Detection of blotted proteins: methods and protocols
This detailed volume provides methods and techniques for detection after blotting. Chapters guide readers through a number of variations on the theme of protein transfer to solid support followed by detection, presenting adaptations of traditional techniques, and original methods of protein blotting...
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| Weitere Verfasser: | , |
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| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
New York
Humana Press
[2015]
|
| Schriftenreihe: | Methods in molecular biology
1314 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Zusammenfassung: | This detailed volume provides methods and techniques for detection after blotting. Chapters guide readers through a number of variations on the theme of protein transfer to solid support followed by detection, presenting adaptations of traditional techniques, and original methods of protein blotting. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, Detection of Blotted Proteins: Methods and Protocols presents numerous techniques based on the Western blot, providing detailed, readily reproducible methods, tips, and alternatives directly and easily transferable to the laboratory setting |
| Beschreibung: | xiv, 386 Seiten Illustrationen, Diagramme |
| ISBN: | 1493927175 9781493927173 |
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| 520 | 1 | |a This detailed volume provides methods and techniques for detection after blotting. Chapters guide readers through a number of variations on the theme of protein transfer to solid support followed by detection, presenting adaptations of traditional techniques, and original methods of protein blotting. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, Detection of Blotted Proteins: Methods and Protocols presents numerous techniques based on the Western blot, providing detailed, readily reproducible methods, tips, and alternatives directly and easily transferable to the laboratory setting | |
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Datensatz im Suchindex
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| adam_text | Titel: Detection of blotted proteins
Autor: Kurien, Biji T
Jahr: 2015
Contents
Preface.......................................................... v
Contributors.......................................................... xi
1 Western Blotting: Origin and Ascent of the Species..................... 1
W. Neal Burnette
2 Methods to Concentrate Proteins for Protein Isolation, Proteomic,
and Peptidomic Evaluation....................................... 5
J.P. Dean Goldring
3 Measuring Protein Concentration on Nitrocellulose
and After the Electrophoretic Transfer of Protein to Nitrocellulose......... 19
J.P. Dean Goldring
4 Detection of Blotted Proteins: Not All Blockers Are Created Equal......... 27
Vishal Kothari and Surest) T. Mathews
5 Protein Stains to Detect Antigen on Membranes....................... 33
Anil Dsouza and R. Hal Scofield
6 Fluorescent Labeling of Proteins and Its Application to SDS-PAGE
and Western Blotting........................................... 41
F. Javier Alba, Salvador Bartolome, Antonio Bermudez,
and Joan-Ramon Daban
7 Rapid, Antibody-Free Detection of Recombinant Proteins on Blots
Using Enzyme Fragment Complementation.......................... 51
Neil W. Charter, Joe Horecka, Chin-Tee Loh, Albert Doan,
Tom Wehrman, and Keith R. Olson
8 Use of Nonradioactive Detection Method for North-
and South-Western Blot......................................... 63
Claudia Franke, Daniel GrUfe, Holger Bartsch, and Michael P. Bachmann
9 Immunoblotting Using Radiolabeled Reagents for Detection............. 73
Holger Bartsch, Claudia Franke, and Michael P. Bachmann
10 Immunoblotting of Antigens: Whole, Strip, and New-Line Nitrocellulose
Membrane Immunoblotting Using the Chemiluminescence Technique...... 79
TaserDorri
11 Detection of Protein Carbonyls by Means of Biotin
Hydrazide^Streptavidin Affinity Methods............................ 95
Kenneth Hensley
12 Direct Immunodetection of Antigens Within the Precast
Polyacrylamide Gel............................................. 101
Surbhi Desai, Boguslawa R. Dworecki, and Marie C. Nlend
vii
VIII
Contents
13 Quantitative Analysis of Signal Transduction with In-Cell Western
Immunofluorescence Assays.............................
Vince Boveia and Amy Schutz-Geschwender
115
14 Ultrasensitive Protein Detection on Dot Blots and Western Blots
with Semiconducting Polymer Dots......................
Fangmao Te, Polina B. Smith, and Daniel T. Chin
131
15 Co-detection of Target and Total Protein by CyDye Labeling
and Fluorescent ECL Plex Immunoblotting in a Standard
Proteomics Workflow..............................
