Bioconjugate techniques:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
San Diego
Academic Press
©1996
|
| Schlagworte: | |
| Online-Zugang: | FAW01 FAW02 Volltext |
| Beschreibung: | Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002 Includes bibliographical references (pages 673-725) and index Functional targets -- The chemistry of reactive groups -- Zero-length cross-linkers -- Homobifunctional cross-linkers -- Heterobifunctional cross-linkers -- Trifunctional cross-linkers -- Cleavable reagent systems -- Tags and probes -- Preparation of hapten-carrier immunogen conjugates -- Antibody modification and conjugation -- Immunotoxin conjugation techniques -- Preparation of liposome conjugates and derivatives -- Avidin-biotin systems -- Preparation of colloidal-gold-labeled proteins -- Modification with synthetic polymers -- Enzyme modification and conjugation -- Nucleic acid and oligonucleotide modification and conjugation Bioconjugate Techniques is the essential guide to the modification and crosslinking of biomolecules for use in research, diagnostics, and therapeutics. It provides highly detailed information on the chemistry, reagent systems, and practical applications for creating labeled or conjugate molecules. It also describes dozens of reactions with details on hundreds of commercially available reagents and the use of these reagents for modifying or crosslinking peptides and proteins, sugars and polysaccharides, nucleic acids and oligonucleotides, lipids, and synthetic polymers. Armed with this information and the abundant protocols provided, readers will form unique complexes that can be used for detecting, quantifying, and targeting important analytes. This book helps readers make: high activity antibody-enzymes conjugates, immunotoxins, immunogen complexes, liposome conjugates; as well as biotinylated molecules, avidin or streptavidin conjugates, colloidal gold labeled proteins, PEG or dextran complexes, labeled oligonucleotide probes, and fluorescently tagged or radiolabeled molecules. Key Features * This book is the first to thoroughly capture the entire field of bioconjugate chemistry in a single volume * Serves as a practical guide to modification and cross-linking technology for research, diagnostics, and therapeutics * Provides useful, detailed, easy-to-follow, step-by-step protocols * Contains easy-to-read, and easy-to-understand key concepts for making bioconjugates of all types * Efficiently covers the chemistry of bioconjugation, the major reagents available for modification and cross-linking, and the application of these reagents to the synthesis of highly active conjugates * Cites over more than references keyed to concepts covered in the book * Uses more than 600 figures to illustrate bioconjugate reagents, their reactions, and applications * Suggests sources for all key reagents |
| Beschreibung: | 1 Online-Ressource (xxv, 785 pages) |
| ISBN: | 0080527906 012342335X 0123423368 1281019607 9780080527901 9780123423351 9780123423368 9781281019608 |
Internformat
MARC
| LEADER | 00000nmm a2200000zc 4500 | ||
|---|---|---|---|
| 001 | BV043044836 | ||
| 003 | DE-604 | ||
| 005 | 20161017 | ||
| 007 | cr|uuu---uuuuu | ||
| 008 | 151123s1996 |||| o||u| ||||||eng d | ||
| 020 | |a 0080527906 |c electronic bk. |9 0-08-052790-6 | ||
| 020 | |a 012342335X |9 0-12-342335-X | ||
| 020 | |a 0123423368 |c paper : alk. paper |9 0-12-342336-8 | ||
| 020 | |a 1281019607 |9 1-281-01960-7 | ||
| 020 | |a 9780080527901 |c electronic bk. |9 978-0-08-052790-1 | ||
| 020 | |a 9780123423351 |9 978-0-12-342335-1 | ||
| 020 | |a 9780123423368 |c paper : alk. paper |9 978-0-12-342336-8 | ||
| 020 | |a 9781281019608 |9 978-1-281-01960-8 | ||
| 035 | |a (OCoLC)162128986 | ||
| 035 | |a (DE-599)BVBBV043044836 | ||
| 040 | |a DE-604 |b ger |e aacr | ||
| 041 | 0 | |a eng | |
| 049 | |a DE-1046 |a DE-1047 | ||
| 082 | 0 | |a 574.19/296 |2 22 | |
