Functional analyses of ES cell pluripotency by inducible knockdown of the Polycomb group protein Pcgf6:
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| Main Author: | |
|---|---|
| Format: | Thesis Book |
| Language: | English |
| Published: |
2013
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| Subjects: | |
| Online Access: | Volltext Inhaltsverzeichnis |
| Item Description: | Zsfassung in dt. und engl. Sprache |
| Physical Description: | 77 Bl. Ill., graph. Darst. |
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MARC
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| 100 | 1 | |a Li, Xiaoli |d 1980- |e Verfasser |0 (DE-588)104730547X |4 aut | |
| 245 | 1 | 0 | |a Functional analyses of ES cell pluripotency by inducible knockdown of the Polycomb group protein Pcgf6 |c von Xiaoli Li |
| 246 | 1 | 3 | |a Functionelle Analysen der ES-Zell-Pluripotenz durch induzierbaren Knockdown des Polycomb group Proteins Pcgf6 |
| 264 | 1 | |c 2013 | |
| 300 | |a 77 Bl. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
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| 500 | |a Zsfassung in dt. und engl. Sprache | ||
| 502 | |a Würzburg, Univ., Diss., 2013 | ||
| 650 | 0 | 7 | |a Embryonale Stammzelle |0 (DE-588)4699648-5 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Pluripotenz |0 (DE-588)4420540-5 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Epigenetik |0 (DE-588)7566079-9 |2 gnd |9 rswk-swf |
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| 689 | 0 | 0 | |a Embryonale Stammzelle |0 (DE-588)4699648-5 |D s |
| 689 | 0 | 1 | |a Pluripotenz |0 (DE-588)4420540-5 |D s |
| 689 | 0 | 2 | |a Epigenetik |0 (DE-588)7566079-9 |D s |
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Record in the Search Index
| _version_ | 1804151860428275712 |
|---|---|
| adam_text | TABLE OF CONTENT
1 SUMMARY 5
2 ZUSAMMENFASSUNG 6
3 ABBREVIATIONS 7
4 INTRODUCTION 9
4.1 STEM CELLS 9
4.1.1 THE STEM CELL SYSTEM IN VIVO 9
4.1.2 EMBRYONIC STEM CELLS 10
4.1.3 THE CORE PLURIPOTENCY NETWORK 11
4.1.4 CHROMATIN STRUCTURE IN ES CELLS 12
4.2 POLYCOMB FACTORS 14
4.2.1 POLYCOMB GROUP REPRESSIVE COMPLEXES 14
4.2.2 HETEROGENEITY OF MAMMALIAN PRC1 16
4.2.3 PRC1 IN ES CELLS 17
4.2.4 PARALOGS OF POLYCOMB GROUP RING FINGER PROTEINS 19
4.2.5 PRC1 COMPONENTS IN HEMATOPOIESIS 19
4.3 SCIENTIFIC AIM AND STRATEGY 20
5 RESULTS 21
5.1 THE PCGF PARALOG PCGF6 IS HIGHLY EXPRESSED IN ES CELLS 21
5.2 GENERATION OF INDUCIBLE PCGF6 SHRNA ES CELLS 22
5.3 KNOCKDOWN EFFICIENCY OF PCGF6-SHRNAS 25
5.4 PCGF6 KNOCKDOWN REDUCES ES CELL COLONY FORMING ABILITY 26
5.5 APOPTOSIS RATE, CELL CYCLE DISTRIBUTION AND PROLIFERATION WERE NOT
ALTERED UPON
PCGF6 KNOCKDOWN 28
5.6 GLOBAL HISTONE MODIFICATIONS IN ES CELLS UPON PCGF6 KNOCKDOWN 29
5.7 PCGF6 KNOCKDOWN INCREASES EXPRESSION OF DEVELOPMENTAL AND TESTIS
SPECIFIC
GENES IN ES CELLS 30
5.8 PCGF6 KNOCKDOWN IN ES CELLS CAUSED TRANSIENTLY INCREASED
UP-REGULATION OF
EARLY MESODERMAL/HEMATOPOIETIC MARKER GENES DURING ES CELL
DIFFERENTIATION 35
5.9 PCGF6 KNOCKDOWN INCREASES ES CELL HEMANGIOBLAST DIFFERENTIATION 36
5.10 PCGF6 KNOCKDOWN ENHANCES ES CELL DIFFERENTIATION TOWARDS
HEMATOPOIETIC
LINEAGES 38
6 DISCUSSION 39
6.1 DIFFERENTIAL GENE EXPRESSION OF PCGF PARALOGS SUGGESTS
PARALOG-SPECIFIC
FUNCTIONS 39
6.2 SHRNA-BASED PCGF6 KNOCKDOWN IN ES CELLS WAS INDUCIBLE AND REVERSIBLE
40
6.3 PCGF6 KNOCKDOWN LED TO REDUCED ES CELL COLONY FORMATION, BUT DID NOT
AFFECT ES
CELL MORPHOLOGY, PROLIFERATION, CELL CYCLE DISTRIBUTION OR CELL SURVIVAL
40
6.4 PCGF6 IS NOT REQUIRED FOR GENE EXPRESSION OF OTHER PCGF PARALOGS,
PRC1 OR PRC2
CORE SUBUNITS IN ES CELLS 41
6.5 PCGF6 IS INVOLVED IN REGULATING EXPRESSION OF PLURIPOTENCY GENES
0CT4, SOX2 AND
NANOG 41
6.6 PCGF6 IS REQUIRED FOR THE REPRESSION OF DIFFERENTIATION-ASSOCIATED
GENES AND
SPERMATOGENESIS-SPECIFIC GENES 42
6.7 PCGF6 IS NOT REQUIRED FOR MAINTAINING THE GLOBAL LEVEL OF H2AK119
HISTONE
MARKS 44
3
HTTP://D-NB.INFO/1048444295
6.8 PCGF6 IS INVOLVED IN MAINTAINING ES CELL IDENTITY BY PREVENTING
MESODERMAL/
HEMATOPOIETIC DIFFERENTIATION 44
6.9 FUTURE PERSPECTIVES 45
