Staphylococcus epidermidis: methods and protocols
Due to the highly collaborative nature of investigators working in the field, we have rapidly advanced our understanding of Staphylococcus epidermidis and other staphylococci in the last two decades. The chapters in Staphylococcus Epidermidis: Methods and Protocols are designed to give the new inves...
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
New York [u.a.]
Springer
2014
|
| Schriftenreihe: | Methods in molecular biology
1106 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Zusammenfassung: | Due to the highly collaborative nature of investigators working in the field, we have rapidly advanced our understanding of Staphylococcus epidermidis and other staphylococci in the last two decades. The chapters in Staphylococcus Epidermidis: Methods and Protocols are designed to give the new investigator a series of tools so they can ask novel and exciting questions related to the biology of this opportunistic pathogen, as many exciting and unexplored questions such as defining the interaction of S. epidermidis and other normal flora remain to be discovered. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls |
| Beschreibung: | Includes bibliographical references and index Clinical characteristics of infections in humans due to Staphylococcus epidermidis / Mark E. Rupp -- Staphylococcus epidermidis pathogenesis / Michael Otto -- Identification of Staphylococcus epidermidis in the clinical microbiology laboratory by molecular methods / Amity L. Roberts -- Pulsed field gel electrophoresis of Staphylococcus epidermidis / Richard V. Goering and Paul D. Fey -- Multilocus sequence typing of Staphylococcus epidermidis / Jonathan C. Thomas and D. Ashley Robinson -- Growth and preparation of Staphylococcus epidermidis for NMR metabolomic analysis / Greg A. Somerville and Robert Powers -- The isolation and analysis of phenol-soluble modulins of Staphylococcus epidermidis / Hwang-Soo Joo and Michael Otto -- Genetic manipulation of staphylococci / Jeffrey L. Bose -- Isolation of chromosomal and plasmid DNA from Staphylococcus epidermidis / Jill K. Lindgren -- Isolation of Staphylococus sp. RNA / Tess Eidem -- Use of electroporation and conjugative mobilization for genetic manipulation of Staphylococcus epidermidis / Katherine L. Maliszewski and Austin S. Nuxoll -- Methods to generate a sequence-defined transposon mutant library in Staphylococcus epidermidis strain 1457 / Todd J. Widhelm [and four others] -- Examination of Staphylococcus epidermidis biofilms using flow-cell technology / Derek E. Moormeier and Kenneth W. Bayles -- Rapid quantitative and qualitative analysis of biofilm production by Staphylococcus epidermidis under static growth conditions / Elaine M. Waters [and five others] -- Bacteriophage transduction in Staphylococcus epidermidis / Michael E. Olson and Alexander R. Horswill -- Mouse model of post-arthroplasty Staphylococcus epidermidis joint infection / Tyler D. Scherr [and five others] -- A mouse model of Staphylococcus catheter-associated biofilm infection / Cortney E. Heim, Mark L. Hanke, and Tammy Kielian -- Generation of a central nervous system catheter-associated infection in mice with Staphylococcus epidermidis / Jessica N. Snowden -- Rat jugular catheter model of biofilm-mediated infection / Carolyn R. Schaeffer, Keith M. Woods, and G. Matthew Longo |
| Beschreibung: | X, 208 p. Ill., graph. Darst. 26 cm |
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| 500 | |a Clinical characteristics of infections in humans due to Staphylococcus epidermidis / Mark E. Rupp -- Staphylococcus epidermidis pathogenesis / Michael Otto -- Identification of Staphylococcus epidermidis in the clinical microbiology laboratory by molecular methods / Amity L. Roberts -- Pulsed field gel electrophoresis of Staphylococcus epidermidis / Richard V. Goering and Paul D. Fey -- Multilocus sequence typing of Staphylococcus epidermidis / Jonathan C. Thomas and D. Ashley Robinson -- Growth and preparation of Staphylococcus epidermidis for NMR metabolomic analysis / Greg A. Somerville and Robert Powers -- The isolation and analysis of phenol-soluble modulins of Staphylococcus epidermidis / Hwang-Soo Joo and Michael Otto -- Genetic manipulation of staphylococci / Jeffrey L. Bose -- Isolation of chromosomal and plasmid DNA from Staphylococcus epidermidis / Jill K. Lindgren -- Isolation of Staphylococus sp. RNA / Tess Eidem -- | ||
| 500 | |a Use of electroporation and conjugative mobilization for genetic manipulation of Staphylococcus epidermidis / Katherine L. Maliszewski and Austin S. Nuxoll -- Methods to generate a sequence-defined transposon mutant library in Staphylococcus epidermidis strain 1457 / Todd J. Widhelm [and four others] -- Examination of Staphylococcus epidermidis biofilms using flow-cell technology / Derek E. Moormeier and Kenneth W. Bayles -- Rapid quantitative and qualitative analysis of biofilm production by Staphylococcus epidermidis under static growth conditions / Elaine M. Waters [and five others] -- Bacteriophage transduction in Staphylococcus epidermidis / Michael E. Olson and Alexander R. Horswill -- Mouse model of post-arthroplasty Staphylococcus epidermidis joint infection / Tyler D. Scherr [and five others] -- A mouse model of Staphylococcus catheter-associated biofilm infection / Cortney E. Heim, Mark L. Hanke, and Tammy Kielian -- | ||
