Correlative light and electron microscopy: 2
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| Other Authors: | |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
Amsterdam [u.a.]
Elsevier, Acad. Press
2014
|
| Edition: | 1. ed. |
| Series: | Methods in cell biology
124 |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | XVIII, 442 S., [16] Bl. Ill., graph. Darst. |
| ISBN: | 9780128010754 |
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MARC
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| 245 | 1 | 0 | |a Correlative light and electron microscopy |n 2 |c ed. by Thomas Müller-Reichert, Paul Verkade |
| 250 | |a 1. ed. | ||
| 264 | 1 | |a Amsterdam [u.a.] |b Elsevier, Acad. Press |c 2014 | |
| 300 | |a XVIII, 442 S., [16] Bl. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Methods in cell biology |v 124 | |
| 490 | 0 | |a Methods in cell biology | |
| 700 | 1 | |a Müller-Reichert, Thomas |d 1962- |e Sonstige |0 (DE-588)132464020 |4 oth | |
| 700 | 1 | |a Verkade, Paul |0 (DE-588)113549391X |4 edt | |
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| 830 | 0 | |a Methods in cell biology |v 124 |w (DE-604)BV002534878 |9 124 | |
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| 999 | |a oai:aleph.bib-bvb.de:BVB01-027633506 | ||
Record in the Search Index
| _version_ | 1804152707462725632 |
|---|---|
| adam_text | Contents
Contributors
..............................................................................................................xi
Preface
..................................................................................................................xvii
CHAPTER
1
Retracing in Correlative Light Electron
Microscopy: Where is My Object of Interest?
................
ι
Lorna
Hodgson, David Nam, Judith Mantel!,
Alin
Achim,
Paul
Verkade
Introduction
.....................................................................................2
1.1
Steps in the CLEM Workflow
........................................................3
1.2
Methods
.........................................................................................
U
1.3
Materials
........................................................................................16
Conclusions
...................................................................................16
References
.....................................................................................18
CHAPTER
2
Fluorescing the Electron: Strategies
in Correlative Experimental Design
..................................23
Kimberley H. Gibson,
Daniela Vorkel,
Jana Meissner,
Jean-Marc Verbavatz
Introduction
...................................................................................24
2.1
Strategies for CLEM
.....................................................................32
2.2
Methods
.........................................................................................41
2.3
Materials
........................................................................................47
2.4
Discussion
.....................................................................................48
Acknowledgments
........................................................................52
References
.....................................................................................52
CHAPTER
3
Metallothioneins for Correlative Light
and Electron Microscopy
.....................................................55
Isabel
Fernández
de
Castro, Laura
Sanz-Sánchez,
Cristina Risco
Introduction...................................................................................
56
3.1
Rationale
.......................................................................................57
3.2
Methods
.........................................................................................58
3.3
Discussion
.....................................................................................65
Acknowledgments
........................................................................69
References
.....................................................................................69
vi
Contents
CHAPTER
4
Correlative Light Microscopy and Electron
Tomography to Study
Von Willebrand
Factor
Exocytosis from Vascular Endothelial Cells
..................71
Marjon
J. Mourík,
Frank G.A.
Faas,
Karine
M. Valentijn,
Jack A. Valentijn,
Jemen C.
Eikenboom,
Abraham
J.
Koster
Introduction
...................................................................................72
4.1
Rationale
.......................................................................................74
4.2
Methods
.........................................................................................75
4.3
Materials and Instrumentation
......................................................86
4.4
Discussion
.....................................................................................88
4.5
Outlook
..........................................................................................89
Acknowledgments
........................................................................90
References
.....................................................................................90
CHAPTER
5
A Simple Procedure to Analyze Positions
of Interest in Infectious Cell Cultures by
Correlative Light and Electron Microscopy
...................93
Kazimierz Madela,
Sebastian Banhart,
Anja Zimmermann,
Janett
Piesker,
Norbert Bannen, Michael Laue
Introduction and
Rationale...........................................................94
5.1
Methods
.........................................................................................96
5.2 Materials......................................................................................103
5.3
Discussion
...................................................................................105
Summary.....................................................................................
