Cell-cycle control during root growth and development in Arabidopsis thaliana:
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| Format: | Thesis Book |
| Language: | English German |
| Published: |
2012
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| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Item Description: | Zsfassung in dt. und engl. Sprache |
| Physical Description: | IX, 152 S. Ill., graph. Darst. |
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| 245 | 1 | 0 | |a Cell-cycle control during root growth and development in Arabidopsis thaliana |c vorgelegt von Annika Katharina Weimer |
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| 502 | |a Köln, Univ., Diss., 2012 | ||
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| adam_text | Titel: Cell-cycle control during root growth and development in Arabidopsis thaliana
Autor: Weimer, Annika Katharina
Jahr: 2012
Contents
Contents....................................................................................................................................I
Abstract....................................................................................................................................V
Kurzzusammenfassung.........................................................................................................VI
Publications..........................................................................................................................VIII
Nomenclature.........................................................................................................................IX
1. Introduction.,
1.1. The cell cycle................................................................................................................1
1.2. Checkpoints..................................................................................................................2
1.3. The plant cell cycle......................................................................................................3
1.4. Cell-cycle regulators in plants....................................................................................4
1.4.1. Cyclin-dependent kinases........................................................................................4
1.4.1.1. CDKA;1.............................................................................................................4
1.4.1.2. The plant specific family of CDKBs...................................................................5
1.4.1.2.1. CDKB1........................................................................................................6
1.4.1.2.2. CDKB2........................................................................................................6
1.4.1.3. Cdk-activating kinases......................................................................................7
1.4.2. Cyclins.....................................................................................................................9
1.4.3. Inhibitors................................................................................................................11
1.4.4. Retinoblastoma-related 1.......................................................................................12
1.5. Posttranslatjonal modifications ot Cdks..................................................................14
1.5.1. T-loop phosphorylation..........................................................................................15
1.5.2. P-loop phosphorylation..........................................................................................15
1.5.2.1. Wee1...............................................................................................................16
1.5.2.2. The curious case of the plant homologue for Cdc25.......................................17
1.6. Developmental control of cell division..........................................................___..18
1.6.1. The Arabidopsis root is organized into different cell layers....................................18
1.6.2. SCARECROW regulates CYCD6;1 in asymmetric cell divisions in the root..........20
1.6.3. FOURLIPS/MYB88 regulate Cdks and cyclins in the stomatal lineage.................21
1.7. Genotoxic stress and cell cycle regulation_______.............................................22
1.7.1. The two major repair pathways in plants: HR and NHEJ.......................................24
1.7.2. Mechanisms of genotoxic stress inducers.............................................................25
2. Objectives..........................................................................................................................28
3. Results............................................................................................................................_.29
3.1. Control of asymmetric cell divisions in AtabUopsIs thallana...............................29
3.1.1. Involvement of CDKA;1 in asymmetric cell divisions.............................................29
3.1.1.1. Weak loss-of-function cdka;1 alleles have shorter roots than wild-type plants29
3.1.1.2. Asymmetric divisions are delayed in weak loss-of-function cdka;1 alleles......30
3.1.1.3. Casparian strip formation is not disturbed in weak cdka;1 mutants................34
3.1.1.4. Failure of asymmetric cell divisions in cdka;1 null mutants.............................35
3.1.1.5. Time-course of cortex-endodermis differentiation in weak cdka;1 alleles.......36
3.1.2. Involvement of CDKBIs in asymmetric cell divisions............................................37
3.1.2.1. CDKB1 function is partially redundant to CDKA;1...........................................37
3.1.2.2. cdkbls have a weak mutant phenotype for asymmetric cell divisions............39
3.1.3. Involvement of CYCLIN D6;1 in asymmetric cell divisions....................................41
3.1.3.1. CYCD6;1 :GUS marks additional cell files in weak cdka;1 alleles....................41
3.1.3.2. cycd6;1 displays a weak delay in asymmetric cell divisions in the root...........42
3.1.4. The transcription factor RBR1 links cell proliferation with differentiation...............43
3.1.4.1. rbrl rescues asymmetric divisions in co*a,t mutants.....................................43
3.1.4.2. CEID division in rbrl is faster at permissive temperatures..............................45
3.1.4.3. Less phosphorylation of RBR1 in weak cdka;1 alleles....................................45
3.1.4.4. RBR1 regulates cell differentiation genes of the SHR/SCR pathway..............46
3.2. The role of B1-type cyclins in the DNA damage response....................................51
3.2.1. B1-type cyclins and cell cycle regulation in the root..............................................51
3.2.2. Analysis of the growth of B1 -type cyclin mutants...................................................51
3.2.2.1. Global phenotype of B1-type cyclin mutants...................................................51
