Modern biophysical chemistry: detection and analysis of biomolecules
Saved in:
| Main Author: | |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
Weinheim
Wiley-VCH
2014
|
| Edition: | 2., updated and expanded ed. |
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | XIV, 344 S. Ill., graph. Darst. |
| ISBN: | 9783527337736 9783527683505 |
Staff View
MARC
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| 245 | 1 | 0 | |a Modern biophysical chemistry |b detection and analysis of biomolecules |c Peter Jomo Walla |
| 250 | |a 2., updated and expanded ed. | ||
| 264 | 1 | |a Weinheim |b Wiley-VCH |c 2014 | |
| 300 | |a XIV, 344 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
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Record in the Search Index
| _version_ | 1810074616260984832 |
|---|---|
| adam_text |
VII
CONTENTS
FOREWORD TO THE SECOND EDITION XIII
INTRODUCTION I
WHAT IS BIOPHYSICAL CHEMISTRY? - AN EXAMPLE FROM DRUG
SCREENING 1
PART ONE BASIC METHODS IN BIOPHYSICAL CHEMISTRY
11
1 BASIC OPTICAL PRINCIPLES 13
1.1 INTRODUCTION 13
1.2 WHAT DOES THE ELECTRONIC STRUCTURE OF MOLECULES LOOK LIKE?
ORBITALS, WAVE FUNCTIONS AND BONDING INTERACTIONS 15
1.3 HOW DOES LIGHT INTERACT WITH MOLECULES? TRANSITION DENSITIES
AND THE TRANSITION DIPOLE MOMENT 20
1.4 ABSORPTION SPECTRA OF MOLECULES IN LIQUID ENVIRONMENTS.
VIBRATIONAL EXCITATION AND THE FRANCK-CONDON PRINCIPLE 24
1.5 WHAT HAPPENS AFTER MOLECULES HAVE ABSORBED LIGHT? FLUORESCENCE,
NONRADIATIVE TRANSITIONS AND THE TRIPLET STATE 27
1.6 QUANTITATIVE DESCRIPTION OF ALL PROCESSES: QUANTUM EFFICIENCIES,
KINETICS OF EXCITED STATE POPULATIONS AND THE JABLONSKI DIAGRAM 33
PROBLEMS 38
BIBLIOGRAPHY 39
2 OPTICAL PROPERTIES OF BIOMOLECULES 41
2.1 INTRODUCTION 41
2.2 EXPERIMENTAL DETERMINATION OF ABSORPTION AND FLUORESCENCE
SPECTRA 41
2.3 OPTICAL PROPERTIES OF PROTEINS AND DNA 45
2.3.1 INTRINSIC ABSORPTION AND FLUORESCENCE OF AMINO ACIDS,
PEPTIDES AND PROTEINS 45
2.3.2 INTRINSIC ABSORPTION OF NUCLEOTIDES, DNA AND RNA 47
2.4 OPTICAL PROPERTIES OF IMPORTANT COFACTORS 49
HTTP://D-NB.INFO/1047989239
VIIII CONTENTS
2.4.1 HAEM 49
2.4.2 NICOTINAMIDE ADENINE DINUCLEOTIDES 52
2.4.3 FLAVINS 53
2.4.4 CHLOROPHYLLS 54
2.4.5 CAROTENOIDS 56
PROBLEMS 58
BIBLIOGRAPHY 58
3 BASIC FLUORESCENCE TECHNIQUES 61
3.1 INTRODUCTION 61
3.2 FLUORESCENT LABELLING AND LINKING TECHNIQUES 61
3.2.1 PRIMARY AMINO GROUP REACTIVE LABELS 63
3.2.2 THIOL GROUP REACTIVE LABELS 64
3.2.3 AVIDIN-BIOTIN TECHNIQUES 65
3.2.4 HIS-TAG
66
3.2.5 THIOLINKERS AND GOLD SURFACES 67
3.2.6 FLUORESCENT PROTEINS 67
3.3 FLUORESCENCE DETECTION TECHNIQUES
68
3.4 FLUORESCENCE POLARIZATION ANISOTROPY 70
3.4.1 PRINCIPLES AND THEORETICAL BACKGROUND 70
3.4.2 APPLICATION EXAMPLE: RECEPTOR-LIGAND INTERACTIONS 78
3.4.3 APPLICATION EXAMPLE: ESTIMATION OF MOLECULAR MASS 79
3.4.4 APPLICATION EXAMPLE: ENZYME FUNCTION AND KINETICS 80
3.4.5 APPLICATION EXAMPLE: ENZYME INHIBITION, ACTIVATION AND
