Liposomes in contact and interacting with silica nanoparticles: from decorated vesicles to internalized particles
Saved in:
| Main Author: | |
|---|---|
| Format: | Thesis Book |
| Language: | English |
| Published: |
Göttingen
Cuvillier
2013
|
| Edition: | 1. Aufl. |
| Subjects: | |
| Online Access: | Inhaltstext Inhaltsverzeichnis |
| Physical Description: | 180 S. Ill., graph. Darst. |
| ISBN: | 9783954045792 |
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Record in the Search Index
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TABLE OF CONTENT
TABLE OF CONTENT 20
1 INTRODUCTION 1
1.1 CONTEXT AND MOTIVATION 1
1.2 SILICA NANOPARTICLES 2
1.3 LIPOSOMES: DEFINITION AND PROPERTIES 2
1.4 INTERACTION IN MIXED SYSTEMS LIPOSOME/NANOPARTICLE 4
1.4.1 ROLE OF THE LIPID BILAYER PHASE BEHAVIOR 4
1.4.2 NATURE OF THE INTERACTION BETWEEN LIPOSOME AND NANOPARTICLES 6
1.4.2.1 VAN DER WAALS INTERACTION 6
1.4.2.2 ELECTROSTATIC INTERACTION 7
1.4.2.3 HYDRATION FORCES 8
1.4.2.4 HYDROPHOBIC INTERACTION 9
1.4.3 RESULTING STRUCTURES 10
1.4.3.1 SUPPORTED LIPID BILAYER (SLB) FORMATION AND PARTICLE
INTERNALIZATION. 10
1.4.3.2 DECORATED VESICLES 15
1.5 AIM AND OUTLINE 16
1.6 REFERENCES 18
2 METHODS 26
2.1 CHARACTERIZATION T ECHNIQUES 27
2.1.1 VISUAL INSPECTION 27
2.1.2 ZETA POTENTIAL MEASUREMENTS 27
2.1.2.1 THEORETICAL ASPECTS 27
2.1.2.2 MEASUREMENT 28
2.1.3 DYNAMIC AND STATIC LIGHT SCATTERING (DLS & SLS) 29
2.1.3.1 MEASUREMENT AND INSTRUMENT DESCRIPTION 29
2.1.3.2 STATIC LIGHT SCATTERING 32
2.1.3.3 CUMULANT ANALYSIS OF THE DLS DATA 32
2.1.4 SMALL ANGLE SCATTERING 33
2.1.4.1 THEORETICAL BACKGROUND 33
2.1.4.2 DIFFERENCES BETWEEN SAXS AND SANS 37
2.1.4.3 SMALL ANGLE NEUTRON SCATTERING (SANS) 38
2.1.4.4 SMALL ANGLE X-RAY SCATTERING (SAXS) 42
2.1.4.5 MODEL FREE ANALYSIS OF THE SMALL ANGLE SCATTERING DATA 43
2.1.4.5.1 BEHAVIOR AT THE LIMIT Q TENDING TO ZERO - GUINIER
APPROXIMATION .43
2.1.4.5.2 BEHAVIOR IN THE LARGE Q LIMIT - POROD LAW 43
2.1.4.5.3 SCATTERING INVARIANT 44
2.1.4.5.4 KRATKY-POROD PLOT 44
2.1.4.6 MODEL ANALYSIS OF THE SMALL-ANGLE SCATTERING DATA 45
2.1.4.6.1 FORM FACTORS 46
2.1.4.6.2 LOG-NORMAL SIZE DISTRIBUTION 47
2.1.5 FLUORESCENCE 48
2.1.5.1 FLUORESCENCE SPECTROSCOPY 48
2.1.5.2 FLUORESCENCE CORRELATION SPECTROSCOPY (FCS) 48
2.1.6 CRYOGENIC ELECTRON MICROSCOPY (CRYO-TEM) 51
2.1.6.1 PRINCIPLE 51
HTTP://D-NB.INFO/1045844322
2.1.6.2 BACKGROUND OF ELECTRON MICROSCOPY 52
2.1.6.2.1 ELECTRON-SPECIMEN INTERACTIONS 52
2.1.6.2.2 INSTRUMENTAL ASPECTS 53
2.1.6.2.3 SAMPLE PREPARATION FOR CRYO-TEM 55
2.1.6.3 OPERATING METHODS 56
2.1.7 DIFFERENTIAL SCANNING CALORIMETRY 57
2.1.7.1 PRINCIPLE 57
2.1.7.2 OPERATING METHODS 58
2.2 MATERIALS AND SAMPLE PREPARATION 58
2.2.1 SILICA NANOPARTICLES 58
2.2.1.1 BARE SILICA NANOPARTICLES 58
2.2.1.2 FUNCTIONALIZED SILICA NANOPARTICLES 59
2.2.2 LIPOSOME 60
2.2.2.1 LIPOSOMES COMPOSED OF ONE TYPE OF LIPID (DPPC AND DOPC) 60
2.2.2.2 LIPOSOMES COMPOSED OF A MIXTURE OF LIPIDS (DPPC/DMPA AND
