Synthetic biology: a lab manual
Saved in:
| Main Author: | |
|---|---|
| Format: | Electronic eBook |
| Language: | English |
| Published: |
Hackensack, NJ
World Scientific Publishing Company
2014
|
| Subjects: | |
| Online Access: | FAW01 FAW02 Volltext |
| Item Description: | Online resource; title from PDF title page (Ebsco; viewed on May 21, 2014) |
| Physical Description: | 1 online resource |
| ISBN: | 9789814579551 9814579556 |
Staff View
MARC
| LEADER | 00000nmm a2200000zc 4500 | ||
|---|---|---|---|
| 001 | BV043027369 | ||
| 003 | DE-604 | ||
| 005 | 00000000000000.0 | ||
| 007 | cr|uuu---uuuuu | ||
| 008 | 151120s2014 |||| o||u| ||||||eng d | ||
| 020 | |a 9789814579551 |9 978-981-4579-55-1 | ||
| 020 | |a 9814579556 |9 981-4579-55-6 | ||
| 035 | |a (OCoLC)880354187 | ||
| 035 | |a (DE-599)BVBBV043027369 | ||
| 040 | |a DE-604 |b ger |e rda | ||
| 041 | 0 | |a eng | |
| 049 | |a DE-1046 |a DE-1047 | ||
| 082 | 0 | |a 660.6 |2 23 | |
| 100 | 1 | |a Liljeruhm, Josefine |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a Synthetic biology |b a lab manual |c Josefine Liljeruhm, Erik Gullberg, Anthony C. Forster, Uppsala University, Sweden |
| 264 | 1 | |a Hackensack, NJ |b World Scientific Publishing Company |c 2014 | |
| 300 | |a 1 online resource | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b c |2 rdamedia | ||
| 338 | |b cr |2 rdacarrier | ||
| 500 | |a Online resource; title from PDF title page (Ebsco; viewed on May 21, 2014) | ||
| 505 | 8 | |a 1. Introduction. What is synthetic biology, exactly? The iGEM outbreak. A synthetic biology lab manual -- 2. Genes, chromoproteins and antisense RNAs. E. coli DNA: Chromosomes, plasmids and copy number. Coupling of transcription and translation in bacteria. Promoter and terminator for transcription. Ribosome binding site (RBS). Codon bias. Chromoproteins. Small regulatory RNAs (sRNAs) -- 3. Lab rooms and equipment. The physical lab spaces. Equipment -- 4. Safety is priority #1. Fires. Chemicals. Biological safety and disposal. Dangerous equipment -- 5. Lab course projects. Time and resources. Project overview and learning objectives. The lab notebook. Lab section 1. Preparation of chemical solutions and agar plates. Lab section 2. Coloring bacteria by adding a promoter to a chromoprotein gene. Lab section 3: Rational engineering of chromoprotein expression level. Lab section 4. Other experiments. The "dreaded" exam -- 6. Protocols. Introduction. Protocol 1. Preparation of solutions and agar plates. Protocol 2. Overnight cultures with antibiotics, and glycerol stocks. Protocol 3. BioBrick 3A assembly and gel analysis. Protocol 4. Agarose gel electrophoresis. Protocol 5. Preparation of competent E. coli cells using CaCl2. Protocol 6. Transformation of CaCl2-competent E. coli cells. Protocol 7. Bacterial re-streaking techniques. Protocol 8. Lysis of E. coli cells with lysozyme. Protocol 9. Polymerase chain reaction (PCR). Protocol 10. Inverse PCR mutagenesis. Protocol 11. Colony PCR. Protocol 12. Gibson assembly -- 7. Advanced methods. Flow cytometry and cell sorting. Recombination in plasmids and the chromosome. Electrocompetent cells -- 8. The International Genetically Engineered Machine (iGEM) Competition. How to start an iGEM team. Uppsala iGEM 2011 -- Show color with color. Uppsala iGEM 2012 -- Resistance is futile. Uppsala iGEM 2013 -- Lactonutritious -- it's delicious -- 9. Appendices | |
