Virology methods manual (Academic Press, 1996)
The Virology Methods Manual is a comprehensive source of methods for the study, manipulation, and detection of viruses. Edited by Brian Mahy and Hillar Kangro, this work describes the most up-to-date, definitive techniques, provided by experts in each area, and presented with easy-to-use, step-by-step protocols. This new manual will satisfy the needs of virologists and all those working with viruses who need a practical guide to methods that work! Key Features * Provides up-to-date techniques by experts worldwide * Presents common, step-by-step protocols in an attractive, easy-to-use fashion * Contains useful appendices including virus taxonomy, metabolic inhibitors, and Bio-safety in the virology laboratory
Classical Techniques: -- V.C. George, J.C. Hierholzer, and E.W. Ades, Cell Culture. -- J.C. Hierholzer and R.A. Killington, Virus Isolation and Quantitation. -- J.C. Hierholzer, R.A. Killington, and A. Stokes, Preparation of Antigens. -- R.A. Killington, A. Stokes, and J.C. Hierholzer, Virus Purification. -- I.L. Chrystie, Electron Microscopy. -- M. Chernesky, Traditional and Serological Tests. -- M. Chernesky and J. Mahoney, Immunoassays. -- A. Mawle, Cell Mediated Immunity. -- Techniques in Molecular Virology: -- J.E. Novak, T.C. Jarvis, and K. Kirkegaard, RNA: Transcription, Transfection, and Quantitation. -- D.J. McCance, DNA Viruses: DNA Extraction, Purification, and Characterization. -- K.N. Leppard and C.R. Pringle, Virus Mutants. -- D.R. Harper and B.F. Coles, Polypeptides. -- M. McCrae, The Assay of Viral Enzyme. -- Medical Virology: -- A.L. Jabri, D. Wigg, and J.S. Oxford, Initial In vitro Screening of DrugCandidates for their Potential Antiviral Activities. -- S. Specter and D. Jeffries, Detection of Virus and Viral Antigens. -- J. Mahoney, Detection of Viral Nucleic Acids in Clinical Material. -- S. Specter and D. Jeffries, Serological Methods. -- S. Specter and D. Jeffries, Disinfection. Appendices. -- Subject Index
Includes bibliographical references and index
Cell culture / V.G. George, J.C. Hierholzer and E.W. Ades -- Virus isolation and quantitation / J.C. Hierholzer and R.A. Killington -- Preparation of antigens / J.C. Hierholzer, R.A. Killington and A. Stokes -- Virus purification / R.A. Killington, A. Stokes and J.C. Hierholzer -- Electron microscopy / I.L. Chrystie -- Traditional serological tests / M.A. Chernesky -- Immunoassays : principles and assay design / M.A. Chernesky and J.B. Mahony -- Cell mediated immunity / A.C. Mawle -- RNA : transcription, transfection and quantitation / J.E. Novak, T.C. Jarvis and K. Kirkegaard -- DNA viruses : DNA extraction, purification and characterization / D.J. McCance -- Virus mutants / K.N. Leppard and C.R. Pringle -- Polypeptides / D.R. Harper and B.F. Coles -- The assay of viral enzymes / M. McCrae -- Initial in vitro screening of drug candidates for their potential antiviral activities / A.A. Al-Jabri, M.D. Wigg and J.S. Oxford -- Detection of virus and viral antigens / S. Specter and D. Jeffries -- Detection of viral nucleic acids in clinical material / J.B. Mahony -- Serological diagnosis ; Disinfection / S. Specter and D. Jeffries