Single molecule microscopy: investigations of highly oriented mesoporous silica channels, photophysics of new fluorescent rylene derivatives and synchronous emission on silver nanowires
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| Format: | Thesis Book |
| Language: | English |
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2013
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| Subjects: | |
| Online Access: | Volltext kostenfrei Inhaltsverzeichnis |
| Physical Description: | XIII, 133 S. Ill., graph. Darst. CD-ROM (12 cm) |
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| _version_ | 1804152470222405632 |
|---|---|
| adam_text | DISSERTATION
ZUR ERLANGUNG
DES DOKTORGRADES
DER FAKULTAT
CHEMIE UND PHARMAZIE
DER LUDWIG-MAXIMILIANS-UNIVERSITAT
MUNCHEN
SINGLE MOLECULE MICROSCOPY
INVESTIGATIONS OF HIGHLY ORIENTED MESOPOROUS SILICA CHANNELS,
PHOTOPHYSICS OF NEW FLUORESCENT RYLENE DERIVATIVES
AND
SYNCHRONOUS EMISSION ON SILVER NANOWIRES
EVA MELARI DAVIES
AUS
MIINCHEN, DEUTSCHLAND
2013
HTTP://D-NB.INFO/1058560654
CONTENTS
SUMMARY V
1 INTRODUCTION 1
2 SINGLE-MOLECULE SPECTROSCOPY (SMS) 5
2.1 FLUORESCENCE 5
2.2 PRINCIPLES AND TECHNIQUES 8
2.2.1 WIDE-FIELD FLUORESCENCE MICROSCOPY 8
2.2.2 LASER SCANNING CONFOCAL MICROSCOPY (LSCM) 11
2.3 SINGLE-MOLECULE TRACKING (SMT) 12
2.4 DIFFUSION THEORY 14
3 NEW PHOTOSTABLE RYLENE DERIVATIVES FOR SINGLE-MOLECULE STUDIES 19
3.1 INTRODUCTION: HIGHLY FLUORESCENT WATER-SOLUBLE DYES 19
3.2 PHOTOPHYSICS OF NEW PHOTOSTABLE RYLENE DERIVATIVES 21
3.2.1 ABSORPTION AND EMISSION SPECTRA 22
3.2.2 SINGLE-MOLECULE STUDIES 2-5
3.2.3 MEMBRANE LABELING 27
3.2.4 CONCLUSION 28
3.2.5 EXPERIMENTAL PART 28
3.3 ORTHO-FUNCTIONALIZED PERYLENEDIIMIDES FOR HIGHLY FLUORESCENT
WATER-SOLUBLE DYES . 32
3.3.1 SYNTHETIC ROUTE OF ORTHO-WS-PDI 33
3.3.2 ABSORPTION AND EMISSION SPECTRA 33
3.3.3 SINGLE-MOLECULE STUDIES 34
3.3.4 INTERACTION OF ORTHO-WS-PDI WITH HELA CELLS 35
3.3.5 CONCLUSION 36
3.3.6 EXPERIMENTAL PART 36
4 HIGHLY ORIENTED MESOPOROUS SILICA CHANNELS FOR SINGLE-MOLECULE STUDIES
39
4.1 INTRODUCTION: MESOPOROUS SILICA MATERIALS 40
4.1.1 SYNTHESIS STRATEGIES AND FORMATION MECHANISM 40
4.1.2 THIN FILM DEPOSITION AND FUNCTIONALIZATION TECHNIQUES 44
4.1.3 CHARACTERIZATION OF HOST-GUEST INTERACTIONS 47
4.2 HIGHLY ORIENTED MESOPOROUS SILICA CHANNELS SYNTHESIZED IN
MICROGROOVES 49
4.2.1 CHARACTERIZATION TECHNIQUES 50
4.2.2 EXPERIMENTAL CONDITIONS 51
4.2.3 DETAILED ANALYSIS OF TRAJECTORIES 57
4.2.4 STATISTICAL ANALYSIS 61
XI
CONTENTS
4.2.5 LENGTH OF THE ACCESSIBLE PORES 62
4.2.6 EXTRACTED MESOPORES 63
4.2.7 CONCLUSION 65
4.2.8 EXPERIMENTAL PART 65
4.3 ELECTROPHORESIS OF SINGLE DYE MOLECULES IN HIGHLY ORIENTED
MESOPOROUS SILICA CHANNELS 69
4.3.1 DIFFUSIONAL BEHAVIOR OF DIP-TDI, AS-TDI AND SW-TDI 71
4.3.2 DIFFUSIONAL BEHAVIOR OF AMMONIUM-TDI 74
4.3.3 DIFFUSIONAL BEHAVIOR OF PHPY-PDI VERSUS AS-TDI 74
4.3.4 DIFFUSIONAL BEHAVIOR OF ATTO633 VERSUS AS-TDI 76
4.3.5 MODEL OF THE HOST-GUEST SYSTEM 79
4.3.6 AVERAGE VELOCITY 80
4.3.7 ELECTROPHORETIC SEPARATION OF WS-TDI AND PY-PDI 81
4.3.8 CONCLUSION 82
4.3.9 EXPERIMENTAL PART 83
4.4 ELECTROPHORESIS OF DNA IN HIGHLY ORIENTED MESOPOROUS SILICA CHANNELS
85
4.4.1 INTRODUCTION 85
4.4.2 STRATEGY TO INCORPORATE DNA INTO TEMPLATE-FILLED MESOPORES 86
4.4.3 STRATEGY TO INCORPORATE DNA INTO EXTRACTED MESOPORES 87
4.4.4 CONCLUSION 88
4.4.5 EXPERIMENTAL PART 89
5 OPTICAL AND STRUCTURAL PROPERTIES OF SILVER NANOWIRES 91
