Molecular biology: principles of genome function
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| Format: | Book |
|---|---|
| Language: | English |
| Published: |
Oxford
Oxford Univ. Press
2014
|
| Edition: | 2. ed. |
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | XXXI, 912 S. zahlr. Ill., graph. Darst. |
| ISBN: | 9780198705970 9780199658572 |
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Record in the Search Index
| _version_ | 1804152163423748096 |
|---|---|
| adam_text | Titel: Molecular biology
Autor: Craig, Nancy Lynn
Jahr: 2014
CONTENTS IN FULL
Abbreviations xxv
1 Genomes and the flow of biological information 1
Introduction 1
1.1 The roots of biology 1
1.2 The genome: a working blueprint for life 5
1.3 Bringing genes to life: gene expression 9
1.4 Regulating gene expression 12
1.5 Cellular infrastructure and gene expression 15
1.6 Expression of the genome 18
1.7 Evolution of the genome and the tree of life 21
2 Biological molecules 30
Introduction 30
2.1 Atoms, molecules, and chemical bonds 31
2.2 Life in aqueous solution 35
2.3 Non-covalent interactions 37
2.4 Nucleotides and nucleic acids 40
2.5 The structure of DNA 45
2.6 Chemical properties of RNA 53
2.7 RNA folding and structure 56
2.8 The RNA world and its role in the evolution of modern-day life 58
2.9 Fundamentals of protein structure 61
2.10 Protein folding 65
2.11 Protein folds 70
2.12 Protein-DNA interactions 72
2.13 Sugars and carbohydrates 74
2.14 Lipids 76
2.15 Chemical modification in biological regulation 80
3 The chemical basis of life 86
Introduction 86
3.1 Thermodynamic rules in biological systems 86
3.2 Binding equilibria and kinetics 94
3.3 Binding processes in biology 98
3.4 Enzyme catalysis 104
3.5 Enzyme kinetics 111
4 Chromosome structure and function 117
Introduction 117
4.1 Organization of chromosomes 118
4.2 The cell cycle and chromosome dynamics 120
4.3 Packaging chromosomal DNA 122
4.4 Variation in chromatin structure 129
4.5 Covalent modifications of histones 131
4.6 Nucleosome-remodeling complexes 137
4.7 DNA methylation 138
4.8 The separation of chromatin domains by boundary elements 142
4.9 Elements required for chromosome function 145
4.10 The centromere 146
4.11 The telomere 151
4.12 Chromosome architecture in the nucleus 154
5 The cell cycle 162
Introduction 162
5.1 Steps in the eukaryotic cell cycle 162
5.2 Cyclins and Cdks 166
5.3 Regulation of Cdk activity 171
5.4 Cell cycle regulation by Cdks 173
5.5 Regulation of proteolysis by Cdks 175
5.6 Checkpoints: intrinsic pathways that can halt the cell cycle 180
5.7 Extrinsic regulators of cell cycle progression 184
5.8 The cell cycle and cancer 186
5.9 The bacterial cell cycle 189
6 DNA replication 199
Introduction 199
6.1 Overview of DNA replication 199
6.2 DNA polymerases: structure and function 207
6.3 DNA polymerases: fidelity and processivity 210
6.4 Specialized polymerases 211
6.5 DNA helicases: unwinding of the double helix 213
6.6 The sliding clamp and clamp loader 218
6.7 Origins and initiation of DNA replication 221
6.8 Leading and lagging strand synthesis 224
6.9 The replication fork 227
6.10 Termination of DNA replication 231
6.11 The end-replication problem and telomerase 233
6.12 Chromatin replication 235
6.13 Regulation of initiation of replication in £ coli 238
6.14 Regulation of replication initiation in eukaryotes 241
7 Chromosome segregation 249
Introduction 249
7.1 The stages of mitosis 249
7.2 Chromosome condensation and cohesion 251
7.3 The mitotic spindle 257
7.4 Prometaphase and metaphase 263
7.5 Anaphase: an irreversible step in chromosome segregation 269
7.6 The completion of mitosis and cytokinesis 276
7.7 Meiosis: generating haploid gametes from diploid cells 278
7.8 Chromosome segregation in bacteria 285
8 Transcription 296
Introduction 296
8.1 Overview of transcription 296
8.2 RNA polymerase core enzyme 299
8.3 Promoter recognition in bacteria and eukaryotes 302
8.4 Initiation of transcription and transition to an elongating complex 313
