Sphingosine-1-phosphate regulated circulation of human B-cells: S1P receptor expression, signal transduction and migration
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| Main Author: | |
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| Format: | Thesis Book |
| Language: | English |
| Published: |
2012
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| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | 79 Bl. Ill., graph. Darst. 30 cm |
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Record in the Search Index
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| adam_text | IMAGE 1
CONTENTS
CONTENTS
1 SUMMARY 1
2 INTRODUCTION 2
2.1 THE IMMUNE SYSTEM 2
2.2 B CELLS 3
2.2.1 B CELL DEVELOPMENT IN THE BONE MARROW 3
2.2.2 B CELL MATURATION IN THE SPLEEN 4
2.3 SPHINGOSINE-L-PHOSPHATE 7
2.3.1 METABOLISM OF SIP 7
2.3.2 LYMPHOCYTE CIRCULATION AND THE VASCULAR SIP GRADIENT 8
2.3.3 EGRESS FROM THYMUS 10
2.3.4 EGRESS FROM LYMPH NODES 10
2.3.5 EGRESS FORM BONE MARROW 12
2.3.6 SIP RECEPTOR REGULATED POSITIONING AND EGRESS FROM SPLEEN 12
2.3.7 SIP RECEPTOR FUNCTIONS IN LYMPHOCYTES AND NON-LYMPHOCYTES 14
2.4 MULTIPLE SCLEROSIS 15
2.5 FINGOLIMOD (FTY720) 17
2.6 PRIMARY IMMUNODEFICIENCIES RELATED TO DEFECTIVE CYTOSKELETON
ORGANIZATION 18 2.7 OBJECTIVE 20
3 RESULTS 21
3.1 SIP RECEPTOR EXPRESSION IN B CELL SUBSETS 21
3.2 SIP DEPENDENCE O F PRIMARY HUMAN B CELL SUBSETS 25
3.3 SIP RECEPTOR SIGNALING IN LEUKEMIC B CELLS AND B CELL LINES 27
3.4S1P2, S1P4 AND FI-ARRESTIN2 REGULATE SIPL-INDUCED MIGRATION 30
3.5 B CELL ACTIVATION DOWN-REGULATES S I PI EXPRESSION AND SIGNALING 36
3.6 D0CK8, WASP AND LRBA ARE COMPONENTS O F THE SIPL-SIGNALING CASCADE
37 3.7 T CELL SUBSETS ARE MODULATED DURING FTY720 TREATMENT 40
3.8 FTY720 TREATMENT INFLUENCES THE RESPONSE O F CIRCULATING T CELLS TO
S I P 43
4 DISCUSSION 45
5 MATERIALS AND METHODS 53
5.1 PATIENT MATERIAL AND MICE 53
5.2 CELL BIOLOGICAL METHODS 53
5.2.1 CELL CULTURE 53
5.2.2 PREPARATION OF PRIMARY B CELLS 53
5.2.3 IMMUNOFLUORESCENCE 54
5.2.4 FLUORESCENCE MICROSCOPY 54
5.2.5 COLLAGEN COATING 55
5.2.6 TRANSFECTION AND LENTIVIRAL TRANSDUCTION 55
5.2.7 ACTIVATION OF PRIMARY B CELLS 55
5.2.8 FLOW CYTOMETRY AND CELL SORTING 55
5.2.9 PREPARATION OF SIP AND FTY720P SOLUTIONS 56
5.2.10 TRANSWELL MIGRATION ASSAY 56
5.3 PROTEIN BIOCHEMISTRY 57
5.3.1 CELL LYSIS 57
5.3.2 IMMUNOBLOTTING 57
5.4 MOLECULAR BIOLOGICAL METHODS 57
5.4.1 TRANSFORMATION OF COMPETENT E.COLI 57
5.4.2 PLASMID PREPARATIONS 58
5.4.3 ENZYMATIC TREATMENT OF DNA 58
5.4.4 POLYMERASE CHAIN REACTION (PCR) 58
5.4.5 DNA SEQUENCING 59
HTTP://D-NB.INFO/1036248666
IMAGE 2
CONTENTS
5.4.6 RNA PREPARATION AND CDNA-SYNTHESIS 59
5.4.7 QUANTITATIVE POLYMERASE CHAIN REACTION (QPCR) 60
5.4.8 PRIMER AND PROBES 60
5.5 VECTORS 61
5.5.1 VECTOR PNL-CEF-SLP(R)EG/R/B-FP 6 1
5.5.2 LINKER FOR SIPL-EGFP FUSION 6 1
5.5.3 SEQUENCE ANALYSIS 62
5.6 STATISTICAL ANALYSIS 62
6 APPENDIX 63
6.1 REFERENCES 63
6.2 LIST O F ABBREVIATIONS 75
6.3 ACKNOWLEDGEMENTS 79
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| discipline | Biologie Medizin |
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| illustrated | Illustrated |
| indexdate | 2024-07-10T01:00:09Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-027028806 |
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| physical | 79 Bl. Ill., graph. Darst. 30 cm |
| publishDate | 2012 |
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| spelling | Sic, Heiko Verfasser aut Sphingosine-1-phosphate regulated circulation of human B-cells S1P receptor expression, signal transduction and migration vorgelegt von Heiko Sic 2012 79 Bl. Ill., graph. Darst. 30 cm txt rdacontent n rdamedia nc rdacarrier Freiburg (Breisgau), Univ., Diss., 2013 (DE-588)4113937-9 Hochschulschrift gnd-content DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027028806&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Sic, Heiko Sphingosine-1-phosphate regulated circulation of human B-cells S1P receptor expression, signal transduction and migration |
| subject_GND | (DE-588)4113937-9 |
| title | Sphingosine-1-phosphate regulated circulation of human B-cells S1P receptor expression, signal transduction and migration |
| title_auth | Sphingosine-1-phosphate regulated circulation of human B-cells S1P receptor expression, signal transduction and migration |
| title_exact_search | Sphingosine-1-phosphate regulated circulation of human B-cells S1P receptor expression, signal transduction and migration |
| title_full | Sphingosine-1-phosphate regulated circulation of human B-cells S1P receptor expression, signal transduction and migration vorgelegt von Heiko Sic |
| title_fullStr | Sphingosine-1-phosphate regulated circulation of human B-cells S1P receptor expression, signal transduction and migration vorgelegt von Heiko Sic |
| title_full_unstemmed | Sphingosine-1-phosphate regulated circulation of human B-cells S1P receptor expression, signal transduction and migration vorgelegt von Heiko Sic |
| title_short | Sphingosine-1-phosphate regulated circulation of human B-cells |
| title_sort | sphingosine 1 phosphate regulated circulation of human b cells s1p receptor expression signal transduction and migration |
| title_sub | S1P receptor expression, signal transduction and migration |
| topic_facet | Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027028806&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT sicheiko sphingosine1phosphateregulatedcirculationofhumanbcellss1preceptorexpressionsignaltransductionandmigration |