108
Acknowledgments
......................................................................108
References
...................................................................................108
CHAPTER
6
Correlative Light and Electron Microscopy
for a Free-Floating Spindle in Xenopus laevis
Egg Extracts
............................................................................
in
Erin M. Tranfield,
Xavier
Heiligenstein,
Ina Peristere,
Claude Antony
Introduction
.................................................................................112
6.1
Rationale
.....................................................................................113
6.2
Methods
.......................................................................................113
6.3
Perspectives and Future Directions
............................................126
Acknowledgments
......................................................................127
References
...................................................................................127
Contents
vii
CHAPTER
7
Combining Wide-Field Super-Resolution
Microscopy and Electron Tomography:
Rendering Nanoscopic Correlative Arrays on
Subcellular Architecture
....................................................129
Filip Braet, Delfine
Cheng, Minn Huynh, Jeffrey Henriquez,
Gerry Shami,
Marko
Lampe
Introduction
.................................................................................130
7.1
Rationale
.....................................................................................131
7.2
Methods
.......................................................................................132
7.3
Instrumentation and Materials
....................................................140
7.4
Results and Discussion
...............................................................142
Conclusion
...................................................................................146
Acknowledgments
......................................................................147
References
...................................................................................148
CHAPTER
8
Correlative Cryo-Fluorescence and Cryo-Soft
Х
-Ray Tomography of Adherent Cells at
European Synchrotrons
.......................................................
ш
Raffaella Carzaniga,
Marie-Charlotte Domati,
Elizabeth Duke,
Lucy M. Collinson
Introduction
.................................................................................152
8.1
Rationale
.....................................................................................153
8.2
Methods
.......................................................................................154
8.3
Materials
......................................................................................171
Outlook and Future Directions
...................................................175
Acknowledgments
.....................................................................,176
References
...................................................................................176
CHAPTER
9
Critical Step-by-Step Approaches Toward
Correlative Fluorescence/Soft X-Ray
Cryo-Microscopy of Adherent
Mammalian Cells
...................................................................179
Kyle C. Dent,
Christoph Hagen, Kay Grunewald
Introduction
.................................................................................181
9.1 Rationale.....................................................................................184
9.2
Critical Discussion of the Correlative
CryoFM/XT Workflow
...............................................................185
Conclusion and Prospects
...........................................................210
Acknowledgments
......................................................................211
References
...................................................................................211
viii Contents
CHAPTER
10
Correlative Cryo-Fluorescence Light Microscopy
and Cryo-Electron Tomography of Streptomyces
.......217
Roman I.
Koning,
Katherine
Celler,
Joost Willemse, Erik Bos,
Gilles P. van Wezel, Abraham J. Koster
Introduction
.................................................................................218
10.1
Rationale.....................................................................................
221
10.2
Methods
.......................................................................................221
10.3
Materials
and Instrumentation
....................................................233
10.4
Discussion and Outlook
..............................................................234
Acknowledgments
......................................................................236
References
...................................................................................236
CHAPTER
11
Immuno Correlative Light and Electron
Microscopy on Tokuyasu Cryosections
.........................241
Viola M.J.
Oorschot,
Tamar
E. Sztal,
Robert
J.
Bryson-Richardson, Georg Ramm
Introduction
.................................................................................242
11.1
Rationale
.....................................................................................244
11.2
Methods
.......................................................................................244
11.3
Materials
......................................................................................253
11.4
Instrumentation
...........................................................................255
11.5
Image Analysis Software
............................................................256
Acknowledgments
......................................................................256
References
...................................................................................256
CHAPTER
12
Step by Step Manipulation of the CryoCapsule
with HPM High Pressure Freezers
..................................259
Xavier
Heiligenstein,
lise Hurbain, Cédric Delevoye,
Jean
Salaméra,
Claude
Antony,
Graca
Raposo
Introduction
.................................................................................260
12.1
The CryoCapsule
........................................................................262
12.2
Combining the CryoCapsule with HPF/HPMs
..........................263
12.3
After HPF
....................................................................................267
12.4
Application to CLEM
.................................................................269
Conclusions
.................................................................................271
References
...................................................................................273
CHAPTER
13
Live-Cell CLEM of Subcellular Targets:
An Optimized Procedure for Polymer-Based
Imaging Substrates
...............................................................275
Benjamin S. Padman,
Georg Ramm
Introduction
.................................................................................276
13.1
Materials and Methods
...............................................................277
Contents ix
13.2
Demonstrative Example
..............................................................296
References
...................................................................................301
CHAPTER
14
Correlative Fluorescence and Scanning
Transmission Electron Microscopy of Quantum
Dot-Labeled Proteins on Whole Cells in Liquid
.........