8.5 Transcription elongation 319
8.6 Transcription termination 324
9 Regulation of transcription 330
Introduction 330
9.1 Principles of transcription regulation 330
9.2 DNA-binding domains in proteins that regulate transcription 335
9.3 Mechanisms for regulating transcription initiation in bacteria 340
9.4 Competition between cl and Cro and control of the fate of
bacteriophage lambda 345
9.5 Regulation of transcription termination in bacteria 351
9.6 Regulation of transcription initiation and elongation in eukaryotes 355
9.7 Combinatorial regulation of eukaryotic transcription 360
9.8 The role of signaling cascades in the regulation of transcription 363
9.9 Gene silencing 365
10 RNA processing 375
Introduction 375
10.1 Overview of RNA processing 375
10.2 tRNA and rRNA processing 378
10.3 tRNA and rRNA nucleotide modifications 383
10.4 mRNA capping and polyadenylation 385
10.5 RNA splicing 388
10.6 Eukaryotic mRNA splicing by the spliceosome 393
10.7 Exon definition and alternative splicing 399
10.8 RNA editing 405
10.9 Degradation of normal RNAs 410
10.10 Degradation of foreign and defective RNAs 413
10.11 RNA-binding domains in proteins 416
11 Translation 421
Introduction 421
11.1 Overview of translation 421
11.2 tRNA and the genetic code 423
11.3 Aminoacyl-tRNA synthetases 426
11.4 Structure of the ribosome 431
11.5 The translation cycle: the ribosome in action 435
11.6 Protein factors critical to the translation cycle 440
11.7 Translation initiation - shared features in bacteria and eukaryotes 443
11.8 Bacterial translation initiation 444
11.9 Eukaryotic translation initiation 448
11.10 Translation elongation: decoding, peptide bond formation, and translocation 450
11.11 Translation termination, recycling, and reinitiation 454
11.12 Ribosome rescue in bacteria and eukaryotes 460
11.13 Receding: programmed stop codon read-through and frameshifting 466
11.14 Antibiotics that target the ribosome 472
12 Regulation of translation 479
Introduction 479
12.1 Global regulation of initiation in bacteria and eukaryotes 479
12.2 Regulation of initiation by cis acting sequences in the 5 untranslated
region in bacteria and eukaryotes 482
12.3 Regulation of translation through cis acting sequences in the 3 UTR
in eukaryotes 489
12.4 Viral corruption of the translational machinery 492
13 Regulatory RNAs 498
Introduction 498
13.1 Overview of regulatory RNAs 501
13.2 Bacterial base-pairing sRNAs 503
13.3 Eukaryotic sRNAs: miRNAs, siRNAs, and rasiRNAs 506
13.4 Processing of eukaryotic sRNAs 507
13.5 Loading of Argonaute family proteins with eukaryotic sRNAs 516
13.6 Gene silencing by small eukaryotic RNAs 519
13.7 Viral defense role of bacterial, archaeal, and eukaryotic sRNAs 522
13.8 RNA-mediated regulation in cis 527
13.9 Protein-binding regulatory RNAs 530
13.10 Long intergenic non-coding RNAs 532
14 Protein modification and targeting 539
Introduction 539
14.1 Chaperone-assisted protein folding 539
14.2 Targeting of proteins throughout the cell 544
14.3 Post-translational cleavage of the polypeptide chain 548
14.4 Lipid modification of proteins 550
14.5 Glycosylation of proteins 557
14.6 Protein phosphorylation, acetylation, and methylation 560
14.7 Protein modification by nucleotides 567
14.8 Direct chemical modification of proteins 570
14.9 Ubiquitination and sumoylation of proteins 572
14.10 Protein degradation 579
15 Cellular responses to DNA damage 587
Introduction 587
15.1 Types of DNA damage 588
15.2 Post-replication mismatch repair 592
15.3 Repair of DNA damage by direct reversal 594
15.4 Repair of DNA damage by base excision repair 595
15.5 Nucleotide excision repair of bulky lesions 596
15.6 Translesion DNA synthesis 599
15.7 The DNA damage response 601
15.8 The DNA damage response in bacteria 605
15.9 The DNA damage response in eukaryotes 607
15.10 DNA damage and cell death in mammalian cells 618
16 Repair of DNA double-strand breaks and
homologous recombination 626
Introduction 626
16.1 An overview of DNA double-strand break repair and homologous
recombination 626
