Direct Microbial Conversion of Biomass to Advanced Biofuels:
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| Format: | Book |
| Language: | English |
| Published: |
Amsterdam [u.a.]
Elsevier
2015
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| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | XVIII, 403 Seiten Illustrationen, Diagramme |
| ISBN: | 9780444595928 |
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| 245 | 1 | 0 | |a Direct Microbial Conversion of Biomass to Advanced Biofuels |c edited by Michael E. Himmel |
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Record in the Search Index
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| adam_text | Titel: Direct microbial conversion of biomass to advanced biofuels
Autor: Himmel, Michael E
Jahr: 2015
Contents
Contributors....................................................................................................x/ii
Foreword.........................................................................................................xvii
Part 1: Direct Microbial Conversion of Biomass to Advanced Bio fuels.............1
Chapter 1: Feedstock Engineering and Biomass Pretreatments: New Views
for a Greener Biofuels Process..............................................................3
Feedstock Engineering Aiming to Provide More Pretreatable
and Digestable Biomass...............................................................................................3
In Planta Engineering for Reduced Recalcitrance Traits.............................................5
Mild and Green Pretreatments of Biomass for Lower Toxicity
in Lignocellulosic Hydrolysates and Solid Residues...................................................7
A New Concept of Tailored Chemoprocessing for Individual Microorganisms..........8
Building Unified Chemobiomass Databases and Libraries of Chemicals....................9
Conclusions................................................................................................................10
Acknowledgments......................................................................................................11
References..................................................................................................................11
Chapter 2: Hydrocarbon Biosynthesis in Microorganisms.......................................13
Introduction................................................................................................................13
Microbiology and Hydrocarbon Products..................................................................14
Enzymes and Mechanisms of Hydrocarbon Biosynthesis.........................................15
Ole-Catalyzed Synthesis of Long-Chain Olefins.....................................................15
The Chemistry of the Ole Gene Products................................................................18
OleA.........................................................................................................................19
OleD.........................................................................................................................24
OleC.........................................................................................................................25
OleB.........................................................................................................................25
Aldehyde Deformylating Oxygenase (Formerly Decarbonylase)..............................26
Alpha Olefins via Cytochrome P450.......................................................................... 27
Alpha Olefins via a Polyketide-Type Pathway...........................................................28
Conclusions................................................................................................................28
References..................................................................................................................29
v
vi Contents _________—————
Chapter 3: Perspectives on Process Analysis for Advanced Biofuel Production
Introduction....................................................................................................
Overview of Process Analysis.....................................................................
Aerobic Bioprocess........................................................................................
Process Design Details................................................................................
Aerobic Bioprocess Discussion......................................................................
Anaerobic Bioprocess....................................................................................
Process Design Details................................................................................
Anaerobic Bioprocess Discussion...............................................................
Consolidated Bioprocessing...........................................................................
Process Design Details................................................................................
CBP Discussion..........................................................................................
Data Gaps, Uncertainties, and Research Needs.............................................
Conclusion......................................................................................................
Acknowledgment............................................................................................
References......................................................................................................
Part 2: Biomass Structure and Recalcitrance..............................................61
Chapter 4: Tailoring Plant Cell Wall Composition and Architecture
for Conversion to Liquid Hydrocarbon Biofuels.....................................63
Biomass Feedstocks are Already an Abundant Resource...........................................64
Chemical Structure and Physical Properties of Lignocellulosic Biomass.................64
Biochemical, Chemical and Pyrolytic Conversion Pathways Provide
Alternative Routes to Fuels........................................................................................66
Tailoring Biomass for Downstream Conversion Processes........................................68
Adding Value to Plant Biomass Through Modification of Lignin..............................69
Redesigning Cellulose Microfibrils for Ease of Disassembly....................................72
Modification of Accessory Proteins for Altering Cellulose Microfibril Structure.....73
Modifying Xylan Composition and Architecture in the Interstitial Space.................73
Modulating Gene Expression Networks to Alter Lignin and Carbohydrate
Composition and Architecture....................................................................................75
Conclusions................................................................................................................76
References..................................................................................................................76
Chapter 5: Processive Celluloses.........................................................................S3
Acknowledgments......................................................................................................gg
References....................................................................................................................
