Verfügbarkeit: Correlative light and electron microscopy

Correlative light and electron microscopy: [1]

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Weitere Verfasser:	Verkade, Paul (HerausgeberIn)
Format:	Buch
Sprache:	English
Veröffentlicht:	Amsterdam [u.a.] Elsevier, Acad. Press 2012
Ausgabe:	1. ed.
Schriftenreihe:	Methods in cell biology 111
Online-Zugang:	Inhaltsverzeichnis Inhaltsverzeichnis
Beschreibung:	XIX, 404 S. [20] Bl. Ill., graph. Darst., [19] Bl.
ISBN:	9780124160262

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adam_text	CONTENTS Contributors xi Preface xvii 1. Imaging Fluorescently Labeled Complexes by Means of Multidimensional Correlative Light and Transmission Hlectron Microscopy: Practical Considerations K. Kobayashi, D. Cheng, M. Huynh, K.R. Ratimic, P. Thtmlamm andF. Braet Abstract 2 Í. introduction 2 II. Rationale 5 III. Methods 7 ÍV. instrumentation and Materials 13 V. Discussion 15 Acknowledgments 18 References 18 2. Visualizing Live Dynamics and Ultrastructure of Intracellular Organelies with Preembedding Correlative Light-Electron Microscopy Roman S. Polishchuk. Elena V, Polishchuk and Alhcrta Lumi Abstract 21 I. Introduction and Rationale 22 (I. Materials 24 Ml. Methods 27 IV. Discussion . 32 V. Summary 34 Acknowledgments 34 References 34 3. Correlative Fluorescence and Transmission Electron Microscopy iti Tissues Toshihiro Takizawa and, lohn M. Robinson Abstract 37 I. Introduction 38 II. Correlative Microscopy: Reporter Systems 39 VI Contents III. Correlative Microscopy of Tissues 44 IV. Conclusions 50 References 52 4. Correlative Light and Electron Microscopy in Parasite Research Celine Loussert, Claire-Lise Forestier and Bruno M. Humbel Abstract 59 1. Introduction 60 II. Rationale 61 III. Methods 63 IV. Experiments and Materials 65 V. Results and Discussion 67 VI. Summary 71 Acknowledgments 72 References 72 5. Labeling of Ultrathin Resin Sections for Correlative Light and Electron Microscopy Gunar Fabig, Susanne Kretschmar, Susanne Weiche, Dominic Eberle. Marins Ader and Thomas Kurth Abstract 75 I. Introduction 76 П. Rationale 77 III. Methods 77 IV. Materials 88 V. Summary and Outlook 89 Acknowledgments 90 References 90 6. 3D HDO-CLEM: Cellular Compartment Analysis by Correlative Light-Electron 4icroscopy on Cryosection Kalkt Cortese, Giuseppe Vkìdomìni, Maria Cristina Gagiìam, Patrizia Boccacci, Alberto Diaspro and Carlo Tacchetti Abstract 96 1. Introduction 96 H. Materials 9S HI. Methods 100 IV. Notes Ilo Acknowledgments 114 References 1 з 4 Contents vii 7. Correlative Light and Electron Microscopy of GFP Markus Grabenhauer Abstract 117 1. Introduction 118 II. Rationale 124 III. Methods 125 IV. Materials 128 V. Discussion 129 Acknowledgments 135 References 135 8. Picking Paces out of a Crowd: Genetic Labels lor Identification of Proteins in Correlated Light and Electron Microscopy Imaging Mark H. Ellisman, Thomas J. Deerinck, Xkiokun Shu and (ìimi E. Sosinsky Abstract 140 I. Introduction í 40 II. The Oowded Cell and Spatiotemporal Proteomks 140 III. What EM Has to Offer 14] ÍV. Immunomarkers 142 V. Genetically Appended or Inserted Protein Tags 143 VI. Types of Genetic Tags Currently Available 144 VII. Future Directions and Challenges 152 Acknowledgments 153 References 153 9. Correlated Light Microscopy and Election Microscopy Klaas A. Sjtillema, Ulrike Schnell Jemen Kuipers, Ruby Kalichanm and Ben S.O. Giepmaiis Abstract 157 Abbreviations 158 I. Introduction 158 II. Correlated Light and Electron Microscopy: Using the Best of Two Worlds 159 III. ROls: Search & Find Tools 161 IV. Our Approach: Virtual Reality Overlay During Preparation 165 V. Discussion and Conclusion 169 VI. Future Perspective 170 