| 505 | 8 | |a Synthetic Biology: A Lab Manual is the first manual for laboratory work in the new and rapidly expanding field of synthetic biology. Aimed at non-specialists, it details protocols central to synthetic biology in both education and research. In addition, it provides all the information that teachers and students from high schools and tertiary institutions need for a colorful lab course in bacterial synthetic biology using chromoproteins and designer antisense RNAs. As a bonus, practical material is provided for students of the annual international Genetically Engineered Machine (iGEM) competition. The manual is based upon a highly successful course at Sweden's Uppsala University and is coauthored by one of the pioneers of synthetic biology and two bioengineering postgraduate students. An inspiring foreword is written by another pioneer in the field, Harvard's George Church: "Synthetic biology is to early recombinant DNA as a genome is to a gene. Is there anything that SynBio will not impact? There was no doubt that the field of SynBio needed 'A Lab Manual' such as the one that you now hold in your hands." The ebook version of this title will be available in April 2014 | |
| 650 | 7 | |a SCIENCE / Chemistry / Industrial & Technical |2 bisacsh | |
| 650 | 7 | |a TECHNOLOGY & ENGINEERING / Chemical & Biochemical |2 bisacsh | |
| 650 | 7 | |a Synthetic biology |2 fast | |
| 650 | 4 | |a Chemie | |
| 650 | 4 | |a Synthetic biology |v Laboratory manuals | |
| 650 | 0 | 7 | |a Synthetische Biologie |0 (DE-588)7684405-5 |2 gnd |9 rswk-swf |
| 689 | 0 | 0 | |a Synthetische Biologie |0 (DE-588)7684405-5 |D s |
| 689 | 0 | |8 1\p |5 DE-604 | |
| 700 | 1 | |a Gullberg, Erik |e Sonstige |4 oth | |
| 700 | 1 | |a Forster, Anthony C. |e Sonstige |4 oth | |
| 856 | 4 | 0 | |u http://search.ebscohost.com/login.aspx?direct=true&scope=site&db=nlebk&db=nlabk&AN=779667 |x Aggregator |3 Volltext |
| 912 | |a ZDB-4-EBA | ||
| 999 | |a oai:aleph.bib-bvb.de:BVB01-028452023 | ||
| 883 | 1 | |8 1\p |a cgwrk |d 20201028 |q DE-101 |u https://d-nb.info/provenance/plan#cgwrk | |
| 966 | e | |u http://search.ebscohost.com/login.aspx?direct=true&scope=site&db=nlebk&db=nlabk&AN=779667 |l FAW01 |p ZDB-4-EBA |q FAW_PDA_EBA |x Aggregator |3 Volltext | |
| 966 | e | |u http://search.ebscohost.com/login.aspx?direct=true&scope=site&db=nlebk&db=nlabk&AN=779667 |l FAW02 |p ZDB-4-EBA |q FAW_PDA_EBA |x Aggregator |3 Volltext | |
Record in the Search Index
| _version_ | 1804175381402484736 |
|---|---|
| any_adam_object | |
| author | Liljeruhm, Josefine |
| author_facet | Liljeruhm, Josefine |
| author_role | aut |
| author_sort | Liljeruhm, Josefine |
| author_variant | j l jl |
| building | Verbundindex |
| bvnumber | BV043027369 |
| collection | ZDB-4-EBA |
| contents | 1. Introduction. What is synthetic biology, exactly? The iGEM outbreak. A synthetic biology lab manual -- 2. Genes, chromoproteins and antisense RNAs. E. coli DNA: Chromosomes, plasmids and copy number. Coupling of transcription and translation in bacteria. Promoter and terminator for transcription. Ribosome binding site (RBS). Codon bias. Chromoproteins. Small regulatory RNAs (sRNAs) -- 3. Lab rooms and equipment. The physical lab spaces. Equipment -- 4. Safety is priority #1. Fires. Chemicals. Biological safety and disposal. Dangerous equipment -- 5. Lab course projects. Time and resources. Project overview and learning objectives. The lab notebook. Lab section 1. Preparation of chemical solutions and agar plates. Lab section 2. Coloring bacteria by adding a promoter to a chromoprotein gene. Lab section 3: Rational engineering of chromoprotein expression level. Lab section 4. Other experiments. The "dreaded" exam -- 6. Protocols. Introduction. Protocol 1. Preparation of solutions and agar plates. Protocol 2. Overnight cultures with antibiotics, and glycerol stocks. Protocol 3. BioBrick 3A assembly and gel analysis. Protocol 4. Agarose gel electrophoresis. Protocol 5. Preparation of competent E. coli cells using CaCl2. Protocol 6. Transformation of CaCl2-competent E. coli cells. Protocol 7. Bacterial re-streaking techniques. Protocol 8. Lysis of E. coli cells with