The proteome revisited: theory and practice of all relevant electrophoretic steps
Saved in:
| Main Author: | |
|---|---|
| Format: | Electronic eBook |
| Language: | English |
| Published: |
Amsterdam
Elsevier Science
2001
|
| Edition: | 1st ed |
| Series: | Journal of chromatography library
v. 63 |
| Subjects: | |
| Online Access: | Volltext |
| Item Description: | The book deals with the theory and practice of all electrophoretic steps leading to proteome analysis, i.e. isoelectric focusing (including immobilized pH gradients), sodium dodecyl sulphate electrophoresis (SADS-PAGE) and finally two-dimensional maps. It is a reasoned collection of all modern, relevant, up-to-date methodologies leading to successful fractionation, analysis and characterization of every polypeptide spot in 2-D map analysis. It includes chapters on the most sophisticated mass spectrometry developments and it helps the reader in navigating through the most important databases in proteome analysis, including step by step tours in selected sites. Yet, this book's unique strength and feature is the fact that it combines not only practice (in common with any other book on this topic) but also theory, by giving a detailed treatment on the most advanced theoretical treatments of steady-state techniques, such as isoelectric focusing and immobilized pH gradients. A lot of this theory is newly developed and presented to the public for the first time. Thus, this book should satisfy not only the needs of every day practitioners, but also the desires of the most advanced theoreticians in the field, who will surely appreciate the novel theories presented here. Also the methodological section contains several as yet unpublished protocols, correcting some of the existing ones and showing the pitfall and limitations of even well ingrained protocols in proteome analysis, which are here critically re-evaluated for the first time Includes bibliographical references and index |
| Physical Description: | 1 Online-Ressource (xiv, 395 p.) |
| ISBN: | 9780444505262 0444505261 9780080518961 0080518966 |
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| dewey-full | 572/.6 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 572 - Biochemistry |
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| discipline | Biologie |
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| illustrated | Not Illustrated |
| indexdate | 2024-07-10T01:18:10Z |
| institution | BVB |
| isbn | 9780444505262 0444505261 9780080518961 0080518966 |
| language | English |
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| series2 | Journal of chromatography library |
| spelling | Righetti, P. G. Verfasser aut The proteome revisited theory and practice of all relevant electrophoretic steps Pier Giorgio Righetti, Alexander Stoyanov, Mikhail Y. Zhukov 1st ed Amsterdam Elsevier Science 2001 1 Online-Ressource (xiv, 395 p.) txt rdacontent c rdamedia cr rdacarrier Journal of chromatography library v. 63 The book deals with the theory and practice of all electrophoretic steps leading to proteome analysis, i.e. isoelectric focusing (including immobilized pH gradients), sodium dodecyl sulphate electrophoresis (SADS-PAGE) and finally two-dimensional maps. It is a reasoned collection of all modern, relevant, up-to-date methodologies leading to successful fractionation, analysis and characterization of every polypeptide spot in 2-D map analysis. It includes chapters on the most sophisticated mass spectrometry developments and it helps the reader in navigating through the most important databases in proteome analysis, including step by step tours in selected sites. Yet, this book's unique strength and feature is the fact that it combines not only practice (in common with any other book on this topic) but also theory, by giving a detailed treatment on the most advanced theoretical treatments of steady-state techniques, such as isoelectric focusing and immobilized pH gradients. A lot of this theory is newly developed and presented to the public for the first time. Thus, this book should satisfy not only the needs of every day practitioners, but also the desires of the most advanced theoreticians in the field, who will surely appreciate the novel theories presented here. Also the methodological section contains several as yet unpublished protocols, correcting some of the existing ones and showing the pitfall and limitations of even well ingrained protocols in proteome analysis, which are here critically re-evaluated for the first time Includes bibliographical references and index Proteome / isolation & purification / Laboratory Manuals Isoelectric Focusing / methods / Laboratory Manuals SCIENCE / Life Sciences / Biochemistry bisacsh Electrophoresis fast Proteins / Separation fast Proteins Separation Electrophoresis Proteom (DE-588)4576155-3 gnd rswk-swf Elektrophorese (DE-588)4014373-9 gnd rswk-swf Proteom (DE-588)4576155-3 s Elektrophorese (DE-588)4014373-9 s 1\p DE-604 Stoyanov, Alexander Sonstige oth Zhukov, M. I͡U. Sonstige oth http://www.sciencedirect.com/science/book/9780444505262 Verlag Volltext 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Righetti, P. G. The proteome revisited theory and practice of all relevant electrophoretic steps Proteome / isolation & purification / Laboratory Manuals Isoelectric Focusing / methods / Laboratory Manuals SCIENCE / Life Sciences / Biochemistry bisacsh Electrophoresis fast Proteins / Separation fast Proteins Separation Electrophoresis Proteom (DE-588)4576155-3 gnd Elektrophorese (DE-588)4014373-9 gnd |
| subject_GND | (DE-588)4576155-3 (DE-588)4014373-9 |
| title | The proteome revisited theory and practice of all relevant electrophoretic steps |
| title_auth | The proteome revisited theory and practice of all relevant electrophoretic steps |
| title_exact_search | The proteome revisited theory and practice of all relevant electrophoretic steps |
| title_full | The proteome revisited theory and practice of all relevant electrophoretic steps Pier Giorgio Righetti, Alexander Stoyanov, Mikhail Y. Zhukov |
| title_fullStr | The proteome revisited theory and practice of all relevant electrophoretic steps Pier Giorgio Righetti, Alexander Stoyanov, Mikhail Y. Zhukov |
| title_full_unstemmed | The proteome revisited theory and practice of all relevant electrophoretic steps Pier Giorgio Righetti, Alexander Stoyanov, Mikhail Y. Zhukov |
| title_short | The proteome revisited |
| title_sort | the proteome revisited theory and practice of all relevant electrophoretic steps |
| title_sub | theory and practice of all relevant electrophoretic steps |
| topic | Proteome / isolation & purification / Laboratory Manuals Isoelectric Focusing / methods / Laboratory Manuals SCIENCE / Life Sciences / Biochemistry bisacsh Electrophoresis fast Proteins / Separation fast Proteins Separation Electrophoresis Proteom (DE-588)4576155-3 gnd Elektrophorese (DE-588)4014373-9 gnd |
| topic_facet | Proteome / isolation & purification / Laboratory Manuals Isoelectric Focusing / methods / Laboratory Manuals SCIENCE / Life Sciences / Biochemistry Electrophoresis Proteins / Separation Proteins Separation Proteom Elektrophorese |
| url | http://www.sciencedirect.com/science/book/9780444505262 |
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