Acknowledgments 171 References 171 viii Contents 10. Capturing Endocytic Segregation Events with HPF-CLLM Edward Brown, Jan van Weering, Thorn Sharp, Judith Munteli, and Paul Verkade Abstract 176 I. Introduction 176 II. Methods 181 III. Outlook 198 Acknowledgments 199 References 199 11. Targeted Ultramicrotomy: A Valuable Tool for Correlated Light and Electron Microscopy of Small Model Organisms Irina Kolotuev, DanielJ. Bumbarger, Michel Labouesse and Yannick Schwab Abstract 204 I. Introduction 204 II. Rationale 205 III. Methods 206 IV. Instrumentation and Materials 216 V. Discussion 219 Acknowledgments 221 References 221 12. Correlative Light and Electron Microscopy of Intermediate Stages of Meiotic Spindle Assembly in the Early Caenorhabditis elegans Embryo Ina Woog, Silke White, Mandy Büchner, Martin Srayko and Thomas Müller-Reichert Abstract 224 1. Introduction 224 IL. Methods 226 III. Instrumentation and Material 230 IV. Discussion 231 Acknowledgments 233 References 233 13. Precise, Correlated Fluorescence Microscopy and Electron Tomography of Lowicryl Sections Using Fluorescent Fiducial Markers Wanda Kukulski, Martin Schorb, Sonja Welsch, Andrea Picco, Marko Kaksonen and John A.G. Briggs Abstract 236 I. Introduction 236 II. Rationale 239 Contents ix III. Methods 239 IV. Instrumentation and Materials 248 V. Discussion 250 References 255 14. Integrative Approaches for Cellular Cryo-electron Tomography: Correlative Imaging and Focused Ion Beam Micromachining Alexander Rigori, Elizabeth Villa, Felix J.B. Bäuerlein, Benjamin D. Engel and Jürgen M. Plitzko Abstract 259 I. Introduction 260 II. Rationale 262 III. Methods 263 IV. Instrumentation and Materials 275 V. Discussion and Outlook 277 Acknowledgments 278 References 279 15. Visualizing Proteins in Electron Micrographs at Nanometer Resolution Shigeki Watanahe and Erik M. Jorgensen Abstract 284 I. Introduction 284 11. Rationale 286 III. Methods 287 IV. Instrumentations and Materials 298 V. Discussion 301 VI. Perspective 304 Acknowledgments 304 References 304 16. Atmospheric Scanning Electron Microscope for Correlative Microscopy Ian E.G. Mormon, Clare L. Dennison, HidetoshiNishiyama, Mitsuo Suga, Chikara Sato, Andrew Yanvood and Peter J. O Took Abstract 307 1. Introduction: Atmospheric Scanning Electron Microscopy 308 И. Design of the JEOUASM^OO 309 III. Sample Preparation 311 IV. Application Studies 315 V. Discussion 321 Acknowledgments 323 References 323 Contents 17. Bridging Microscopes: 3D Correlative Light and Scanning Electron Microscopy of Complex Biological Structures Miriam S. Lucas, Maja Güníhert, Philippe Gasser, Falk Lucas and Roger Wepf Abstract 326 I. Introduction 326 II. Rationale 330 ΠΙ. Methods 331 IV. Materials and Instrumentation 348 V. Discussion 350 VI. Summary 352 Acknowledgments 353 References 353 18. Conelative Light and Volume Electron Microscopy: Using Focused Ion Beam Scanning Electron Microscopy to Image Transient Events in Model Organisms Andrew J. Bushby, Giovanni Mariggi, Hannah E. J. Armer and Lucy M. Collinson Abstract 358 I. Introduction 358 II. Rationale 359 III. Materials 360 IV. Methods 361 V. Discussion 378 VI. Summary 380 Acknowledgments 380 References 380 Index 383 Volumes in Series 395 CONTENTS Contributors xi Preface xvii 1. Imaging Fluorescently Labeled Complexes by Means of Multidimensional Correlative Light and Transmission Electron Microscopy: Practical Considerations K. Kobayashi, D. Cheng, M. Huynh, K.R. Ratinac, P. Thordarson and F. Brae t Abstract 2 I. Introduction 2 II. Rationale 5 HI. Methods 7 IV. Instrumentation and Materials 13 V. Discussion 15 Acknowledgments 18 References 18 2. Visualizing Live Dynamics and Ultrastructure of Intracellular