Chapter 6: Bacterial AA10 Lytic Polysaccharide Monooxygenases Enhance
the Hydrolytic Degradation of Recalcitrant Substrates..........................97
Substrate Recalcitrance and Cellulase Mixtures........................................................91
Lytic Polysaccharide Monooxygenases.................................................................. 97
Conclusion..............................................................................................................................................................................jog
Acknowledgments............................................................................................................................................................jq7
References......................................
33
33
.34
39
.39
46
48
.48
.50
52
.52
.53
.55
56
.57
.57
Contents vii
Chapter 7: New Insights into Microbial Strategies for Biomass Conversion............111
Introduction..............................................................................................................Ill
Distinct Enzyme Synergy Paradigms in Ceilulolytic Microorganisms....................112
Free Enzyme Systems............................................................................................112
Self-Assembling, Highly Aggregated Enzyme Systems........................................116
Multifunctional Enzyme Systems..........................................................................117
New Cellulose Digestion Strategies Promoting Interspecies Synergism.................118
Cellulose Deconstruction by Cellulosomes: An Efficient and
Complementary Deconstruction Mechanism.........................................................119
The Hyperthermophilic Cellulase from Caldicellulosiruptor bescii CelA
Degrades Cellulose by Several Complementary Mechanisms...............................120
Future Perspective....................................................................................................121
Acknowledgments....................................................................................................125
References................................................................................................................125
Chapter 8: New Paradigms for Engineering Plant Cell Wall Degrading Enzymes.... 129
Introduction..............................................................................................................129
Engineering of Single Enzymes...............................................................................131
Cellulosome Engineering.........................................................................................132
Mini-Cellulosomes.................................................................................................132
Designer Cellulosomes..........................................................................................134
Cellulosome-Inspired Complexes..........................................................................136
Multifunctional Enzyme Design..............................................................................137
Cell Wall-Anchored Paradigms................................................................................139
Reflections and Perspectives....................................................................................141
Acknowledgments....................................................................................................144
References................................................................................................................144
Part 3: Fuels from Fungi and Yeast.........................................................151
Chapter 9: Expression of Fungal Hydrolases in Saccharomyces cerevisiae............153
Introduction..............................................................................................................153
Cellulose and Hemicellulose Structure and Hydrolysis...........................................154
Expression of Fungal Cellulases in Saccharomyces cerevisiae...............................157
Expression of Xylan Hydrolases in Saccharomyces cerevisiae...............................163
Expression of Mannan Hydrolases in Saccharomyces cerevisiae............................167
Discussion................................................................................................................168
References................................................................................................................169
Chapter 10: Identification of Genetic Targets to Improve Lignocellulosic
Hydrocarbon Production in Trichoderma reesei Using Public
Genomic and Transcriptomic Datasets.............................................177
Background..............................................................................................................177
Materials and Methods.............................................................................................179
Trichoderma reesei Protein Function Annotation and Pathway Reconstruction......179
Trichoderma reesei Microarray and RNA Sequencing Dataset Collection
and Transcriptomic Analyses.................................................................................179
viii Contents
101
Results and Discussions...........................................................................................xox
Identify Target Genes for Metabolic Engineering by Genomic Metabolic
Pathway Analysis....................................................................................•••••;.........J ^ 1
Identify Target Genes for Metabolic Engineering by Transcriptomic Analysis.......184
Investigate Transcriptional Regulators...................................................................188
Identify Promoters with Different Strength for Metabolic Engineering................189
Conclusions and Perspectives...................................................................................191
Acknowledgment......................................................................................................192
References................................................................................................................192
Chapter 11: Production ofEthanol from Engineered Trichoderma reesei.............197
Introduction..............................................................................................................197
Trichoderma reesei Produce Ethanol from Biomass Sugars....................................198
The pH during Fermentation Affects Ethanol Yield.................................................199
Sugar Used during Growth Phase Affects Xylose Fermentation.............................200
Direct Conversion of Cellulose to Ethanol...............................................................201
Enhancing Ethanol Synthesis by Metabolic Engineering........................................203
Discussion................................................................................................................204
Acknowledgment......................................................................................................206
References................................................................................................................206
Chapter 12: Remaining Challenges in the Metabolic Engineering of Yeasts
for Biofuels..................................................................................209
Introduction—Yeasts as the Catalyst for Biomass Consumption and Biofuel
Production................................................................................................................209