DMPC/DMPA) 62
2.2.3 SAMPLE PREPARATION 64
2.3 REFERENCES 64
3 CONTROL OF STABILITY AND STRUCTURE OF GEL-PHASE LIPOSOMES BY MEANS
OF NANOPARTICLES 69
3.1 INTRODUCTION 69
3.2 CONCENTRATION RATIO AND SURFACE COVERAGE CALCULATION 70
3.2.1 CONCENTRATION RATIO 71
3.2.2 MAXIMUM SURFACE COVERAGE 72
3.3 RESULTS 74
3.3.1 STABILITY 74
3.3.1.1 VISUAL INSPECTION 74
3.3.1.2 DYNAMIC AND STATIC LIGHT SCATTERING (DLS & SLS) 76
3.3.1.3 DIFFERENTIAL SCANNING CALORIMETRY 79
3.3.1.4 ^-POTENTIAL 83
3.3.2 STRUCTURE 85
3.3.2.1 ^-POTENTIAL 85
3.3.2.2 CRYO-TEM 87
3.3.2.3 SMALL ANGLE NEUTRON SCATTERING (SANS) 90
3.3.3 INFLUENCE OF PH AND IONIC STRENGTH 93
3.3.3.1 STABILITY BEHAVIOR - EXPERIMENTAL RESULTS 93
3.3.3.2 STABILITY BEHAVIOR - DLVO THEORY 95
3.3.4 INFLUENCE OF THE VESICLE SURFACE CHARGES 99
3.3.4.1 VISUAL INSPECTION 100
3.3.4.2 DYNAMIC AND STATIC LIGHT SCATTERING (DLS & SLS) 101
3.3.4.3 DIFFERENTIAL SCANNING CALORIMETRY 103
3.3.4.4 SMALL ANGLE NEUTRON SCATTERING (SANS) 107
3.3.4.5 DLVO THEORY - TOTAL INTERACTION ENERGY CALCULATION 109
3.3.5 INFLUENCE OF THE NANOPARTICLE SURFACE CHARGES 111
3.3.5.1 STABILITY 112
3.3.5.2 STRUCTURE 115
3.4 DISCUSSION 117
3.5 CONCLUSION H 122
3.6 REFERENCES 123
4 INTERNALIZATION OF SILICA NANOPARTICLES WITHIN FLUID LIPOSOMES 127
4.1 INTRODUCTION 127
4.2 CONCENTRATION RATIO AND SURFACE COVERAGE CALCULATION 128
4.2.1 BILAYER VOLUME RATIO (F}) 129
4.3 RESULTS 132
4.3.1 STABILITY 132
4.3.1.1 VISUAL INSPECTION 132
4.3.1.2 DYNAMIC LIGHT SCATTERING (DLS) 134
4.3.1.3 STATIC LIGHT SCATTERING (SLS) 137
4.3.2 STRUCTURE 139
4.3.2.1 CRYOGENIC TRANSMISSION ELECTRON MICROSCOPY (CRYO-TEM) 139
4.3.2.2 SMALL ANGLE NEUTRON SCATTERING (SANS) 144
4.3.2.3 FLUORESCENCE CORRELATION SPECTROSCOPY (FCS) 148
4.3.3 INFLUENCE OF PH AND IONIC STRENGTH 151
4.3.3.1 VISUAL INSPECTION 151
4.3.3.2 DYNAMIC AND STATIC LIGHT SCATTERING 153
4.3.4 INFLUENCE OF VESICLE SIZE 155
4.3.4.1 SAMPLE PREPARATION AND CALCULATION OF THE CONCENTRATION RATIO
155
4.3.4.2 VISUAL INSPECTION 156
4.3.4.3 DYNAMIC AND STATIC LIGHT SCATTERING 156
4.4 DISCUSSION 159
4.5 CONCLUSION 164
4.6 REFERENCES 165
5 SUMMARY AND OUTLOOK 169
5.1 CONTROL OF STABILITY AND STRUCTURE OF GEL-PHASE LIPOSOMES BY MEANS
OF
NANOPARTICLES 169
5.2 INTERNALIZATION OF SILICA NANOPARTICLE WITHIN FLUID LIPOSOMES 171
5.3 CONCLUSION 174
5.4 OUTLOOK 174
APPENDIX 177
APPENDIX TO CHAPTER 3 177
APPENDIX TO CHAPTER 4 179 |
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| author | Michel, Raphaël |
| author_GND | (DE-588)1046659804 |
| author_facet | Michel, Raphaël |
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| bvnumber | BV041587808 |
| ctrlnum | (OCoLC)867874356 (DE-599)DNB1045844322 |
| dewey-full | 620.501572577 |
| dewey-hundreds | 600 - Technology (Applied sciences) |
| dewey-ones | 620 - Engineering and allied operations |
| dewey-raw | 620.501572577 |
| dewey-search | 620.501572577 |
| dewey-sort | 3620.501572577 |
| dewey-tens | 620 - Engineering and allied operations |
