Dual-channel STED microscope with pulsed interleaved excitation:
Saved in:
| Main Author: | |
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| Format: | Thesis Book |
| Language: | English |
| Published: |
2013
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| Subjects: | |
| Online Access: | Volltext https://nbn-resolving.org/urn:nbn:de:bvb:91-diss-20131127-1174212-0-8 Inhaltsverzeichnis |
| Physical Description: | VII, 156 S. Ill., graph. Darst. |
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MARC
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| 245 | 1 | 0 | |a Dual-channel STED microscope with pulsed interleaved excitation |c Heinrich Ernst Paul Grabmayr |
| 264 | 1 | |c 2013 | |
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Record in the Search Index
| _version_ | 1804151657536159744 |
|---|---|
| adam_text | CONTENTS
SUMMARY I
1 INTRODUCTION 1
2 OPTICAL MICROSCOPY OF BIOMATERIALS - CONCEPTS 3
2.1 WIDE FIELD MICROSCOPE 4
2.1.1 RESOLUTION CRITERIA 8
2.1.2 DEPTH OF FIELD 11
2.1.3 CONTRAST 11
2.2 PHOTOPHYSICS OF FLUOROPHORES 12
2.3 CONFOCAL MICROSCOPE 16
2.3.1 RESOLUTION 17
2.3.2 DEPTH OF FIELD 19
2.4 STED MICROSCOPE 19
2.4.1 DEPLETION PATTERN AND POLARIZATION 22
2.4.2 RESOLUTION 26
2.4.3 IMPACT OF STED MICROSCOPY ON THE LIFE SCIENCES .... 28
2.4.4 RECENT DEVELOPMENTS IN STED TECHNOLOGY 29
3 OPTICAL MICROSCOPY OF BIOMATERIALS - TECHNICAL ASPECTS . . 31
3.1 STED SETUP DESIGN 31
3.1.1 SPECTRAL DESIGN 31
3.1.2 TIMING DESIGN 39
3.1.3 BEAM PATHS 41
3.1.4 CONTROL AND ACQUISITION ELECTRONICS 45
3.2 CHARACTERIZATION 46
3.2.1 STED PULSE TIMING 46
V
HTTP://D-NB.INFO/1047943360
CONTENTS
3.2.2 POLARIZATION DEPENDENCE OF THE DONUT 50
3.2.3 TIME-GATING 52
3.2.4 CHROMATIC ABERRATION 60
3.2.5 RESOLUTION SCALING 63
3.2.6 DETECTION EFFICIENCY 70
3.2.7 ISSUES TO IMPROVE ON 72
4 INVESTIGATIONS 75
4.1 DNA BASED APPROACHES TO STED MICROSCOPY 75
4.1.1 DNA FILAMENTS 76
4.1.2 INFINITE IMAGING VIA DNA-PAINT 78
4.1.2.1 BLEACH RATES 80
4.1.2.2 BACKGROUND AND CONTRAST 84
4.1.2.3 IMAGER SURFACE INTERACTION 87
4.1.2.4 SYNOPSIS : 89
4.1.2.5 OUTLOOK 91
4.2 STED MICROSCOPY AND CYTOSKELETAL PROTEINS 92
4.2.1 LEGIONELLA 92
4.2.2 A-SYNUCLEIN 93
4.2.3 KERATIN 103
4.3 STED-FRET 104
4.3.1 FRET 105
4.3.2 IMPLEMENTATION 105
4.3.3 PREREQUISITES FOR DOUBLESTED-FRET 109
APPENDIX ILL
A PROTOCOLS 112
A.L FLOW CHAMBER IMMOBILIZATION PROTOCOL 112
A.2 GOLD BEAD PREPARATION 112
A.3 MOWIOL ANTIFADE FIXATION PROTOCOL 112
A.4 STED PULSE TIMING 113
A.5 CHROMATIC ABERRATION 113
A.6 RESOLUTION SCALING 114
A.7 DETECTION EFFICIENCY 114
A.8 PAINT 114
CONTENTS
A.9 STED-FRET ON ACTIN NETWORKS 115
A.10 DNA FILAMENTS 115
B LIST OF DYES THAT HAVE BEEN USED IN THE SYSTEM 115
C ALIGNMENT 116
C.L WORKFLOW FOR
DAILY ALIGNMENT 116
C.2 WORKFLOW FOR
BIG ALIGNMENT 118
C.3 WORKFLOW FOR
ALIGNMENT FROM SCRATCH 119
D HARDWARE 125
D.L INTENSITY PHOTODIODE 125
D.2 CIRCULAR POLARIZATION ANALYZER 126
D.3 TIMEGATING DEVICE 126
D.4 COUNTER 128
D.5 SERVO MICROCONTROLLER 128
E SOFTWARE 130
E.L ACQUISITION SOFTWARE * USAGE 130
E.L.L MICROSTAGE MOVEMENT 130
E.1.2 GENERAL DESCRIPTION OF STEDACQUIRATOR .... 131