Metabolic Engineering—An Overview....................................................................211
Enzyme and Pathway Engineering...........................................................................212
Gene Expression Engineering..................................................................................214
Engineering the Metabolic Network—Classical Strain Engineering
and Systems Biology................................................................................................215
Computational Tools—Predictive Models for Metabolic Engineering....................216
Beyond Glucose.......................................................................................................216
Beyond Bioethanol...................................................................................................221
Beyond Current Capability.......................................................................................223
Beyond Saccharomyces cerevisiae...........................................................................225
Beyond Current Yield, Titers, and Production Rates................................................227
Conclusion................................................................................................................229
References................................................................................................................230
Part 4: Fuels from Bacteria....................................................................239
Chapter 13: New Tools for the Genetic Modification of Industrial Clostridia..........241
Introduction................................................................................................................................................................................................241
Transfer of Exogenous Genetic Material.........................................242
Electroporation................................................................................................................................................243
Conjugation....................................................................................................................................................243
Restriction-Modification Systems............................................................244
Contents ix
Clostridial Vector Systems.......................................................................................245
Forward Genetics by Random Mutagenesis.............................................................250
Random Mutagenesis by Chemical and Physical Mutagens.................................251
Random Mutagenesis by Biological Mutagens.....................................................252
Reverse Genetics......................................................................................................256
Recombination-Independent Methods (Group II Introns).....................................256
Recombination-Based Methods (Allelic Exchange)..............................................260
Other Advanced Genetic Tools.................................................................................269
Counter (Negative) Selection Markers...................................................................269
Inducible Gene Expression....................................................................................272
Conclusion................................................................................................................275
Acknowledgments....................................................................................................276
References................................................................................................................276
Chapter 14: Outlook for the Production of Butanol from Cellulolytic Strains
of Clostridia................................................................................291
Introduction..............................................................................................................291
Cellulolytic Clostridia and the Cellulosome............................................................291
Clostridium thermocellum.....................................................................................292
Clostridium cellulolyticum.....................................................................................293
Other Cellulolytic Hosts........................................................................................293
Microbial n-Butanol- and Isobutanol-Producing Pathways.....................................294
Microbial n-Butanol Pathways...............................................................................294
Microbial Isobutanol Pathways..............................................................................298
Progress toward Butanol CBP in Cellulolytic Clostridia.........................................301
Isobutanol CBP in Clostridium cellulolyticum......................................................301
Toward Isobutanol CBP in Clostridium thermocellum..........................................302
Clostridium acetobutylicum Cellulosome Development.......................................302
Conclusions..............................................................................................................302
Acknowledgment......................................................................................................303
References................................................................................................................303
Chapter IS: Influence of Particle Size on Direct Microbial Conversion of
Hot Water-Pretreated Poplar by Clostridium thermocellum............307
Introduction..............................................................................................................307
Materials and Methods.............................................................................................308
Microorganism and Fermentations........................................................................308
Substrate.................................................................................................................308
Compositional Analysis.........................................................................................309
Digestion Assay and Analysis................................................................................309
X-Ray Diffraction Measurements..........................................................................310
Growth Studies.......................................................................................................310
Nitrogen Analysis for Carbon:Nitrogen Ratio.......................................................311
Results......................................................................................................................311
Optimizing Growth Media for C. thermocellum Growth on Cellobiose,
Avicel, and Poplar..................................................................................................311
Particle Size Comparison (Poplar vs Avicel).........................................................313
x Contents
317
Conclusion................................................................................................................„
Acknowledgments....................................................................................................
References................................................................................................................
