Annexins in epithelial differentiation:
Saved in:
| Main Author: | |
|---|---|
| Format: | Thesis Book |
| Language: | English |
| Published: |
2012
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| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | 156 S. Ill., graph. Darst. |
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MARC
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| 245 | 1 | 0 | |a Annexins in epithelial differentiation |c vorgelegt von: Nina Quiskamp |
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| 650 | 0 | 7 | |a Epithel |0 (DE-588)4130937-6 |2 gnd |9 rswk-swf |
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Record in the Search Index
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|---|---|
| adam_text | TABLE OF CONTENTS
1 INTRODUCTION 9
1.1 ANNEXINS IN EPITHELIAL-MESENCHYMAL CELL TRANSFORMATION 9
1.2 STRUCTURAL AND BIOCHEMICAL CHARACTERISTICS OF THE ANNEXIN FAMILY 13
1.2.1 THE ANNEXIN CORE-DOMAIN 13
1.2.2 THE N-TERMINAL INTERACTION DOMAIN 15
1.3 ANNEXIN A2 16
1.3.1 CHARACTERISTICS OF ANNEXIN A2 16
1.3.2 POST-TRANSLATIONAL MODIFICATIONS OF ANNEXIN A2 19
1.3.2.1 SERINE PHOSPHORYLATION OF ANNEXIN A2 19
1.3.2.2 TYROSINE PHOSPHORYLATION OF ANNEXIN A2 20
1.4 ANNEXIN A10 22
1.4.1 THE STRUCTURAL CHARACTERISTICS OF ANXALO 22
1.4.2 ANXALO EXPRESSION IN HEALTH AND DISEASE 23
1.4.3 BIOCHEMICAL CHARACTERISTICS AND SUBCELLULAR DISTRIBUTION OF ANXALO
24
2. MATERIALS 25
2.1. CHEMICALS & REAGENTS 25
2.2. EUKARYOTIC AND BACTERIAL EXPRESSION VECTORS 26
2.3. CELL LINES 28
2.4. PRIMARY ANTIBODIES 29
2.5. SECONDARY ANTIBODIES 30
2.6. GENERAL MATERIALS & EQUIPMENT 31
3. METHODS 32
3.1. CELL BIOLOGY METHODS 32
3.1.1. CULTIVATION OF EUKARYOTIC CELLS 32
3.1.2. PASSAGING OF CELLS 32
3.1.3. CRYO-STOCKS 32
3.1.4. TRANSFECTION OF EUKARYOTIC CELLS 33
5
HTTP://D-NB.INFO/1044197919
3.1.5. FLOW CYTOMETRIC ANALYSIS OF CELL LINES 35
3.1.6. CONFOCAL IMMUNOFLUORESCENCE IMAGING 35
3.2. BIOCHEMICAL METHODS 38
3.2.1. BACTERIAL EXPRESSION AND PURIFICATION ANNEXIN ALO-SHORT 38
3.2.2. CELL LYSATES 39
3.2.3. CELL FRACTIONATIONS 40
3.2.4. BRADFORD PROTEIN ASSAY 43
3.2.5. IMMUNOPRECIPITATION 43
3.2.6. DISCONTINUOUS SDS-PAGE 44
3.2.7. WESTERN BLOT ANALYSIS 45
3.2.8. IMMUNODETECTION OF PROTEINS 45
3.2.9. STRIPPING OF BLOT MEMBRANES 46
3.3. PROJECT RELEVANT ASSAYS 47
3.3.1. 3D-MDCK II CYST ASSAYS 47
3.3.1.1. COLLAGEN SYSTEM 47
3.3.1.2. MATRIGEL-SYSTEM 48
3.3.2. PHOSPHORYLATION ASSAYS 49
3.3.3. XM-CLUSTER ASSAY 49
3.3.4. APOPTOSIS ASSAY 49
3.3.5. PROTEIN EXPRESSION IN POLARIZING MDCK II CELLS 50
RESULTS 51
4.1 CHARACTERIZATION OF ANXA2 MUTANTS 51
4.1.1 CHARACTERIZATION OF A PTYR23-ANXA2 SPECIFIC ANTIBODY 51
4.1.2 IN VIVO CHARACTERIZATION OF ANXA2-MUTANTS 56
4.1.3 ANALYSIS OF ANXA2 DISTRIBUTION IN MDCK II CYSTS 72
4.2 ANXALO IS A COMPONENT OF NUCLEAR SUBCOMPARTMENTS AND ACTS IN A TUMOR
SUPPRESSIVE
MANNER 77
4.2.1 BIOCHEMICAL AND FUNCTIONAL CHARACTERIZATION OF ANXALO 77
6