| discipline | Chemie / Pharmazie |
| edition | 1. Aufl. |
| format | Thesis Book |
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| spelling | Michel, Raphaël Verfasser (DE-588)1046659804 aut Liposomes in contact and interacting with silica nanoparticles from decorated vesicles to internalized particles Raphaël Michel 1. Aufl. Göttingen Cuvillier 2013 180 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Zugl.: Berlin, Techn. Univ., Diss., 2013 Nanopartikel (DE-588)4333369-2 gnd rswk-swf Wechselwirkung (DE-588)4064937-4 gnd rswk-swf Siliciumdioxid (DE-588)4077447-8 gnd rswk-swf Liposom (DE-588)4122262-3 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Liposom (DE-588)4122262-3 s Siliciumdioxid (DE-588)4077447-8 s Nanopartikel (DE-588)4333369-2 s Wechselwirkung (DE-588)4064937-4 s DE-604 X:MVB text/html http://deposit.dnb.de/cgi-bin/dokserv?id=4556579&prov=M&dok_var=1&dok_ext=htm Inhaltstext DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027032933&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Michel, Raphaël Liposomes in contact and interacting with silica nanoparticles from decorated vesicles to internalized particles Nanopartikel (DE-588)4333369-2 gnd Wechselwirkung (DE-588)4064937-4 gnd Siliciumdioxid (DE-588)4077447-8 gnd Liposom (DE-588)4122262-3 gnd |
| subject_GND | (DE-588)4333369-2 (DE-588)4064937-4 (DE-588)4077447-8 (DE-588)4122262-3 (DE-588)4113937-9 |
| title | Liposomes in contact and interacting with silica nanoparticles from decorated vesicles to internalized particles |
| title_auth | Liposomes in contact and interacting with silica nanoparticles from decorated vesicles to internalized particles |
| title_exact_search | Liposomes in contact and interacting with silica nanoparticles from decorated vesicles to internalized particles |
| title_full | Liposomes in contact and interacting with silica nanoparticles from decorated vesicles to internalized particles Raphaël Michel |
| title_fullStr | Liposomes in contact and interacting with silica nanoparticles from decorated vesicles to internalized particles Raphaël Michel |
| title_full_unstemmed | Liposomes in contact and interacting with silica nanoparticles from decorated vesicles to internalized particles Raphaël Michel |
| title_short | Liposomes in contact and interacting with silica nanoparticles |
| title_sort | liposomes in contact and interacting with silica nanoparticles from decorated vesicles to internalized particles |
| title_sub | from decorated vesicles to internalized particles |
| topic | Nanopartikel (DE-588)4333369-2 gnd Wechselwirkung (DE-588)4064937-4 gnd Siliciumdioxid (DE-588)4077447-8 gnd Liposom (DE-588)4122262-3 gnd |
| topic_facet | Nanopartikel Wechselwirkung Siliciumdioxid Liposom Hochschulschrift |
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| work_keys_str_mv | AT michelraphael liposomesincontactandinteractingwithsilicananoparticlesfromdecoratedvesiclestointernalizedparticles |