Sorting and recycling endosomes:
Saved in:
| Format: | Book |
|---|---|
| Language: | English |
| Published: |
Amsterdam [u.a.]
Elsevier, Academic Press
2015
|
| Edition: | 1. ed. |
| Series: | Methods in cell biology
130 |
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | XIX, 396 S. Ill., graph. Darst., [16] Bl. |
| ISBN: | 9780128028292 |
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MARC
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| 245 | 1 | 0 | |a Sorting and recycling endosomes |c ed. by Wei Guo |
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Record in the Search Index
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|---|---|
| adam_text | Contents
Contributors.......................................................xiii
Preface.............................................................xix
CHAPTER 1 Dynamic Imaging of the Recycling Endosomal
Network in Macrophages.................................1
Adam A. Wall, Nicholas D. Condon, Jeremy C. Yeo,
Nicholas A. Hamilton, Jennifer L. Stow
Introduction...........................................2
1. Methods................................................4
2. Expenmental Strategies.................................5
Summary...............................................16
References............................................17
CHAPTER 2 Analyzing the Functions of Rab 11-effector
Proteins During Cell Division.........................19
Rytis Prekeris
Introduction..........................................20
1. Methods for Analyzing Proteins Regulating Cytokinesis.22
2. Time-Lapse Analysis of Endosome and Actin Dynamics
during Cytokinesis....................................27
Summary...............................................33
Acknowledgments.......................................33
References......................................... 33
CHAPTER 3 Expression and Localization of Exocytic and
Recycling Rabs from Magnaporthe Oryzae in
Mammalian Cells.......................................35
Yaoyao Qi, M. Caleb Marlin, Zbimin Liang,
Dongmei Zhang, iie Zhou, Zonghua Wang, Guodong Lu,
Guangpu Li
Introduction..........................................36
1. Materials and Equipment...............................37
2. Methods...............................................38
Summary.............................................. 42
Acknowledgments.......................................44
References............................................44
vi Contents
CHAPTER 4 Methods to Analyze Novel Liaisons between
Endosomes and Centrosomes.................................47
Hui-Fang Hung, Heidi Hehnly, Stephen Doxsey
Introduction: The Relationship between the Recycling
Endosome and Centrosome...................................48
1. Live Cell Imaging of REs throughout the Cell Cycle.....50
2. RE’s Centrosome Localization by Electron Microscopy...51
3. Centrosome Isolation and Identification of RE Components.53
Acknowledgments....................................... 56
References.............................................56
CHAPTER 5 Biochemical Analysis of Rabin8, the Guanine
Nucleotide Exchange Factor for Rab8....................59
Bin Wu, Juanfei Wang, Yuting Zhao, Wei Guo
Introduction...........................................60
1. Method.................................................61
Acknowledgments........................................66
References.............................................67
CHAPTER 6 Simple In Vitro Assay of Arf GAPs and Preparation
of Arf Proteins as Substrates..........................69
Pei-Wen Chen, Xiaoying Jian, Ruibai Luo,
Paul A. Randazzo
Introduction........................................ 70
1. Methods............................................... 71
Summary........................................ 78
Acknowledgment.........................................78
References..................».............................78
CHAPTER 7 An ACAP1 Coat Complex Acting in Endocytic
Recycling............................................ 81
Jian Li, Victor W. Hsu
Introduction...........................................82
1. Materials..............................................83
2. Methods...............................................85
Discussion and Perspective.............................96
Acknowledgments........................................97
References.............................................97
Contents vii
CHAPTER 8 Biochemical Methods for Studying Kinetic
Regulation of Arf 1 Activation by Sec7...................101
Brian C. Richardson, J. Christopher Fromme
Introduction.............................................103
1. Materials...............................................104
2. Purification of Required Proteins.......................106
3. Liposomes...............................................115
4. Measuring GEF Activity..................................117
5. Identifying Protein—Arfl Interactions...................121
6. Supporting Protocol.....................................123
Acknowledgments..........................................125