5.1 INTRODUCTION: PLASMONICS IN METALS 91
5.2 SYNCHRONOUS EMISSION ON SILVER NANOWIRES 93
5.2.1 TRANSMISSION ELECTRON MICROSCOPY (TEM) STUDIES 93
5.2.2 WIDE-FIELD FLUORESCENCE STUDIES 96
5.2.3 CORRELATION OF OPTICAL AND CHEMICAL PROPERTIES 103
5.2.4 CONCLUSIONS 105
5.2.5 EXPERIMENTAL PART 105
6 APPENDIX 107
6.1 ORTHO-FUNCTIONALIZED PERYLENEDIIMIDES VERSUS FLUORESCEIN 107
6.1.1 SINGLE-MOLECULE STUDIES 107
6.1.2 CONCLUSION 108
6.1.3 EXPERIMENTAL PART 109
LIST OF ABBREVIATIONS 109
BIBLIOGRAPHY 113
ACKNOWLEDGEMENTS 129
XII
LIST OF PUBLICATIONS
CURRICULUM VITAE
CONTENTS
131
133
XIII
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| spelling | Davies, Eva Melari 1985- Verfasser (DE-588)1056898909 aut Single molecule microscopy investigations of highly oriented mesoporous silica channels, photophysics of new fluorescent rylene derivatives and synchronous emission on silver nanowires Eva Melari Davies 2013 XIII, 133 S. Ill., graph. Darst. CD-ROM (12 cm) txt rdacontent n rdamedia nc rdacarrier München, Univ., Diss., 2013 (DE-588)4113937-9 Hochschulschrift gnd-content Erscheint auch als Online-Ausgabe urn:nbn:de:bvb:19-173355 http://edoc.ub.uni-muenchen.de/17335/ Verlag kostenfrei Volltext https://nbn-resolving.org/urn:nbn:de:bvb:19-173355 Resolving-System Verlag kostenfrei DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027483861&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Davies, Eva Melari 1985- Single molecule microscopy investigations of highly oriented mesoporous silica channels, photophysics of new fluorescent rylene derivatives and synchronous emission on silver nanowires |
| subject_GND | (DE-588)4113937-9 |
| title | Single molecule microscopy investigations of highly oriented mesoporous silica channels, photophysics of new fluorescent rylene derivatives and synchronous emission on silver nanowires |
| title_auth | Single molecule microscopy investigations of highly oriented mesoporous silica channels, photophysics of new fluorescent rylene derivatives and synchronous emission on silver nanowires |
| title_exact_search | Single molecule microscopy investigations of highly oriented mesoporous silica channels, photophysics of new fluorescent rylene derivatives and synchronous emission on silver nanowires |
| title_full | Single molecule microscopy investigations of highly oriented mesoporous silica channels, photophysics of new fluorescent rylene derivatives and synchronous emission on silver nanowires Eva Melari Davies |
| title_fullStr | Single molecule microscopy investigations of highly oriented mesoporous silica channels, photophysics of new fluorescent rylene derivatives and synchronous emission on silver nanowires Eva Melari Davies |
| title_full_unstemmed | Single molecule microscopy investigations of highly oriented mesoporous silica channels, photophysics of new fluorescent rylene derivatives and synchronous emission on silver nanowires Eva Melari Davies |
| title_short | Single molecule microscopy |
| title_sort | single molecule microscopy investigations of highly oriented mesoporous silica channels photophysics of new fluorescent rylene derivatives and synchronous emission on silver nanowires |
| title_sub | investigations of highly oriented mesoporous silica channels, photophysics of new fluorescent rylene derivatives and synchronous emission on silver nanowires |
| topic_facet | Hochschulschrift |
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