lysozyme. Protocol 9. Polymerase chain reaction (PCR). Protocol 10. Inverse PCR mutagenesis. Protocol 11. Colony PCR. Protocol 12. Gibson assembly -- 7. Advanced methods. Flow cytometry and cell sorting. Recombination in plasmids and the chromosome. Electrocompetent cells -- 8. The International Genetically Engineered Machine (iGEM) Competition. How to start an iGEM team. Uppsala iGEM 2011 -- Show color with color. Uppsala iGEM 2012 -- Resistance is futile. Uppsala iGEM 2013 -- Lactonutritious -- it's delicious -- 9. Appendices Synthetic Biology: A Lab Manual is the first manual for laboratory work in the new and rapidly expanding field of synthetic biology. Aimed at non-specialists, it details protocols central to synthetic biology in both education and research. In addition, it provides all the information that teachers and students from high schools and tertiary institutions need for a colorful lab course in bacterial synthetic biology using chromoproteins and designer antisense RNAs. As a bonus, practical material is provided for students of the annual international Genetically Engineered Machine (iGEM) competition. The manual is based upon a highly successful course at Sweden's Uppsala University and is coauthored by one of the pioneers of synthetic biology and two bioengineering postgraduate students. An inspiring foreword is written by another pioneer in the field, Harvard's George Church: "Synthetic biology is to early recombinant DNA as a genome is to a gene. Is there anything that SynBio will not impact? There was no doubt that the field of SynBio needed 'A Lab Manual' such as the one that you now hold in your hands." The ebook version of this title will be available in April 2014 |
| ctrlnum | (OCoLC)880354187 (DE-599)BVBBV043027369 |
| dewey-full | 660.6 |
| dewey-hundreds | 600 - Technology (Applied sciences) |
| dewey-ones | 660 - Chemical engineering |
| dewey-raw | 660.6 |
| dewey-search | 660.6 |
| dewey-sort | 3660.6 |
| dewey-tens | 660 - Chemical engineering |
| discipline | Chemie / Pharmazie |
| format | Electronic eBook |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>05196nmm a2200493zc 4500</leader><controlfield tag="001">BV043027369</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">00000000000000.0</controlfield><controlfield tag="007">cr|uuu---uuuuu</controlfield><controlfield tag="008">151120s2014 |||| o||u| ||||||eng d</controlfield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">9789814579551</subfield><subfield code="9">978-981-4579-55-1</subfield></datafield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">9814579556</subfield><subfield code="9">981-4579-55-6</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)880354187</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)BVBBV043027369</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rda</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-1046</subfield><subfield code="a">DE-1047</subfield></datafield><datafield tag="082" ind1="0" ind2=" "><subfield code="a">660.6</subfield><subfield code="2">23</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Liljeruhm, Josefine</subfield><subfield code="e">Verfasser</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Synthetic biology</subfield><subfield code="b">a lab manual</subfield><subfield code="c">Josefine Liljeruhm, Erik Gullberg, Anthony C. Forster, Uppsala University, Sweden</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">Hackensack, NJ</subfield><subfield code="b">World Scientific Publishing Company</subfield><subfield code="c">2014</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">1 online resource</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="500" ind1=" " ind2=" "><subfield code="a">Online resource; title from PDF title page (Ebsco; viewed