REGULATION 83
3.5 FORSTER RESONANCE ENERGY TRANSFER 84
3.5.1 PRINCIPLES AND THEORETICAL BACKGROUND 84
3.5.2 APPLICATION EXAMPLES 90
3.6 FLUORESCENCE KINETICS 93
3.7 FLUORESCENCE RECOVERY AFTER PHOTOBLEACHING 98
3.8 BIOCHEMILUMINESCENCE 99
PROBLEMS 100
BIBLIOGRAPHY 103
4 CHIROPTICAL AND SCATTERING METHODS 105
- 4.1 CHIROPTICAL METHODS 105
4.1.1 CIRCULAR DICHROISM (CD) 105
4.1.2 OPTICAL ROTATORY DISPERSION 107
4.2 LIGHT SCATTERING 109
4.2.1 SCATTERING OF LIGHT AT MOLECULES SMALLER THAN THE OPTICAL
WAVELENGTH 110
4.2.2 SCATTERING OF LIGHT AT PARTICLES EQUAL TO OR LARGER THAN
THE OPTICAL WAVELENGTH 112
4.2.3 DYNAMIC LIGHT SCATTERING 115
4.3 VIBRATIONAL SPECTRA OF BIOMOLECULES 115
CONTENTS
| IX
PROBLEMS 118
BIBLIOGRAPHY 119
5 MAGNETIC RESONANCE TECHNIQUES 121
5.1 NUCLEAR MAGNETIC RESONANCE OF BIOMOLECULES 121
5.1.1 PRINCIPLES 121
5.1.2 THEORETICAL FRAMEWORK 123
5.1.3 PRIMARY INFORMATION DEDUCED FROM NMR SPECTRA 12S
5.1.4 PULSED NMR SPECTROSCOPY 126
5.1.5 TWO-DIMENSIONAL NMR SPECTROSCOPY 130
5.1.6 CORRELATED SPECTROSCOPY (COSY) 130
5.1.7 NUCLEAR OVERHAUSER EFFECT AND NOESY SPECTRA 135
5.1.8 NMR-BASED STRUCTURAL ANALYSIS OF BIOMOLECULES 138
5.2 ELECTRON PARAMAGNETIC RESONANCE 141
PROBLEMS 145
BIBLIOGRAPHY 147
6 MASS SPECTROMETRY 149
6.1 INTRODUCTION 149
6.2 MALDI-TOF 149
6.2.1 IONIZATION 149
6.2.2 ANALYSER 152
6.2.3 DETECTOR 153
6.2.4 SIGNALS AND SIGNAL IMPROVEMENTS 154
6.3 ESI-MS 156
6.3.1 IONIZATION 156
6.3.2 ANALYSER AND DETECTION 158
6.3.3 SIGNALS AND SIGNAL IMPROVEMENTS 161
6.4 STRUCTURAL AND SEQUENCE ANALYSIS USING MASS
SPECTROMETRY 163
PROBLEMS 164
BIBLIOGRAPHY 165
PART TWO ADVANCED METHODS IN BIOPHYSICAL CHEMISTRY
167
7 FLUORESCENCE MICROSCOPY 169
7.1 INTRODUCTION 169
7.2 CONVENTIONAL FLUORESCENCE MICROSCOPY 169
7.2.1 CONFOCAL FLUORESCENCE MICROSCOPY 169
7.2.2 LASER SCANNING MICROSCOPY 174
7.2.3 WIDE-FIELD FLUORESCENCE MICROSCOPY 174
7.3 TOTAL INTERNAL REFLECTION FLUORESCENCE MICROSCOPY 176
7.4 LIGHT-SHEET MICROSCOPY 178
PROBLEMS 180
BIBLIOGRAPHY 181
X| CONTENTS
8 SUPER-RESOLUTION FLUORESCENCE MICROSCOPY 183
8.1 STIMULATED EMISSION DEPLETION (STED) MICROSCOPY 184
8.2 PHOTOACTIVATED LOCALIZATION MICROSCOPY (PALM) AND
STOCHASTIC OPTICAL RECONSTRUCTION MICROSCOPY (STORM) 187
8.3 3D SUPER-RESOLUTION FLUORESCENCE MICROSCOPY 190
8.3.1 3D-STED 190
8.3.2 3D-PALM/STORM 191
8.4 IMAGING OF LIVE CELLS 191
8.4.1 OBSERVATION DURATION 192
8.4.2 IRRADIATION INTENSITY 193
8.4.3 IMAGING DEPTHS 194
8.4.4 LABELLING CONDITIONS 194
8.5 MULTICOLOUR SUPER-RESOLUTION FLUORESCENCE MICROSCOPY 195
8.6 STRUCTURED ILLUMINATION MICROSCOPY 195
8.7 SOFI 197
8.8 FINAL COMPARISON 199
PROBLEMS 201
BIBLIOGRAPHY 202
9 SINGLE-BIOMOLECULE TECHNIQUES 203
9.1 INTRODUCTION 203
9.2 OPTICAL SINGLE-MOLECULE DETECTION 203
9.2.1 APPLICATION EXAMPLE 1: OBSERVATION OF THE ROTATION OF SINGLE
ATPASE COMPLEXES 206
9.2.2 APPLICATION EXAMPLE 2: SINGLE-MOLECULE OBSERVATION
OF THE ELEMENTARY STEPS OF BIOMOLECULAR MOTORS 209