139
Caitriona Scaife, Ciara A. McManus, Pamela M. Donoghue,
and Michael J. Dunn
16 Using Biotinylated Proteins to Demonstrate Immunodetection
of Antigens via Western Blotting, Dot Blots, and Immunohistochemistry .... 151
Thomas Millar, Ronald Knighton, and Jo-Anne Chuck
17 Calcium Binding by Ro 60 Multiple Antigenic Peptides
on PVDF Membrane............................................ 165
Biji T. Kurien and Michael P. Bachmann
18 Sequential Use of Immunoblots for Characterization
of Autoantibody Specificities...................................... 173
Holger Bartsch and Michael P. Bachmann
19 Nanogold Immunodetection Detection Systems for the Identification
of Autoantigens by Western Blotting................................ 179
Jacen S. Moore and R. Hal Scofield
20 Application of Intermittent Microwave Irradiation
to Western Blot Analysis......................................... 185
Tu-Ting Liu and Shinya Toyokuni
21 Visualization of Unstained Protein Bands on PVDF.................... 191
Jun Park, Masaharu Mabuchi, and Ajay Sharma
22 Multiplexed Fluorescent Immunodetection Using Low
Autofluorescence Immobilon®-FL Membrane......................... 195
Jun Park, Masaharu Mabuchi, and Ajay Sharma
23 Cold Microwave-Enabled Protein Detection and Quantification........... 207
Niels Griitzner, Romy M. Heilmann, Aaron G. Smith, Carol B. Johnson,
Stanislav Vitha, Jorg M. Steiner, and Andreas K. Holzenburg
24 TLC-Blot (Far-Eastern Blot) and Its Application to Functional Lipidomics..... 219
Takao Taki
25 Analysis of Electroblotted Proteins by Mass Spectrometry................ 243
Jose L. Luque-Garcia and Thomas A. Neubert
26 On-Membrane Renaturation of Recombinant Ro60 Autoantigen
by Calcium Ions............................................... 255
Biji T. Kurien and Michael P. Bachmann
27 Phosphoprotein Detection on Protein Electroblot
Using a Phosphate-Specific Fluorophore............................. 263
Lee Broderick Bockus and R. Hal Scofield
Contents ix
28 Purification of Tryptic Digests on Polyvinylidene Difluoride Membrane..... 273
Biji T. Kurien and R. Hal Scofield
29 Detection of Blotted Proteins on Nitrocellulose/PVDF
Membranes by Alta............................................. 279
Jayanta K. Pal, Shilpa J. Rao, and Dhanashri J. Godbole
30 Nonstripping Rainbow and Multiple Antigen Detection (MAD)
Western Blotting............................................... 287
Stan (Stanislaw) Krajewski, Michelle M. Tsukamoto, Xianshu Huang,
and Sebastian B. Krajewski
31 Supported Molecular Matrix Electrophoresis.......................... 303
Yu-ki Matsuno and Akihiko Kameyama
32 Parafilm-M®, An Available Cost-Effective Alternative
for Immuno-blot Pouches........................................ 313
Syed M.S. Quadri
33 Succinylation-Alcian Blue Staining of Mucins on Polyvinylidene
Difluoride Membranes.......................................... 325
Akihiko Kameyama, Weijie Dong, and Yu-ki Matsuno
34 Comparison of Chemiluminescence vs. Infrared Techniques
for Detection of Fetuin-A in Saliva................................. 333
Suresh T. Mathews, Emily Graff, Robert L. Judd, and Vishal Kothari
35 A Novel Methodology for Stripping and Reprobing of Western Blots
Originally Developed with Colorimetric Substrate TMB................. 349
Parmita Kar, Saurabh Kumar Agnihotri, Archana Sharma,
Rekha Sachan, Madan Lai Bhatt, and Monika Sachdev
36 Other Notable Methods of Membrane Protein Detection: A Brief Review. . . . 357
Biji T. Kurien and R. Hal Scofield
37 Nitrocellulose Membrane: The New Canvas.......................... 371
Jasmin R. Kurien and Bianca A. Kurien
38 Invisible Ink Marking in ECL Membrane Assays....................... 375
Biji T. Kurien
Index............................................................... 383
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| spelling | Detection of blotted proteins methods and protocols edited by Biji T. Kurien, R. Hal Scofield New York Humana Press [2015] © 2015 xiv, 386 Seiten Illustrationen, Diagramme txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 1314 Springer protocols This detailed volume provides methods and techniques for detection after blotting. Chapters guide readers through a number of variations on the theme of protein transfer to solid support followed by detection, presenting adaptations of traditional techniques, and original methods of protein blotting. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, Detection of Blotted Proteins: Methods and Protocols presents numerous techniques based on the Western blot, providing detailed, readily reproducible methods, tips, and alternatives directly and easily transferable to the laboratory setting Proteine (DE-588)4076388-2 gnd rswk-swf Blotting (DE-588)4412077-1 gnd rswk-swf Untersuchungsmethode (DE-588)4324907-3 gnd rswk-swf Nachweis (DE-588)4115334-0 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Proteine (DE-588)4076388-2 s Blotting (DE-588)4412077-1 s Nachweis (DE-588)4115334-0 s Untersuchungsmethode (DE-588)4324907-3 s DE-604 Kurien, Biji T. ca. 20./21. Jh. (DE-588)1079693750 edt Scofield, R. Hal ca. 20./21. Jh. (DE-588)1079693904 edt Erscheint auch als Online-Ausgabe 978-1-4939-2718-0 Methods in molecular biology 1314 (DE-604)BV035362695 1314 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=028446987&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Detection of blotted proteins methods and protocols Methods in molecular biology Proteine (DE-588)4076388-2 gnd Blotting (DE-588)4412077-1 gnd Untersuchungsmethode (DE-588)4324907-3 gnd Nachweis (DE-588)4115334-0 gnd |
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| title | Detection of blotted proteins methods and protocols |
| title_auth | Detection of blotted proteins methods and protocols |
| title_exact_search | Detection of blotted proteins methods and protocols |
| title_full | Detection of blotted proteins methods and protocols edited by Biji T. Kurien, R. Hal Scofield |
| title_fullStr | Detection of blotted proteins methods and protocols edited by Biji T. Kurien, R. Hal Scofield |
| title_full_unstemmed | Detection of blotted proteins methods and protocols edited by Biji T. Kurien, R. Hal Scofield |
| title_short | Detection of blotted proteins |
| title_sort | detection of blotted proteins methods and protocols |
| title_sub | methods and protocols |
| topic | Proteine (DE-588)4076388-2 gnd Blotting (DE-588)4412077-1 gnd Untersuchungsmethode (DE-588)4324907-3 gnd Nachweis (DE-588)4115334-0 gnd |
| topic_facet | Proteine Blotting Untersuchungsmethode Nachweis Aufsatzsammlung |
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