Effect of autoantibodies targeting amphiphysin or glutamate decarboxylase 65 on synaptic transmission of GABAergic neurons:
Saved in:
| Main Author: | |
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| Format: | Thesis Book |
| Language: | English |
| Published: |
2014
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| Subjects: | |
| Online Access: | Volltext Inhaltsverzeichnis |
| Item Description: | Zsfassung in dt. und engl. Sprache |
| Physical Description: | IV, 65 Bl. Ill., graph. Darst. |
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| 245 | 1 | 0 | |a Effect of autoantibodies targeting amphiphysin or glutamate decarboxylase 65 on synaptic transmission of GABAergic neurons |c von Christian Werner |
| 246 | 1 | 3 | |a Einfluss von Autoantikörpern gegen Amphiphysin oder Glutamatdecarboxylase 65 auf synaptische Transmission GABAerger Neurone |
| 264 | 1 | |c 2014 | |
| 300 | |a IV, 65 Bl. |b Ill., graph. Darst. | ||
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Record in the Search Index
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| adam_text | EFFECT OF AUTOANTIBODIES TARGETING AMPHIPHYSIN OR GLUTAMATE
DECARBOXYLASE
65 ON SYNAPTIC TRANSMISSION OF GABAERGIC NEURONS
EINFLUSS VON AUTOANTIKORPERN GEGEN AMPHIPHYSIN ODER
GLUTAMATDECARBOXYLASE 65 AUF SYNAPTISCHE TRANSMISSION
GABAERGER NEURONE
DOCTORAL THESIS FOR A DOCTORAL DEGREE
AT THE GRADUATE SCHOOL OF LIFE SCIENCES,
JULIUS-MAXIMILIANS-UNIVERSITAT WURZBURG,
SECTION NEUROSCIENCE
SUBMITTED BY
CHRISTIAN WERNER
FROM
SCHWEINFURT
WURZBURG, 2014
HTTP://D-NB.INFO/1066683379
INDEX
SUMMARY I
ZUSAMMENFASSUNG III
INDEX V
1. INTRODUCTION 1
1.1. HUMORAL AUTOIMMUNITY IN THE CNS 1
1.2. STIFF PERSON SYNDROME 2
1.3. CHEMICAL SYNAPSES AND THE VESICLE CYCLE 4
1.4. AMPHIPHYSIN 5
1.5. SCIENTIFIC RESEARCH QUESTION: ANTI-AMPHIPHYSIN AB 8
1.6. GLUTAMATE DECARBOXYLASE 65 (GAD65) 8
1.7. SCIENTIFIC RESEARCH QUESTION: ANTI-GAD65 AB 9
2. MATERIAL AND METHODS 10
2.1. ANIMAL EXPERIMENTS 10
2.2. PATIENT IGG AND AB PURIFICATION 10
2.3. PRIMARY HIPPOCAMPAL CELL CULTURE 11
2.4. IN-VIVO RAT MODEL OF SPS 12
2.5. ELECTRON MICROSCOPY OF RAT SPINAL TERMINALS 13
2.6. T/STORM MICROSCOPY EXPERIMENTS 14
2.7. CONFOCAL MICROSCOPY FOR ANALYSIS OF GABAERGIC BOUTON SIZE AND IGG
BINDING
EXPERIMENTS 16
2.8. WHOLE CELL PATCH-CLAMP RECORDINGS 17
2.9. QUANTITATIVE REAL TIME POLYMERASE CHAIN REACTION (QRT-PCR) 18
2.10. STATISTICS 19
3. RESULTS A: IMPACT OF SPS ANTI-AMPHIPHYSIN AB ON SYNAPTIC STRUCTURE 20
3.1. SUSTAINED STIMULATION INCREASES SYNAPTIC VESICLE POOL SIZE AND THE
AMOUNT OF CLATHRIN-
COATED VESICLES IN NAIVE GABAERGIC SPINAL CORD INTERNEURONS 20
3.2. PATHOGENIC HUMAN ANTI-AMPHIPHYSIN AB INTERFERE WITH CME LEADING TO
STIMULUS-
DEPENDENT PRESYNAPTIC VESICLE DEPLETION 23
3.3. DIFFERENT V-SNARE COMPOSITION OF GABAERGIC VESICLE POOLS INDUCED BY
ANTI-
AMPHIPHYSIN AB 28
3.4. HUMAN ABTO AMPHIPHYSIN ALTER ENDOPHILIN DENSITY AND CLUSTERING IN
GABAERGIC
PRESYNAPTIC VESICLE POOLS 31
4. RESULTS B: FUNCTIONAL CONSEQUENCES INDUCED BY ANTI-GAD65 CONTAINING
SPS-IGG 36
4.1. NORMAL BASAL GABAERGIC NEUROTRANSMISSION IN PRESENCE OF ANTI-GAD65
AB CONTAINING
SPS-IGG 36
4.2. NORMAL SHORT TERM PLASTICITY AND HIGH FREQUENCY TRANSMISSION AFTER
ANTI-GAD65 IGG
INCUBATION 37
4.3. ANTI-GAD65 AB CONTAINING IGG INCREASES FREQUENCY OF MLPSCS 38
4.4. MICROISLAND CULTURES AS MODEL SYSTEM TO INVESTIGATE FUNCTIONAL
EFFECTS OF PATHOGENIC
ANTINEURONAL AB 42
5. DISCUSSION 44
5.1. PATHOPHYSIOLOGICAL MECHANISMS OF ANTI-AMPHIPHYSIN AB 44
5.2. THE ROLE OF ANTI-GAD65 AB IN SPS 46
REFERENCE LIST 49
APPENDIX A: INDEX OF FIGURES 56
APPENDIX B: ABBREVIATIONS 58
AFFIDAVIT & EIDESSTATTLICHE ERKLARUNG 61
DANKSAGUNG/ACKNOWLEDGEMENTS (IN GERMAN) 62
PUBLICATIONS 64
CURRICULUM VITAE 65
|