Organelles with Preembedding Correlative Light-Electron Microscopy Roman S. Polishchuk, Elena V. Polishchuk and Alberto Lu ini Abstract 21 I. Introduction and Rationale 22 II. Materials 24 III. Methods 27 IV. Discussion 32 V. Summary 34 Acknowledgments 34 References 34 3. Correlative Fluorescence and Transmission Electron Microscopy in Tissues Toshihiro Takizawa and John M. Robinson Abstract 37 I. Introduction 38 II. Correlative Microscopy: Reporter Systems 39 Contents III. Correlative Microscopy of Tissues 44 IV. Conclusions 50 References 52 4. Correlative Light and Electron Microscopy in Parasite Research Céline Loussert, Claire-Lise Forestier and Bruno M. Humbel Abstract 59 I. Introduction 60 II. Rationale 61 III. Methods 63 IV. Experiments and Materials 65 V. Results and Discussion 67 VI. Summary 71 Acknowledgments 72 References 72 5. Labeling of Ultrathin Resin Sections for Correlative Light and Electron Microscopy Gunar Fabig, Susanne Kretschmar, Susanne Weiche, Dominic Eberle, Marius Ader and Thomas Kurth Abstract 75 I. Introduction 76 II. Rationale 77 III. Methods 77 IV. Materials 88 V. Summary and Outlook 89 Acknowledgments 90 References 90 6. 3D HDO-CLEM: Cellular Compartment Analysis by Correlative Light-Electron Microscopy on Cryosection Katia Cortese, Giuseppe Vicidomini, Maria Cristina Gagliani, Patrizia Boccacci, Alberto Diaspro and Carlo Tacchetti Abstract 96 I. Introduction 96 II. Materials 98 HI. Methods 100 IV. Notes і 10 Acknowledgments 114 References 114 Contents 7. Correlative Light and Electron Microscopy of GFP Markus Grabenbauer Abstract 117 I. Introduction 118 II. Rationale 124 III. Methods 125 IV. Materials 128 V. Discussion 129 Acknowledgments 135 References 135 8. Picking Faces out of a Crowd: Genetic Labels for Identification of Proteins in Correlated Light and Electron Microscopy Imaging Mark H. Ellisman, Thomas J. Deerinck, Xiaokun Shu and Gina E. Sosinsky Abstract 140 I. Introduction 140 II. The Crowded Cell and Spatiotemporal Proteomics 140 III. What EM Has to Offer 141 IV. Immunomarkers 142 V. Genetically Appended or Inserted Protein Tags 143 VI. Types of Genetic Tags Currently Available 144 VII. Future Directions and Challenges 152 Acknowledgments 153 References 153 9. Correlated Light Microscopy and Electron Microscopy Klaas A. Sjollema, Ulrike Schnell, Jemen Kuipers, Ruby Kalicharan and Ben N. G. Gìepmans Abstract 157 Abbreviations 158 I. Introduction 158 II. Correlated Light and Electron Microscopy: Using the Best of Two Worlds 159 III. ROIs: Search & Find Tools 161 IV. Our Approach: Virtual Reality Overlay During Preparation 165 V. Discussion and Conclusion 169 VI. Future Perspective 170 Acknowledgments 171 References 171 viii Contents 10. Capturing Endocytic Segregation Events with HPF-CLEM Edward Brown, Jan van Weering, Thorn Sharp, Judith Mantell, and Paul Verkade Abstract 176 I. Introduction 176 II. Methods 181 III. Outlook 198 Acknowledgments 199 References 199 11. Targeted Ultramicrotomy: A Valuable Tool for Correlated Light and Electron Microscopy of Small Model Organisms Irina Kolotuev, Daniel J. Bumbarger, Michel Labouesse and Yannick Schwab Abstract 204 I. Introduction 204 II. Rationale 205 III. Methods 206 IV. Instrumentation and Materials 216 V. Discussion 219 Acknowledgments 221 References 221 12. Correlative Light and Electron Microscopy of Intermediate Stages of Meiotic Spindle Assembly in the Early Caenorhabditis elegans Embryo Ina Woog, Silke White, Mandy Büchner, Martin Srayko and Thomas Müller-Reichert Abstract 224 I. Introduction 224 II. Methods 226 III. Instrumentation and Material 230 IV. Discussion 231 Acknowledgments 233 References 233 13. Precise, Correlated Fluorescence Microscopy and