Detection challenges in clinical diagnostics:
Saved in:
| Other Authors: | |
|---|---|
| Format: | Electronic eBook |
| Language: | English |
| Published: |
Cambridge
Royal Society of Chemistry
[2013]
|
| Series: | RSC detection science series
no. 2 |
| Subjects: | |
| Online Access: | FAW01 FAW02 Volltext Inhaltsverzeichnis |
| Item Description: | Print version record |
| Physical Description: | 1 online resource |
| ISBN: | 184973612X 1849737304 9781849736121 9781849737302 |
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| 505 | 8 | |a There are many remaining challenges impeding future progress in field of Clinical Diagnostics. This book presents a technical assessment and vision of clinical leaders, scoping the clinical and other diagnostic needs and the bottle-necks in their cognate fields. Issues of real environmental biological measurements from the perspective of the end-user are presented and thus the book serves to inform the direction of the fundamental scientific efforts. Both editors are experienced practitioners within the biosensor technology and are involved first-hand with the healthcare and clinical applications of detection science | |
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| 650 | 4 | |a Biomedical engineering | |
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| adam_text | Titel: Detection challenges in clinical diagnostics
Autor: Vadgama, Pankaj
Jahr: 2013
Contents
List of Contributors xvii
Chapter 1 Biosensor Technology and the Clinical Biochemistry
Laboratory - Issue of Signal Interference from the Biological
Matrix 1
Michael Thompson, Sonia Sheikh, Christophe Blaszykowski
and Alexander Romaschin
1.1 Laboratory Clinical Biochemical Assays 1
1.2 Biosensor Technology 6
1.2.1 Biosensor Architecture 6
1.2.2 Probe Attachment to Device Surfaces 7
1.2.3 Devices and Transduction 11
1.3 Biosensors and Measurement of Clinical Targets 21
1.4 Signal Interference and the Non-specific Adsorption
Problem 23
1.5 A Look at Surface Chemistries to Solve the NSA Issue 29
1.6 A Final Comment 30
Acknowledgements 31
References 32
Chapter 2 Integrated Chemistries for Analytical Simplification
and Point of Care Testing 35
Pankaj Vadgama, Salzitsa Anastasova and
Anna Spehar-Deleze
2.1 Introduction 35
2.2 Fluidics for POCT 36
RSC Detection Science Series No. 2
Detection Challenges in Clinical Diagnostics
Edited by Pankaj Vadgama and Serban Peteu
© The Royal Society of Chemistry 2013
Published by the Royal Society of Chemistry, www.rsc.org
xi
xii Contents
2.3 Lateral Flow Techniques - From Simple Colorimetric
Strips to 3D Flow 40
2.3.1 Colorimetric Strips 40
2.3.2 Nanoparticle Labels 41
2.3.3 New Opportunities 43
2.4 Biological Recognition 43
2.4.1 Immobilisation Techniques 43
2.4.2 Enzyme-Based Systems 47
2.4.3 Aptamers as Biorecognition Elements 48
2.5 Electrochemical Sensors 50
2.5.1 Ion-Selective Electrodes 50
2.5.2 Amperometric Enzyme Biosensors 52
2.5.3 Immunosensors 54
2.5.4 DNA Sensors 56
2.6 Micromechanical Transduction 56
2.7 Commercialisation 57
2.8 Conclusions 58
Acknowledgement 59
References 59
Chapter 3 Blood-Glucose Biosensors, Development
and Challenges 65
Yuan Wang and Madeleine Hu
3.1 Introduction 65
3.2 History of Blood-Glucose Biosensor 66
3.2.1 First Generation of Biosensors 67
3.2.2 Second Generation of Glucose
Biosensors 68
3.2.3 Third Generation of Glucose
Biosensor 69
3.3 Sensors for BGMS 69
3.3.1 Electrode 70
3.3.2 Reaction Chamber 72
3.3.3 Chemistry 73
3.3.4 Detection Method 74
3.3.5 Correction Algorithm 74
3.3.6 Calibration of BGMS 76
3.3.7 Performance Validation of BGMS 77
3.3.8 Manufacturing Process 79
3.4 Clinical Utility and Potential Problems 80
3.5 Continuous Glucose Monitoring System
(CGMS) 81
References 82
Contents
Xlll
Chapter 4 Recent Progress in the Electrochemical Detection of
Disease-Related Diagnostic Biomarkers 89
Alina Vasilescu, Wolfgang Schuhmann and Szilveszter Gàspâr
4.1 Introduction 89
4.2 Electrochemical Sensors for Detection of Cancer
Biomarkers 92
4.2.1 Electrochemical Sensors for
Carcinoembryonic Antigen 92
4.2.2 Electrochemical Sensors for Prostate-Specific
Antigen 93
4.2.3 Electrochemical Sensors for Other Protein
Cancer Biomarkers 94
4.2.4 Electrochemical Sensors for Simultaneous
Detection of Several Protein Biomarkers 96
4.2.5 Electrochemical Sensors for Genetic Markers
of Cancer 98
4.2.6 Electrochemical Sensors for Detection of
Cancer Cells 100
4.3 Electrochemical Sensors for Detection of Cardiac
Biomarkers 103
