Role of innate immune receptors in chemical-induced contact hypersensitivity: identification and modulation of novel inflammatory pathways in contact hypersensitivity
Gespeichert in:
| 1. Verfasser: | |
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| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
2013
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| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Enth. 2 Sonderabdr. aus: The journal of experimental medicine ; Vol. 207. 2010 und 2013 (eingereicht) |
| Beschreibung: | 140 Bl. Ill., graph. Darst. 30 cm |
Internformat
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Datensatz im Suchindex
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| adam_text | IMAGE 1
TABLE OF CONTENT
1. SUMMARY 8
2. ZUSAMMENFASSUNG 9
3. INTRODUCTION 11
3.1. ALLERGIC CONTACT DERMATITIS 11
3.1.1. CONTACT HYPERSENSITIVITY 13
3.1.2. SKIN 13
3.2. THE IMMUNE SYSTEM 14
3.2.1. INNATE IMMUNITY 15
3.2.2. DENDRITIC CELLS 16
3.2.3. NEUTROPHILS 18
3.2.4. MAST CELLS 21
3.2.5. KERATINOCYTES 21
3.3. TRIGGERING INNATE INFLAMMATORY PATHWAYS 22
3.3.1. TOLL-LIKE RECEPTORS 22
3.3.2. INTERLEUKIN-L|3 AND THE INFLAMMASOME 23
3.3.3. THE LINK BETWEEN EXTRACELLULAR ATP AND INFLAMMASOME ACTIVATION 26
3.3.4. REACTIVE OXYGEN SPECIES 27
3.3.5. DAMAGE ASSOCIATED MOLECULAR PATTERNS IN STERILE INFLAMMATION 28
3.4. ADAPTIVE IMMUNITY 29
3.4.1. T CELLS 29
4. AIM OF THE WORK 31
4.1. IDENTIFICATION OF THE MOLECULAR PATHWAY OF INFLAMMASOME ACTIVATION
IN CHS 31 4.2. INVESTIGATION OF THE ROLE OF NEUTROPHILS IN CHS 31
5. MANUSCRIPTS 33
5.1. LACK OF THE PURINERGIC RECEPTOR P2X7 RESULTS IN RESISTANCE TO
CONTACT HYPERSENSITIVITY 33
5.2. NEUTROPHILS ARE CRUCIAL INNATE EFFECTOR CELLS IN CONTACT
HYPERSENSITIVITY 47 6. UNPUBLISHED RESULTS 73
6.1. INVESTIGATION OF THE ROLE OF TREG IN THE PHENOTYPE OF P2X7
DEFICIENT MICE 73 6.2. IN VITRO ANALYSIS OF CONTACT ALLERGEN INDUCED ROS
PRODUCTION BY NEUTROPHILS. 75 6.3. IS THE PHENOTYPE OF NEUTROPHIL
DEPLETED MICE CAUSED BY LACK OF IL-IP? 78
6.4. MOLECULAR MECHANISM OF NEUTROPHIL ACTIVATION IN CHS 79
7. DISCUSSION ; 84
7.1. TISSUE MICROENVIRONMENT AND INFLAMMATION 84
7.2. INFLAMMASOME ACTIVATION IN CHS 84
7.3. THE PATHWAY OF INFLAMMASOME ACTIVATION- OPPORTUNITIES FOR
THERAPEUTIC INTERVENTION 86
7.4. UNDERSTANDING THE ROLE OF NEUTROPHILS IN CHS 87
7.5. THE ROLE OF NEUTROPHILS IN THE GENERATION OF CONTACT ALLERGEN
INDUCED SKIN INFLAMMATION 88
7.6. THE CONTRIBUTION OF NEUTROPHILS TO DENDRITIC CELL ACTIVATION AND T
CELL PRIMING 90 7.7. UNDERSTANDING THE ROLE OF NEUTROPHILS IN THE
ELICITATION PHASE OF CHS 91
7.1. CONCLUSION 92
7.2. OUTLOOK 94
8. MATERIAL AND METHODS 95
8.1. MATERIAL 95
6
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IMAGE 2
8.1.1. ANTIBODIES 95
8.1.2. CHEMICALS 102
8.1.3. MICE 103
8.2. BUFFERS AND MEDIA 104
8.2.1. RP-10 MEDIUM 104
8.2.2. TRYPAN BLUE FOR CELL COUNTING 104
8.2.3. FACS BUFFER 104
8.2.4. ERYTHROCYTE LYSIS BUFFER FOR BMDC GENERATION 104
8.2.5. BUFFER FOR AGAROSE GEL ELECTROPHORESIS 105
8.3. IN VITRO METHODS 106
8.3.1. GENERATION OF BMDCS 106
8.3.2. CULTIVATION OF NMPR14 CELLS 107
8.3.3. PURIFICATION OF IGG 108
8.3.4. IN VITRO SUPEROXIDE RELEASE ASSAY 110
8.4. IN VIVO AND EX VIVO METHODS 111
8.4.1. TNCB INDUCED CHS MODEL 111
8.4.2. OXAZOLONE INDUCED CHS MODEL 112
