Bioanalytical chemistry:
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| Main Authors: | , , , |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
London
Imperial College Press
[2015]
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| Edition: | Second edition |
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | xx, 236 Seiten Illustrationen, Diagramme |
| ISBN: | 9781783266715 9781783266722 |
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Record in the Search Index
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| adam_text | Titel: Bioanalytical chemistry
Autor: Manz, Andreas
Jahr: 2015
CONTENTS Preface to the First Edition ix Preface to the Second Edition xi List of Abbreviations xiii Chapter 1 Biomolecules 1 1.1 Amino Acids, Peptides and Proteins 1 1.1.1 Amino Acids 2 1.1.2 Peptides and Proteins 7 1.2 Nucleic Acids 14 1.2.1 The Structure of Nucleic Acids 15 1.2.2 Synthesis of Proteins 20 1.3 Biomolecules in Analytical Chemistry 22 1.3.1 Classical Analytical Chemistry 22 1.3.2 Limitations of Classical Analytical Chemistry 23 1.3.3 Bioanalytical Chemistry 23 Summary 28 References 29 Chapter 2 Chromatography 31 2.1 The Principle of Chromatography 31 2.2 Basic Chromatographic Theory 33 2.3 Liquid Chromatography Techniques for Bioanalysis 36 2.3.1 Liquid Chromatography Instrumentation 37 2.3.2 Reversed-Phase Liquid Chromatography (RP-LC) 39 2.3.3 Hydrophilic Interaction Liquid Chromatography (HILIC) 41 2.3.4 Ion-Exchange Chromatography 43 2.3.5 Affinity Chromatography 47 2.3.6 Size Exclusion Chromatography (SEC) 49 Summary 53 References 53 v
vi Bioanalytical Chemistry, 2nd Edition Chapter 3 Electrophoresis 55 3.1 Principle and Theory of Electrophoresis 56 3.1.1 Electrophoretic Mobility 57 3.1.2 Joule Heating 58 3.1.3 Electroosmotic Flow (EOF) 58 3.1.4 Separation Efficiency and Resolution 62 3.2 Gel Electrophoresis (GE) 64 3.2.1 Instrumentation for Gel Electrophoresis 65 3.2.2 Modes of Gel Electrophoresis 70 3.2.3 Sodium-Dodecyl Sulfate — Polyacryl Amide Gel Electrophoresis (SDS-PAGE) 71 3.2.4 Isoelectric Focussing (IEF) 71 3.2.5 Two-Dimensional Gel Electrophoresis (2D-GE) 75 3.3 Capillary Electrophoresis (CE) 77 3.3.1 Capillary Electrophoresis Instrumentation 78 3.3.2 Capillary Zone Electrophoresis (CZE) 82 3.3.3 Capillary Isoelectric Focussing (CIEF) 83 3.3.4 Micellar Electrokinetic Chromatography (MEKC) 85 3.3.5 Capillary Gel Electrophoresis (CGE) 89 Summary 91 References 92 Chapter 4 Mass Spectrometry 93 4.1 The Principle of Mass Spectrometry 93 4.1.1 Ionisation 94 4.1.2 Mass Analyser 94 4.1.3 Detector 94 4.2 Matrix-Assisted Laser Desorption Ionisation — Time-of-Flight Mass Spectrometry (MALDI-TOF-MS) 95 4.2.1 Ionisation Principle 96 4.2.2 Mass Analysis in Time-of-Flight Analyser 96 4.2.3 Detection of Ions 100 4.2.4 Resolution 100 4.2.5 Sample Pre-treatment 101 4.2.6 Applications of MALDI 103 4.3 Electrospray Ionisation Mass Spectrometry (ESI-MS) 105 4.3.1 Ionisation Principle 106 4.3.2 ESI-Source and Interface 108 4.3.3 Quadrupole Analyser 109 4.3.4 Applications of ESI-MS 110 Summary 115 References 115
Contents vii Chapter 5 Optical Spectroscopy 117 5.1 Absorption of UV and Visible Light 118 5.1.1 Basic Principles of Absorption Measurements 118 5.2 Fundamentals of Fluorescence Spectroscopy 120 5.2.1 Introduction to Fluorescence 120 5.2.2 Instrumentation 122 5.2.3 Fluorescence Parameters 123 5.2.4 Signal Intensity 126 5.2.5 Fluorophores and Labelling Strategies 128 5.2.6 Fluorescence Imaging 136 Summary 140 References 141 Chapter 6 Molecular Recognition in Immunoassays, Biosensors, DNA-Arrays and Pyrosequencing 143 6.1 Bioassays 144 6.1.1 Antibodies 145 6.1.2 Antigens 147 6.1.3 Antibody-Antigen-Complex Formation 148 6.1.4 Assay Formats 149 6.1.5 Home Pregnancy Test 154 6.1.6 Enzyme Immunoassays (El and ELISA) 156 6.2 Biosensors 159 6.2.1 The Bioreceptor 160 6.2.2 The Transducer 162 6.2.3 Blood Glucose Sensor 163 6.3 DNA Binding Arrays 165 6.3.1 Principle of DNA-Arrays 166 6.3.2 Fabrication of DNA-Arrays 167 6.3.3 Development and Analysis of a DNA-Array 167 6.3.4 Sequencing of DNA with Arrays 168 6.3.5 Other Applications of DNA-Arrays 170 6.4 DNA Identification by Pyrosequencing 171 6.4.1 The Principle of Pyrosequencing 171 6.4.2 Sample Preparation and Instrumentation 174 6.4.3 Applications of Pyrosequencing 175 Summary 175 References 176
