Mitochondrial function:
Saved in:
| Other Authors: | , |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
Amsterdam
Elsevier
2014
|
| Edition: | First edition |
| Series: | Methods in enzymology
Volume 547 |
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | XX, 486 Seiten, ungezählte Folge von Seiten Illustrationen |
| ISBN: | 9780128014158 |
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Record in the Search Index
| _version_ | 1804152835931111424 |
|---|---|
| adam_text | Titel: Mitochondrial function
Autor: Murphy, Anne N
Jahr: 2014
CONTENTS
Contributors xiii
Preface xix
1. High-Content Functional Genomic Screening to Identify Novel
Regulators of the PINK1-Parkin Pathway 1
Andy Cheuk-Him Ng, Stephen D. Baird, and Robert A. Screaton
1. Introduction and Theory 2
2. General Screen Design Strategy 3
3. RNAi Screen for Genes Involved Required for PARK2 Translocation 6
4. Equipment 8
5. Materials 8
6. Genome Screen Protocol 10
Acknowledgments 18
References 18
2. Measurement of Mitochondrial Turnover and Life Cycle
Using MitoTimer 21
Kyle M. Trudeau, Roberta A. Gottlieb, and Orian S. Shirihai
1. Introduction 22
2. Expressing MitoTimer in Cells 23
3. Imaging MitoTimer 28
4. Interpreting MitoTimer Readout 29
5. Summary 36
References 36
3. Monitoring Mitophagy in Mammalian Cells 39
Vushan Zhu, Guo Chen, Linbo Chen, Welling Zhang, Du Feng,
Lei Liu, and Quan Chen
1. Introduction 40
2. Transmission Electron Microscopy 40
3. Western Blot Analysis of Mitophagy 42
4. Fluorescence Methods for Analyzing Mitophagy 45
5. Analyzing Mitophagy Using Mitochondria Mass 47
6. Mitochondria-Targeting Probes for Mitophagy Assay 47
7. Mitophagy Inducer and Inhibitors 49
vi
Contents
8. Future Perspectives
Acknowledgments
References
4. Photoactivatable Green Fluorescent Protein-Based
Visualization and Quantification of Mitochondrial Fusion
and Mitochondrial Network Complexity in Living Cells
Mariusz Karbowski, Megan M. Cleland, and Brian A. Roelofs
1. Introduction
2. Visualization and Quantification of Mitochondrial Fusion in Living Cells
3. Estimation of Relative Size of Mitochondrial Units and Mitochondrial Network
Complexity
4. Time-Lapse Imaging of Mitochondria in Living Cells: General Considerations
5. Concluding Remarks: Other Potential Applications of Mito-PAGFP
Acknowledgments
References
5. Characterization of Mitochondrial Transport in Neurons
Bing Zhou, Mei-Yao Lin, Tao Sun, Adam L. Knight, and Zu-Hang Sheng
1. Introduction
2. Analyses of Mitochondrial Transport in Embryonic Neuron Cultures
3. Analyses of Mitochondrial Transport in Adult Neuron Cultures
4. Characterization of Mitochondrial Transport at Synapses
5. Summary
Acknowledgments
References
6. Imaging of Mitochondrial Dynamics in Motor and Sensory
Axons of Living Mice
Irene Bolea, Wen-Biao Gan, Giovanni Manfedi, and Jordi Magrané
1. Introduction
2. Protocols to Image Mitochondrial Transport in the Peripheral Nerves
of Living Mice
3. Imaging of Mitochondrial Dynamics in the Peripheral Nerves
of Living Mice
4. Conclusions
Acknowledgments
References
51
52
52
57
58
61
66
68
70
71
71
75
76
79
84
90
94
94
95
97
98
99
105
108
109
109
Contents
vii
7. Analyzing Mitochondrial Dynamics in Mouse Organotypic
Slice Cultures 111
Anh H. Pham and David C. Chan
1. Parasagittal Slice Cultures of the Basal Ganglia 112
2. Cerebellar Slice Cultures 118
3. Monitoring Mitochondrial Dynamics in Slice Cultures 121
References 127
8. Analysis of Mitochondrial Traffic in Drosophila 131
