A transgenic probiotic bacterium as a carrier for a nematode immunomodulatory protein for the treatment of intestinal inflammation:
Saved in:
| Main Author: | |
|---|---|
| Format: | Thesis Book |
| Language: | English |
| Published: |
Berlin
Mensch und Buch Verl.
2014 [erschienen] 2015
|
| Subjects: | |
| Online Access: | Volltext Inhaltsverzeichnis |
| Item Description: | Nebentitel: Ein transgenes probiotisches Bakterium als ein Träger für ein immunmodulatorisches Nematoden-Protein für die Behandlung von intestinalen Entzündungen |
| Physical Description: | X, 116 S. Ill., graph. Darst. |
| ISBN: | 9783863875732 |
Staff View
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| 245 | 1 | 0 | |a A transgenic probiotic bacterium as a carrier for a nematode immunomodulatory protein for the treatment of intestinal inflammation |c vorgelegt von Rose Whelan |
| 246 | 1 | 3 | |a Ein transgenes probiotisches Bakterium als ein Träger für ein immunmodulatorisches Nematoden-Protein für die Behandlung von intestinalen Entzündungen |
| 264 | 1 | |a Berlin |b Mensch und Buch Verl. |c 2014 [erschienen] 2015 | |
| 300 | |a X, 116 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
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| 338 | |b nc |2 rdacarrier | ||
| 500 | |a Nebentitel: Ein transgenes probiotisches Bakterium als ein Träger für ein immunmodulatorisches Nematoden-Protein für die Behandlung von intestinalen Entzündungen | ||
| 502 | |a Berlin, Freie Univ., Diss., 2015 | ||
| 650 | 7 | |a inflammatory bowel diseases |2 cabt | |
| 650 | 7 | |a probiotics |2 cabt | |
| 650 | 7 | |a nematoda |2 cabt | |
| 650 | 7 | |a intestinal mucosa |2 cabt | |
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Record in the Search Index
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|---|---|
| adam_text | CONTENTS
CONTENTS
LIST OF TABLES VI
LIST OF FIGURES VII
LIST OF ABBREVIATIONS IX
1. INTRODUCTION 1
1.1. INFLAMMATORY BOWEL DISEASE 1
1.1.1. CAUSES AND DISEASE PATHOLOGY 1
1.1.2. MURINE MODELS OF IBD 2
1.1.3. CURRENT TREATMENT OPTIONS 3
1.2. HOST IMMUNE REGULATION BY PARASITIC NEMATODES 4
1.2.1. EVASION STRATEGIES BY NEMATODE PARASITES AND HOST IMMUNE RESPONSE
4
1.2.2. NEMATODE SUPPRESSION OF COLITIS 6
1.2.3. DEVELOPMENT OF NEMATODE CYSTATINS FOR IBD THERAPY 11
1.3. PROBIOTICS IN IBD TREATMENT 12
1.3.1. ESCHERICHIA COLI NISSLE 1917 13
1.3.2. TRANSGENIC PROBIOTICS 18
2. AIMS OF THE STUDY 20
3. RESULTS 22
3.1. TRANSGENIC E. COLI NISSLE EXPRESSES AND SECRETES ACANTHOCHEILONEMA
VITEAE
CYSTATIN 22
3.2. AVCYS SECRETION BY TRANSGENIC ECN-AVCYS INTO CELL CULTURE MEDIUM 24
3.3. TRANSGENIC ECN-AVCYS IS ABLE TO INHIBIT COLITIS IN MURINE MODELS OF
IBD 26
3.3.1. ECN-AVCYS IN THE DEXTRAN SODIUM SULFATE INDUCED CHRONIC COLITIS
MODEL 26
3.3.2. ECN-AVCYS INHIBITS THE DEXTRAN SODIUM SULFATE INDUCED ACUTE
COLITIS MODEL... 28
3.4. ECN-AVCYS IS WELL TOLERATED AND AMELIORATES POST-WEANING COLON
INFLAMMATION IN
PIGLETS 33
3.5. ECN-AVCYS SUPPORTS PORCINE GUT EPITHELIAL BARRIER FUNCTION 38
3.6. ECN-AVCYS SUPPORTS BARRIER FUNCTION OF HUMAN COLON EPITHELIAL CELLS
39
3.7. EXAMINATION OF CELLS AND MECHANISMS INVOLVED IN ECN-AVCYS IMMUNE
MODULATION 40