Bioanalytical Chemistry, 2nd Edition viii Chapter 7 Nucleic Acid Analysis 177 7.1 Extraction and Isolation of Nucleic Acids 178 7.1.1 CsCl Density Gradient Centrifugation 178 7.1.2 Total Cellular DNA Isolation 179 7.1.3 RNA Isolation — the Proteinase K Method 179 7.2 Nucleic Acid Amplification — the Polymerase Chain Reaction (PCR) 180 7.2.1 The Principle of PCR 180 7.2.2 The Rate of Amplification During a PCR 183 7.2.3 Reagents for PCR 185 7.2.4 Real-Time PCR 187 7.2.5 Reverse Transcriptase — PCR (RT-PCR) 189 7.3 Nucleic Acid Sequencing 190 7.3.1 The use of Restriction Enzymes in Sequencing 190 7.3.2 The Chemical Cleavage Method (Maxam-Gilbert Method) 192 7.3.3 The Chain Terminator Method (Sanger or Dideoxy Method) 197 7.4 RNA-Sequencing 200 Summary 200 References 201 Chapter 8 Protein Analysis 203 8.1 Protein Sequencing Strategy 204 8.2 End-Group Analysis 204 8.2.1 N-terminal Analysis (Edman Degradation) 205 8.2.2 C-terminal Analysis 206 8.3 Disulfide-Bond Cleavage 210 8.4 Separation and Molecular Weight Determination of the Subunits 211 8.5 Amino Acid Composition 211 8.6 Cleavage of Specific Peptide Bond 214 8.6.1 Enzymatic Fragmentation 215 8.6.2 Chemical Fragmentation Methods 218 8.7 Sequence Determination 218 8.8 Ordering of Peptide-Fragments 220 8.9 Determination of Disulfide Bond Positions 220 8.10 Protein Sequencing by Mass Spectrometry 222 Summary 222 References 223 Index 225
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| discipline | Biologie Chemie |
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| illustrated | Illustrated |
| indexdate | 2024-07-10T07:09:24Z |
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| isbn | 9781783266715 9781783266722 |
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| physical | xx, 236 Seiten Illustrationen, Diagramme |
| publishDate | 2015 |
| publishDateSearch | 2015 |
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| publisher | Imperial College Press |
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| spelling | Manz, Andreas 1956- Verfasser (DE-588)121021661 aut Bioanalytical chemistry Andreas Manz, KIST Europe, Germany; Petra S Dittrich, ETH Zürich, Switzerland; Nicole Pamme, University of Hull, UK; Dimitri Iossifidis, Analytical Equipment Supplies & Support, Greece Second edition London Imperial College Press [2015] © 2015 xx, 236 Seiten Illustrationen, Diagramme txt rdacontent n rdamedia nc rdacarrier Biochemische Analyse (DE-588)4255721-5 gnd rswk-swf Biochemische Analyse (DE-588)4255721-5 s DE-604 Dittrich, Petra S. 1974- Verfasser (DE-588)128632682 aut Pamme, Nicole Verfasser (DE-588)1083014587 aut Iossifidis, Dimitri Verfasser (DE-588)1083014382 aut HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=028207666&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Manz, Andreas 1956- Dittrich, Petra S. 1974- Pamme, Nicole Iossifidis, Dimitri Bioanalytical chemistry Biochemische Analyse (DE-588)4255721-5 gnd |
| subject_GND | (DE-588)4255721-5 |
| title | Bioanalytical chemistry |
| title_auth | Bioanalytical chemistry |
| title_exact_search | Bioanalytical chemistry |
| title_full | Bioanalytical chemistry Andreas Manz, KIST Europe, Germany; Petra S Dittrich, ETH Zürich, Switzerland; Nicole Pamme, University of Hull, UK; Dimitri Iossifidis, Analytical Equipment Supplies & Support, Greece |
| title_fullStr | Bioanalytical chemistry Andreas Manz, KIST Europe, Germany; Petra S Dittrich, ETH Zürich, Switzerland; Nicole Pamme, University of Hull, UK; Dimitri Iossifidis, Analytical Equipment Supplies & Support, Greece |
| title_full_unstemmed | Bioanalytical chemistry Andreas Manz, KIST Europe, Germany; Petra S Dittrich, ETH Zürich, Switzerland; Nicole Pamme, University of Hull, UK; Dimitri Iossifidis, Analytical Equipment Supplies & Support, Greece |
| title_short | Bioanalytical chemistry |
| title_sort | bioanalytical chemistry |
| topic | Biochemische Analyse (DE-588)4255721-5 gnd |
| topic_facet | Biochemische Analyse |
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