4.4 Electrochemical Sensors for Detection of Acquired
Immunodeficiency Syndrome 107
4.5 Electrochemical Sensors for the Detection of
Hepatitis Biomarkers 110
4.5.1 Electrochemical Sensors for Hepatitis B 110
4.5.2 Electrochemical Sensors for Hepatitis C 113
4.6 Electrochemical Sensors for the Detection of
Rheumatoid Arthritis Biomarkers 115
4.7 Electrochemical Sensors for the Detection of Celiac
Disease Biomarkers 116
4.8 Electrochemical Sensors for the Detection of Urinary
Tract Infection Biomarkers 117
4.9 Challenges in the Use of Electrochemical Sensors in
Diagnostics 121
4.10 Conclusions 122
References 122
Chapter 5 In Vivo Sensors for Continuous Monitoring of Blood Gases,
Glucose, and Lactate: Biocompatibility Challenges and
Potential Solutions 129
Megan C. Frost, Alexander K. Wolf and Mark E. Mey erhoff
5.1 Introduction
5.2 Design of In Vivo Sensors
129
130
xiv
Contents
5.2.1 Sensing/ 02// C02/pH in Blood 130
5.2.2 Sensing Glucose in Subcutaneous
Tissue 134
5.2.3 Sensing Lactate in Blood 137
5.3 Biocompatibility Issues that Influence In Vivo Sensor
Performance and Reliability 138
5.3.1 Details of Biological Response in Blood 139
5.3.2 Details of Biological Response in
Subcutaneous Tissue 140
5.4 Strategies for Mediating Biological Response to
Implanted Sensors 143
5.4.1 Materials Development for Improved
Biological Response to Implanted
Sensors 143
5.4.2 Active Releasing Materials for Controlling
Biological Response 144
5.4.3 Materials to Mimic Biological Form and
Function 150
5.5 Summary 150
References 151
Chapter 6 Peroxynitrite Electrochemical Quantification: Recent
Advances and Challenges 156
Serban F. Peteu and Sabine Szunerits
6.1 Introduction 156
6.2 Challenges Confronted in the Accurate Quantification
of Peroxynitrite 159
6.3 Electrochemical Interfaces and Methods for
Peroxynitrite Detection 163
6.4 Strategies to Increase Response Sensitivity:
Electroactive Polymers or Graphene 169
6.5 Conclusions and Perspectives 174
Acknowledgements 176
References 176
Chapter 7 The Diagnosis of Myelodysplasie Syndromes 182
Alison S. Thomas and Christopher McNamara
7.1 Morphological Features of Myelodysplasia 183
7.1.1 Erythroid Dysplasia 183
7.1.2 Granulocytic Dysplasia 184
7.1.3 Megakaryocytic Dysplasia 184
Contents xv
7.2 Differential Diagnosis of Myelodysplasia 186
7.2.1 Metabolic Abnormalities 186
7.2.2 Drugs and Toxins 186
7.2.3 Infectious Diseases 187
7.2.4 Congenital Disorders 187
7.2.5 Acute Myeloid Leukaemia and Other
Haematopoietic Neoplasms 187
7.3 Initial Assessment of a Patient with Possible MDS 188
7.4 Additional Diagnostic Tools for MDS 188
7.4.1 Bone Marrow Aspirate Cytochemistry 188
7.4.2 Bone Marrow Trephine Biopsy 188
7.4.3 Cytogenetics 190
7.4.4 Immunophenotyping 191
7.5 Diagnostic Difficulties in MDS 191
7.5.1 Unexplained Cytopenias in the Presence of
Minimal Dysplasia 191
7.5.2 Myelioproliferative/Myelodysplastic Overlap
Syndromes 192
7.5.3 Cytopenias in the Presence of a Hypocellular
Bone Marrow 192
7.5.4 Dysplasia in the Presence of Bone Marrow
Fibrosis 192
7.6 Subclassification of MDS 193
7.6.1 Original FAB Classification 193
7.6.2 WHO Classification 3rd Edition and
4th Edition 194
7.7 Evolving Understanding of the Pathophysiology
of MDS 194
7.7.1 Altered Methylation 195
7.7.2 Abnormalities in RNA Splicing Machinery 196
References 196
Chapter 8 The Prospects for Real-Time Raman Spectroscopy for
Oesophageal Neoplasia 201
Max Almond, Gavin Rhys-Lloyd, Jo Hutchings,
Geeta Shetty, Neil Shepherd, Catherine Kendall,
Nicholas Stone and Hugh Barr
8.1 The Clinical Problem 201
8.2 Endoscopic Recognition of Dysplasia and Cancer 202
8.3 Raman Spectroscopy 204
8.4 Instrumentation 206
8.5 Raman Spectroscopy for Medical Diagnosis 207
8.6 Raman Spectroscopy Assessment of Oesophageal
Pathology 208
xvi Contents
8.7 Fibre-Optic Raman Spectroscopy 210
8.8 Novel Raman Probe 213
8.9 Discussion 215
References 218
Chapter 9 Volatile Analysis for Clinical Diagnostics 222
Catherine Kendall, Hugh Barr and Naresh Magan
9.1 Volatile Diagnostics 222
9.1.1 GC-MS and SIFT-MS Techniques 223
9.1.2 Electronic Noses 224
9.2 Applications of Volatile Fingerprinting 227
9.2.1 Food 227
9.2.2 Medical Applications 228
9.2.3 Environmental 231
9.3 Ventilator-Associated Pneumonia (VAP) 231
Acknowledgements 233
References 233
Subject Index
238