References...............................................125
CHAPTER 9 Rab and Arf G Proteins in Endosomal Trafficking... 127
Dipannita Dutta, Julie G. Donaldson
Introduction.............................................128
1. Objectives and Rationale................................130
2. Materials and Instruments...............................131
3. Methods.................................................132
Summary..................................................137
References...............................................137
CHAPTER 10 Qualitative and Quantitative Analysis of
Endocytic Recycling......................................139
James B. Reineke, Shuwei Xie, Naava Naslavsky,
Steve Caplan
Introduction.............................................140
1. Materials...............................................142
2. Qualitative Assessment of Endocytic Recycling...........143
3. Quantitative Assessment of Endocytic Recycling..........149
4. Quantification of MHC I Antibody-Receptor Complex
Recycling Using Immunofluorescence Microscopy
and ImageJ...............................................152
Acknowledgments..........................................154
References...............................................154
viii Contents
CHAPTER 11 Methods for Analyzing the Role of
Phospholipase A2 Enzymes in Endosome
Membrane Tubule Formation...............................157
Danielle N. Kalkofen, Paul de Figueiredo, William J. Brown
Introduction............................................158
1. In Vitro Endosome Membrane Tubule Formation............160
2. In Vivo Endosome Membrane Tubule Formation............167
3. PLA2 Enzymes in Endosome Membrane Tubule Formation... 174
Acknowledgments.........................................178
References..............................................178
CHAPTER 12 In Vivo Analysis of Recycling Endosomes
in Caenorhabditis elega ns..............................181
Anbing Shi, Barth D. Grant
Introduction............................................182
1. Objectives and Rationale...............................183
2. Matenals and Instruments...............................184
3. Methods................................................184
Summary.................................................195
References............................................. 195
CHAPTER 13 Monitoring Receptor Trafficking Following
Retromer and WASH Deregulation..........................199
Douglas G. Osborne, Christine A. Phillips-Krawczak,
Daniel D. Biiladeau
Introduction............................................200
1. Analysis of Retrograde Trafficking; Objectives and
Rationale...............................................201
2. Experimental Strategy................................ 203
3. Methods................................................204
4. Experimental Analysis..................................206
5. Analysis of Integral Recycling: Objectives and Rationale.208
6. Experimental Strategy..................................208
7. Methods................................................209
8. Experimental Analysis..................................210
Summary.................................................212
References..............................................212
Contents
CHAPTER 14 Quantitative Analysis of Endosome Tubulation
and Microdomain Organization Mediated by
the WASH Complex.......................................................215
Emmanuel Derivery, Alexis Gautreau
Introduction............................................216
1. Imaging of Endosome Tubulation.........................218
2. Live Imaging of WASH Microdomains......................222
3. Quantitative 3D Analysis of WASH Domain Localization
in Fixed Cells..........................................225
Conclusion..............................................231
Acknowledgments.........................................232
References..............................................232
CHAPTER 15 Rab GTPases and Kinesin Motors in Endosomal
Trafficking.............................................235
Cédric Delevoye, Bruno Goud
Introduction............................................236
1. Screen for Rab GTPases—Kinesin Interactions............236
2. Spatiotemporal Distribution of Kinesins and Rab GTPases.... 237
3. Role of the Microtubule Cytoskeleton in the Dynamics
of Kinesin and Rab GTPases..............................239
4. Functional Assays......................................239
Conclusions.............................................245
References..............................................246
CHAPTER 16 Measuring Receptor Recycling in Polarized
MDCK Cells..............................................247
Luciana Gallo, Gerard Apodaca
Introduction............................................248
1. Objectives and Rationale...............................250
2. Materials and Instruments..............................253
3. Methods................................................257
Summary.................................................266
