RNA methodologies: a laboratory guide for isolation and characterization
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Amsterdam
Elsevier/Academic Press
c2005
|
| Ausgabe: | 3rd ed |
| Schlagworte: | |
| Online-Zugang: | FAW01 FAW02 Volltext |
| Beschreibung: | Includes bibliographical references and index RNA and the cellular biochemistry revisited -- Transcription and the organization of eukaryotic genes -- Messenger RNA -- Resilient ribonucleases -- RNA isolation strategies -- The truth about tissues -- Isolation of polyadenylated RNA -- Quality control for RNA preparations -- Dot blot analysis -- Electrophoresis of RNA -- Photodocumentation and image analysis -- Northern analysis -- Nucleic acid probe technology -- Practical nucleic acid hybridization -- Principles of detection -- Quantification of specific mRNAs by nuclease protection -- Analysis of nuclear RNA -- cDNA synthesis -- RT-PCR -- Quantitative PCR techniques -- Transcript subtraction methods -- mRNA differential display -- High-throughput analysis of gene expression -- RNA interference : targeted gene silencing -- Genomes, transcriptomes, proteomes, and bioinformatics -- An RNA Paradigm -- Epilogue : a few pearls of wisdom This laboratory guide represents a growing collection of tried, tested and optimized laboratory protocols for the isolation and characterization of eukaryotic RNA, with lesser emphasis on the characterization of prokaryotic transcripts. Collectively the chapters work together to embellish the RNA story, each presenting clear take-home lessons, liberally incorporating flow charts, tables and graphs to facilitate learning and assist in the planning and implementation phases of a project. RNA Methodologies, 3rd edition includes approximately 30% new material, including chapters on the more recent technologies of RNA interference including: RNAi; Microarrays; Bioinformatics. It also includes new sections on: new and improved RT-PCR techniques; innovative 5 and 3 RACE techniques; subtractive PCR methods; methods for improving cDNA synthesis. * Author is a well-recognized expert in the field of RNA experimentation and founded Exon-Intron, a well-known biotechnology educational workshop center * Includes classic and contemporary techniques * Incorporates flow charts, tables, and graphs to facilitate learning and assist in the planning phases of projects |
| Beschreibung: | 1 Online-Ressource (xxv, 767 p.) |
| ISBN: | 0080454763 0122496965 9780080454764 9780122496967 |
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Datensatz im Suchindex
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| author | Farrell, Robert E. |
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| discipline | Biologie |
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| indexdate | 2024-07-10T07:15:51Z |
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| isbn | 0080454763 0122496965 9780080454764 9780122496967 |
| language | English |
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| spelling | Farrell, Robert E. Verfasser aut RNA methodologies a laboratory guide for isolation and characterization Robert E. Farrell, Jr 3rd ed Amsterdam Elsevier/Academic Press c2005 1 Online-Ressource (xxv, 767 p.) txt rdacontent c rdamedia cr rdacarrier Includes bibliographical references and index RNA and the cellular biochemistry revisited -- Transcription and the organization of eukaryotic genes -- Messenger RNA -- Resilient ribonucleases -- RNA isolation strategies -- The truth about tissues -- Isolation of polyadenylated RNA -- Quality control for RNA preparations -- Dot blot analysis -- Electrophoresis of RNA -- Photodocumentation and image analysis -- Northern analysis -- Nucleic acid probe technology -- Practical nucleic acid hybridization -- Principles of detection -- Quantification of specific mRNAs by nuclease protection -- Analysis of nuclear RNA -- cDNA synthesis -- RT-PCR -- Quantitative PCR techniques -- Transcript subtraction methods -- mRNA differential display -- High-throughput analysis of gene expression -- RNA interference : targeted gene silencing -- Genomes, transcriptomes, proteomes, and bioinformatics -- An RNA Paradigm -- Epilogue : a few pearls of wisdom This laboratory guide represents a growing collection of tried, tested and optimized laboratory protocols for the isolation and characterization of eukaryotic RNA, with lesser emphasis on the characterization of prokaryotic transcripts. Collectively