James R Davie --
- Differentiation of Pluripotent Stem Cells: An Overview / Jeremy Micah Crook -- Cellular Reprogramming: Current Technology, Perspectives, and Generation of Induced Pluripotent Cells / Tanja Dominko -- Section V: Applications of Human Embryonic Stem Cells. Human Pluripotent Cells: The Biology of Pluripotency / Li-Fang Chu, Thomas P Zwaka -- Human Pluripotent Cells for Regenerative Medicine: Potential Applications for Regenerative Medicine / Christopher C Ford, Darrell N Kotton -- Therapeutic Applications of Human Embryonic Stem Cells / Shi-Jiang Lu, Irina Klimanskaya, Edmund Mickunas, Robert Lanza -- Human Stem Cells for Drug Screening, Toxicity, Specificity, and Off-Target Effects / Arnaud Lacoste, Mark Burcin -- Interfacing Stem Cells with Gene Therapy / Christian Mueller, Terence R Flotte -- Tissue Engineering for Stem Cell Mediated Regenerative Medicine / Janet Zoldan, Thomas P Kraehenbuehl, Abigail K R Lytton-Jean, Robert S Langer, Daniel G Anderson -- Glossary -- Index