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| indexdate | 2024-07-10T01:20:37Z |
| institution | BVB |
| language | English |
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| spelling | Werner, Christian Verfasser (DE-588)1068340886 aut Effect of autoantibodies targeting amphiphysin or glutamate decarboxylase 65 on synaptic transmission of GABAergic neurons von Christian Werner Einfluss von Autoantikörpern gegen Amphiphysin oder Glutamatdecarboxylase 65 auf synaptische Transmission GABAerger Neurone 2014 IV, 65 Bl. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Zsfassung in dt. und engl. Sprache Würzburg, Univ., Diss., 2014 Autoaggressionskrankheit (DE-588)4003935-3 gnd rswk-swf Glutamat-Decarboxylase (DE-588)4157706-1 gnd rswk-swf Synapse (DE-588)4184224-8 gnd rswk-swf Zentralnervensystem (DE-588)4067637-7 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Autoaggressionskrankheit (DE-588)4003935-3 s Zentralnervensystem (DE-588)4067637-7 s Synapse (DE-588)4184224-8 s Glutamat-Decarboxylase (DE-588)4157706-1 s DE-604 Erscheint auch als Online-Ausgabe urn:nbn:de:bvb:20-opus-105648 https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-105648 Resolving-System kostenfrei Volltext DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027840284&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Werner, Christian Effect of autoantibodies targeting amphiphysin or glutamate decarboxylase 65 on synaptic transmission of GABAergic neurons Autoaggressionskrankheit (DE-588)4003935-3 gnd Glutamat-Decarboxylase (DE-588)4157706-1 gnd Synapse (DE-588)4184224-8 gnd Zentralnervensystem (DE-588)4067637-7 gnd |
| subject_GND | (DE-588)4003935-3 (DE-588)4157706-1 (DE-588)4184224-8 (DE-588)4067637-7 (DE-588)4113937-9 |
| title | Effect of autoantibodies targeting amphiphysin or glutamate decarboxylase 65 on synaptic transmission of GABAergic neurons |
| title_alt | Einfluss von Autoantikörpern gegen Amphiphysin oder Glutamatdecarboxylase 65 auf synaptische Transmission GABAerger Neurone |
| title_auth | Effect of autoantibodies targeting amphiphysin or glutamate decarboxylase 65 on synaptic transmission of GABAergic neurons |
| title_exact_search | Effect of autoantibodies targeting amphiphysin or glutamate decarboxylase 65 on synaptic transmission of GABAergic neurons |
| title_full | Effect of autoantibodies targeting amphiphysin or glutamate decarboxylase 65 on synaptic transmission of GABAergic neurons von Christian Werner |
| title_fullStr | Effect of autoantibodies targeting amphiphysin or glutamate decarboxylase 65 on synaptic transmission of GABAergic neurons von Christian Werner |
| title_full_unstemmed | Effect of autoantibodies targeting amphiphysin or glutamate decarboxylase 65 on synaptic transmission of GABAergic neurons von Christian Werner |
| title_short | Effect of autoantibodies targeting amphiphysin or glutamate decarboxylase 65 on synaptic transmission of GABAergic neurons |
| title_sort | effect of autoantibodies targeting amphiphysin or glutamate decarboxylase 65 on synaptic transmission of gabaergic neurons |
| topic | Autoaggressionskrankheit (DE-588)4003935-3 gnd Glutamat-Decarboxylase (DE-588)4157706-1 gnd Synapse (DE-588)4184224-8 gnd Zentralnervensystem (DE-588)4067637-7 gnd |
| topic_facet | Autoaggressionskrankheit Glutamat-Decarboxylase Synapse Zentralnervensystem Hochschulschrift |
| url | https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-105648 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027840284&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT wernerchristian effectofautoantibodiestargetingamphiphysinorglutamatedecarboxylase65onsynaptictransmissionofgabaergicneurons AT wernerchristian einflussvonautoantikorperngegenamphiphysinoderglutamatdecarboxylase65aufsynaptischetransmissiongabaergerneurone |