on May 21, 2014)</subfield></datafield><datafield tag="505" ind1="8" ind2=" "><subfield code="a">1. Introduction. What is synthetic biology, exactly? The iGEM outbreak. A synthetic biology lab manual -- 2. Genes, chromoproteins and antisense RNAs. E. coli DNA: Chromosomes, plasmids and copy number. Coupling of transcription and translation in bacteria. Promoter and terminator for transcription. Ribosome binding site (RBS). Codon bias. Chromoproteins. Small regulatory RNAs (sRNAs) -- 3. Lab rooms and equipment. The physical lab spaces. Equipment -- 4. Safety is priority #1. Fires. Chemicals. Biological safety and disposal. Dangerous equipment -- 5. Lab course projects. Time and resources. Project overview and learning objectives. The lab notebook. Lab section 1. Preparation of chemical solutions and agar plates. Lab section 2. Coloring bacteria by adding a promoter to a chromoprotein gene. Lab section 3: Rational engineering of chromoprotein expression level. Lab section 4. Other experiments. The "dreaded" exam -- 6. Protocols. Introduction. Protocol 1. Preparation of solutions and agar plates. Protocol 2. Overnight cultures with antibiotics, and glycerol stocks. Protocol 3. BioBrick 3A assembly and gel analysis. Protocol 4. Agarose gel electrophoresis. Protocol 5. Preparation of competent E. coli cells using CaCl2. Protocol 6. Transformation of CaCl2-competent E. coli cells. Protocol 7. Bacterial re-streaking techniques. Protocol 8. Lysis of E. coli cells with lysozyme. Protocol 9. Polymerase chain reaction (PCR). Protocol 10. Inverse PCR mutagenesis. Protocol 11. Colony PCR. Protocol 12. Gibson assembly -- 7. Advanced methods. Flow cytometry and cell sorting. Recombination in plasmids and the chromosome. Electrocompetent cells -- 8. The International Genetically Engineered Machine (iGEM) Competition. How to start an iGEM team. Uppsala iGEM 2011 -- Show color with color. Uppsala iGEM 2012 -- Resistance is futile. Uppsala iGEM 2013 -- Lactonutritious -- it's delicious -- 9. Appendices</subfield></datafield><datafield tag="505" ind1="8" ind2=" "><subfield code="a">Synthetic Biology: A Lab Manual is the first manual for laboratory work in the new and rapidly expanding field of synthetic biology. Aimed at non-specialists, it details protocols central to synthetic biology in both education and research. In addition, it provides all the information that teachers and students from high schools and tertiary institutions need for a colorful lab course in bacterial synthetic biology using chromoproteins and designer antisense RNAs. As a bonus, practical material is provided for students of the annual international Genetically Engineered Machine (iGEM) competition. The manual is based upon a highly successful course at Sweden's Uppsala University and is coauthored by one of the pioneers of synthetic biology and two bioengineering postgraduate students. An inspiring foreword is written by another pioneer in the field, Harvard's George Church: "Synthetic biology is to early recombinant DNA as a genome is to a gene. Is there anything that SynBio will not impact? There was no doubt that the field of SynBio needed 'A Lab Manual' such as the one that you now hold in your hands." The ebook version of this title will be available in April 2014</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">SCIENCE / Chemistry / Industrial & Technical</subfield><subfield code="2">bisacsh</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">TECHNOLOGY & ENGINEERING / Chemical & Biochemical</subfield><subfield code="2">bisacsh</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Synthetic biology</subfield><subfield code="2">fast</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Chemie</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Synthetic