Swathi Devireddy, Hyun Sung, Pin-Chao Liao, Elisabeth Garland-Kuntz,
and Peter J. Hollenbeck
1. Introduction 132
2. Dissection Methods for Drosophila Larvae 133
3. Imaging 139
4. Analysis 141
5. Conclusions 148
Acknowledgment 148
References 149
9. In Vivo Imaging of Mitochondria in Intact Zebrafish Larvae 151
Dominik Paquet, Gabriela Plucinska, and Thomas Misgeld
1. Introduction 152
2. Preparation of Transgenic MitoFish for Live Imaging 153
3. Visualizing Axonal Transport of Mitochondria in Zebrafish Sensory Neurons 157
4. Processing and Quantification of Imaging Files 160
5. Conclusions 162
Acknowledgments 162
References 163
10. The Use of miniSOG in the Localization of Mitochondrial
Proteins 165
Guy A. Perkins
1. Introduction 166
2. Requirements for CLEM Labeling 166
3. miniSOG Features 166
4. Resolution 168
5. Photooxidation Protocol for a Monolayer of Cultured Cells 169
6. Photooxidation Protocol for Tissues 174
viii Contents
7. Example of miniSOG Use with MCU 175
8. Conclusions and Future Work 175
Acknowledgments 178
References 178
11. Assessing the Function of Mitochondria-Associated
ER Membranes 181
Estela Area-Gomez
1. Introduction 181
2. Isolation of MAM 183
3. Assaying MAM Activity 185
References 195
12. Measurement of ROS Homeostasis in Isolated Mitochondria 199
L. Fretter and A. Ambrus
1. Importance of Quantifying Mitochondrial ROS Flomeostasis 200
2. Detection of Mitochondrial ROS Formation 205
3. Mitochondrial Elimination of Fl202 215
4. Methods for the Detection of Oxidative Stress 217
Acknowledgments 219
References 219
13. Use of Potentiometrie Fluorophores in the Measurement
of Mitochondrial Reactive Oxygen Species 225
Brian M. Polster, David G. Nicholls, Shealinna X. Ge, and Brian A. Roelofs
1. Introduction
2. ROS Detection Using MitoSOX—Subcellular Localization and
Fluorescence Yield
3. Validating MitoSOX Using a Negative Control
4. Choosing a Correct MitoSOX Loading Paradigm—Additional
Considerations
5. Is MitoSOX Imaging Useful?
6. Preparation of Primary Rat Cortical Neurons for Imaging
7. Optimizing MitoSOX Concentration and Establishing Mitochondrial
Localization 245
8. Imaging Using MitoSOX 247
Acknowledgment 247
References 247
226
228
235
237
240
244
Contents
ix
14. Spatial, Temporal, and Quantitative Manipulation of Intracellular
Hydrogen Peroxide in Cultured Cells 251
Ishraq Alim, Renee E. Haskew-Layton, Hossein Aleyasin, Hengchang Guo,
and Rajiv R. Ratan
1. Introduction 252
2. Production of H202 Using DAAO 254
3. Two-Photon Fluorescence Imaging of H202 265
4. Summary 269
Acknowledgments 270
References 270
15. Biochemical and Biophysical Methods for Studying
Mitochondrial Iron Metabolism 275
Gregory P. Holmes-Hampton, Wing-Hang Tong, and Tracey A. Rouault
1. Introduction 276
2. Measurement of Total Iron Concentration 277
3. In Situ Analysis of Iron in the Mitochondria 282
4. Biophysical Methods for Studying Iron in Isolated Mitochondria 290
5. Conclusions 299
Acknowledgments 299
References 299
16. Analysis and Interpretation of Microplate-Based Oxygen
Consumption and pH Data 309
Ajit S. Divakaruni, Alexander Paradyse, David A. Ferrick,
Anne N. Murphy, and Martin Jastroch
1. Introduction 310
2. The Oxygen Consumption Rate 311
3. The Extracellular Acidification Rate 330
Summary 347
Acknowledgments 347
References 347
17. Imaging Changes in the Cytosolic ATP-to-ADP Ratio 355
Mathew Tantama and Gary Vellen
1. Introduction 356
2. Methods 361
3. Expected Results 366
X
Contents
4. Summary 369
References 369
18. The Use of Mitochondria-Targeted Endonucieases