3.7.1. ECN-AVCYS MODULATION OF MONOCYTE AND MACROPHAGE PHENOTYPE 40
3.7.2. EFFECTS OF RAVCYS ON IL-1(3 SECRETION 45
4. DISCUSSION 47
HTTP://D-NB.INFO/107411390X
IV CONTENTS
4.1. AMELIORATION OF MURINE EXPERIMENTAL COLITIS BY ECN-AVCYS 47
4.2. ECN-AVCYS AMELIORATES PORCINE POST-WEANING GUT INFLAMMATION 53
4.3. ECN-AVCYS SUPPORTS THE INTESTINAL EPITHELIAL BARRIER 59
4.4. ECN AND ECN-AVCYS ALTER MACROPHAGE PHENOTYPE AND ACTIVATION 64
4.5. ECN-AVCYS DOES NOT INHIBIT MACROPHAGE OR MONOCYTE INFLAMMASOME
ACTIVATION... 70
5. CONCLUSIONS AND FUTURE PERSPECTIVES 75
6. SUMMARY 82
7. ZUSAMMENFASSUNG 84
8. METHODS 86
8.1. ETHICS STATEMENT 86
8.2. CONSTRUCTION OF THE TRANSGENIC PROBIOTIC 86
8.3. PRODUCTION OF ECN AND ECN-AVCYS SUPERNATANT 86
8.4. ANIMAL EXPERIMENTATION 87
8.4.1. MURINE DEXTRAN SODIUM SULFATE MODEL OF CHRONIC COLITIS 87
8.4.2. MURINE DEXTRAN SODIUM SULFATE MODEL OF ACUTE COLITIS 87
8.4.3. POST-WEANING PIGLET INTESTINAL INFLAMMATION MODEL 88
8.5. MOLECULAR DETECTION OF ECN-AVCYS IN MURINE FECES 88
8.6. CULTURE AND MOLECULAR DETECTION ATTEMPTS FOR ECN-AVCYS IN PORCINE
FECES 88
8.7. HISTOPATHOLOGICAL ANALYSIS AND IMMUNOHISTOCHEMISTRY 89
8.8. QUANTITATIVE REAL TIME PCR 90
8.9. QUANTIFICATION OF PIG BLOOD LEUKOCYTES 91
8.10. IMMUNE CELL ISOLATION AND ANALYSIS 91
8.10.1. CELL ISOLATION 91
8.10.2. CYTOKINE ANALYSIS 92
8.10.3. FLOW CYTOMETRY 93
8.10.4. THYMIDINE PROLIFERATION ASSAY 93
8.11. PRIMARY CELL CULTURE EXPERIMENTS 93
8.11.1. DIFFERENTIATION AND PHENOTYPE ANALYSIS OF PORCINE BLOOD
MONOCYTES 93
8.11.2. INFLAMMASOME INDUCTION IN HUMAN MONOCYTES AND MACROPHAGES 94
8.12. STATISTICS 94
9. REFERENCES 95
10. PUBLICATIONS, PATENTS AND CONFERENCE PROCEEDINGS 112
CONTENTS
V
11. ACKNOWLEDGEMENTS 113
12. AFFIDAVIT 116
|
| any_adam_object | 1 |
| author | Whelan, Rose |
| author_GND | (DE-588)1072114496 |
| author_facet | Whelan, Rose |
| author_role | aut |
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| dewey-full | 616.34406 |
| dewey-hundreds | 600 - Technology (Applied sciences) |
| dewey-ones | 616 - Diseases |
| dewey-raw | 616.34406 |
| dewey-search | 616.34406 |
| dewey-sort | 3616.34406 |
| dewey-tens | 610 - Medicine and health |
| discipline | Medizin |
| format | Thesis Book |
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| spelling | Whelan, Rose Verfasser (DE-588)1072114496 aut A transgenic probiotic bacterium as a carrier for a nematode immunomodulatory protein for the treatment of intestinal inflammation vorgelegt von Rose Whelan Ein transgenes probiotisches Bakterium als ein Träger für ein immunmodulatorisches Nematoden-Protein für die Behandlung von intestinalen Entzündungen Berlin Mensch und Buch Verl. 2014 [erschienen] 2015 X, 116 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Nebentitel: Ein transgenes probiotisches Bakterium als ein Träger für ein immunmodulatorisches Nematoden-Protein für die Behandlung von intestinalen Entzündungen Berlin, Freie Univ., Diss., 2015 inflammatory bowel diseases cabt probiotics cabt nematoda cabt intestinal mucosa cabt Antiphlogistikum (DE-588)4123144-2 gnd rswk-swf Chronische Darmentzündung (DE-588)4273697-3 gnd rswk-swf Probiotikum (DE-588)4338112-1 gnd rswk-swf