Chemical and enzymatic synthesis of N-glycans for application on microarrays:
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| Format: | Thesis Book |
| Language: | English |
| Published: |
Bayreuth
Universitätsbibl.
2013
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| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | 163 S. graph. Darst. |
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Record in the Search Index
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| adam_text | TABLE OF CONTENT
TABLE OF CONTENT
ABBREVIATIONS 7
1 INTRODUCTION 11
1.1 N-GLYCANS - STRUCTURE AND FUNCTIONS 11
1.2 INVESTIGATION OF CARBOHYDRATE - PROTEIN INTERACTIONS IS
1.3 CHEMO-ENZYMATIC SYNTHESIS OF N-GLYCANS 21
2 OBJECTIVES 29
3 SYNTHESIS OF CORE FUCOSYLATED N-GLYCANS 30
3.1 SYNTHESIS OF CORE TRISACCHARIDE 31 30
3.2 SYNTHESIS OFBIANTENNARY CORE-FUCOSYLATED N-GLYCANS 34
4 ENZYMATIC CORE FUCOSYLATION 39
5 CHEMICAL SYNTHESIS OF A LIBRARY OF N-GLYCANS 46
5.1 SYNTHESIS OFBIANTENNARY N-GLYCAN 62 47
5.2 SYNTHESIS OF 2,6-BRANCHED N-GLYCAN 63 51
5.3 SYNTHESIS OF 2,4-BRANCHED N-GLYCAN 67 52
5.4 SYNTHESIS OF THE TETRAANTENNARY N-GLYCAN 68 53
5.5 INTRODUCTION OF A BISECTING GLCNAC TO MULTIANTENNARY N-GLYCANS 54
5.6 SELECTIVE DEPROTECTION OF N-GLYCANS TO N-GLYCAN AZIDES 57
6 MICROARRAY PREPARATION 63
7 BINDING ASSAYS 67
7.1 SAMBUCUS NIGRA AGGLUTININ (SNA) 67
7.2 RICINUS COMMUNIS AGGLUTININ 1 (RCA-I) 68
7.3 CONCANAVALIN A (CON A) 69
7.4 WHEAT GERM AGGLUTININ (WGA) 70
7.5 WISTERIAJLORIBUNDA LECTIN (WFA) 71
7.6 DATURA STRAMONIUM AGGLUTININ (DSA) 72
7.7 PHASEOLUS VULGARIS ERYTHROOAGGLUTININ (PHA-E) 72
7.8 PHASEOLUS VULGARIS LEUKOAGGLUTININ (PHA-L) 75
8 SUMMARY 79
9 ZUSAMMENFASSUNG 83
10 EXPERIMENTAL SECTION 87
5
HTTP://D-NB.INFO/1045062715
TABLE OF CONTENT
10.1 GENERAL METHODS 87
10.2 EXPERIMENTS TO CHAPTER 3.1 88
10.3 EXPERIMENTS TO CHAPTER 3.2 93
10.4 EXPERIMENTS TO CHAPTER 4 97
10.5 EXPERIMENTS TO CHAPTER 5.1 99
10.6 EXPERIMENTS TO CHAPTER 5.2 105
10.7 EXPERIMENTS TO CHAPTER 5.3 107
10.8 EXPERIMENTS TO CHAPTER 5.4 110
10.9 EXPERIMENTS TO CHAPTER 5.5 112
10.10 EXPERIMENTS TO CHAPTER 5.6 118
10.12 EXPERIMENTS TO CHAPTER 6 132
10.13 STANDARD PROTOCOL FOR MICRORRAY VALIDATIONS 135
11 APPENDIX 138
12 ACKNOWLEDGEMENTS ISS
13 REFERENCES 157
6
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| dewey-sort | 3547.78 |
| dewey-tens | 540 - Chemistry and allied sciences |
| discipline | Chemie / Pharmazie |
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| spelling | Hoffman, Joanna Verfasser aut Chemical and enzymatic synthesis of N-glycans for application on microarrays by Joanna Hoffman Bayreuth Universitätsbibl. 2013 163 S. graph. Darst. txt rdacontent n rdamedia nc rdacarrier Bayreuth, Univ., Diss., 2013 Microarray (DE-588)4544227-7 gnd rswk-swf Polysaccharide (DE-588)4131871-7 gnd rswk-swf Chemische Synthese (DE-588)4133806-6 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Polysaccharide (DE-588)4131871-7 s Chemische Synthese (DE-588)4133806-6 s Microarray (DE-588)4544227-7 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=026884545&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Hoffman, Joanna Chemical and enzymatic synthesis of N-glycans for application on microarrays Microarray (DE-588)4544227-7 gnd Polysaccharide (DE-588)4131871-7 gnd Chemische Synthese (DE-588)4133806-6 gnd |
| subject_GND | (DE-588)4544227-7 (DE-588)4131871-7 (DE-588)4133806-6 (DE-588)4113937-9 |
| title | Chemical and enzymatic synthesis of N-glycans for application on microarrays |
| title_auth | Chemical and enzymatic synthesis of N-glycans for application on microarrays |
| title_exact_search | Chemical and enzymatic synthesis of N-glycans for application on microarrays |
| title_full | Chemical and enzymatic synthesis of N-glycans for application on microarrays by Joanna Hoffman |
| title_fullStr | Chemical and enzymatic synthesis of N-glycans for application on microarrays by Joanna Hoffman |
| title_full_unstemmed | Chemical and enzymatic synthesis of N-glycans for application on microarrays by Joanna Hoffman |
| title_short | Chemical and enzymatic synthesis of N-glycans for application on microarrays |
| title_sort | chemical and enzymatic synthesis of n glycans for application on microarrays |
| topic | Microarray (DE-588)4544227-7 gnd Polysaccharide (DE-588)4131871-7 gnd Chemische Synthese (DE-588)4133806-6 gnd |
| topic_facet | Microarray Polysaccharide Chemische Synthese Hochschulschrift |
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