biology</subfield><subfield code="v">Laboratory manuals</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Synthetische Biologie</subfield><subfield code="0">(DE-588)7684405-5</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="689" ind1="0" ind2="0"><subfield code="a">Synthetische Biologie</subfield><subfield code="0">(DE-588)7684405-5</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2=" "><subfield code="8">1\p</subfield><subfield code="5">DE-604</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Gullberg, Erik</subfield><subfield code="e">Sonstige</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Forster, Anthony C.</subfield><subfield code="e">Sonstige</subfield><subfield code="4">oth</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">http://search.ebscohost.com/login.aspx?direct=true&scope=site&db=nlebk&db=nlabk&AN=779667</subfield><subfield code="x">Aggregator</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-4-EBA</subfield></datafield><datafield tag="999" ind1=" " ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-028452023</subfield></datafield><datafield tag="883" ind1="1" ind2=" "><subfield code="8">1\p</subfield><subfield code="a">cgwrk</subfield><subfield code="d">20201028</subfield><subfield code="q">DE-101</subfield><subfield code="u">https://d-nb.info/provenance/plan#cgwrk</subfield></datafield><datafield tag="966" ind1="e" ind2=" "><subfield code="u">http://search.ebscohost.com/login.aspx?direct=true&scope=site&db=nlebk&db=nlabk&AN=779667</subfield><subfield code="l">FAW01</subfield><subfield code="p">ZDB-4-EBA</subfield><subfield code="q">FAW_PDA_EBA</subfield><subfield code="x">Aggregator</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="966" ind1="e" ind2=" "><subfield code="u">http://search.ebscohost.com/login.aspx?direct=true&scope=site&db=nlebk&db=nlabk&AN=779667</subfield><subfield code="l">FAW02</subfield><subfield code="p">ZDB-4-EBA</subfield><subfield code="q">FAW_PDA_EBA</subfield><subfield code="x">Aggregator</subfield><subfield code="3">Volltext</subfield></datafield></record></collection> |
| id | DE-604.BV043027369 |
| illustrated | Not Illustrated |
| indexdate | 2024-07-10T07:15:23Z |
| institution | BVB |
| isbn | 9789814579551 9814579556 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-028452023 |
| oclc_num | 880354187 |
| open_access_boolean | |
| owner | DE-1046 DE-1047 |
| owner_facet | DE-1046 DE-1047 |
| physical | 1 online resource |
| psigel | ZDB-4-EBA ZDB-4-EBA FAW_PDA_EBA |
| publishDate | 2014 |
| publishDateSearch | 2014 |
| publishDateSort | 2014 |
| publisher | World Scientific Publishing Company |
| record_format | marc |
| spelling | Liljeruhm, Josefine Verfasser aut Synthetic biology a lab manual Josefine Liljeruhm, Erik Gullberg, Anthony C. Forster, Uppsala University, Sweden Hackensack, NJ World Scientific Publishing Company 2014 1 online resource txt rdacontent c rdamedia cr rdacarrier Online resource; title from PDF title page (Ebsco; viewed on May 21, 2014) 1. Introduction. What is synthetic biology, exactly? The iGEM outbreak. A synthetic biology lab manual -- 2. Genes, chromoproteins and antisense RNAs. E. coli DNA: Chromosomes, plasmids and copy number. Coupling of transcription and translation in bacteria. Promoter and terminator for transcription. Ribosome binding site (RBS). Codon bias. Chromoproteins. Small regulatory RNAs (sRNAs) -- 3. Lab rooms and equipment. The physical lab spaces. Equipment -- 4. Safety is priority #1. Fires. Chemicals. Biological safety and disposal. Dangerous equipment -- 5. Lab course projects. Time and resources. Project overview and learning objectives. The lab notebook. Lab section 1. Preparation of chemical solutions and agar plates. Lab section 2. Coloring bacteria by adding a promoter to a