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| contents | There are many remaining challenges impeding future progress in field of Clinical Diagnostics. This book presents a technical assessment and vision of clinical leaders, scoping the clinical and other diagnostic needs and the bottle-necks in their cognate fields. Issues of real environmental biological measurements from the perspective of the end-user are presented and thus the book serves to inform the direction of the fundamental scientific efforts. Both editors are experienced practitioners within the biosensor technology and are involved first-hand with the healthcare and clinical applications of detection science |
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| spelling | Detection challenges in clinical diagnostics edited by Pankaj Vadgama [and others] Cambridge Royal Society of Chemistry [2013] © 2013 1 online resource txt rdacontent c rdamedia cr rdacarrier RSC detection science series no. 2 Print version record There are many remaining challenges impeding future progress in field of Clinical Diagnostics. This book presents a technical assessment and vision of clinical leaders, scoping the clinical and other diagnostic needs and the bottle-necks in their cognate fields. Issues of real environmental biological measurements from the perspective of the end-user are presented and thus the book serves to inform the direction of the fundamental scientific efforts. Both editors are experienced practitioners within the biosensor technology and are involved first-hand with the healthcare and clinical applications of detection science HEALTH & FITNESS / Diseases / General bisacsh MEDICAL / Clinical Medicine bisacsh MEDICAL / Diseases bisacsh MEDICAL / Evidence-Based Medicine bisacsh MEDICAL / Internal Medicine bisacsh Evidenz-basierte Medizin Innere Medizin Medizin Clinical biochemistry Clinical chemistry Diagnosis, Laboratory Biomedical engineering Biosensors Diagnosis, Noninvasive Vadgama, Pankaj edt http://search.ebscohost.com/login.aspx?direct=true&scope=site&db=nlebk&db=nlabk&AN=706406 Aggregator Volltext HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=028455791&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Detection challenges in clinical diagnostics There are many remaining challenges impeding future progress in field of Clinical Diagnostics. This book presents a technical assessment and vision of clinical leaders, scoping the clinical and other diagnostic needs and the bottle-necks in their cognate fields. Issues of real environmental biological measurements from the perspective of the end-user are presented and thus the book serves to inform the direction of the fundamental scientific efforts. Both editors are experienced practitioners within the biosensor technology and are involved first-hand with the healthcare and clinical applications of detection science HEALTH & FITNESS / Diseases / General bisacsh MEDICAL / Clinical Medicine bisacsh MEDICAL / Diseases bisacsh MEDICAL / Evidence-Based Medicine bisacsh MEDICAL / Internal Medicine bisacsh Evidenz-basierte Medizin Innere Medizin Medizin Clinical biochemistry Clinical chemistry Diagnosis, Laboratory Biomedical engineering Biosensors Diagnosis, Noninvasive |
| title | Detection challenges in clinical diagnostics |
| title_auth | Detection challenges in clinical diagnostics |
| title_exact_search | Detection challenges in clinical diagnostics |
| title_full | Detection challenges in clinical diagnostics edited by Pankaj Vadgama [and others] |
| title_fullStr | Detection challenges in clinical diagnostics edited by Pankaj Vadgama [and others] |
| title_full_unstemmed | Detection challenges in clinical diagnostics edited by Pankaj Vadgama [and others] |
| title_short | Detection challenges in clinical diagnostics |
| title_sort | detection challenges in clinical diagnostics |
| topic | HEALTH & FITNESS / Diseases / General bisacsh MEDICAL / Clinical Medicine bisacsh MEDICAL / Diseases bisacsh MEDICAL / Evidence-Based Medicine bisacsh MEDICAL / Internal Medicine bisacsh Evidenz-basierte Medizin Innere Medizin Medizin Clinical biochemistry Clinical chemistry Diagnosis, Laboratory Biomedical engineering Biosensors Diagnosis, Noninvasive |
| topic_facet | HEALTH & FITNESS / Diseases / General MEDICAL / Clinical Medicine MEDICAL / Diseases MEDICAL / Evidence-Based Medicine MEDICAL / Internal Medicine Evidenz-basierte Medizin Innere Medizin Medizin Clinical biochemistry Clinical chemistry Diagnosis, Laboratory Biomedical engineering Biosensors Diagnosis, Noninvasive |
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