| 500 | |a Generation of a central nervous system catheter-associated infection in mice with Staphylococcus epidermidis / Jessica N. Snowden -- Rat jugular catheter model of biofilm-mediated infection / Carolyn R. Schaeffer, Keith M. Woods, and G. Matthew Longo | ||
| 520 | |a Due to the highly collaborative nature of investigators working in the field, we have rapidly advanced our understanding of Staphylococcus epidermidis and other staphylococci in the last two decades. The chapters in Staphylococcus Epidermidis: Methods and Protocols are designed to give the new investigator a series of tools so they can ask novel and exciting questions related to the biology of this opportunistic pathogen, as many exciting and unexplored questions such as defining the interaction of S. epidermidis and other normal flora remain to be discovered. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls | ||
| 650 | 4 | |a Staphylococcus epidermidis / Laboratory Manuals | |
| 650 | 4 | |a Staphylococcal Infections / Laboratory Manuals | |
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Datensatz im Suchindex
| _version_ | 1804152040014741504 |
|---|---|
| adam_text | Titel: Staphylococcus epidermidis
Autor: Fey, Paul D
Jahr: 2014
Contents
Preface.............................................................. v
Contributors.......................................................... ix
1 Clinical Characteristics of Infections in Humans
Due to Staphylococcus epidermidis......................................... 1
Mark E. Rupp
2 Staphylococcus epidermidis Pathogenesis.................................. 17
Michael Otto
3 Identification of Staphylococcus epidermidis in the Clinical Microbiology
Laboratory by Molecular Methods..................................... 33
Amity L. Roberts
4 Pulsed Field Gel Electrophoresis of Staphylococcus epidermidis.................. 55
Richard V. Goering and Paul D. Fey
5 Multilocus Sequence Typing of Staphylococcus epidermidis..................... 61
Jonathan C. Thomas and D. Ashley Robinson
6 Growth and Preparation of Staphylococcus epidermidis
for NMR Metabolomic Analysis....................................... 71
Greg A. Somerville and Robert Powers
7 The Isolation and Analysis of Phenol-Soluble Modulins
of Staphylococcus epidermidis............................................. 93
Hwang-Soo Joo and Michael Otto
8 Genetic Manipulation of Staphylococci.................................. 101
Jeffrey L. Bose
9 Isolation of Chromosomal and Plasmid DNA from Staphylococcus epidermidis..... 113
Jill K. Lindgren
10 Isolation of Staphylococcus sp. RNA..................................... 119
Tess Eidem
11 Use of Electroporation and Conjugative Mobilization
for Genetic Manipulation of Staphylococcus epidermidis........................ 125
Katherine L. Maliszewski and Austin S. Nuxoll
12 Methods to Generate a Sequence-Defined Transposon Mutant
Library in Staphylococcus epidermidis Strain 1457........................... 135
Todd J. Widhelm, Vijay Kumar Yajjala, Jennifer L. Endres,
Paul D. Fey, and Kenneth W. Bayles
13 Examination of Staphylococcus epidermidis Biofilms
Using Flow-Cell Technology......................................... 143
Derek E. Moormeier and Kenneth W Bayles
vii
viii
Contents
14 Rapid Quantitative and Qualitative Analysis of Biofilm Production
by Staphylococcus epidermidis Under Static Growth Conditions................ 157
Elaine M. Waters, Hannah McCarthy, Siobhan Hogan,
Marta Zapotoczna, Eoghan O Neill, and James P. O Gara
15 Bacteriophage Transduction in Staphylococcus epidermidis...................... 167
Michael E. Olson and Alexander R. Horswill
16 Mouse Model of Post-arthroplasty Staphylococcus epidermidis Joint Infection...... 173
Tyler D. Scherr, Kevin E. Lindjjren, Carolyn R. Schaejfer,
Mark L. Hanke, Curtis W. Hartman, and Tammy Kielian
17 A Mouse Model of Staphylococcus Catheter-Associated Biofilm Infection......... 183
Cortney E. Heim, Mark L. Hanke, and Tammy Kielian
18 Generation of a Central Nervous System Catheter-Associated Infection in Mice
with Staphylococcus epidermidis........................................... 193
Jessica N. Snowden
19 Rat Jugular Catheter Model of Biofilm-Mediated Infection................... 199
Carolyn R. Schaejfer, Keith M. Woods, and G. Matthew Longo
Index................................................................... 207
|
| any_adam_object | 1 |
| author | Fey, Paul D. |
| author_GND | (DE-588)1049616901 |
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| dewey-full | 616.92 |
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| dewey-ones | 616 - Diseases |
| dewey-raw | 616.92 |