3.2.2.2. Root growth performance of B1-type cyclin mutants.......................................53
3.2.3. Analysis of hypersensitivity of B1 - type cyclins to genotoxic stress.......................54
3.2.3.1. B1-type cyclin mutants are not hypersensitive to hydroxyurea.......................54
3.2.3.2. B1-type cyclin mutants are not hypersensitive to bleomycin...........................56
3.2.3.3. B1-type cyclin mutants are sensitive to cisplatin.............................................57
3.2.3.4. CYCB1 ;1 is upregulated after cisplatin treatment...........................................59
3.2.3.5. The double mutant cdkb1;1 cdkb1]2 is hypersensitive to cisplatin..................60
3.2.4. Involvement of B1-type cyclins in the DNA repair by HR.......................................61
3.2.5. CYCB1 s are involved in mitotic chromosome segregation....................................63
3.3. WEE1 under DNA damage conditions........................................................._____65
3.3.1. WEE1 is not hypersensitive to bleomycin..............................................................65
3.3.2. WEE1 action on CDKBs........................................................................................66
3.3.3. WEE1 action upstream of T-loop activation in CDKA;1.........................................68
3.3.4. Phosphorylation-independent function for WEE1 after genotoxic stress...............74
3.3.5. EMS suppressor screen of wsef mutants.............................................................75
3.3.6. Tyr4-phosphorylation of CDKA;1...........................................................................76
4. Discussion.........................................................................................................................77
4.1. Control of asymmetric cell divisions in AraUdopsis..............................................77
4.1.1. Asymmetric cell divisions in the root depend on CDKA;1/RBR1...........................77
4.1.2. Asymmetric divisions in the stomatal lineage follow the same mechanism than in
roots.......................................................................................................................81
4.1.3. Species-specific differences of Cdk1 function in asymmetric cell divisions...........83
4.2. Function of CDKB1/CYCB1 complexes in the genotoxic stress response_____83
4.3. Function of WEE1 in cell cycle control upon DNA damage...................................91
5. Materials......______......._.................................................................................................97
5.1. Vectors and constructs.............................................................................................97
5.2. Plant material.................................................„...........................................................99
5.2.1. Transgenic plants...................................................................................................99
5.2.2. T-DNA insertion lines...........................................................................................101
5.2.3. Crosses................................................................................................................103
5.3. Primers........................................„............................................................................105
.112
6.1. Plant methods___.........................._........................................................................112
6.2. Cytology and Microscopy________________.........................................................115
6.3. Nucleic add work___........_____________..............................................................116
6 A Protein work________________________..............................................................118
6.5. Microbiological work and cloning___________.....................................................123
7. Appendix..........................................................................................................................127
7.1. Amino acids..........................................................................................................._ 127
7.2. Abbreviations......................................................_______________________128
8. References.........................................................................._______............................133
9. Figures and Tables.................................................................._________..................147
10. Danksagung.............................................................................................................___150
11. Erklarung.......................................................................................................................151
12. Curriculum vitae............................................................................................................152
|
| any_adam_object | 1 |
| author | Weimer, Annika Katharina 1983- |
| author_GND | (DE-588)1044803126 |
| author_facet | Weimer, Annika Katharina 1983- |
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| dewey-ones | 575 - Specific parts of & systems in plants |
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| dewey-tens | 570 - Biology |
| discipline | Biologie |
| format | Thesis Book |
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| spelling | Weimer, Annika Katharina 1983- Verfasser (DE-588)1044803126 aut Cell-cycle control during root growth and development in Arabidopsis thaliana vorgelegt von Annika Katharina Weimer 2012 IX, 152 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Zsfassung in dt. und engl. Sprache Köln, Univ., Diss., 2012 (DE-588)4113937-9 Hochschulschrift gnd-content HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=026910741&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Weimer, Annika Katharina 1983- Cell-cycle control during root growth and development in Arabidopsis thaliana |
| subject_GND | (DE-588)4113937-9 |
| title | Cell-cycle control during root growth and development in Arabidopsis thaliana |
| title_auth | Cell-cycle control during root growth and development in Arabidopsis thaliana |
| title_exact_search | Cell-cycle control during root growth and development in Arabidopsis thaliana |
| title_full | Cell-cycle control during root growth and development in Arabidopsis thaliana vorgelegt von Annika Katharina Weimer |
| title_fullStr | Cell-cycle control during root growth and development in Arabidopsis thaliana vorgelegt von Annika Katharina Weimer |
| title_full_unstemmed | Cell-cycle control during root growth and development in Arabidopsis thaliana vorgelegt von Annika Katharina Weimer |
| title_short | Cell-cycle control during root growth and development in Arabidopsis thaliana |
| title_sort | cell cycle control during root growth and development in arabidopsis thaliana |
| topic_facet | Hochschulschrift |
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