| 100 | 1 | |a Hermanson, Greg T. |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a Bioconjugate techniques |c Greg T. Hermanson |
| 264 | 1 | |a San Diego |b Academic Press |c ©1996 | |
| 300 | |a 1 Online-Ressource (xxv, 785 pages) | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b c |2 rdamedia | ||
| 338 | |b cr |2 rdacarrier | ||
| 500 | |a Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002 | ||
| 500 | |a Includes bibliographical references (pages 673-725) and index | ||
| 500 | |a Functional targets -- The chemistry of reactive groups -- Zero-length cross-linkers -- Homobifunctional cross-linkers -- Heterobifunctional cross-linkers -- Trifunctional cross-linkers -- Cleavable reagent systems -- Tags and probes -- Preparation of hapten-carrier immunogen conjugates -- Antibody modification and conjugation -- Immunotoxin conjugation techniques -- Preparation of liposome conjugates and derivatives -- Avidin-biotin systems -- Preparation of colloidal-gold-labeled proteins -- Modification with synthetic polymers -- Enzyme modification and conjugation -- Nucleic acid and oligonucleotide modification and conjugation | ||
| 500 | |a Bioconjugate Techniques is the essential guide to the modification and crosslinking of biomolecules for use in research, diagnostics, and therapeutics. It provides highly detailed information on the chemistry, reagent systems, and practical applications for creating labeled or conjugate molecules. It also describes dozens of reactions with details on hundreds of commercially available reagents and the use of these reagents for modifying or crosslinking peptides and proteins, sugars and polysaccharides, nucleic acids and oligonucleotides, lipids, and synthetic polymers. Armed with this information and the abundant protocols provided, readers will form unique complexes that can be used for detecting, quantifying, and targeting important analytes. This book helps readers make: high activity antibody-enzymes conjugates, immunotoxins, immunogen complexes, liposome conjugates; as well as biotinylated molecules, avidin or streptavidin conjugates, colloidal gold labeled proteins, PEG or dextran complexes, labeled oligonucleotide probes, and fluorescently tagged or radiolabeled molecules. Key Features * This book is the first to thoroughly capture the entire field of bioconjugate chemistry in a single volume * Serves as a practical guide to modification and cross-linking technology for research, diagnostics, and therapeutics * Provides useful, detailed, easy-to-follow, step-by-step protocols * Contains easy-to-read, and easy-to-understand key concepts for making bioconjugates of all types * Efficiently covers the chemistry of bioconjugation, the major reagents available for modification and cross-linking, and the application of these reagents to the synthesis of highly active conjugates * Cites over more than references keyed to concepts covered in the book * Uses more than 600 figures to illustrate bioconjugate reagents, their reactions, and applications * Suggests sources for all key reagents | ||
| 650 | 4 | |a Bioconjugates / Synthesis. Biochemistry / methods. Proteins / chemistry. Peptides / chemistry. Nucleic Acids / chemistry | |
| 650 | 7 | |a SCIENCE / Life Sciences / Biochemistry |2 bisacsh | |
| 650 | 7 | |a Biochemie |2 gtt | |
| 650 | 7 | |a Wetenschappelijke technieken |2 gtt | |
| 650 | 4 | |a Biochemistry / methods | |
| 650 | 4 | |a Proteins / chemistry | |
| 650 | 4 | |a Peptides / chemistry | |
| 650 | 4 | |a Nucleic Acids / chemistry | |
| 650 | 4 | |a Wissenschaftliches Arbeiten | |
| 650 | 4 | |a Bioconjugates |x Synthesis | |
| 650 | 0 | 7 | |a Proteindesign |0 (DE-588)4346801-9 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Nucleinsäuren |0 (DE-588)4172117-2 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Biomolekül |0 (DE-588)4135124-1 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Vernetzung |g Chemie |0 (DE-588)4139250-4 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Biotechnologie |0 (DE-588)4069491-4 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Konjugate |0 (DE-588)4131219-3 |2 gnd |9 rswk-swf |