9.3 FLUORESCENCE CORRELATION SPECTROSCOPY 213
9.3.1 AUTOCORRELATION ANALYSIS AND OBSERVABLE KEY PARAMETERS 214
9.3.2 AUTOCORRELATION ANALYSIS, MATHEMATICAL BACKGROUND 220
9.3.3 QUANTITATIVE DETERMINATION OF IMPORTANT PARAMETERS
FROM AUTOCORRELATION CURVES 221
9.3.3.1 RELATIONSHIP BETWEEN VOLUME AND MOLECULAR MASS OF A
PROTEIN AND ITS DIFFUSION TIME 222
9.3.3.2 TWO-DIMENSIONAL DIFFUSION AND ACTIVE TRANSPORT 222
9.3.3.3 MIXTURES OF FLUORESCING PARTICLES 223
9.3.4 FURTHER CORRELATION EFFECTS 224
9.3.5 CROSS-CORRELATION ANALYSIS 226
9.4 OPTICAL TWEEZERS 230
9.4.1 THEORETICAL BACKGROUND 230
9.4.2 APPLICATION EXAMPLES 236
9.4.2.1 UNFOLDING OF RNA AND DNA HAIRPINS 236
9.4.2.2 RNA POLYMERASE 238
9.4.2.3 DNA-POLYMERASE 239
9.5 ATOMIC FORCE MICROSCOPY OF BIOMOLECULES 240
9.5.1 PRINCIPLE OF AN AFM 241
CONTENTS
9.5.2 APPLICATION EXAMPLES 242
9.5.2.1 UNFOLDING OF DNA HAIRPINS 242
9.5.2.2 RECEPTOR-LIGAND BINDING FORCES 244
9.5.2.3 PROTEIN UNFOLDING 244
9.6 PATCH CLAMPING 245
9.6.1 ION CHANNELS 245
9.6.2 PATCH CLAMP CONFIGURATIONS 246
PROBLEMS 250
BIBLIOGRAPHY 25 4
10 ULTRAFAST- AND NONLINEAR SPECTROSCOPY 257
10.1 INTRODUCTION 257
10.2 NONLINEAR MICROSCOPY AND SPECTROSCOPY 258
10.2.1 MULTIPHOTON EXCITATION 258
10.2.2 ADVANTAGES AND DISADVANTAGES OF TWO-PHOTON EXCITATION
IN FLUORESCENCE MICROSCOPY 259
10.2.3 HOW ARE NONLINEAR OPTICAL SIGNALS OBSERVED FROM
BIOLOGICAL SAMPLES? 262
10.2.4 FURTHER DISTINCT PROPERTIES AND ADVANTAGES OF TWO-PHOTON
EXCITATION 263
10.2.5 WAVEMIXING AND OTHER NONLINEAR OPTICAL TECHNIQUES 266
10.3 ULTRAFAST SPECTROSCOPY : 270
10.3.1 PUMP-PROBE SPECTROSCOPY 270
10.3.2 APPLICATION EXAMPLE: ULTRAFAST LIGHT-HARVESTING AND ENERGY
CONVERSION IN PHOTOSYNTHESIS 274
10.3.2.1 CHLOROPHYLL B *CHLOROPHYLL A * REACTION CENTRE ENERGY FLOW
276
10.3.2.2 CAROTENOID ** CHLOROPHYLL ENERGY FLOW 278
PROBLEMS 280
BIBLIOGRAPHY 282
11 DNA SEQUENCING AND NEXT-GENERATION SEQUENCING METHODS 285
11.1 SANGER METHOD 285
11.2 NEXT-GENERATION SEQUENCING METHODS 287
11.2.1 DYE SEQUENCING (APPROACH I) 289
11.2.2 SEQUENCING BY LIGATION, PYROSEQUENCING AND ION SEMICONDUCTOR
SEQUENCING (APPROACHES II TO IV) 292
11.2.2.1 EMULSION PCR 292
11.2.2.2 SEQUENCING BY LIGATION (APPROACH II) 294
11.2.2.3 PYROSEQUENCING (APPROACH III) 296
11.2.2.4 ION SEMICONDUCTOR SEQUENCING (APPROACH IV) 297
11.2.3 SINGLE-MOLECULE REAL-TIME SEQUENCING (APPROACH V) 299
PROBLEMS 300
BIBLIOGRAPHY 301
12
12.1
SPECIAL TECHNIQUES 303
INTRODUCTION 303
XII
| CONTENTS
12.2 FLUORESCING NANOPARTICLES 303
12.3 SURFACE PLASMON RESONANCE DETECTION 308
12.4 DNA ORIGAMI 310
12.5 DNA MICROARRAYS 314
12.6 FLOW CYTOMETRY 317
12.7 FLUORESCENCE IN SITU HYBRIDIZATION 319
12.8 MICROSPHERES AND NANOSPHERES 320
PROBLEMS 321
BIBLIOGRAPHY 321
13 ASSAY DEVELOPMENT, READERS AND HIGH-THROUGHPUT SCREENING 323
13.1 INTRODUCTION 323
13.2 ASSAY DEVELOPMENT AND ASSAY QUALITY 323
13.3 MICROTITRE PLATES AND FLUORESCENCE READERS 326
