In vivo functional mapping of single synapses in the vibrissal cortex of the mouse:
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| Format: | Thesis Book |
| Language: | English |
| Published: |
2012
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| Subjects: | |
| Online Access: | Volltext https://nbn-resolving.org/urn:nbn:de:bvb:91-diss-20121026-1113624-1-1 Inhaltsverzeichnis |
| Physical Description: | 106 Bl. Ill., graph. Darst. |
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| 245 | 1 | 0 | |a In vivo functional mapping of single synapses in the vibrissal cortex of the mouse |c Zsuzsanna Varga |
| 264 | 1 | |c 2012 | |
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| 337 | |b n |2 rdamedia | ||
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| 502 | |a München, Techn. Univ., Diss., 2012 | ||
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| 912 | |a ebook | ||
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Record in the Search Index
| _version_ | 1804151551627886592 |
|---|---|
| adam_text | TABLE OF CONTENTS
1. INTRODUCTION 7
1.1. THE
ANATOMY OF THE MOUSE WHISKER SYSTEM
9
1.1.1. PERIPHERAL SENSORY ORGANS: VIBRISSAE 9
1.1.2. SENSORY PATHWAYS CONNECTING VIBRISSAE TO 51 10
1.1.3. THE MOUSE WSL 11
1.1.3.1. BARRELS 11
1.1.3.2. CONNECTIONS OF MOUSE WSL 12
1.1.3.3. MICROCIRCUITRY OF MOUSE SI 14
1.1.3.4. MORPHOLOGY OF L2 AND L4 EXCITATORY NEURONS IN MOUSE SI 15
1.2. THE
FUNCTION OF MOUSE SI 17
1.2.1. RECEPTIVE FIELD PROPERTIES OFL2 AND L4 NEURONS IN MOUSE WSL 17
1.2.2. TOUCH REPRESENTATION IN THE MOUSE SI 18
1.3. METHODS FOR STUDYING SYNAPTIC
CONNECTIONS IN VITRO AND
IN VIVO 22
1.3.1. ANATOMICAL RECONSTRUCTIONS 22
1.3.2. ELECTROPHYSIOLOGICAL PAIR RECORDINGS 24
1.3.3. PHOTOSTIMULATION 24
1.3.3.1. OPTOGENETICS 24
1.3.3.2. GLUTAMATE UNCAGING 25
1.3.4. TWO-PHOTON CALCIUM IMAGING 25
1.4. DENDRITES
AND SPINES
27
2. AIMS 30
3. MATERIALS AND METHODS 31
3.1. ANIMALS
AND SURGICAL PROCEDURES
31
3.1.1.12 EXPERIMENTS 31
3.1.2. L4 EXPERIMENTS 32
3.2. WHISKER STIMULATION PROTOCOL 32
3.2.1. L2 EXPERIMENTS 32
3.2.2. L4 EXPERIMENTS 33
3.3. INTRINSIC
SIGNAL OPTICAL IMAGING 33
3.3.1.12 EXPERIMENTS 33
3.3.2. L4 EXPERIMENTS 34
3.4. FILLING THE NEURONS WITH CALCIUM-SENSITIVE DYE 34
3.4.1.12 EXPERIMENTS - TWO-PHOTON TARGETED WHOLE-CELL CONFIGURATION 34
3.4.2. L4 EXPERIMENTS - TWO-PHOTON TARGETED SINGLE CELL
ELECTROPORATION 34
HTTP://D-NB.INFO/1045000728
3.5. ELECTROPHYSIOLOGICAL RECORDINGS 35
3.5.1.12 EXPERIMENTS - WHOLE-CELL RECORDINGS 35
3.5.2. L4 EXPERIMENTS - LOOSE CELL-ATTACHED RECORDINGS 35
3.6.
SYNCHRONIZATION
OF ELECTRICAL RECORDINGS
WITH CALCIUM IMAGING AND STIMULATION
TIMING 36
3.6.1.12 EXPERIMENTS - PRE-SET MODE 36
3.6.2. L4 EXPERIMENTS - ARBITRARY EVENT MODE 36
3.7. TWO-PHOTON
CALCIUM IMAGING 36
3.7.1.12 EXPERIMENTS 36
3.7.2. L4 EXPERIMENTS 38
3.8. DRUG APPLICATION 38
3.8.1. L4 EXPERIMENTS 38
3.9. POST-HOC
PROCEDURES 38
3.9.1. L2 EXPERIMENTS 38
3.10. DATA ANALYSIS 39
3.10.1.12 EXPERIMENTS 39
3.10.1.1. RECONSTRUCTION OF DENDRITIC TREES 39
3.10.1.2. CALCULATION OF WHISKER-EVOKED SUBTHRESHOLD MEMBRANE POTENTIAL
DEPOLARIZATION 39
3.10.1.3. SPATIAL-TEMPORAL FILTERING OF CALCIUM IMAGING DATA AND
TRANSFORMATION INTO AF/F IMAGE SEQUENCES 39
3.10.1.4. CALCULATION OF GLOBAL DENDRITIC CALCIUM SIGNALS 40
3.10.1.5. DETECTION AND CATEGORIZATION OF DENDRITIC HOTSPOT SITES 40
3.10.1.6. CALCULATION OF CALCIUM SIGNALS OF INDIVIDUAL SPINES 41
3.10.2. L4 EXPERIMENTS 41
3.10.2.1. RECONSTRUCTION OF DENDRITIC TREES 41
3.10.2.2. AUTOMATIC RECOGNITION OF STIMULATION EVOKED RESPONSES 41
3.10.2.3. CALCULATION OF RESPONSE ONSET TIME 41
3.10.2.4. CALCULATION OF SUBTHRESHOLD RESPONSE PROBABILITIES 42
3.10.2.5. CLASSIFICATION OF SHORT LATENCY SPINES 42
4. RESULTS
43
4.1. L2 NEURONS
43
4.1.1. IMAGING DENDRITES OFL2 NEURONS IN THE MOUSE WSL 43
4.1.2. DISTANCE DEPENDENCE OF ELECTRICAL AND GLOBAL CALCIUM SIGNALS 46
4.1.3. DISTRIBUTION OF INPUTS TO L2 NEURONS 48
4.1.4. DISTANCE DEPENDENCE OF THE NUMBER OF INPUTS TO 12 NEURONS 52
4.1.5. SPINE IMAGING REVEALS SHARED SPINES 53
4.1.6. SUMMARY OF THE RESULTS OF L2
EXPERIMENTS 57
5
4.2. L4 NEURONS
59
4.2.1. TWO-PHOTON CALCIUM IMAGING OF SPINES IN L4
NEURONS IN THE MOUSE WSL 59
4.2.2. CHARACTERIZATION OF WHISKER STIMULATION EVOKED INPUTS TO L4 61
4.2.3. SPATIAL DISTRIBUTION OF SHORT LATENCY SPINES 68
4.2.4. SUMMARY OF THE RESULTS OF L4 EXPERIMENTS 71
5. DISCUSSION
72
5.1.