| 689 | 0 | 0 | |a Konjugate |0 (DE-588)4131219-3 |D s |
| 689 | 0 | 1 | |a Biomolekül |0 (DE-588)4135124-1 |D s |
| 689 | 0 | 2 | |a Vernetzung |g Chemie |0 (DE-588)4139250-4 |D s |
| 689 | 0 | |8 1\p |5 DE-604 | |
| 689 | 1 | 0 | |a Biotechnologie |0 (DE-588)4069491-4 |D s |
| 689 | 1 | 1 | |a Nucleinsäuren |0 (DE-588)4172117-2 |D s |
| 689 | 1 | |8 2\p |5 DE-604 | |
| 689 | 2 | 0 | |a Proteindesign |0 (DE-588)4346801-9 |D s |
| 689 | 2 | |8 3\p |5 DE-604 | |
| 856 | 4 | 0 | |u http://search.ebscohost.com/login.aspx?direct=true&scope=site&db=nlebk&db=nlabk&AN=203205 |x Aggregator |3 Volltext |
| 912 | |a ZDB-4-EBA | ||
| 999 | |a oai:aleph.bib-bvb.de:BVB01-028469374 | ||
| 883 | 1 | |8 1\p |a cgwrk |d 20201028 |q DE-101 |u https://d-nb.info/provenance/plan#cgwrk | |
| 883 | 1 | |8 2\p |a cgwrk |d 20201028 |q DE-101 |u https://d-nb.info/provenance/plan#cgwrk | |
| 883 | 1 | |8 3\p |a cgwrk |d 20201028 |q DE-101 |u https://d-nb.info/provenance/plan#cgwrk | |
| 966 | e | |u http://search.ebscohost.com/login.aspx?direct=true&scope=site&db=nlebk&db=nlabk&AN=203205 |l FAW01 |p ZDB-4-EBA |q FAW_PDA_EBA |x Aggregator |3 Volltext | |
| 966 | e | |u http://search.ebscohost.com/login.aspx?direct=true&scope=site&db=nlebk&db=nlabk&AN=203205 |l FAW02 |p ZDB-4-EBA |q FAW_PDA_EBA |x Aggregator |3 Volltext | |
Datensatz im Suchindex
| _version_ | 1804175411071942656 |
|---|---|
| any_adam_object | |
| author | Hermanson, Greg T. |
| author_facet | Hermanson, Greg T. |
| author_role | aut |
| author_sort | Hermanson, Greg T. |
| author_variant | g t h gt gth |
| building | Verbundindex |
| bvnumber | BV043044836 |
| collection | ZDB-4-EBA |
| ctrlnum | (OCoLC)162128986 (DE-599)BVBBV043044836 |
| dewey-full | 574.19/296 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 574 - [Unassigned] |
| dewey-raw | 574.19/296 |
| dewey-search | 574.19/296 |
| dewey-sort | 3574.19 3296 |
| dewey-tens | 570 - Biology |
| discipline | Biologie |
| format | Electronic eBook |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>06060nmm a2200781zc 4500</leader><controlfield tag="001">BV043044836</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">20161017 </controlfield><controlfield tag="007">cr|uuu---uuuuu</controlfield><controlfield tag="008">151123s1996 |||| o||u| ||||||eng d</controlfield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">0080527906</subfield><subfield code="c">electronic bk.</subfield><subfield code="9">0-08-052790-6</subfield></datafield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">012342335X</subfield><subfield code="9">0-12-342335-X</subfield></datafield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">0123423368</subfield><subfield code="c">paper : alk. paper</subfield><subfield code="9">0-12-342336-8</subfield></datafield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">1281019607</subfield><subfield code="9">1-281-01960-7</subfield></datafield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">9780080527901</subfield><subfield code="c">electronic bk.</subfield><subfield code="9">978-0-08-052790-1</subfield></datafield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">9780123423351</subfield><subfield code="9">978-0-12-342335-1</subfield></datafield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">9780123423368</subfield><subfield code="c">paper : alk. paper</subfield><subfield code="9">978-0-12-342336-8</subfield></datafield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">9781281019608</subfield><subfield code="9">978-1-281-01960-8</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)162128986</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)BVBBV043044836</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">aacr</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-1046</subfield><subfield code="a">DE-1047</subfield></datafield><datafield tag="082" ind1="0" ind2=" "><subfield code="a">574.19/296</subfield><subfield code="2">22</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Hermanson, Greg T.