Regulatory Non-Coding RNAs: methods and protocols
Saved in:
| Format: | Book |
|---|---|
| Language: | English |
| Published: |
Totowa, NJ
Humana Press
2015
|
| Series: | Methods in molecular biology
1206 |
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | X, 181 S. Ill. |
| ISBN: | 9781493913688 |
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Record in the Search Index
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| adam_text | Titel: Regulatory non-coding RNAS
Autor: Carmichael, Gordon G
Jahr: 2015
Contents
Preface.............................................................. v
Contributors.......................................................... ix
1 Diverse Functions and Mechanisms of Mammalian
Long Noncoding RNAs......................................... 1
Callie R. Merry, Courtney Niland, and Ahmad M. Khalil
2 Sequence-Defined Oligoaminoamides for the Delivery of siRNAs.......... 15
Dian-Jang Lee, Ernst Wagner, and Taavi Lehto
3 A Biochemical Approach to Identify Direct MicroRNA Targets............ 29
Murugan Subramanian, Xiao Ling Li, Toshifumi Hara, and Ashish Lai
4 Native Gel Analysis for Mammalian MicroRNPs Assembled from
Pre-MicroRNAs............................................... 39
Xuhang Liu and Zissimos Mourelatos
5 Identification and Validation of miRNA Target Sites Within Nontraditional
miRNA Targets................................................ 53
Scott T. Younger and David R. Corey
6 Fractionation of Non-polyadenylated and Ribosomal-Free RNAs
from Mammalian Cells.......................................... 69
Qing-Fei Yin, Ling-Ling Chen, and Li Yang
7 Affinity Purification of Long Noncoding RNA-Protein Complexes
from Formaldehyde Cross-Linked Mammalian Cells.................... 81
Chenguang Gong and Lynne E. Maquat
8 RNA Pulldown Protocol for In Vitro Detection and Identification
of RNA-Associated Proteins...................................... 87
Oskar Marin-Béjar and Maite Huarte
9 nRIP-seq: A Technique to Identify RNA Targets of an RNA Binding
Protein on a Genome-Wide Scale.................................. 97
Jing Crystal Zhao
10 Analysis of the Subcellular Distribution of RNA by Fluorescence
In Situ Hybridization........................................... 107
Shinichi Nakagawa
11 RNA Fluorescence In Situ Hybridization in Cultured Mammalian Cells..... 123
Vidisha Tripathi, Jingyi Fei, Taekjip Ha, and Kannanganattu V. Prasanth
12 Locating RNAs In Situ with FISH-STIC Probes....................... 137
John R. Sinnamon and Kevin Czaplinski
13 Rapid and Efficient Elimination of Specific Nuclear Noncoding RNAs
in Mammalian Cells with Antisense Oligonucleotides................... 149
Tetsuro Hirose and Taro Mannen
vii
viii Contents
14 On Using Magnesium and Potassium Ions in RNA Experiments........... 157
Neena Grover
15 Studying the Affinity, Kinetic Stability, and Specificity of RNA/Protein
Interactions: SINE ncRNA/Pol II Complexes as a Model System.......... 165
James A. Goodrich and Jennifer F. Kugel
Index............................................................... 179
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| physical | X, 181 S. Ill. |
| publishDate | 2015 |
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| series | Methods in molecular biology |
| series2 | Methods in molecular biology |
| spelling | Regulatory Non-Coding RNAs methods and protocols ed. by Gordon G. Carmichael Totowa, NJ Humana Press 2015 X, 181 S. Ill. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 1206 Non-coding RNA (DE-588)7650051-2 gnd rswk-swf Regulation (DE-588)4049075-0 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Non-coding RNA (DE-588)7650051-2 s Regulation (DE-588)4049075-0 s DE-604 Carmichael, Gordon G. Sonstige oth Methods in molecular biology 1206 (DE-604)BV035362695 1206 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027799112&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Regulatory Non-Coding RNAs methods and protocols Methods in molecular biology Non-coding RNA (DE-588)7650051-2 gnd Regulation (DE-588)4049075-0 gnd |
| subject_GND | (DE-588)7650051-2 (DE-588)4049075-0 (DE-588)4143413-4 |
| title | Regulatory Non-Coding RNAs methods and protocols |
| title_auth | Regulatory Non-Coding RNAs methods and protocols |
| title_exact_search | Regulatory Non-Coding RNAs methods and protocols |
| title_full | Regulatory Non-Coding RNAs methods and protocols ed. by Gordon G. Carmichael |
| title_fullStr | Regulatory Non-Coding RNAs methods and protocols ed. by Gordon G. Carmichael |
| title_full_unstemmed | Regulatory Non-Coding RNAs methods and protocols ed. by Gordon G. Carmichael |
| title_short | Regulatory Non-Coding RNAs |
| title_sort | regulatory non coding rnas methods and protocols |
| title_sub | methods and protocols |
| topic | Non-coding RNA (DE-588)7650051-2 gnd Regulation (DE-588)4049075-0 gnd |
| topic_facet | Non-coding RNA Regulation Aufsatzsammlung |
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