Small non-coding RNAs: methods and protocols
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| Format: | Book |
|---|---|
| Language: | English |
| Published: |
Totowa, N.J. [u.a.]
Humana Press
2015
|
| Series: | Methods in molecular biology
1296 |
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | XI, 238 S. Ill., graph. Darst. |
| ISBN: | 9781493925469 |
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| 245 | 1 | 0 | |a Small non-coding RNAs |b methods and protocols |c ed. by Mathieu Rederstorff |
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Record in the Search Index
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| adam_text | Titel: Small non-coding RNAs
Autor: Rederstorff, Mathieu
Jahr: 2015
Contents
Preface.......................................................... v
Contributors.......................................................... ix
Part I Introduction to Small Non-Coding RNAs
1 Small Non-Coding RNAs: A Quick Look in the Rear view Mirror.......... 3
Guillaume Clerget, Toann Abel, and Mathieu Rederstorjf
2 Alcoholic Precipitation of Small Non-Coding RNAs.................... 11
Guillaume Clerget, VaUrie Bourguignon-Igel, and Mathieu Rederstorjf
3 Quantification and Quality Control of a Small Non-Coding
RNA Preparation.............................................. 17
Virginie Marchand and Christiane Branlant
4 Impact of RNA Isolation Protocols on RNA Detection
by Northern Blotting........................................... 29
Katrin Damm, Simone Bach, Katrin M.H. Milller, Gabriele Klug,
Olga T. Burenina, Elena A. Kubareva, Arnold Grunweller,
and Roland K. Hartmann
Part II Visualization and Analysis of Small Non-Coding RNAs
5 Improved Northern Blot Detection of Small RNAs Using EDC
Crosslinking and DNA/LNA Probes................................ 41
Katrin Damm, Simone Bach, Katrin M.H. Milller, Gabriele Klug,
Olga T. Burenina, Elena A. Kubareva, Arnold Grilnweller,
and Roland K. Hartmann
6 Direct Cloning of Double-Stranded RNAs........................... 53
Manli Shen, Marina Ealaleeva, Natalia Korotkova, and Stefan Stamm
7 Detection and Labeling of Small Non-Coding RNAs by Splinted Ligation. ... 65
Gabrielle Bourgeois, Florian Chardon, Anne-Sophie Tillault,
and Magali Blaud
8 Fluorescence In Situ Hybridization of Small Non-Coding RNAs........... 73
Valentin Vautrot, Christelle Aigueperse, Christiane Branlant,
and Isabelle Behm-Ansmant
9 RT-qPCR-Based Quantification of Small Non-Coding RNAs............. 85
Fjoralba Zeka, Pieter Mestdagh, and Jo Vandesompele
10 Stem-Loop RT-PCR Based Quantification of Small Non-Coding RNAs..... 103
Veronique Salone and Mathieu Rederstorjf
11 miR-RACE: An Effective Approach to Accurately Determine
the Sequence of Computationally Identified miRNAs................... 109
Chen Wang and Jinggui Fang
vii
viii Contents
12 Probing Small Non-Coding RNAs Structures......................... 119
Jean-Vincent Philippe, Lilia Ayadi, Christiane Branlant,
and Isabelle Behm-Ansmant
Part III High-Throughput Approaches to Study Non-Coding RNAs
13 cDNA Library Generation for the Analysis of Small RNAs
by High-Throughput Sequencing.................................. 139
Jennifer Gebetsberger, Roger Pricker, and Norbert Polacek
14 CLIP-Seq to Discover Transcriptome-Wide Imprinting
of RNA Binding Proteins in Living Cells............................. 151
Jerome Sauliere and Herve Le Hir
15 Microarray Analysis of Small Non-Coding RNAs....................... 161
Michael Karbiener and Marcel Scheideler
Part IV Small Non-Coding RNAs Applications
16 RLM-RACE, PPM-RACE, and qRT-PCRj An Integrated Strategy
to Accurately Validate miRNA Target Genes.......................... 175
Chen Wang and Jinggui Fang
17 Dual Luciferase Gene Reporter Assays to Study miRNA Function.......... 187
Thomas CUment, Veronique Salone, and Mathieu Rjederstorjf
18 Gene Expression Knockdown by Transfection of siRNAs
into Mammalian Cells........................................... 199
Toann Abel and Mathieu Rederstorff
19 Efficient and Selective Knockdown of Small Non-Coding RNAs........... 203
Xue-Hai Liang, Wen Shen, and Stanley T Crooke
20 Cell-SELEX: In Vitro Selection of Synthetic Small Specific Ligands......... 213
Helena Dickinson, Melanie Lukasser, Giinter Mayer,
and Alexander Hllttenhofer
21 Small Non-Coding RNAs and Aptamers in Diagnostics and Therapeutics .... 225
Marissa Leonard, Tijuan Zhang, and Xiaoting Zhang
Index............................................................... 235
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| isbn | 9781493925469 |
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| physical | XI, 238 S. Ill., graph. Darst. |
| publishDate | 2015 |
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| series | Methods in molecular biology |
| series2 | Methods in molecular biology Springer protocols |
| spelling | Small non-coding RNAs methods and protocols ed. by Mathieu Rederstorff Totowa, N.J. [u.a.] Humana Press 2015 XI, 238 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 1296 Springer protocols Methode (DE-588)4038971-6 gnd rswk-swf Small RNA (DE-588)7501213-3 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Small RNA (DE-588)7501213-3 s Methode (DE-588)4038971-6 s DE-604 Rederstorff, Mathieu 1979- Sonstige (DE-588)1069793280 oth Methods in molecular biology 1296 (DE-604)BV035362695 1296 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027977807&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Small non-coding RNAs methods and protocols Methods in molecular biology Methode (DE-588)4038971-6 gnd Small RNA (DE-588)7501213-3 gnd |
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| title | Small non-coding RNAs methods and protocols |
| title_auth | Small non-coding RNAs methods and protocols |
| title_exact_search | Small non-coding RNAs methods and protocols |
| title_full | Small non-coding RNAs methods and protocols ed. by Mathieu Rederstorff |
| title_fullStr | Small non-coding RNAs methods and protocols ed. by Mathieu Rederstorff |
| title_full_unstemmed | Small non-coding RNAs methods and protocols ed. by Mathieu Rederstorff |
| title_short | Small non-coding RNAs |
| title_sort | small non coding rnas methods and protocols |
| title_sub | methods and protocols |
| topic | Methode (DE-588)4038971-6 gnd Small RNA (DE-588)7501213-3 gnd |
| topic_facet | Methode Small RNA Aufsatzsammlung |
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