Escherichia coli (DE-588)4070959-0 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Chronische Darmentzündung (DE-588)4273697-3 s Probiotikum (DE-588)4338112-1 s Escherichia coli (DE-588)4070959-0 s Antiphlogistikum (DE-588)4123144-2 s DE-604 Erscheint auch als Online-Ausgabe urn:nbn:de:kobv:188-fudissthesis000000099353-3 http://www.diss.fu-berlin.de/diss/receive/FUDISS_thesis_000000099353 Resolving-System kostenfrei Volltext DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=028038989&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Whelan, Rose A transgenic probiotic bacterium as a carrier for a nematode immunomodulatory protein for the treatment of intestinal inflammation inflammatory bowel diseases cabt probiotics cabt nematoda cabt intestinal mucosa cabt Antiphlogistikum (DE-588)4123144-2 gnd Chronische Darmentzündung (DE-588)4273697-3 gnd Probiotikum (DE-588)4338112-1 gnd Escherichia coli (DE-588)4070959-0 gnd |
| subject_GND | (DE-588)4123144-2 (DE-588)4273697-3 (DE-588)4338112-1 (DE-588)4070959-0 (DE-588)4113937-9 |
| title | A transgenic probiotic bacterium as a carrier for a nematode immunomodulatory protein for the treatment of intestinal inflammation |
| title_alt | Ein transgenes probiotisches Bakterium als ein Träger für ein immunmodulatorisches Nematoden-Protein für die Behandlung von intestinalen Entzündungen |
| title_auth | A transgenic probiotic bacterium as a carrier for a nematode immunomodulatory protein for the treatment of intestinal inflammation |
| title_exact_search | A transgenic probiotic bacterium as a carrier for a nematode immunomodulatory protein for the treatment of intestinal inflammation |
| title_full | A transgenic probiotic bacterium as a carrier for a nematode immunomodulatory protein for the treatment of intestinal inflammation vorgelegt von Rose Whelan |
| title_fullStr | A transgenic probiotic bacterium as a carrier for a nematode immunomodulatory protein for the treatment of intestinal inflammation vorgelegt von Rose Whelan |
| title_full_unstemmed | A transgenic probiotic bacterium as a carrier for a nematode immunomodulatory protein for the treatment of intestinal inflammation vorgelegt von Rose Whelan |
| title_short | A transgenic probiotic bacterium as a carrier for a nematode immunomodulatory protein for the treatment of intestinal inflammation |
| title_sort | a transgenic probiotic bacterium as a carrier for a nematode immunomodulatory protein for the treatment of intestinal inflammation |
| topic | inflammatory bowel diseases cabt probiotics cabt nematoda cabt intestinal mucosa cabt Antiphlogistikum (DE-588)4123144-2 gnd Chronische Darmentzündung (DE-588)4273697-3 gnd Probiotikum (DE-588)4338112-1 gnd Escherichia coli (DE-588)4070959-0 gnd |
| topic_facet | inflammatory bowel diseases probiotics nematoda intestinal mucosa Antiphlogistikum Chronische Darmentzündung Probiotikum Escherichia coli Hochschulschrift |
| url | http://www.diss.fu-berlin.de/diss/receive/FUDISS_thesis_000000099353 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=028038989&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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