| dewey-search | 616.92 |
| dewey-sort | 3616.92 |
| dewey-tens | 610 - Medicine and health |
| discipline | Medizin |
| format | Book |
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| indexdate | 2024-07-10T01:04:23Z |
| institution | BVB |
| language | English |
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| physical | X, 208 p. Ill., graph. Darst. 26 cm |
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| series2 | Methods in molecular biology Springer protocols |
| spelling | Fey, Paul D. Verfasser (DE-588)1049616901 aut Staphylococcus epidermidis methods and protocols ed. by Paul D. Fey New York [u.a.] Springer 2014 X, 208 p. Ill., graph. Darst. 26 cm txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 1106 Springer protocols Includes bibliographical references and index Clinical characteristics of infections in humans due to Staphylococcus epidermidis / Mark E. Rupp -- Staphylococcus epidermidis pathogenesis / Michael Otto -- Identification of Staphylococcus epidermidis in the clinical microbiology laboratory by molecular methods / Amity L. Roberts -- Pulsed field gel electrophoresis of Staphylococcus epidermidis / Richard V. Goering and Paul D. Fey -- Multilocus sequence typing of Staphylococcus epidermidis / Jonathan C. Thomas and D. Ashley Robinson -- Growth and preparation of Staphylococcus epidermidis for NMR metabolomic analysis / Greg A. Somerville and Robert Powers -- The isolation and analysis of phenol-soluble modulins of Staphylococcus epidermidis / Hwang-Soo Joo and Michael Otto -- Genetic manipulation of staphylococci / Jeffrey L. Bose -- Isolation of chromosomal and plasmid DNA from Staphylococcus epidermidis / Jill K. Lindgren -- Isolation of Staphylococus sp. RNA / Tess Eidem -- Use of electroporation and conjugative mobilization for genetic manipulation of Staphylococcus epidermidis / Katherine L. Maliszewski and Austin S. Nuxoll -- Methods to generate a sequence-defined transposon mutant library in Staphylococcus epidermidis strain 1457 / Todd J. Widhelm [and four others] -- Examination of Staphylococcus epidermidis biofilms using flow-cell technology / Derek E. Moormeier and Kenneth W. Bayles -- Rapid quantitative and qualitative analysis of biofilm production by Staphylococcus epidermidis under static growth conditions / Elaine M. Waters [and five others] -- Bacteriophage transduction in Staphylococcus epidermidis / Michael E. Olson and Alexander R. Horswill -- Mouse model of post-arthroplasty Staphylococcus epidermidis joint infection / Tyler D. Scherr [and five others] -- A mouse model of Staphylococcus catheter-associated biofilm infection / Cortney E. Heim, Mark L. Hanke, and Tammy Kielian -- Generation of a central nervous system catheter-associated infection in mice with Staphylococcus epidermidis / Jessica N. Snowden -- Rat jugular catheter model of biofilm-mediated infection / Carolyn R. Schaeffer, Keith M. Woods, and G. Matthew Longo Due to the highly collaborative nature of investigators working in the field, we have rapidly advanced our understanding of Staphylococcus epidermidis and other staphylococci in the last two decades. The chapters in Staphylococcus Epidermidis: Methods and Protocols are designed to give the new investigator a series of tools so they can ask novel and exciting questions related to the biology of this opportunistic pathogen, as many exciting and unexplored questions such as defining the interaction of S. epidermidis and other normal flora remain to be discovered. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls Staphylococcus epidermidis / Laboratory Manuals Staphylococcal Infections / Laboratory Manuals Staphylococcal infections Methods in molecular biology 1106 (DE-604)BV035362695 1106 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027191941&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Fey, Paul D. Staphylococcus epidermidis methods and protocols Methods in molecular biology Staphylococcus epidermidis / Laboratory Manuals Staphylococcal Infections / Laboratory Manuals Staphylococcal infections |
| title | Staphylococcus epidermidis methods and protocols |
| title_auth | Staphylococcus epidermidis methods and protocols |
| title_exact_search | Staphylococcus epidermidis methods and protocols |
| title_full | Staphylococcus epidermidis methods and protocols ed. by Paul D. Fey |
| title_fullStr | Staphylococcus epidermidis methods and protocols ed. by Paul D. Fey |
| title_full_unstemmed | Staphylococcus epidermidis methods and protocols ed. by Paul D. Fey |
| title_short | Staphylococcus epidermidis |
| title_sort | staphylococcus epidermidis methods and protocols |
| title_sub | methods and protocols |
| topic | Staphylococcus epidermidis / Laboratory Manuals Staphylococcal Infections / Laboratory Manuals Staphylococcal infections |
| topic_facet | Staphylococcus epidermidis / Laboratory Manuals Staphylococcal Infections / Laboratory Manuals Staphylococcal infections |
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