Contribution of cathepsin proteases to cellular protein homeostasis:
Saved in:
| Main Author: | |
|---|---|
| Format: | Thesis Book |
| Language: | English |
| Published: |
2013
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| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Item Description: | Enth. außerdem 5 Sonderabdr. aus verschiedenen Zeitschr. |
| Physical Description: | 72, [141] S. Ill., graph. Darst. 30 cm |
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| adam_text | TABLE OF CONTENTS
TABLE OF CONTENTS
1. ZUSAMMENFASSUNG 1
2. ABSTRACT 3
3. INTRODUCTION 4
3.1. PROTEOLYSIS 4
3.2. THE FAMILY OF CATHEPSIN PROTEASES 5
3.3. CATHEPSIN SYNTHESIS, PROCESSING,
TRAFFICKING AND
REGULATION 5
3.4. THE ROLE OF CATHEPSINS B AND L 7
3.5. CATHEPSINS B AND
L - STRUCTURE AND SPECIFICITY 9
3.6. MASS SPECTROMETRY-BASED QUALITATIVE
AND QUANTITATIVE PROTEOMICS 11
3.7. TERMINOMIC TECHNIQUES 13
4. AIM OF THE PRESENT STUDY 16
5. SUMMARY OF RESULTS 17
5.1. CONTRIBUTION
OF CATHEPSINS B AND
L TO SECRETOME
COMPOSITION OF MEFS 17
5.1.1. GENERATION AND CHARACTERIZATION OF CELL LINES 17
5.1.2. EFFECT OF CATHEPSIN DEPLETION ON PROTEIN ABUNDANCE 18
R
5.1.3. BIOLOGICAL PROCESSES AFFECTED BY CATHEPSIN B AND CATHEPSIN L
DEPLETION 20
5.1.4. IMPACT OF CATHEPSINS B AND L ON PROTEIN ABUNDANCE OF PERIOSTIN 22
5.1.5. IDENTIFICATION OF CLEAVAGE EVENTS DEPENDENT ON CATHEPSINS B OR L
BY TAILS 24
5.1.6. CATHEPSIN L, AND TO A LESSER EXTENT CATHEPSIN B, AFFECT MMP-2
PROTEIN LEVELS, RESULTING IN
SECONDARY DOWNSTREAM EFFECTS 27
5.2. IMPACT OF CATHEPSINS
B AND L ON
THE MURINE SKIN PROTEOME AND
DEGRADOME 29
5.2.1. CATHEPSIN B AND CATHEPSIN L DEPLETION AFFECT PROTEIN ABUNDANCE IN
MURINE SKIN 29
5.2.2. PERIOSTIN ACCUMULATES IN CATHEPSIN L DEFICIENT SKIN 31
5.2.3. CATHEPSIN L, AND TO A LESSER EXTENT CATHEPSIN B, DEPLETION
AFFECTS PROTEIN ABUNDANCE OF
NUMEROUS PROTEASES AND PROTEASE INHIBITORS 33
5.2.4. N-TERMINAL CLEAVAGE SITE ANALYSIS OF CATHEPSIN BAND CATHEPSIN L
DEFICIENT SKIN 35
5.2.5. LACK OF CATHEPSIN L AFFECTS NOTCH AND WNT SIGNALING 37
II
HTTP://D-NB.INFO/1044614609
TABLE OF CONTENTS
6. DISCUSSION 40
6.1. CHARACTERIZATION OF CATHEPSIN DEFICIENT MEFS
REVEALS ACCUMULATION OF
LYSOSOME-LIKE ORGANELLES 40
6.2. COOPERATIVE FUNCTIONALITY OF CATHEPSINS
B AND L 41
6.3. CATHEPSIN B AND
CATHEPSIN L MOLD
THE EXTRACELLULAR MATRIX
VIA DIRECT AND
INDIRECT
MECHANISMS 42
6.4. CATHEPSINS B AND L AS IMPORTANT
NODES IN THE PROTEOLYTIC NETWORK 44
6.5. CATHEPSIN DEPLETION AFFECTS A MULTITUDE
OF PROTEOLYTIC PROCESSING SITES 45
6.6. CATHEPSIN L IN
EPIDERMAL HOMEOSTASIS
AND HAIR CYCLE
REGULATION 47
7. PERSPECTIVES 50
8. ABBREVIATIONS 51
9. REFERENCES 53
10. CURRICULUM VITAE 67
11. RESEARCH PRODUCTIVITY 68
11.1. PUBLICATIONS 68
11.2. PRESENTATIONS 69
12. DANKSAGUNG , 71
EIDESSTATTLICHE ERKLARUNG
APPENDIX 1
APPENDIX 2
APPENDIX 3
APPENDIX 4
APPENDIX 5
III
|
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| author_facet | Tholen, Stefan 1980- |
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| bvnumber | BV041564776 |
| ctrlnum | (OCoLC)865733000 (DE-599)DNB1044614609 |
| dewey-full | 572.648 |
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| dewey-ones | 572 - Biochemistry |
| dewey-raw | 572.648 |
| dewey-search | 572.648 |
| dewey-sort | 3572.648 |
| dewey-tens | 570 - Biology |
| discipline | Biologie |
| format | Thesis Book |
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| indexdate | 2024-07-10T00:59:43Z |
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| language | English |
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| physical | 72, [141] S. Ill., graph. Darst. 30 cm |
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| spelling | Tholen, Stefan 1980- Verfasser (DE-588)1044614897 aut Contribution of cathepsin proteases to cellular protein homeostasis vorgelegt von Stefan Tholen 2013 72, [141] S. Ill., graph. Darst. 30 cm txt rdacontent n rdamedia nc rdacarrier Enth. außerdem 5 Sonderabdr. aus verschiedenen Zeitschr. Freiburg (Breisgau), Univ., Diss., 2013 (DE-588)4113937-9 Hochschulschrift gnd-content DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027010308&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Tholen, Stefan 1980- Contribution of cathepsin proteases to cellular protein homeostasis |
| subject_GND | (DE-588)4113937-9 |
| title | Contribution of cathepsin proteases to cellular protein homeostasis |
| title_auth | Contribution of cathepsin proteases to cellular protein homeostasis |
| title_exact_search | Contribution of cathepsin proteases to cellular protein homeostasis |
| title_full | Contribution of cathepsin proteases to cellular protein homeostasis vorgelegt von Stefan Tholen |
| title_fullStr | Contribution of cathepsin proteases to cellular protein homeostasis vorgelegt von Stefan Tholen |
| title_full_unstemmed | Contribution of cathepsin proteases to cellular protein homeostasis vorgelegt von Stefan Tholen |
| title_short | Contribution of cathepsin proteases to cellular protein homeostasis |
| title_sort | contribution of cathepsin proteases to cellular protein homeostasis |
| topic_facet | Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027010308&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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