Low-cost methods for molecular characterization of mutant plants: tissue desiccation, DNA extraction and mutation discovery - protocols
Saved in:
| Format: | Electronic eBook |
|---|---|
| Language: | English |
| Published: |
Cham [u.a.]
Springer Open
2015
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| Subjects: | |
| Online Access: | Volltext Inhaltsverzeichnis Abstract |
| Physical Description: | 1 Online-Ressource (X, 35 S.) Ill. |
| ISBN: | 9783319162591 |
| DOI: | 10.1007/978-3-319-16259-1 |
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| 650 | 4 | |a Biological Techniques | |
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Record in the Search Index
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| adam_text | LOW-COST METHODS FOR MOLECULAR CHARACTERIZATION OF MUTANT PLANTS
/ TILL, BRADLEY J.
: 2015
TABLE OF CONTENTS / INHALTSVERZEICHNIS
INTRODUCTION
HEALTH AND SAFETY CONSIDERATIONS
SAMPLE COLLECTION AND STORAGE
LOW-COST DNA EXTRACTION
PCR AMPLIFICATION FOR LOW-COST MUTATION DISCOVERY
ENZYMATIC MISMATCH CLEAVAGE AND AGAROSE GEL EVALUATION OF SAMPLES
ALTERNATIVE ENZYMOLOGY FOR MISMATCH CLEAVAGE FOR TILLING AND ECOTILLING:
EXTRACTION OF ENZYMES FORM COMMON WEEDY PLANTS
EXAMPLE DATA
CONCLUSIONS.
DIESES SCHRIFTSTUECK WURDE MASCHINELL ERZEUGT.
LOW-COST METHODS FOR MOLECULAR CHARACTERIZATION OF MUTANT PLANTS
/ TILL, BRADLEY J.
: 2015
ABSTRACT / INHALTSTEXT
THIS BOOK OFFERS LOW-COST AND RAPID MOLECULAR ASSAYS FOR THE
CHARACTERIZATION OF MUTANT PLANT GERMPLASM. DETAILED PROTOCOLS ARE
PROVIDED FOR THE DESICCATION OF PLANT TISSUES; THE EXTRACTION OF
HIGH-QUALITY DNA FOR DOWNSTREAM APPLICATIONS; THE EXTRACTION OF
SINGLE-STRAND-SPECIFIC NUCLEASES FOR SINGLE NUCLEOTIDE POLYMORPHISM; AND
SMALL INSERTION/DELETION DISCOVERY USING STANDARD AGAROSE GEL
ELECTROPHORESIS. THE METHODS DESCRIBED CAN BE APPLIED IN ANY LABORATORY
EQUIPPED FOR BASIC MOLECULAR BIOLOGY AND DO AWAY WITH THE NEED FOR
EXPENSIVE FREEZERS AND TOXIC ORGANIC COMPOUNDS. WITH THE APPROPRIATE
VALIDATION OF SAMPLE QUALITY AND LONGEVITY, THEY CAN PROVIDE SUFFICIENT
DNA FOR A VARIETY OF MOLECULAR APPLICATIONS, SUCH AS MARKER STUDIES AND
TILLING, AT APPROXIMATELY ONE TENTH OF THE COST PER SAMPLE WHEN COMPARED
TO COMMERCIAL KITS
DIESES SCHRIFTSTUECK WURDE MASCHINELL ERZEUGT.
|
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| bvnumber | BV042592154 |
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| ctrlnum | (OCoLC)1073737259 (DE-599)BVBBV042592154 |
| dewey-full | 631.52 660.6 |
| dewey-hundreds | 600 - Technology (Applied sciences) |
| dewey-ones | 631 - Techniques, equipment & materials 660 - Chemical engineering |
| dewey-raw | 631.52 660.6 |
| dewey-search | 631.52 660.6 |
| dewey-sort | 3631.52 |
| dewey-tens | 630 - Agriculture and related technologies 660 - Chemical engineering |
| discipline | Chemie / Pharmazie Biologie Agrar-/Forst-/Ernährungs-/Haushaltswissenschaft / Gartenbau |
| doi_str_mv | 10.1007/978-3-319-16259-1 |
| format | Electronic eBook |
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| id | DE-604.BV042592154 |
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| indexdate | 2024-07-10T07:05:25Z |
| institution | BVB |
| isbn | 9783319162591 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-028025366 |
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| physical | 1 Online-Ressource (X, 35 S.) Ill. |
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| spelling | Low-cost methods for molecular characterization of mutant plants tissue desiccation, DNA extraction and mutation discovery - protocols Bradley J. Till ... Cham [u.a.] Springer Open 2015 1 Online-Ressource (X, 35 S.) Ill. txt rdacontent c rdamedia cr rdacarrier Life sciences Nucleic acids Plant breeding Cytology / Research_xMethodology Life Sciences Plant Breeding/Biotechnology Biological Techniques Nucleic Acid Chemistry Biowissenschaften Till, Bradley J. Sonstige oth Erscheint auch als Druckausgabe 978-3-319-16258-4 https://doi.org/10.1007/978-3-319-16259-1 Verlag kostenfrei Volltext Springer Fremddatenuebernahme application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=028025366&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis Springer Fremddatenuebernahme application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=028025366&sequence=000003&line_number=0002&func_code=DB_RECORDS&service_type=MEDIA Abstract |
| spellingShingle | Low-cost methods for molecular characterization of mutant plants tissue desiccation, DNA extraction and mutation discovery - protocols Life sciences Nucleic acids Plant breeding Cytology / Research_xMethodology Life Sciences Plant Breeding/Biotechnology Biological Techniques Nucleic Acid Chemistry Biowissenschaften |
| title | Low-cost methods for molecular characterization of mutant plants tissue desiccation, DNA extraction and mutation discovery - protocols |
| title_auth | Low-cost methods for molecular characterization of mutant plants tissue desiccation, DNA extraction and mutation discovery - protocols |
| title_exact_search | Low-cost methods for molecular characterization of mutant plants tissue desiccation, DNA extraction and mutation discovery - protocols |
| title_full | Low-cost methods for molecular characterization of mutant plants tissue desiccation, DNA extraction and mutation discovery - protocols Bradley J. Till ... |
| title_fullStr | Low-cost methods for molecular characterization of mutant plants tissue desiccation, DNA extraction and mutation discovery - protocols Bradley J. Till ... |
| title_full_unstemmed | Low-cost methods for molecular characterization of mutant plants tissue desiccation, DNA extraction and mutation discovery - protocols Bradley J. Till ... |
| title_short | Low-cost methods for molecular characterization of mutant plants |
| title_sort | low cost methods for molecular characterization of mutant plants tissue desiccation dna extraction and mutation discovery protocols |
| title_sub | tissue desiccation, DNA extraction and mutation discovery - protocols |
| topic | Life sciences Nucleic acids Plant breeding Cytology / Research_xMethodology Life Sciences Plant Breeding/Biotechnology Biological Techniques Nucleic Acid Chemistry Biowissenschaften |
| topic_facet | Life sciences Nucleic acids Plant breeding Cytology / Research_xMethodology Life Sciences Plant Breeding/Biotechnology Biological Techniques Nucleic Acid Chemistry Biowissenschaften |
| url | https://doi.org/10.1007/978-3-319-16259-1 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=028025366&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=028025366&sequence=000003&line_number=0002&func_code=DB_RECORDS&service_type=MEDIA |
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