13.4 APPLICATION EXAMPLE: DRUG DISCOVERY AND HIGH-THROUGHPUT
SCREENING 332
PROBLEMS 336
BIBLIOGRAPHY 337
INDEX.
339 |
| any_adam_object | 1 |
| author | Walla, Peter Jomo 1970- |
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| dewey-ones | 571 - Physiology & related subjects |
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| discipline | Biologie |
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| id | DE-604.BV041817235 |
| illustrated | Illustrated |
| indexdate | 2024-09-13T10:01:12Z |
| institution | BVB |
| isbn | 9783527337736 9783527683505 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-027262435 |
| oclc_num | 890015438 |
| open_access_boolean | |
| owner | DE-11 DE-188 DE-703 DE-20 DE-92 DE-355 DE-BY-UBR DE-19 DE-BY-UBM |
| owner_facet | DE-11 DE-188 DE-703 DE-20 DE-92 DE-355 DE-BY-UBR DE-19 DE-BY-UBM |
| physical | XIV, 344 S. Ill., graph. Darst. |
| publishDate | 2014 |
| publishDateSearch | 2014 |
| publishDateSort | 2014 |
| publisher | Wiley-VCH |
| record_format | marc |
| spelling | Walla, Peter Jomo 1970- Verfasser (DE-588)120817691 aut Modern biophysical chemistry detection and analysis of biomolecules Peter Jomo Walla 2., updated and expanded ed. Weinheim Wiley-VCH 2014 XIV, 344 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Biophysikalische Chemie (DE-588)4291844-3 gnd rswk-swf Biophysikalische Chemie (DE-588)4291844-3 s DE-604 Erscheint auch als Online-Ausgabe, EPUB 978-3-527-68355-0 Erscheint auch als Online-Ausgabe, MOBI 978-3-527-68356-7 Erscheint auch als Online-Ausgabe, PDF 978-3-527-68354-3 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027262435&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Walla, Peter Jomo 1970- Modern biophysical chemistry detection and analysis of biomolecules Biophysikalische Chemie (DE-588)4291844-3 gnd |
| subject_GND | (DE-588)4291844-3 |
| title | Modern biophysical chemistry detection and analysis of biomolecules |
| title_auth | Modern biophysical chemistry detection and analysis of biomolecules |
| title_exact_search | Modern biophysical chemistry detection and analysis of biomolecules |
| title_full | Modern biophysical chemistry detection and analysis of biomolecules Peter Jomo Walla |
| title_fullStr | Modern biophysical chemistry detection and analysis of biomolecules Peter Jomo Walla |
| title_full_unstemmed | Modern biophysical chemistry detection and analysis of biomolecules Peter Jomo Walla |
| title_short | Modern biophysical chemistry |
| title_sort | modern biophysical chemistry detection and analysis of biomolecules |
| title_sub | detection and analysis of biomolecules |
| topic | Biophysikalische Chemie (DE-588)4291844-3 gnd |
| topic_facet | Biophysikalische Chemie |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027262435&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT wallapeterjomo modernbiophysicalchemistrydetectionandanalysisofbiomolecules |