Chapter 16: Clostridium thermocellum: Engineered for the Production
of Bioethanol................................................................................^21
Biotechnological Interest in Clostridium thermocellum..........................................321
C. thermocellum Characteristics..............................................................................321
Ecology and Isolates.................................................................................................322
Physiology, Metabolism, and Ethanol Tolerance.....................................................323
Genome Sequences..................................................................................................325
Transcriptomics and Proteomics..............................................................................327
C. thermocellum Genetic Tools and Metabolic Engineering....................................328
Outlook.....................................................................................................................330
Acknowledgment......................................................................................................330
References................................................................................................................330
Chapter 17: Omics Approaches for Designing Biofuel Producing Cocultures
for Enhanced Microbial Conversion of Lignocellulosic Substrates........335
Introduction..............................................................................................................335
Synergistic Cocultures for Fermentation of Lignocellulosic Substrates..................336
Predicting Synergistic Cocultures............................................................................337
Taking Advantage of Omics to Understand Microbial Complementarity..........337
Complementarity in Glycoside Hydrolases and Hydrolysis of Complex
Substrates...............................................................................................................338
Carbohydrate Utilization in Firmicutes.................................................................344
Nutrient Complementation in Cellulolytic Cocultures..........................................347
Regulation of Microbial Interactions: Quorum Sensing........................................355
Conclusions..............................................................................................................358
References................................................................................................................359
Chapter 18: Engineering Synthetic Microbial Consortia for Consolidated
Bioprocessing of Lignocellulosic Biomass into Valuable Fuels
and Chemicals..............................................................................365
Introduction..............................................................................................................365
Engineering Single Microorganisms to Enable CBP...............................................367
Engineered Synthetic Microbial Consortia for CBP................................................369
Synthetic Consortia of Saccharification and Fermentation Specialists..................369
Other Synthetic Microbial Consortia for CBP.......................................................372
Emerging Methods for Designing and Regulating Synthetic Microbial
Consortia.....................................................................................................................
Synthetic Cell-Cell Signaling................................................................................374
Synthetic Ecologies..........................................................................................................................................................375
Elucidation for Engineering........................................................................................................................373
Concluding Remarks......................................................................................................................................................379
References..................................................................................................................................................................................379
Contents xi
Chapter 19: A Route from Biomass to Hydrocarbons via Depolymerization
and Decarboxylation of Microbially Produced Polyhydroxybutyrate.....383
Introduction..............................................................................................................383
Experimental Section...............................................................................................386
Chemicals and Catalysts........................................................................................386
Stainless Steel Tube Reactor..................................................................................386
Gas Analysis...........................................................................................................387
PHB Analysis.........................................................................................................387
Microbial Production of PHB................................................................................388
Results and Discussion.............................................................................................388
Thermal Decarboxylation of Crotonic Acid..........................................................388
Thermal Depolymerization and Decarboxylation of Commercial PHB................389
Microbial Production of PHB................................................................................389
Thermal Depolymerization and Decarboxylation of PHB Containing
Bacterial Cells........................................................................................................392
Conclusions..............................................................................................................392
Acknowledgment......................................................................................................393
References................................................................................................................393
Index..............................................................................................................395
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| spelling | Direct Microbial Conversion of Biomass to Advanced Biofuels edited by Michael E. Himmel Amsterdam [u.a.] Elsevier 2015 XVIII, 403 Seiten Illustrationen, Diagramme txt rdacontent n rdamedia nc rdacarrier Biomasse (DE-588)4006877-8 gnd rswk-swf Mikrobieller Abbau (DE-588)4039197-8 gnd rswk-swf Biokraftstoff (DE-588)4145658-0 gnd rswk-swf Biomasse (DE-588)4006877-8 s Mikrobieller Abbau (DE-588)4039197-8 s Biokraftstoff (DE-588)4145658-0 s DE-604 Himmel, Michael E. edt HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=028188171&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
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| title | Direct Microbial Conversion of Biomass to Advanced Biofuels |
| title_auth | Direct Microbial Conversion of Biomass to Advanced Biofuels |
| title_exact_search | Direct Microbial Conversion of Biomass to Advanced Biofuels |
| title_full | Direct Microbial Conversion of Biomass to Advanced Biofuels edited by Michael E. Himmel |
| title_fullStr | Direct Microbial Conversion of Biomass to Advanced Biofuels edited by Michael E. Himmel |
| title_full_unstemmed | Direct Microbial Conversion of Biomass to Advanced Biofuels edited by Michael E. Himmel |
| title_short | Direct Microbial Conversion of Biomass to Advanced Biofuels |
| title_sort | direct microbial conversion of biomass to advanced biofuels |
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