</subfield><subfield code="e">Verfasser</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Bioconjugate techniques</subfield><subfield code="c">Greg T. Hermanson</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">San Diego</subfield><subfield code="b">Academic Press</subfield><subfield code="c">©1996</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">1 Online-Ressource (xxv, 785 pages)</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="500" ind1=" " ind2=" "><subfield code="a">Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002</subfield></datafield><datafield tag="500" ind1=" " ind2=" "><subfield code="a">Includes bibliographical references (pages 673-725) and index</subfield></datafield><datafield tag="500" ind1=" " ind2=" "><subfield code="a">Functional targets -- The chemistry of reactive groups -- Zero-length cross-linkers -- Homobifunctional cross-linkers -- Heterobifunctional cross-linkers -- Trifunctional cross-linkers -- Cleavable reagent systems -- Tags and probes -- Preparation of hapten-carrier immunogen conjugates -- Antibody modification and conjugation -- Immunotoxin conjugation techniques -- Preparation of liposome conjugates and derivatives -- Avidin-biotin systems -- Preparation of colloidal-gold-labeled proteins -- Modification with synthetic polymers -- Enzyme modification and conjugation -- Nucleic acid and oligonucleotide modification and conjugation</subfield></datafield><datafield tag="500" ind1=" " ind2=" "><subfield code="a">Bioconjugate Techniques is the essential guide to the modification and crosslinking of biomolecules for use in research, diagnostics, and therapeutics. It provides highly detailed information on the chemistry, reagent systems, and practical applications for creating labeled or conjugate molecules. It also describes dozens of reactions with details on hundreds of commercially available reagents and the use of these reagents for modifying or crosslinking peptides and proteins, sugars and polysaccharides, nucleic acids and oligonucleotides, lipids, and synthetic polymers. Armed with this information and the abundant protocols provided, readers will form unique complexes that can be used for detecting, quantifying, and targeting important analytes. This book helps readers make: high activity antibody-enzymes conjugates, immunotoxins, immunogen complexes, liposome conjugates; as well as biotinylated molecules, avidin or streptavidin conjugates, colloidal gold labeled proteins, PEG or dextran complexes, labeled oligonucleotide probes, and fluorescently tagged or radiolabeled molecules. Key Features * This book is the first to thoroughly capture the entire field of bioconjugate chemistry in a single volume * Serves as a practical guide to modification and cross-linking technology for research, diagnostics, and therapeutics * Provides useful, detailed, easy-to-follow, step-by-step protocols * Contains easy-to-read, and easy-to-understand key concepts for making bioconjugates of all types * Efficiently covers the chemistry of bioconjugation, the major reagents available for modification and cross-linking, and the application of these reagents to the synthesis of highly active conjugates * Cites over more than references keyed to concepts covered in the book * Uses more than 600 figures to illustrate bioconjugate reagents, their reactions, and applications * Suggests sources for all key reagents</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Bioconjugates / Synthesis. Biochemistry / methods. Proteins / chemistry. Peptides / chemistry. Nucleic Acids / chemistry</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">SCIENCE / Life Sciences / Biochemistry</subfield><subfield code="2">bisacsh</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Biochemie</subfield><subfield code="2">gtt</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Wetenschappelijke technieken</subfield><subfield code="2">gtt</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Biochemistry / methods</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Proteins / chemistry</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Peptides / chemistry</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Nucleic Acids / chemistry</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Wissenschaftliches Arbeiten</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Bioconjugates</subfield><subfield code="x">Synthesis</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Proteindesign</subfield><subfield code="0">(DE-588)4346801-9</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Nucleinsäuren</subfield><subfield code="0">(DE-588)4172117-2</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Biomolekül</subfield><subfield code="0">(DE-588)4135124-1</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Vernetzung</subfield><subfield code="g">Chemie</subfield><subfield code="0">(DE-588)4139250-4</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Biotechnologie</subfield><subfield code="0">(DE-588)4069491-4</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Konjugate</subfield><subfield code="0">(DE-588)4131219-3</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="689" ind1="0" ind2="0"><subfield code="a">Konjugate</subfield><subfield code="0">(DE-588)4131219-3</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="1"><subfield code="a">Biomolekül</subfield><subfield code="0">(DE-588)4135124-1</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="2"><subfield code="a">Vernetzung</subfield><subfield code="g">Chemie</subfield><subfield code="0">(DE-588)4139250-4</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2=" "><subfield code="8">1\p</subfield><subfield code="5">DE-604</subfield></datafield><datafield tag="689" ind1="1" ind2="0"><subfield code="a">Biotechnologie</subfield><subfield code="0">(DE-588)4069491-4</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="1" ind2="1"><subfield code="a">Nucleinsäuren</subfield><subfield code="0">(DE-588)4172117-2</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="1" ind2=" "><subfield code="8">2\p</subfield><subfield code="5">DE-604</subfield></datafield><datafield tag="689" ind1="2" ind2="0"><subfield code="a">Proteindesign</subfield><subfield code="0">(DE-588)4346801-9</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="2" ind2=" "><subfield code="8">3\p</subfield><subfield code="5">DE-604</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">http://search.ebscohost.com/login.aspx?direct=true&scope=site&db=nlebk&db=nlabk&AN=203205</subfield><subfield code="x">Aggregator</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-4-EBA</subfield></datafield><datafield tag="999" ind1=" " ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-028469374</subfield></datafield><datafield tag="883" ind1="1" ind2=" "><subfield code="8">1\p</subfield><subfield code="a">cgwrk</subfield><subfield code="d">20201028</subfield><subfield code="q">DE-101</subfield><subfield code="u">https://d-nb.info/provenance/plan#cgwrk</subfield></datafield><datafield tag="883" ind1="1" ind2=" "><subfield code="8">2\p</subfield><subfield code="a">cgwrk</subfield><subfield code="d">20201028</subfield><subfield code="q">DE-101</subfield><subfield code="u">https://d-nb.info/provenance/plan#cgwrk</subfield></datafield><datafield tag="883" ind1="1" ind2=" "><subfield code="8">3\p</subfield><subfield code="a">cgwrk</subfield><subfield code="d">20201028</subfield><subfield