305
Diana B. Peckys,
Vera Bandmann,
Niels
de Jonge
Introduction
.................................................................................306
14.1
Materials
....................................................................................307
14.2
Methods
......................................................................................310
14.3
Notes
...........................................................................................319
Acknowledgments
......................................................................321
References
...................................................................................321
CHAPTER
15
The Use of a Laser for Correlating Light and
Electron Microscopic Images in Thick Tissue
Specimens
...............................................................................323
Derron Bishop,
Ivana
Nikic, Martin Kerschensteiner,
Thomas
Misgeld
Introduction
.................................................................................324
15.1
General Features of NIRB
..........................................................325
15.2
Method
........................................................................................328
15.3
Materials
......................................................................................332
15.4
Discussion and Troubleshooting
.................................................334
Conclusions
.................................................................................336
References
...................................................................................336
CHAPTER
16
Correlative In Vivo 2-Photon Imaging and
Focused Ion Beam Scanning Electron Microscopy:
3D
Analysis of
Neuronal
Ultrastructure
........................
339
Bohumil
Масо,
Anthony Holtmaat, Anne Jorstad,
Pascal Fua,
Graham W.
Knott
Introduction
.................................................................................340
16.1
Method
........................................................................................342
Conclusion
...................................................................................359
Acknowledgments
......................................................................359
References
...................................................................................360
CHAPTER
17
Integrated Light and Scanning Electron
Microscopy of GFP-Expressing Cells
.............................
збз
Christopher J. Peddle, Nalan
Liv,
Jacob P. Hoogenboom,
Lucy M. Collinson
Introduction
.................................................................................364
17.1
Rationale
.....................................................................................365
Contents
17.2
Methods
.......................................................................................367
17.3 Materials......................................................................................384
17.4
Discussion
...................................................................................385
Acknowledgments
......................................................................386
References
...................................................................................387
CHAPTER
18
Correlation of the Same Fields Imaged in the
ТЕМ,
Confocal, LM, and MicroCT by Image Registration:
From Specimen Preparation to Displaying a Final
Composite Image
...................................................................391
Douglas R. Keene, Sara L. Tufa, Melissa H. Wong,
Nicholas R. Smith, Lynn
Y. Sakai, William
A.
Horton
Introduction
.................................................................................392
18.1
Methods
.......................................................................................399
References
...................................................................................414
Further Reading
..........................................................................417
Index
.......................................................................................................................419
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| language | English |
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| series | Methods in cell biology |
| series2 | Methods in cell biology |
| spelling | Correlative light and electron microscopy 2 ed. by Thomas Müller-Reichert, Paul Verkade 1. ed. Amsterdam [u.a.] Elsevier, Acad. Press 2014 XVIII, 442 S., [16] Bl. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in cell biology 124 Methods in cell biology Müller-Reichert, Thomas 1962- Sonstige (DE-588)132464020 oth Verkade, Paul (DE-588)113549391X edt (DE-604)BV042194467 2 Methods in cell biology 124 (DE-604)BV002534878 124 Digitalisierung UB Regensburg - ADAM Catalogue Enrichment application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027633506&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Correlative light and electron microscopy Methods in cell biology |
| title | Correlative light and electron microscopy |
| title_auth | Correlative light and electron microscopy |
| title_exact_search | Correlative light and electron microscopy |
| title_full | Correlative light and electron microscopy 2 ed. by Thomas Müller-Reichert, Paul Verkade |
| title_fullStr | Correlative light and electron microscopy 2 ed. by Thomas Müller-Reichert, Paul Verkade |
| title_full_unstemmed | Correlative light and electron microscopy 2 ed. by Thomas Müller-Reichert, Paul Verkade |
| title_short | Correlative light and electron microscopy |
| title_sort | correlative light and electron microscopy |
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