E.1.3 MODES OF ACQUISITION 134
E.2 ACQUISITION SOFTWARE * DESIGN 134
E.2.1 SOME LABVIEW CONSTRUCTS USED 134
E.2.2 STEDACQUIRATOR. VI 135
E.2.3 MICROSTAGESTEUERUNG.VI 137
E.3 IMAGEJ PLUGINS 137
E.4 SPECTRAVIEWER AND OTHER MATLAB SCRIPTS 137
E.5 OME-TIFF DLL 137
F DEFINITIONS 139
A BIBLIOGRAPHY 145
INDEX 155
|
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| author_sort | Grabmayr, Heinrich Ernst Paul |
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| dewey-ones | 500 - Natural sciences and mathematics |
| dewey-raw | 500 |
| dewey-search | 500 |
| dewey-sort | 3500 |
| dewey-tens | 500 - Natural sciences and mathematics |
| discipline | Allgemeine Naturwissenschaft Physik Biologie |
| format | Thesis Book |
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| indexdate | 2024-07-10T00:58:18Z |
| institution | BVB |
| language | English |
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| oclc_num | 867225762 |
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| physical | VII, 156 S. Ill., graph. Darst. |
| psigel | ebook |
| publishDate | 2013 |
| publishDateSearch | 2013 |
| publishDateSort | 2013 |
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| spelling | Grabmayr, Heinrich Ernst Paul Verfasser aut Dual-channel STED microscope with pulsed interleaved excitation Heinrich Ernst Paul Grabmayr 2013 VII, 156 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier München, Techn. Univ., Diss., 2013 (DE-588)4113937-9 Hochschulschrift gnd-content Erscheint auch als Online-Ausgabe urn:nbn:de:bvb:91-diss-20131127-1174212-0-8 http://mediatum.ub.tum.de/node?id=1174212 Verlag kostenfrei Volltext https://nbn-resolving.org/urn:nbn:de:bvb:91-diss-20131127-1174212-0-8 Resolving-System DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=026995238&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Grabmayr, Heinrich Ernst Paul Dual-channel STED microscope with pulsed interleaved excitation |
| subject_GND | (DE-588)4113937-9 |
| title | Dual-channel STED microscope with pulsed interleaved excitation |
| title_auth | Dual-channel STED microscope with pulsed interleaved excitation |
| title_exact_search | Dual-channel STED microscope with pulsed interleaved excitation |
| title_full | Dual-channel STED microscope with pulsed interleaved excitation Heinrich Ernst Paul Grabmayr |
| title_fullStr | Dual-channel STED microscope with pulsed interleaved excitation Heinrich Ernst Paul Grabmayr |
| title_full_unstemmed | Dual-channel STED microscope with pulsed interleaved excitation Heinrich Ernst Paul Grabmayr |
| title_short | Dual-channel STED microscope with pulsed interleaved excitation |
| title_sort | dual channel sted microscope with pulsed interleaved excitation |
| topic_facet | Hochschulschrift |
| url | http://mediatum.ub.tum.de/node?id=1174212 https://nbn-resolving.org/urn:nbn:de:bvb:91-diss-20131127-1174212-0-8 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=026995238&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT grabmayrheinrichernstpaul dualchannelstedmicroscopewithpulsedinterleavedexcitation |