Impact of virus-specific nonneutralizing antibodies on the clearance of Lymphocytic Choriomeningitis Virus:
Gespeichert in:
| 1. Verfasser: | |
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| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
2013 [erschienen] 2014
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| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | 85 Bl. Ill., graph. Darst. |
Internformat
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Datensatz im Suchindex
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| adam_text | SUMMARY 1
ABBREVIATIONS 2
1. INTRODUCTION 4
1.1 IMMUNITY AND THE LCMV MODEL SYSTEM 4
1.2 LCMV LIFE CYCLE 5
1.3 LCMV INFECTION AND THE ANTI-LCMV IMMUNE RESPONSE IN THE MOUSE 7
1.3.1 INNATE IMMUNITY TO LCMV 8
1.3.2 NATURAL ANTIBODIES AND LCMV 12
1.3.3 ADAPTIVE CELLULAR IMMUNITY TO LCMV 12
1.3.4 CD8
+
T-CELL EXHAUSTION 14
1.3.5 ADAPTIVE ANTIBODY-RESPONSE TO LCMV 17
1.3.6 NONNEUTRALIZING ANTIBODIES 19
1.3.7 NEUTRALIZING ANTIBODIES 21
1.4 THESIS OBJECTIVES 23
2. MATERIALS AND METHODS 24
2.1 MICE 24
2.2 CELL CULTURE 24
2.3 VIRUSES AND INFECTIONS 24
2.4 DETERMINATION OF VIRUS TITER 24
2.5 DETERMINATION OF VIRUS-NEUTRALIZING ACTIVITY 25
2.6 IN VIVO
IMMUNE SERUM TREATMENT 26
2.7 IN VIVO
ANTIBODY TREATMENT 26
2.8 FLOW CYTOMETRY 27
2.9 P14 TCR-TRANSGENIC CD8
+
T-CELL TRANSFER 28
2.10 ANTI-LCMV IGG ELISA 28
2.11 WESTERN BLOT 29
2.12
51
CROMIUM-RELEASE ASSAY 29
2.13 HISTOLOGY 30
2.14 STATISTICAL ANALYSIS 30
HTTP://D-NB.INFO/1049860497
3. RESULTS 31
3.1 NORMAL IMMUNE RESPONSE TO LCMV ARMSTRONG IN MICE WITH IMPAIRED
HUMORAL
IMMUNITY 31
3.2 DELAYED VIRAL CLEARANCE IN MD4 AND IGMI MICE AFTER LCMV WE INFECTION
32
3.3 CD8* T-CELL EXHAUSTION AND VIRUS PERSISTENCE AFTER LCMV DOCILE
INFECTION 34
3.4 VARIABLE EFFECT OF B-CELL DEPLETION ON LCMV CLEARANCE 34
3.5 IMMUNE SERUM TRANSFER IN MD4 AND IGMI MICE PARTIALLY RESCUES VIRUS
CLEARANCE 37
3.6 ANTIVIRAL ANTIBODIES ACCELERATED VIRUS ELIMINATION IN C57BL/6 MICE
38
3.7 NONNEUTRALIZING ANTIBODIES WITHIN THE IMMUNE SERUM ARE MAINLY
SPECIFIC FOR THE
LCMV NP 40
3.8 ANTI-LCMV NP ANTIBODIES EXHIBIT ANTIVIRAL ACTIVITY IN VIVO 42
3.9 NO VIRUS-REDUCING EFFECT OF ANTI-LCMV NP ANTIBODIES AFTER LCMV WE
INFECTION 44
3.10 LCMV NP EPITOPES ARE DETECTABLE ON THE SURFACE OF INFECTED CELLS 45
3.11 IDENTIFICATION OF LCMV NP IMMUNE-COMPLEXES ON THE SURFACE OF FEY
RECEPTOR* CELLS 48
3.12 ANTI LCMV NP ANTIBODY COLOCALIZES WITH INFECTED CELLS IN VIVO 49
3.13 LCMV IS-TRANSFER DOES NOT IMPROVE PRIMING OF LCMV-SPECIFIC CD8
+
T CELLS 51
3.14 THE ANTIVIRAL ACTIVITY OF NONNEUTRALIZING ANTI-LCMV ANTIBODIES IS
INDEPENDENT OF
FCYRS AND C3 COMPLEMENT 52
4. DISCUSSION 55
4.1 ANTIVIRAL ANTIBODIES ARE REQUIRED FOR THE ELIMINATION FAST
REPLICATING LCMV STRAINS 55
4.2 NONNEUTRALIZING ANTIBODIES ACCELERATE VIRUS CLEARANCE IN
IMMUNOCOMPETENT MICE 58
4.3 OUTLOOK 65
REFERENCES 67
PRESENTATIONS 84
PUBLICATION 84
ACKNOWLEDGEMENT I DANKSAGUNG 85
|
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| author | Straub, Tobias |
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| dewey-search | 610 570 |
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| dewey-tens | 610 - Medicine and health 570 - Biology |
| discipline | Biologie Medizin |
| format | Thesis Book |
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| spelling | Straub, Tobias Verfasser (DE-588)104794331X aut Impact of virus-specific nonneutralizing antibodies on the clearance of Lymphocytic Choriomeningitis Virus vorgelegt von Tobias Straub 2013 [erschienen] 2014 85 Bl. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Freiburg i. Br., Univ., Diss., 2014 Lymphozytäre Choriomeningitis (DE-588)4168377-8 gnd rswk-swf Antikörper (DE-588)4002290-0 gnd rswk-swf Humorale Immunität (DE-588)4160794-6 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Lymphozytäre Choriomeningitis (DE-588)4168377-8 s Humorale Immunität (DE-588)4160794-6 s Antikörper (DE-588)4002290-0 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027263546&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Straub, Tobias Impact of virus-specific nonneutralizing antibodies on the clearance of Lymphocytic Choriomeningitis Virus Lymphozytäre Choriomeningitis (DE-588)4168377-8 gnd Antikörper (DE-588)4002290-0 gnd Humorale Immunität (DE-588)4160794-6 gnd |
| subject_GND | (DE-588)4168377-8 (DE-588)4002290-0 (DE-588)4160794-6 (DE-588)4113937-9 |
| title | Impact of virus-specific nonneutralizing antibodies on the clearance of Lymphocytic Choriomeningitis Virus |
| title_auth | Impact of virus-specific nonneutralizing antibodies on the clearance of Lymphocytic Choriomeningitis Virus |
| title_exact_search | Impact of virus-specific nonneutralizing antibodies on the clearance of Lymphocytic Choriomeningitis Virus |
| title_full | Impact of virus-specific nonneutralizing antibodies on the clearance of Lymphocytic Choriomeningitis Virus vorgelegt von Tobias Straub |
| title_fullStr | Impact of virus-specific nonneutralizing antibodies on the clearance of Lymphocytic Choriomeningitis Virus vorgelegt von Tobias Straub |
| title_full_unstemmed | Impact of virus-specific nonneutralizing antibodies on the clearance of Lymphocytic Choriomeningitis Virus vorgelegt von Tobias Straub |
| title_short | Impact of virus-specific nonneutralizing antibodies on the clearance of Lymphocytic Choriomeningitis Virus |
| title_sort | impact of virus specific nonneutralizing antibodies on the clearance of lymphocytic choriomeningitis virus |
| topic | Lymphozytäre Choriomeningitis (DE-588)4168377-8 gnd Antikörper (DE-588)4002290-0 gnd Humorale Immunität (DE-588)4160794-6 gnd |
| topic_facet | Lymphozytäre Choriomeningitis Antikörper Humorale Immunität Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027263546&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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