Electron Tomography of Lowicryl Sections Using Fluorescent Fiducial Markers Wanda Kukulski, Martin Schorb, Sonja Welsch, Andrea Picco, Marko Kaksonen and John A.G. Briggs Abstract 236 I. Introduction 236 II. Rationale 239 Contents III. Methods 239 IV. Instrumentation and Materials 248 V. Discussion 250 References 255 14. Integrative Approaches for Cellular Cryo-electron Tomography: Correlative Imaging and Focused Ion Beam Micromachining Alexander Rigori, Elizabeth Villa, Felix J.B. Bäuerlein, Benjamin D. Engel and Jürgen M. Plitzko Abstract 259 I. Introduction 260 II. Rationale 262 III. Methods 263 IV. Instrumentation and Materials 275 V. Discussion and Outlook 277 Acknowledgments 278 References 279 15. Visualizing Proteins in Electron Micrographs at Nanometer Resolution Shigeki Watanabe and Erik M. Jorgensen Abstract 284 I. Introduction 284 II. Rationale 286 III. Methods 287 IV. Instrumentations and Materials 298 V. Discussion 301 VI. Perspective 304 Acknowledgments 304 References 304 16. Atmospheric Scanning Electron Microscope for Correlative Microscopy Ian E.G. Morrison, Clare L. Dennison, Hidetoshi Nishiyama, Mitsuo Suga, Chikara Sato, Andrew Yarwood and Peter J. O Toóle Abstract 307 I. Introduction: Atmospheric Scanning Electron Microscopy 308 II. DesignoftheJEOLJASM-6200 309 III. Sample Preparation 311 IV. Application Studies 315 V. Discussion 321 Acknowledgments 323 References 323 Contents 17. Bridging Microscopes: 3D Correlative Light and Scanning Electron Microscopy of Complex Biological Structures Miriam S. Lucas, Maja Günthert, Philippe Gasser, Falk Lucas and Roger Wepf Abstract 326 I. Introduction 326 II. Rationale 330 III. Methods 331 IV. Materials and Instrumentation 348 V. Discussion 350 VI. Summary 352 Acknowledgments 353 References 353 18. Correlative Light and Volume Electron Microscopy: Using Focused Ion Beam Scanning Electron Microscopy to Image Transient Events in Model Organisms Andrew J. Bushby, Giovanni Mariggi, Hannah E. J. Armer and Lucy M. Collinson Abstract 358 I. Introduction 358 II. Rationale 359 III. Materials 360 IV. Methods 361 V. Discussion 378 VI. Summary 380 Acknowledgments 380 References 380 Index 383 Volumes in Series 395
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spelling	Correlative light and electron microscopy [1] ed. by Thomas Müller-Reichert, Paul Verkade 1. ed. Amsterdam [u.a.] Elsevier, Acad. Press 2012 XIX, 404 S. [20] Bl. Ill., graph. Darst., [19] Bl. txt rdacontent n rdamedia nc rdacarrier Methods in cell biology 111 Methods in cell biology Müller-Reichert, Thomas 1962- Sonstige (DE-588)132464020 oth Verkade, Paul (DE-588)113549391X edt (DE-604)BV042194467 1 Methods in cell biology 111 (DE-604)BV002534878 111 Digitalisierung UB Regensburg - ADAM Catalogue Enrichment application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027633691&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027633691&sequence=000004&line_number=0002&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis
spellingShingle	Correlative light and electron microscopy Methods in cell biology
title	Correlative light and electron microscopy
title_auth	Correlative light and electron microscopy
title_exact_search	Correlative light and electron microscopy
title_full	Correlative light and electron microscopy [1] ed. by Thomas Müller-Reichert, Paul Verkade
title_fullStr	Correlative light and electron microscopy [1] ed. by Thomas Müller-Reichert, Paul Verkade
title_full_unstemmed	Correlative light and electron microscopy [1] ed. by Thomas Müller-Reichert, Paul Verkade
title_short	Correlative light and electron microscopy
title_sort	correlative light and electron microscopy
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