6.9.1 OVEREXPRESSION OF PCGF6 IN ES CELLS WILL PROVIDE MORE INSIGHT INTO
THE ROLES OF
PCGF6 IN MAINTAINING ES CELL IDENTITY 45
6.9.2 PROTEOMIC ANALYSIS WILL REVEAL INTERACTION PARTNERS OF PCGF6 46
6.9.3 THE ROLE OF PCGF6 IN EMBRYONIC SPERMATOGENESIS WILL BE ADDRESSED
VIA ES
CELL-DERIVED PRIMORDIAL GERM CELL LIKE CELL (PGCLC) DIFFERENTIATION 46
7 MATERIAL AND METHODS 47
7.1 MATERIAL 47
7.1.1 MOUSE ES CELL LINES 47
7.1.2 CELL CULTURE MEDIA AND SUPPLEMENTS 47
7.1.3 ANTIBODIES 49
7.1.4 ENZYMES 49
7.1.5 PRIMERS 50
7.1.6 PLASMID VECTORS 51
7.1.7 SHRNA SEQUENCES 51
7.1.8 BUFFERS AND SOLUTIONS 52
7.1.9 COMMERCAIL KITS AND REAGENTS : 53
7.1.10 PLASTIC CONSUMABLES, DEVICES AND SOFTWARE 53
7.2 METHODS 54
7.2.1 CELL CULTURE AND DIFFERENTIATION 54
7.2.2 FACS STAINING 57
7.2.3 ISOLATION OF MOUSE TISSUES 58
7.2.4 MOLECULAR BIOLOGY 58
8 REFERENCES 62
9 ACKNOWLEDGEMENTS 71
10 AFFIDAVIT 72
11 CURRICULUM VITAE 74
12 PUBLICATIONS 75
13 ATTACHMENT 76
PLASMID MAPS 76
4
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| ctrlnum | (OCoLC)873463765 (DE-599)BVBBV041638939 |
| discipline | Biologie |
| format | Thesis Book |
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| spelling | Li, Xiaoli 1980- Verfasser (DE-588)104730547X aut Functional analyses of ES cell pluripotency by inducible knockdown of the Polycomb group protein Pcgf6 von Xiaoli Li Functionelle Analysen der ES-Zell-Pluripotenz durch induzierbaren Knockdown des Polycomb group Proteins Pcgf6 2013 77 Bl. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Zsfassung in dt. und engl. Sprache Würzburg, Univ., Diss., 2013 Embryonale Stammzelle (DE-588)4699648-5 gnd rswk-swf Pluripotenz (DE-588)4420540-5 gnd rswk-swf Epigenetik (DE-588)7566079-9 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Embryonale Stammzelle (DE-588)4699648-5 s Pluripotenz (DE-588)4420540-5 s Epigenetik (DE-588)7566079-9 s DE-604 Erscheint auch als Online-Ausgabe urn:nbn:de:bvb:20-opus-84015 https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-84015 Resolving-System kostenfrei Volltext DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027079777&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Li, Xiaoli 1980- Functional analyses of ES cell pluripotency by inducible knockdown of the Polycomb group protein Pcgf6 Embryonale Stammzelle (DE-588)4699648-5 gnd Pluripotenz (DE-588)4420540-5 gnd Epigenetik (DE-588)7566079-9 gnd |
| subject_GND | (DE-588)4699648-5 (DE-588)4420540-5 (DE-588)7566079-9 (DE-588)4113937-9 |
| title | Functional analyses of ES cell pluripotency by inducible knockdown of the Polycomb group protein Pcgf6 |
| title_alt | Functionelle Analysen der ES-Zell-Pluripotenz durch induzierbaren Knockdown des Polycomb group Proteins Pcgf6 |
| title_auth | Functional analyses of ES cell pluripotency by inducible knockdown of the Polycomb group protein Pcgf6 |
| title_exact_search | Functional analyses of ES cell pluripotency by inducible knockdown of the Polycomb group protein Pcgf6 |
| title_full | Functional analyses of ES cell pluripotency by inducible knockdown of the Polycomb group protein Pcgf6 von Xiaoli Li |
| title_fullStr | Functional analyses of ES cell pluripotency by inducible knockdown of the Polycomb group protein Pcgf6 von Xiaoli Li |
| title_full_unstemmed | Functional analyses of ES cell pluripotency by inducible knockdown of the Polycomb group protein Pcgf6 von Xiaoli Li |
| title_short | Functional analyses of ES cell pluripotency by inducible knockdown of the Polycomb group protein Pcgf6 |
| title_sort | functional analyses of es cell pluripotency by inducible knockdown of the polycomb group protein pcgf6 |
| topic | Embryonale Stammzelle (DE-588)4699648-5 gnd Pluripotenz (DE-588)4420540-5 gnd Epigenetik (DE-588)7566079-9 gnd |
| topic_facet | Embryonale Stammzelle Pluripotenz Epigenetik Hochschulschrift |
| url | https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-84015 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027079777&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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