to Manipulate mtDNA 373
Sandra R. Bacman, Sion L. Williams, Milena Pinto, and Carlos T. Moraes
1. Mitochondrial DNA 374
2. Mitochondria-Targeted Restriction Nucleases to Cleave mtDNA
and Model OXPHOS Diseases 375
3. mtDNA Heteroplasmy and Approaches to Alter the Balance
Between Wild-Type and Mutant mtDNA 377
4. mtDNA Heteroplasmy Shift Using Restriction Endonucieases 379
5. Designer Endonucieases for the Modulation of mtDNA Heteroplasmy 382
6. mtDNA Heteroplasmy Shift Using Zinc-Finger Nucleases 384
7. Heteroplasmy Shift Using TAL Effector Nucleases 384
8. Single-Strand Annealing Assay to Analyze the Efficacy and
Specificity of Designer Nuclease 387
9. The Use of Cybrid Cells to Test Approaches to Change
Mitochondrial DNA Heteroplasmy 388
10. Immunodetection and Mitochondrial Localization in Cells
and Tissues 389
11. Changing mtDNA Heteroplasmy in Cultured Cells with mito-TALENs 390
12. Evaluation of the mtDNA Content 392
13. Future Perspectives 393
Acknowledgments 393
References 394
19. Induced Pluripotent Stem Cell-Derived Models for
mtDNA Diseases 399
Riikka H. Hämäläinen
1. Introduction 400
2. Induced Pluripotent Stem Cells 400
3. Mitochondrial Disease 402
4. Conclusion 407
5. Generation of iPSCs from mtDNA Disease Patients 408
Acknowledgment 411
References 411
Contents xi
20. The Use of 18F-BCPP-EF as a PET Probe for Complex I
Activity in the Brain 417
Hideo Tsukada
1. Introduction 418
2. Design and Assessment of Candidate Compounds for MC-I Imaging Probes 419
3. Application for Stroke/lschemic Damage Imaging 421
4. Application for Imaging of Aging Effects on MC-I Activity 425
5. Conclusion 429
References 430
21. MR OEF Imaging in MELAS 433
Sheng Xie
1. Introduction of Magnetic Resonance Imaging Techniques 434
2. Significance of OEF Imaging in MELAS 437
3. Cerebral OEF Changes in Our Study of MELAS 439
References 443
Author Index
Subject Index
445
479
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| spelling | Mitochondrial function edited by Anne N. Murphy and David C. Chan First edition Amsterdam Elsevier 2014 XX, 486 Seiten, ungezählte Folge von Seiten Illustrationen txt rdacontent n rdamedia nc rdacarrier Methods in enzymology Volume 547 Mitochondrium (DE-588)4124939-2 gnd rswk-swf Chondriom (DE-588)4010043-1 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Mitochondrium (DE-588)4124939-2 s DE-604 Chondriom (DE-588)4010043-1 s 1\p DE-604 Murphy, Anne N. edt Chan, David C. (DE-588)1069421871 edt Methods in enzymology Volume 547 (DE-604)BV000000938 547 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027712202&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Mitochondrial function Methods in enzymology Mitochondrium (DE-588)4124939-2 gnd Chondriom (DE-588)4010043-1 gnd |
| subject_GND | (DE-588)4124939-2 (DE-588)4010043-1 (DE-588)4143413-4 |
| title | Mitochondrial function |
| title_auth | Mitochondrial function |
| title_exact_search | Mitochondrial function |
| title_full | Mitochondrial function edited by Anne N. Murphy and David C. Chan |
| title_fullStr | Mitochondrial function edited by Anne N. Murphy and David C. Chan |
| title_full_unstemmed | Mitochondrial function edited by Anne N. Murphy and David C. Chan |
| title_short | Mitochondrial function |
| title_sort | mitochondrial function |
| topic | Mitochondrium (DE-588)4124939-2 gnd Chondriom (DE-588)4010043-1 gnd |
| topic_facet | Mitochondrium Chondriom Aufsatzsammlung |
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