4.2.2 BIOCHEMICAL CHARACTERIZATIONS OF ANXALO 84
4.2.3 ANALYSIS OF SUBCELLULAR ANXALO AND ANXALO-SHORT DISTRIBUTION 88
4.2.4 ANXALO CO-LOCALIZATION WITH NUCLEAR SUB-COMPARTMENTS 96
5 DISCUSSION ILL
5.1 FUNCTIONAL REGULATION OF ANXA2 ILL
5.2 ANNEXIN A10 IS A NOVEL COMPONENT OF NUCLEAR SUBCOMPARTMENTS AND ACTS
IN A TUMOR
SUPPRESSIVE MANNER 126
ACKNOWLEDGEMENTS / DANKSAGUNG 154
7
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| author | Quiskamp, Nina 1980- |
| author_GND | (DE-588)1044197854 |
| author_facet | Quiskamp, Nina 1980- |
| author_role | aut |
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| bvnumber | BV041418441 |
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| dewey-full | 571.553835 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 571 - Physiology & related subjects |
| dewey-raw | 571.553835 |
| dewey-search | 571.553835 |
| dewey-sort | 3571.553835 |
| dewey-tens | 570 - Biology |
| discipline | Biologie |
| format | Thesis Book |
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| indexdate | 2024-07-10T00:56:20Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-026865570 |
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| physical | 156 S. Ill., graph. Darst. |
| publishDate | 2012 |
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| spelling | Quiskamp, Nina 1980- Verfasser (DE-588)1044197854 aut Annexins in epithelial differentiation vorgelegt von: Nina Quiskamp 2012 156 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Münster (Westfalen), Univ., Diss., 2012 Zelldifferenzierung (DE-588)4067536-1 gnd rswk-swf Annexine (DE-588)4253644-3 gnd rswk-swf Epithel (DE-588)4130937-6 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Annexine (DE-588)4253644-3 s Epithel (DE-588)4130937-6 s Zelldifferenzierung (DE-588)4067536-1 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=026865570&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Quiskamp, Nina 1980- Annexins in epithelial differentiation Zelldifferenzierung (DE-588)4067536-1 gnd Annexine (DE-588)4253644-3 gnd Epithel (DE-588)4130937-6 gnd |
| subject_GND | (DE-588)4067536-1 (DE-588)4253644-3 (DE-588)4130937-6 (DE-588)4113937-9 |
| title | Annexins in epithelial differentiation |
| title_auth | Annexins in epithelial differentiation |
| title_exact_search | Annexins in epithelial differentiation |
| title_full | Annexins in epithelial differentiation vorgelegt von: Nina Quiskamp |
| title_fullStr | Annexins in epithelial differentiation vorgelegt von: Nina Quiskamp |
| title_full_unstemmed | Annexins in epithelial differentiation vorgelegt von: Nina Quiskamp |
| title_short | Annexins in epithelial differentiation |
| title_sort | annexins in epithelial differentiation |
| topic | Zelldifferenzierung (DE-588)4067536-1 gnd Annexine (DE-588)4253644-3 gnd Epithel (DE-588)4130937-6 gnd |
| topic_facet | Zelldifferenzierung Annexine Epithel Hochschulschrift |
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