code="q">DE-101</subfield><subfield code="u">https://d-nb.info/provenance/plan#cgwrk</subfield></datafield><datafield tag="966" ind1="e" ind2=" "><subfield code="u">http://search.ebscohost.com/login.aspx?direct=true&scope=site&db=nlebk&db=nlabk&AN=203205</subfield><subfield code="l">FAW01</subfield><subfield code="p">ZDB-4-EBA</subfield><subfield code="q">FAW_PDA_EBA</subfield><subfield code="x">Aggregator</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="966" ind1="e" ind2=" "><subfield code="u">http://search.ebscohost.com/login.aspx?direct=true&scope=site&db=nlebk&db=nlabk&AN=203205</subfield><subfield code="l">FAW02</subfield><subfield code="p">ZDB-4-EBA</subfield><subfield code="q">FAW_PDA_EBA</subfield><subfield code="x">Aggregator</subfield><subfield code="3">Volltext</subfield></datafield></record></collection> |
| id | DE-604.BV043044836 |
| illustrated | Not Illustrated |
| indexdate | 2024-07-10T07:15:51Z |
| institution | BVB |
| isbn | 0080527906 012342335X 0123423368 1281019607 9780080527901 9780123423351 9780123423368 9781281019608 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-028469374 |
| oclc_num | 162128986 |
| open_access_boolean | |
| owner | DE-1046 DE-1047 |
| owner_facet | DE-1046 DE-1047 |
| physical | 1 Online-Ressource (xxv, 785 pages) |
| psigel | ZDB-4-EBA ZDB-4-EBA FAW_PDA_EBA |
| publishDate | 1996 |
| publishDateSearch | 1996 |
| publishDateSort | 1996 |
| publisher | Academic Press |
| record_format | marc |
| spelling | Hermanson, Greg T. Verfasser aut Bioconjugate techniques Greg T. Hermanson San Diego Academic Press ©1996 1 Online-Ressource (xxv, 785 pages) txt rdacontent c rdamedia cr rdacarrier Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002 Includes bibliographical references (pages 673-725) and index Functional targets -- The chemistry of reactive groups -- Zero-length cross-linkers -- Homobifunctional cross-linkers -- Heterobifunctional cross-linkers -- Trifunctional cross-linkers -- Cleavable reagent systems -- Tags and probes -- Preparation of hapten-carrier immunogen conjugates -- Antibody modification and conjugation -- Immunotoxin conjugation techniques -- Preparation of liposome conjugates and derivatives -- Avidin-biotin systems -- Preparation of colloidal-gold-labeled proteins -- Modification with synthetic polymers -- Enzyme modification and conjugation -- Nucleic acid and oligonucleotide modification and conjugation Bioconjugate Techniques is the essential guide to the modification and crosslinking of biomolecules for use in research, diagnostics, and therapeutics. It provides highly detailed information on the chemistry, reagent systems, and practical applications for creating labeled or conjugate molecules. It also describes dozens of reactions with details on hundreds of commercially available reagents and the use of these reagents for modifying or crosslinking peptides and proteins, sugars and polysaccharides, nucleic acids and oligonucleotides, lipids, and synthetic polymers. Armed with this information and the abundant protocols provided, readers will form unique complexes that can be used for detecting, quantifying, and targeting important analytes. This book helps readers make: high activity antibody-enzymes conjugates, immunotoxins, immunogen complexes, liposome conjugates; as well as biotinylated molecules, avidin or streptavidin conjugates, colloidal gold labeled proteins, PEG or dextran complexes, labeled oligonucleotide probes, and fluorescently tagged or radiolabeled molecules. Key Features * This book is the first to thoroughly capture the entire field of bioconjugate chemistry in a single volume * Serves as a practical guide to modification and cross-linking technology for research, diagnostics, and therapeutics * Provides useful, detailed, easy-to-follow, step-by-step protocols * Contains easy-to-read, and easy-to-understand key concepts for