Maturation status of dendritic cells controls induction of tolerance versus immunity:
Gespeichert in:
| 1. Verfasser: | |
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| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
2011
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| Schlagworte: | |
| Online-Zugang: | Volltext http://d-nb.info/1045839469/34 Volltext Inhaltsverzeichnis |
| Beschreibung: | X, 90 Bö- graph. Darst. |
| Format: | Langzeitarchivierung gewährleistet, LZA |
Internformat
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| 245 | 1 | 0 | |a Maturation status of dendritic cells controls induction of tolerance versus immunity |c Stefanie Meierr |
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Datensatz im Suchindex
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| adam_text | TABLE OF CONTENTS
AUTHOR S DECLARATION III
LIST OF ABBREVIATIONS V
TABLE OF CONTENTS VIII
1 SUMMARY 1
2 ZUSAMMENFASSUNG 2
3 INTRODUCTION 4
3.1 THE IMMUNE SYSTEM 4
3.2 DENDRITIC CELLS 4
3.2.1 DC DEVELOPMENT 5
3.2.2 DC SUBSETS 6
3.2.2.1 LYMPHOID TISSUE DC 6
3.2.2.2 NON-LYMPHOID TISSUE DC 8
3.2.3 DC MATURATION 10
3.2.4 INDUCTION OF IMMUNITY BY DC 10
3.2.5 DC IN TOLERANCE INDUCTION 11
3.2.5.1 CENTRAL TOLERANCE 11
3.2.5.2 PERIPHERAL TOLERANCE 12
3.3 THE CD40 RECEPTOR 12
3.3.1 CD40 ON DENDRITIC CELLS 13
3.4 LMP1 AND CD40 14
3.5 THE FUSION PROTEIN LMP1/CD40 IS
4 AIMS OF THE THESIS 17
5 MATERIALS AND METHODS 18
5.1 MATERIALS 18
5.1.1 ANTIBODIES 18
5.1.2 CHEMICALS 20
5.1.3 CONSUMABLES 20
5.1.4 DEVICES 21
5.1.5 MEDIA AND SOILUTIONS 21
VIII
HTTP://D-NB.INFO/1046152629
5.1.6 . MOUSE STRAINS 24
5.1.7 PEPTIDES, PROTEINS AND OLIGONUCLEOTIDES 25
5.2 METHODS 25
5.2.1 CELLULAR AND IMMUNOLOGICAL METHODS 25
5.2.1.1 ADOPTIVE TRANSFER 25
5.2.1.2 CELL CULTURE 25
5.2.1.3 CFSE STAINING AND IN VIVO CYTOTOXICITY ASSAY 25
5.2.1.4 DETERMINATION OF CELL NUMBERS 26
5.2.1.5 FLOW CYTOMETRY - FLUORESCENCE-ACTIVATED CELL SORTING (FACS) 26
5.2.1.6 HARVESTING OF BLOOD AND ORGANS FROM MICE 27
5.2.1.7 GENERATION OF BONE MARROW CHIMERAS 29
5.2.1.8 DETECTION OF CYTOKINES VIA A CYTOMETRIC BEAD ARRAY SYSTEM 29
5.2.1.9 IG ISOTYPE ELLSAS 29
5.2.1.10 HISTOLOGY 29
5.2.1.11 MAGNETIC CELL SORTING (MACS) 30
5.2.1.12 DEPLETION OF GUT COMMENSAL MICROFLORA 31
5.2.2 MOLECULAR BIOLOGY METHODS 31
5.2.2.1 AGAROSE GEL ELECTROPHORESIS 31
5.2.2.2 DNA AND RNA ISOLATION AND PURIFICATION 31
5.2.2.3 MEASUREMENT OF NUCLEIC ACID CONCENTRATION 32
5.2.2.4 POLYMERASE CHAIN REACTION (PCR) 32
5.2.2.5 QUANTITATIVE PCR (QPCR) 34
5.2.2.6 WESTERN BLOTTING 36
5.2.3 STATISTICAL ANALYSIS.... 36
RESULTS 37
6.1 LMP1/CD40 EXPRESSION IN DC OF DC-LMP1/CD40 MICE 38
6.2 LOW ACTIVATION OF DC IN SPLEEN AND LYMPH NODES 39
6.3 OT-I T CELL PROLIFERATION AND KILLER ASSAYS 41
6.4 DC-LMP1/CD40 MICE DEVELOP SEVERE PATHOLOGY 43
6.4.1 WEIGHT LOSS AND REDUCED SURVIVAL 43
6.4.2 SPLENOMEGALY AND COLON SHORTENING 45
6.4.3 EXPANSION OF GRANULOCYTES IN THE SPLEEN AND BLOOD 45
6.4.4 INFILTRATIONS OF GRANULOCYTES IN THE COLON 47
6.4.5 SPLEEN HISTOLOGY 48
6.4.6 LANGERHANS CELLS 50