the chapters work together to embellish the RNA story, each presenting clear take-home lessons, liberally incorporating flow charts, tables and graphs to facilitate learning and assist in the planning and implementation phases of a project. RNA Methodologies, 3rd edition includes approximately 30% new material, including chapters on the more recent technologies of RNA interference including: RNAi; Microarrays; Bioinformatics. It also includes new sections on: new and improved RT-PCR techniques; innovative 5 and 3 RACE techniques; subtractive PCR methods; methods for improving cDNA synthesis. * Author is a well-recognized expert in the field of RNA experimentation and founded Exon-Intron, a well-known biotechnology educational workshop center * Includes classic and contemporary techniques * Incorporates flow charts, tables, and graphs to facilitate learning and assist in the planning phases of projects ARN / Analyse / Manuels de laboratoire SCIENCE / Life Sciences / Molecular Biology bisacsh RNA. gtt Laboratoriumonderzoek gtt RNA / Analysis fast RNA / Laboratory Manuals / isolation & purification Molecular Biology / Laboratory Manuals / methods RNA / Laboratory Manuals / physiology RNA Analysis Laboratory manuals Methode (DE-588)4038971-6 gnd rswk-swf Isolierung (DE-588)4027787-2 gnd rswk-swf RNS (DE-588)4076759-0 gnd rswk-swf Isolierung Mikrobiologie (DE-588)4193882-3 gnd rswk-swf Isolierung Chemie (DE-588)4122223-4 gnd rswk-swf RNS (DE-588)4076759-0 s Isolierung (DE-588)4027787-2 s Methode (DE-588)4038971-6 s 1\p DE-604 Isolierung Mikrobiologie (DE-588)4193882-3 s 2\p DE-604 Isolierung Chemie (DE-588)4122223-4 s 3\p DE-604 http://search.ebscohost.com/login.aspx?direct=true&scope=site&db=nlebk&db=nlabk&AN=166200 Aggregator Volltext 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk 2\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk 3\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Farrell, Robert E. RNA methodologies a laboratory guide for isolation and characterization ARN / Analyse / Manuels de laboratoire SCIENCE / Life Sciences / Molecular Biology bisacsh RNA. gtt Laboratoriumonderzoek gtt RNA / Analysis fast RNA / Laboratory Manuals / isolation & purification Molecular Biology / Laboratory Manuals / methods RNA / Laboratory Manuals / physiology RNA Analysis Laboratory manuals Methode (DE-588)4038971-6 gnd Isolierung (DE-588)4027787-2 gnd RNS (DE-588)4076759-0 gnd Isolierung Mikrobiologie (DE-588)4193882-3 gnd Isolierung Chemie (DE-588)4122223-4 gnd |
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| title | RNA methodologies a laboratory guide for isolation and characterization |
| title_auth | RNA methodologies a laboratory guide for isolation and characterization |
| title_exact_search | RNA methodologies a laboratory guide for isolation and characterization |
| title_full | RNA methodologies a laboratory guide for isolation and characterization Robert E. Farrell, Jr |
| title_fullStr | RNA methodologies a laboratory guide for isolation and characterization Robert E. Farrell, Jr |
| title_full_unstemmed | RNA methodologies a laboratory guide for isolation and characterization Robert E. Farrell, Jr |
| title_short | RNA methodologies |
| title_sort | rna methodologies a laboratory guide for isolation and characterization |
| title_sub | a laboratory guide for isolation and characterization |
| topic | ARN / Analyse / Manuels de laboratoire SCIENCE / Life Sciences / Molecular Biology bisacsh RNA. gtt Laboratoriumonderzoek gtt RNA / Analysis fast RNA / Laboratory Manuals / isolation & purification Molecular Biology / Laboratory Manuals / methods RNA / Laboratory Manuals / physiology RNA Analysis Laboratory manuals Methode (DE-588)4038971-6 gnd Isolierung (DE-588)4027787-2 gnd RNS (DE-588)4076759-0 gnd Isolierung Mikrobiologie (DE-588)4193882-3 gnd Isolierung Chemie (DE-588)4122223-4 gnd |
| topic_facet | ARN / Analyse / Manuels de laboratoire SCIENCE / Life Sciences / Molecular Biology RNA. Laboratoriumonderzoek RNA / Analysis RNA / Laboratory Manuals / isolation & purification Molecular Biology / Laboratory Manuals / methods RNA / Laboratory Manuals / physiology RNA Analysis Laboratory manuals Methode Isolierung RNS Isolierung Mikrobiologie Isolierung Chemie |
| url | http://search.ebscohost.com/login.aspx?direct=true&scope=site&db=nlebk&db=nlabk&AN=166200 |
| work_keys_str_mv | AT farrellroberte rnamethodologiesalaboratoryguideforisolationandcharacterization |