TECHNICAL ADVANCES AND
ASPECTS
73
5.1.1. COMMON COORDINATE SYSTEM OF INTRINSIC SIGNAL OPTICAL IMAGING AND
TWO-
PHOTON CALCIUM IMAGING 73
5.1.2. DYE FILLING APPROACHES 74
5.1.3. WHISKER STIMULATION PROTOCOLS 75
5.1.4. MORPHOLOGY RECONSTRUCTIONS FROM Z-STACKS 75
5.1.5. SPATIAL RESOLUTION OF HOTSPOTS IN DENDRITIC IMAGING 76
5.1.6. TEMPORAL RESOLUTION TO IDENTIFY EARLY RESPONSES 76
5.2.
SIMILAR PROPERTIES OF INPUTS REACHING
L2 AND L4 NEURONS
77
5.2.1. CALCIUM TRANSIENTS LOCALIZED TO SPINES 77
5.2.2. DISTRIBUTED INPUTS 77
5.2.3. TEMPORAL VARIABILITY OF INPUT ACTIVATION 78
5.3.
SPECIFIC PROPERTIES OF INPUTS REACHING
L2 NEURONS
79
5.3.1. SHARED SPINES 79
5.3.2. DISTANCE DEPENDENCE OF SW-SPECIFIC
AND SHARED SPINE 80
5.4. SPECIFIC
PROPERTIES OF INPUTS
REACHING L4 NEURONS 81
5.4.1. SHORT LATENCY INPUTS 81
5.4.2. SPATIAL DISTRIBUTION OF SHORT LATENCY INPUTS TO SPINY STELLATES
AND STAR
PYRAMIDS 81
5.5. MODEL CIRCUITRY 82
6. SUMMARY 84
7. ABBREVIATIONS 85
8. REFERENCES 86
9. ACKNOWLEDGEMENTS 105
10. PUBLICATIONS
CONTAINING
THESE RESULTS 106
6
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| physical | 106 Bl. Ill., graph. Darst. |
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| spelling | Varga, Zsuzsanna Verfasser aut In vivo functional mapping of single synapses in the vibrissal cortex of the mouse Zsuzsanna Varga 2012 106 Bl. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier München, Techn. Univ., Diss., 2012 Mit einem Abstakt in dt. Sprache (DE-588)4113937-9 Hochschulschrift gnd-content Erscheint auch als Online-Ausgabe urn:nbn:de:bvb:91-diss-20121026-1113624-1-1 http://mediatum.ub.tum.de/node?id=1113624 Verlag kostenfrei Volltext https://nbn-resolving.org/urn:nbn:de:bvb:91-diss-20121026-1113624-1-1 Resolving-System DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=026882468&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Varga, Zsuzsanna In vivo functional mapping of single synapses in the vibrissal cortex of the mouse |
| subject_GND | (DE-588)4113937-9 |
| title | In vivo functional mapping of single synapses in the vibrissal cortex of the mouse |
| title_auth | In vivo functional mapping of single synapses in the vibrissal cortex of the mouse |
| title_exact_search | In vivo functional mapping of single synapses in the vibrissal cortex of the mouse |
| title_full | In vivo functional mapping of single synapses in the vibrissal cortex of the mouse Zsuzsanna Varga |
| title_fullStr | In vivo functional mapping of single synapses in the vibrissal cortex of the mouse Zsuzsanna Varga |
| title_full_unstemmed | In vivo functional mapping of single synapses in the vibrissal cortex of the mouse Zsuzsanna Varga |
| title_short | In vivo functional mapping of single synapses in the vibrissal cortex of the mouse |
| title_sort | in vivo functional mapping of single synapses in the vibrissal cortex of the mouse |
| topic_facet | Hochschulschrift |
| url | http://mediatum.ub.tum.de/node?id=1113624 https://nbn-resolving.org/urn:nbn:de:bvb:91-diss-20121026-1113624-1-1 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=026882468&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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