making bioconjugates of all types * Efficiently covers the chemistry of bioconjugation, the major reagents available for modification and cross-linking, and the application of these reagents to the synthesis of highly active conjugates * Cites over more than references keyed to concepts covered in the book * Uses more than 600 figures to illustrate bioconjugate reagents, their reactions, and applications * Suggests sources for all key reagents Bioconjugates / Synthesis. Biochemistry / methods. Proteins / chemistry. Peptides / chemistry. Nucleic Acids / chemistry SCIENCE / Life Sciences / Biochemistry bisacsh Biochemie gtt Wetenschappelijke technieken gtt Biochemistry / methods Proteins / chemistry Peptides / chemistry Nucleic Acids / chemistry Wissenschaftliches Arbeiten Bioconjugates Synthesis Proteindesign (DE-588)4346801-9 gnd rswk-swf Nucleinsäuren (DE-588)4172117-2 gnd rswk-swf Biomolekül (DE-588)4135124-1 gnd rswk-swf Vernetzung Chemie (DE-588)4139250-4 gnd rswk-swf Biotechnologie (DE-588)4069491-4 gnd rswk-swf Konjugate (DE-588)4131219-3 gnd rswk-swf Konjugate (DE-588)4131219-3 s Biomolekül (DE-588)4135124-1 s Vernetzung Chemie (DE-588)4139250-4 s 1\p DE-604 Biotechnologie (DE-588)4069491-4 s Nucleinsäuren (DE-588)4172117-2 s 2\p DE-604 Proteindesign (DE-588)4346801-9 s 3\p DE-604 http://search.ebscohost.com/login.aspx?direct=true&scope=site&db=nlebk&db=nlabk&AN=203205 Aggregator Volltext 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk 2\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk 3\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Hermanson, Greg T. Bioconjugate techniques Bioconjugates / Synthesis. Biochemistry / methods. Proteins / chemistry. Peptides / chemistry. Nucleic Acids / chemistry SCIENCE / Life Sciences / Biochemistry bisacsh Biochemie gtt Wetenschappelijke technieken gtt Biochemistry / methods Proteins / chemistry Peptides / chemistry Nucleic Acids / chemistry Wissenschaftliches Arbeiten Bioconjugates Synthesis Proteindesign (DE-588)4346801-9 gnd Nucleinsäuren (DE-588)4172117-2 gnd Biomolekül (DE-588)4135124-1 gnd Vernetzung Chemie (DE-588)4139250-4 gnd Biotechnologie (DE-588)4069491-4 gnd Konjugate (DE-588)4131219-3 gnd |
| subject_GND | (DE-588)4346801-9 (DE-588)4172117-2 (DE-588)4135124-1 (DE-588)4139250-4 (DE-588)4069491-4 (DE-588)4131219-3 |
| title | Bioconjugate techniques |
| title_auth | Bioconjugate techniques |
| title_exact_search | Bioconjugate techniques |
| title_full | Bioconjugate techniques Greg T. Hermanson |
| title_fullStr | Bioconjugate techniques Greg T. Hermanson |
| title_full_unstemmed | Bioconjugate techniques Greg T. Hermanson |
| title_short | Bioconjugate techniques |
| title_sort | bioconjugate techniques |
| topic | Bioconjugates / Synthesis. Biochemistry / methods. Proteins / chemistry. Peptides / chemistry. Nucleic Acids / chemistry SCIENCE / Life Sciences / Biochemistry bisacsh Biochemie gtt Wetenschappelijke technieken gtt Biochemistry / methods Proteins / chemistry Peptides / chemistry Nucleic Acids / chemistry Wissenschaftliches Arbeiten Bioconjugates Synthesis Proteindesign (DE-588)4346801-9 gnd Nucleinsäuren (DE-588)4172117-2 gnd Biomolekül (DE-588)4135124-1 gnd Vernetzung Chemie (DE-588)4139250-4 gnd Biotechnologie (DE-588)4069491-4 gnd Konjugate (DE-588)4131219-3 gnd |
| topic_facet | Bioconjugates / Synthesis. Biochemistry / methods. Proteins / chemistry. Peptides / chemistry. Nucleic Acids / chemistry SCIENCE / Life Sciences / Biochemistry Biochemie Wetenschappelijke technieken Biochemistry / methods Proteins / chemistry Peptides / chemistry Nucleic Acids / chemistry Wissenschaftliches Arbeiten Bioconjugates Synthesis Proteindesign Nucleinsäuren Biomolekül Vernetzung Chemie Biotechnologie Konjugate |
| url | http://search.ebscohost.com/login.aspx?direct=true&scope=site&db=nlebk&db=nlabk&AN=203205 |
| work_keys_str_mv | AT hermansongregt bioconjugatetechniques |