6.5 LOW T CELL ACTIVATION IN SPLEEN AND LYMPH NODES 51
6.6 AUTOANTIBODIES AND ELEVATED IGA AND IGM LEVELS IN THE BLOOD 53
6.7 INCREASE OF INFLAMMATORY CYTOKINES IN THE SERUM, SPLEEN AND COLON 54
6.8 CHARACTERIZATION OF COLONIC DC 56
6.8.1 REDUCED CD 103+DC 56
6.8.2 INCREASE OF IL-23 AND OTHER CYTOKINES IN COLONIC DC 57
6.8.3 CD 103+ DC IN THE SPLEEN 59
6.9 INTRINSIC DC ACTIVATION AND LACK OF COLONIC CD103+ DC DEVELOPMENT...
60
6.10 INCREASED T
H
17 CYTOKINES IN WHOLE COLON TISSUE 63
6.11 THE DC-LMP1/CD40 PHENOTYPE IS T/B-CELL DEPENDENT 64
6.12 THE DC-LMP1/CD40 PHENOTYPE IS DEPENDENT ON COMMENSAL BACTERIA 65
6.13 BM-DC STIMULATION 66
7 DISCUSSION 69
7.1 GENERATION OF DC-LMP1/CD40 MICE 69
7.2 IMPAIRED T CELL ACTIVATION, EXPANSION AND EFFECTOR FUNCTION IN THE
PERIPHERY 70
73 INFLAMMATORY CYTOKINES IN DC-LMP1/CD40 MICE 71
7.4 ROLE OF CD103+ DCS IN THE GUT 72
7.5 CONSTITUTIVE CD40 SIGNALING IN DCS DOES NOT INDUCE IMMUNE PATHOLOGY
WITHOUT MICROBIAL STIMULATION 73
7.6 DEVELOPMENT OF DISEASE IS T AND B CELL DEPENDENT 74
7.7 INCREASED SENSITIVITY FOR MICROBIAL MOLECULAR PATTERNS OF CD40
STIMULATED DCS 74
7.8 CONCLUSION 75
7.9 OUTLOOK 76
8 REFERENCES 78
9 CURRICULUM VITAE 89
10 ACKNOWLEDGEMENTS 90
X
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| spelling | Meier, Stefanie Verfasser aut Maturation status of dendritic cells controls induction of tolerance versus immunity Stefanie Meierr 2011 X, 90 Bö- graph. Darst. txt rdacontent n rdamedia nc rdacarrier München, Univ., Diss., 2012 Langzeitarchivierung gewährleistet, LZA (DE-588)4113937-9 Hochschulschrift gnd-content Erscheint auch als Online-Ausgabe urn:nbn:de:bvb:19-164263 https://nbn-resolving.org/urn:nbn:de:bvb:19-164263 Resolving-System kostenfrei Volltext http://d-nb.info/1045839469/34 Langzeitarchivierung Nationalbibliothek http://edoc.ub.uni-muenchen.de/16426/ Verlag kostenfrei Volltext DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027072419&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Meier, Stefanie Maturation status of dendritic cells controls induction of tolerance versus immunity |
| subject_GND | (DE-588)4113937-9 |
| title | Maturation status of dendritic cells controls induction of tolerance versus immunity |
| title_auth | Maturation status of dendritic cells controls induction of tolerance versus immunity |
| title_exact_search | Maturation status of dendritic cells controls induction of tolerance versus immunity |
| title_full | Maturation status of dendritic cells controls induction of tolerance versus immunity Stefanie Meierr |
| title_fullStr | Maturation status of dendritic cells controls induction of tolerance versus immunity Stefanie Meierr |
| title_full_unstemmed | Maturation status of dendritic cells controls induction of tolerance versus immunity Stefanie Meierr |
| title_short | Maturation status of dendritic cells controls induction of tolerance versus immunity |
| title_sort | maturation status of dendritic cells controls induction of tolerance versus immunity |
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