8.4.3. CROTON OIL INDUCED ICD 113
8.4.4. DEPLETION OF NEUTROPHILS 114
8.4.5. FITC LYMPH NODE MIGRATION ASSAY 115
8.4.6. IN VIVO ROS MEASUREMENT ASSAY 115
8.4.7. IN VIVO ATP RELEASE ASSAY 115
8.4.8. ADOPTIVE CHS MODEL 116
8.4.9. SENSITIZATION WITH HAPTEN MODIFIED BMDCS 117
8.4.10. DIGESTION OF EAR SKIN BY BLENDZYME 118
8.4.11. EX VIVO GELATINASE ASSAY 120
8.4.12. RE-STIMULATION OF LYMPH NODE CELLS 123
8.4.13. PURIFICATION OF NEUTROPHILS 125
8.4.14. CELL CULTURE 126
8.4.15. FACS-ANALYSIS 126
8.4.16. ELISAS 127
8.4.17. EAR SHEET CULTURES AND MEASUREMENT OF IL-IP PRODUCTION 127
9. ABBREVIATIONS 128
10. ACKNOWLEDGEMENT 130
11. REFERENCES 131
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| spelling | Weber, Felix C. Verfasser aut Role of innate immune receptors in chemical-induced contact hypersensitivity identification and modulation of novel inflammatory pathways in contact hypersensitivity vorgelegt von Felix C. Weber 2013 140 Bl. Ill., graph. Darst. 30 cm txt rdacontent n rdamedia nc rdacarrier Enth. 2 Sonderabdr. aus: The journal of experimental medicine ; Vol. 207. 2010 und 2013 (eingereicht) Freiburg (Breisgau), Univ., Diss., 2013 (DE-588)4113937-9 Hochschulschrift gnd-content DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=026234254&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Weber, Felix C. Role of innate immune receptors in chemical-induced contact hypersensitivity identification and modulation of novel inflammatory pathways in contact hypersensitivity |
| subject_GND | (DE-588)4113937-9 |
| title | Role of innate immune receptors in chemical-induced contact hypersensitivity identification and modulation of novel inflammatory pathways in contact hypersensitivity |
| title_auth | Role of innate immune receptors in chemical-induced contact hypersensitivity identification and modulation of novel inflammatory pathways in contact hypersensitivity |
| title_exact_search | Role of innate immune receptors in chemical-induced contact hypersensitivity identification and modulation of novel inflammatory pathways in contact hypersensitivity |
| title_full | Role of innate immune receptors in chemical-induced contact hypersensitivity identification and modulation of novel inflammatory pathways in contact hypersensitivity vorgelegt von Felix C. Weber |
| title_fullStr | Role of innate immune receptors in chemical-induced contact hypersensitivity identification and modulation of novel inflammatory pathways in contact hypersensitivity vorgelegt von Felix C. Weber |
| title_full_unstemmed | Role of innate immune receptors in chemical-induced contact hypersensitivity identification and modulation of novel inflammatory pathways in contact hypersensitivity vorgelegt von Felix C. Weber |
| title_short | Role of innate immune receptors in chemical-induced contact hypersensitivity |
| title_sort | role of innate immune receptors in chemical induced contact hypersensitivity identification and modulation of novel inflammatory pathways in contact hypersensitivity |
| title_sub | identification and modulation of novel inflammatory pathways in contact hypersensitivity |
| topic_facet | Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=026234254&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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