16.2 Double-strand break repair by NHEJ 628
16.3 Homology-directed repair of double-strand breaks 631
16.4 Generation of single-stranded DNA by helicases and nucleases 632
16.5 The mechanism of DNA strand pairing and exchange 637
16.6 Gene conversion through homology-directed repair 639
16.7 Repair of damaged replication forks by homology-directed repair 645
16.8 Homologous recombination 647
16.9 Chromosome rearrangements during aberrant repair and recombination 659
17 Mobile DNA 668
Introduction 668
17.1 Transposable elements: overview 668
17.2 An overview of DNA-only transposons 675
17.3 DNA-only cut-and-paste transposition 676
17.4 DNA-only nick-and-paste transposition 681
17.5 DNA cut-and-paste transposition in adaptive immunity 684
17.6 Retrotransposons 688
17.7 LTR retrotransposons 689
17.8 Non-LTR retrotransposons 693
17.9 Control of transposition 701
17.10 CSSR: overview 706
17.11 CSSR systems that control gene expression 710
17.12 CSSR conversion of DNA dimers to monomers 713
17.13 Bacteriophage lambda integration and excision 715
18 Genomics and genetic variation 726
Introduction 726
18.1 Genome sequences and sequencing projects 727
18.2 Finding functions in a genome 730
18.3 Functional genomics 734
18.4 The ENCODE project 739
18.5 The evolving genome: evolutionary forces 742
18.6 The evolving genome: mechanisms of variation 744
18.7 Gene duplication and divergence of gene function 749
18.8 Changes in chromosome structure and copy number variation 752
18.9 Epigenetics and imprinting 754
18.10 Human genetic diseases: finding disease loci 756
18.11 Human genetics: impacts and implications 764
19 Tools and techniques in molecular biology 775
Introduction 775
19.1 Model organisms 776
19.2 Cultured cells and viruses 784
19.3 Amplification of DNA and RNA sequences 788
19.4 DNA cloning 793
19.5 Genome manipulation 800
19.6 Detection of biological molecules 810
19.7 Separation and isolation of biological molecules 815
19.8 Identifying the composition of biological molecules 824
19.9 Detection of specific DNA sequences 831
19.10 Detection of specific RNA molecules 836
19.11 Detection of specific proteins 842
19.12 Detection of interactions between molecules 845
19.13 Imaging cells and molecules 853
19.14 Molecular structure determination 863
19.15 Obtaining and analyzing a complete genome sequence 866
Glossary 878
Index 901
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| spelling | Molecular biology principles of genome function Nancy L. Craig ... 2. ed. Oxford Oxford Univ. Press 2014 XXXI, 912 S. zahlr. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Genome Genomes Molecular Biology Molecular biology Molekularbiologie (DE-588)4039983-7 gnd rswk-swf Molekulargenetik (DE-588)4039987-4 gnd rswk-swf (DE-588)4123623-3 Lehrbuch gnd-content Molekulargenetik (DE-588)4039987-4 s DE-604 Molekularbiologie (DE-588)4039983-7 s Craig, Nancy Lynn 1952- Sonstige (DE-588)142435937 oth HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027273637&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
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| title | Molecular biology principles of genome function |
| title_auth | Molecular biology principles of genome function |
| title_exact_search | Molecular biology principles of genome function |
| title_full | Molecular biology principles of genome function Nancy L. Craig ... |
| title_fullStr | Molecular biology principles of genome function Nancy L. Craig ... |
| title_full_unstemmed | Molecular biology principles of genome function Nancy L. Craig ... |
| title_short | Molecular biology |
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| title_sub | principles of genome function |
| topic | Genome Genomes Molecular Biology Molecular biology Molekularbiologie (DE-588)4039983-7 gnd Molekulargenetik (DE-588)4039987-4 gnd |
| topic_facet | Genome Genomes Molecular Biology Molecular biology Molekularbiologie Molekulargenetik Lehrbuch |
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