References..............................................267
x Contents
CHAPTER 17 Structural and Functional Analysis of
Endosomal Compartments in Epithelial Cells.......271
Andres E. Perez Bay, Ryan Schreiner,
Enrique Rodriguez-Boulan
Introduction...........................................272
1. Objectives and Rationale...............................275
2. Experimental Protocols.................................275
References.............................................285
CHAPTER 18 Analyzing the Role of AP-1B in Polarized
Sorting from Recycling Endosomes in Epithelial
Cells..................................................289
Heike Folsch
Introduction...........................................290
1. Objectives and Rationale...............................291
2. Materials and Instruments..............................292
3. Methods................................................293
Summary................................................303
Acknowledgments........................................303
References.............................................303
CHAPTER 19 Recycling of the Insulin-responsive Glucose
Transporter Glut4 Regulated by the Small
GTPase RalA and the Exocyst Complex..................307
Chao Nie, Xiao-Wei Chen
Introduction...........................................308
1. Objective and Rationale................................309
2. Materials and Instruments..............................310
3. Methods and Results....................................311
Summary................................................317
References.............................................317
CHAPTER 20 GPCR Sorting at Multivesicular Endosomes.............319
Michael Robert Dores, JoAnn Trejo
Introduction...........................................320
1. Objectives and Rationale...............................321
2. Materials and Instruments..............................322
3. Methods................................................323
Summary................................................330
References.............................................330
Contents
XI
CHAPTER 21 Visualizing and Quantitating Sequence-dependent
GPCR Recycling........................................ 333
Shanna L. Bowman, Amanda L. Soohoo,
Manojkumar A. Puthenveedu
Introduction...........................................334
1. Objectives and Rationale..............................335
2. Materials and Instruments.............................335
3. Methods............................................. 336
Summary................................................344
References.............................................344
CHAPTER 22 Methods to Study Endocytic Trafficking of the
EGF Receptor...........................................347
Itziar Pinilla-Macua, Alexander Sorkin
Introduction...........................................348
1. Objectives and Rationale..............................349
2. Materials.............................................350
3. Basic Techniques......................................351
4. Experimental Strategies...............................352
Summary................................................366
References.............................................366
Volumes in Series...................................................369
Index...............................................................383
|
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| series2 | Methods in cell biology |
| spelling | Sorting and recycling endosomes ed. by Wei Guo 1. ed. Amsterdam [u.a.] Elsevier, Academic Press 2015 XIX, 396 S. Ill., graph. Darst., [16] Bl. txt rdacontent n rdamedia nc rdacarrier Methods in cell biology 130 Molekularbiologische Methode (DE-588)7650063-9 gnd rswk-swf Endosom (DE-588)4570819-8 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Endosom (DE-588)4570819-8 s Molekularbiologische Methode (DE-588)7650063-9 s b DE-604 Guo, Wei Sonstige oth Methods in cell biology 130 (DE-604)BV002534878 130 Digitalisierung UB Regensburg - ADAM Catalogue Enrichment application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=028361575&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Sorting and recycling endosomes Methods in cell biology Molekularbiologische Methode (DE-588)7650063-9 gnd Endosom (DE-588)4570819-8 gnd |
| subject_GND | (DE-588)7650063-9 (DE-588)4570819-8 (DE-588)4143413-4 |
| title | Sorting and recycling endosomes |
| title_auth | Sorting and recycling endosomes |
| title_exact_search | Sorting and recycling endosomes |
| title_full | Sorting and recycling endosomes ed. by Wei Guo |
| title_fullStr | Sorting and recycling endosomes ed. by Wei Guo |
| title_full_unstemmed | Sorting and recycling endosomes ed. by Wei Guo |
| title_short | Sorting and recycling endosomes |
| title_sort | sorting and recycling endosomes |
| topic | Molekularbiologische Methode (DE-588)7650063-9 gnd Endosom (DE-588)4570819-8 gnd |
| topic_facet | Molekularbiologische Methode Endosom Aufsatzsammlung |
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