Essentials of nucleic acid analysis: a robust approach
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| Format: | Electronic eBook |
|---|---|
| Language: | English |
| Published: |
Cambridge, UK
Royal Society of Chemistry
©2008
|
| Subjects: | |
| Online Access: | FAW01 FAW02 Volltext |
| Item Description: | Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002 Includes bibliographical references and index Valid analytical molecular biology : the challenge -- Quality in the analytical molecular biology laboratory -- An introduction to method validation -- DNA extraction -- DNA quantification -- PCR : factors affecting reliability and validity -- Quantitative real-time PCR analysis -- Multiplex PCR and whole genome amplification -- Procedures for quality control of RNA samples for use in quantitative reverse transcription PCR -- Microarrays Over the last decade there has been a rapid development of molecular techniques, with an increasing range of instrumentation now available. The development of accompanying reference literature has not kept pace with technological advances and this poses significant challenges to the analyst. Essentials of Nucleic Acid Analysis sets out to guide the analyst through the steps needed to obtain good quality results in DNA analysis. The underlying principles for achieving this goal were formulated by LGC (formerly the Laboratory of the Government Chemist) as the six principles for ensuring valid analytical measurement, which are detailed in the introduction. The reader is also provided with guidelines for method validation and quality control of established and emerging DNA measurement techniques. The authors of each chapter are practitioners of the art of DNA analysis in areas where the quality of the result is critical. Technical details and examples of application of key techniques in nucleic acid analysis are provided while highlighting best practice, available standards and practical advice on improving measurement quality. This book provides an indispensable handbook and premier reference for those working in the widely varying areas and specifically in the fields of food analysis and forensic applications |
| Physical Description: | 1 Online-Ressource (xxii, 248 pages) |
| ISBN: | 1615833595 1847558216 9781615833597 9781847558213 |
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| spelling | Essentials of nucleic acid analysis a robust approach edited by Jacquie T. Keer and Lyndsey Birch Cambridge, UK Royal Society of Chemistry ©2008 1 Online-Ressource (xxii, 248 pages) txt rdacontent c rdamedia cr rdacarrier Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002 Includes bibliographical references and index Valid analytical molecular biology : the challenge -- Quality in the analytical molecular biology laboratory -- An introduction to method validation -- DNA extraction -- DNA quantification -- PCR : factors affecting reliability and validity -- Quantitative real-time PCR analysis -- Multiplex PCR and whole genome amplification -- Procedures for quality control of RNA samples for use in quantitative reverse transcription PCR -- Microarrays Over the last decade there has been a rapid development of molecular techniques, with an increasing range of instrumentation now available. The development of accompanying reference literature has not kept pace with technological advances and this poses significant challenges to the analyst. Essentials of Nucleic Acid Analysis sets out to guide the analyst through the steps needed to obtain good quality results in DNA analysis. The underlying principles for achieving this goal were formulated by LGC (formerly the Laboratory of the Government Chemist) as the six principles for ensuring valid analytical measurement, which are detailed in the introduction. The reader is also provided with guidelines for method validation and quality control of established and emerging DNA measurement techniques. The authors of each chapter are practitioners of the art of DNA analysis in areas where the quality of the result is critical. Technical details and examples of application of key techniques in nucleic acid analysis are provided while highlighting best practice, available standards and practical advice on improving measurement quality. This book provides an indispensable handbook and premier reference for those working in the widely varying areas and specifically in the fields of food analysis and forensic applications Analytical chemistry Molecular biology Science Analytical chemistry bicssc Molecular biology bicssc SCIENCE / Chemistry / Organic bisacsh Science eflch Nucleic acids / Analysis fast Nucleic Acids / analysis Chemie Naturwissenschaft Nucleic acids Analysis Biochemische Analyse (DE-588)4255721-5 gnd rswk-swf Chemische Analyse (DE-588)4009840-0 gnd rswk-swf Nucleinsäuren (DE-588)4172117-2 gnd rswk-swf 1\p (DE-588)4143413-4 Aufsatzsammlung gnd-content Nucleinsäuren (DE-588)4172117-2 s Biochemische Analyse (DE-588)4255721-5 s 2\p DE-604 Chemische Analyse (DE-588)4009840-0 s 3\p DE-604 Keer, Jacquie T. Sonstige oth Birch, Lyndsey Sonstige oth Erscheint auch als Druck-Ausgabe, Hardcover 0-85404-367-5 Erscheint auch als Druck-Ausgabe, Hardcover 978-0-85404-367-5 http://search.ebscohost.com/login.aspx?direct=true&scope=site&db=nlebk&db=nlabk&AN=496360 Aggregator Volltext 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk 2\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk 3\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Essentials of nucleic acid analysis a robust approach Analytical chemistry Molecular biology Science Analytical chemistry bicssc Molecular biology bicssc SCIENCE / Chemistry / Organic bisacsh Science eflch Nucleic acids / Analysis fast Nucleic Acids / analysis Chemie Naturwissenschaft Nucleic acids Analysis Biochemische Analyse (DE-588)4255721-5 gnd Chemische Analyse (DE-588)4009840-0 gnd Nucleinsäuren (DE-588)4172117-2 gnd |
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| title | Essentials of nucleic acid analysis a robust approach |
| title_auth | Essentials of nucleic acid analysis a robust approach |
| title_exact_search | Essentials of nucleic acid analysis a robust approach |
| title_full | Essentials of nucleic acid analysis a robust approach edited by Jacquie T. Keer and Lyndsey Birch |
| title_fullStr | Essentials of nucleic acid analysis a robust approach edited by Jacquie T. Keer and Lyndsey Birch |
| title_full_unstemmed | Essentials of nucleic acid analysis a robust approach edited by Jacquie T. Keer and Lyndsey Birch |
| title_short | Essentials of nucleic acid analysis |
| title_sort | essentials of nucleic acid analysis a robust approach |
| title_sub | a robust approach |
| topic | Analytical chemistry Molecular biology Science Analytical chemistry bicssc Molecular biology bicssc SCIENCE / Chemistry / Organic bisacsh Science eflch Nucleic acids / Analysis fast Nucleic Acids / analysis Chemie Naturwissenschaft Nucleic acids Analysis Biochemische Analyse (DE-588)4255721-5 gnd Chemische Analyse (DE-588)4009840-0 gnd Nucleinsäuren (DE-588)4172117-2 gnd |
| topic_facet | Analytical chemistry Molecular biology Science SCIENCE / Chemistry / Organic Nucleic acids / Analysis Nucleic Acids / analysis Chemie Naturwissenschaft Nucleic acids Analysis Biochemische Analyse Chemische Analyse Nucleinsäuren Aufsatzsammlung |
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