chromoprotein gene. Lab section 3: Rational engineering of chromoprotein expression level. Lab section 4. Other experiments. The "dreaded" exam -- 6. Protocols. Introduction. Protocol 1. Preparation of solutions and agar plates. Protocol 2. Overnight cultures with antibiotics, and glycerol stocks. Protocol 3. BioBrick 3A assembly and gel analysis. Protocol 4. Agarose gel electrophoresis. Protocol 5. Preparation of competent E. coli cells using CaCl2. Protocol 6. Transformation of CaCl2-competent E. coli cells. Protocol 7. Bacterial re-streaking techniques. Protocol 8. Lysis of E. coli cells with lysozyme. Protocol 9. Polymerase chain reaction (PCR). Protocol 10. Inverse PCR mutagenesis. Protocol 11. Colony PCR. Protocol 12. Gibson assembly -- 7. Advanced methods. Flow cytometry and cell sorting. Recombination in plasmids and the chromosome. Electrocompetent cells -- 8. The International Genetically Engineered Machine (iGEM) Competition. How to start an iGEM team. Uppsala iGEM 2011 -- Show color with color. Uppsala iGEM 2012 -- Resistance is futile. Uppsala iGEM 2013 -- Lactonutritious -- it's delicious -- 9. Appendices Synthetic Biology: A Lab Manual is the first manual for laboratory work in the new and rapidly expanding field of synthetic biology. Aimed at non-specialists, it details protocols central to synthetic biology in both education and research. In addition, it provides all the information that teachers and students from high schools and tertiary institutions need for a colorful lab course in bacterial synthetic biology using chromoproteins and designer antisense RNAs. As a bonus, practical material is provided for students of the annual international Genetically Engineered Machine (iGEM) competition. The manual is based upon a highly successful course at Sweden's Uppsala University and is coauthored by one of the pioneers of synthetic biology and two bioengineering postgraduate students. An inspiring foreword is written by another pioneer in the field, Harvard's George Church: "Synthetic biology is to early recombinant DNA as a genome is to a gene. Is there anything that SynBio will not impact? There was no doubt that the field of SynBio needed 'A Lab Manual' such as the one that you now hold in your hands." The ebook version of this title will be available in April 2014 SCIENCE / Chemistry / Industrial & Technical bisacsh TECHNOLOGY & ENGINEERING / Chemical & Biochemical bisacsh Synthetic biology fast Chemie Synthetic biology Laboratory manuals Synthetische Biologie (DE-588)7684405-5 gnd rswk-swf Synthetische Biologie (DE-588)7684405-5 s 1\p DE-604 Gullberg, Erik Sonstige oth Forster, Anthony C. Sonstige oth http://search.ebscohost.com/login.aspx?direct=true&scope=site&db=nlebk&db=nlabk&AN=779667 Aggregator Volltext 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Liljeruhm, Josefine Synthetic biology a lab manual 1. Introduction. What is synthetic biology, exactly? The iGEM outbreak. A synthetic biology lab manual -- 2. Genes, chromoproteins and antisense RNAs. E. coli DNA: Chromosomes, plasmids and copy number. Coupling of transcription and translation in bacteria. Promoter and terminator for transcription. Ribosome binding site (RBS). Codon bias. Chromoproteins. Small regulatory RNAs (sRNAs) -- 3. Lab rooms and equipment. The physical lab spaces. Equipment -- 4. Safety is priority #1. Fires. Chemicals. Biological safety and disposal. Dangerous equipment -- 5. Lab course projects. Time and resources. Project overview and learning objectives. The lab notebook. Lab section 1. Preparation of chemical solutions and agar plates. Lab section 2. Coloring bacteria by adding a promoter to a chromoprotein gene. Lab section 3: Rational engineering of chromoprotein expression level. Lab section 4. Other experiments. The "dreaded" exam -- 6. Protocols. Introduction. Protocol 1. Preparation of solutions and agar plates. Protocol 2. Overnight cultures with antibiotics, and glycerol stocks. Protocol 3. BioBrick 3A assembly and gel analysis. Protocol 4. Agarose gel electrophoresis. Protocol 5. Preparation of competent E. coli cells using CaCl2. Protocol 6. Transformation of CaCl2-competent E. coli cells. Protocol 7. Bacterial re-streaking techniques. Protocol 8. Lysis of E. coli cells with lysozyme. Protocol 9. Polymerase chain reaction (PCR). Protocol 10. Inverse PCR mutagenesis. Protocol 11. Colony PCR. Protocol 12. Gibson assembly -- 7. Advanced methods. Flow cytometry and cell sorting. Recombination in plasmids and the chromosome. Electrocompetent cells -- 8. The International Genetically Engineered Machine (iGEM) Competition. How to start an iGEM team. Uppsala iGEM 2011 -- Show color with color. Uppsala iGEM 2012 -- Resistance is futile. Uppsala iGEM 2013 -- Lactonutritious -- it's delicious -- 9. Appendices Synthetic Biology: A Lab Manual is the first manual for laboratory work in the new and rapidly expanding field of synthetic biology. Aimed at non-specialists, it details protocols central to synthetic biology in both education and research. In addition, it provides all the information that teachers and students from high schools and tertiary institutions need for a colorful lab course in bacterial synthetic biology using chromoproteins and designer antisense RNAs. As a bonus, practical material is provided for students of the annual international Genetically Engineered Machine (iGEM) competition. The manual is based upon a highly successful course at Sweden's Uppsala University and is coauthored by one of the pioneers of synthetic biology and two bioengineering postgraduate students. An inspiring foreword is written by another pioneer in the field, Harvard's George Church: "Synthetic biology is to early recombinant DNA as a genome is to a gene. Is there anything that SynBio will not impact? There was no doubt that the field of SynBio needed 'A Lab Manual' such as the one that you now hold in your hands." The ebook version of this title will be available in April 2014 SCIENCE / Chemistry / Industrial & Technical bisacsh TECHNOLOGY & ENGINEERING / Chemical & Biochemical bisacsh Synthetic biology fast Chemie Synthetic biology Laboratory manuals Synthetische Biologie (DE-588)7684405-5 gnd |
| subject_GND | (DE-588)7684405-5 |
| title | Synthetic biology a lab manual |
| title_auth | Synthetic biology a lab manual |
| title_exact_search | Synthetic biology a lab manual |
| title_full | Synthetic biology a lab manual Josefine Liljeruhm, Erik Gullberg, Anthony C. Forster, Uppsala University, Sweden |
| title_fullStr | Synthetic biology a lab manual Josefine Liljeruhm, Erik Gullberg, Anthony C. Forster, Uppsala University, Sweden |
| title_full_unstemmed | Synthetic biology a lab manual Josefine Liljeruhm, Erik Gullberg, Anthony C. Forster, Uppsala University, Sweden |
| title_short | Synthetic biology |
| title_sort | synthetic biology a lab manual |
| title_sub | a lab manual |
| topic | SCIENCE / Chemistry / Industrial & Technical bisacsh TECHNOLOGY & ENGINEERING / Chemical & Biochemical bisacsh Synthetic biology fast Chemie Synthetic biology Laboratory manuals Synthetische Biologie (DE-588)7684405-5 gnd |
| topic_facet | SCIENCE / Chemistry / Industrial & Technical TECHNOLOGY & ENGINEERING / Chemical & Biochemical Synthetic biology Chemie Synthetic biology Laboratory manuals Synthetische Biologie |
| url | http://search.ebscohost.com/login.aspx?direct=true&scope=site&db=nlebk&db=nlabk&AN=779667 |
| work_keys_str_mv | AT liljeruhmjosefine syntheticbiologyalabmanual AT gullbergerik syntheticbiologyalabmanual AT forsteranthonyc syntheticbiologyalabmanual |