Generation of an infectious clone of Duck Enteritis Virus (DEV): a basis for pathogenesis studies and vectored vaccine development
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| Main Author: | |
|---|---|
| Format: | Thesis Book |
| Language: | English |
| Published: |
Berlin
Mensch und Buch Verl.
2011
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| Subjects: | |
| Online Access: | Volltext Inhaltsverzeichnis |
| Item Description: | Nebentitel: Generierung eines infektiösen Duck Enteritis Virus (DEV) Klons |
| Physical Description: | VIII, 95 S. Ill., graph. Darst. |
| ISBN: | 9783863870232 |
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MARC
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| 245 | 1 | 0 | |a Generation of an infectious clone of Duck Enteritis Virus (DEV) |b a basis for pathogenesis studies and vectored vaccine development |c vorgelegt von Jichun Wang |
| 246 | 1 | 3 | |a Generierung eines infektiösen Duck-Enteritis-Virus-(DEV)-Klons |
| 264 | 1 | |a Berlin |b Mensch und Buch Verl. |c 2011 | |
| 300 | |a VIII, 95 S. |b Ill., graph. Darst. | ||
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| 500 | |a Nebentitel: Generierung eines infektiösen Duck Enteritis Virus (DEV) Klons | ||
| 502 | |a Berlin, Freie Univ., Diss., 2011 | ||
| 650 | 7 | |a duck enteritis virus |2 cabt | |
| 650 | 7 | |a duck plague virus |2 cabt | |
| 650 | 7 | |a anatid herpesvirus 1 |2 cabt | |
| 650 | 7 | |a bacterial artificial chromosomes |2 cabt | |
| 650 | 7 | |a glycoproteins |2 cabt | |
| 650 | 7 | |a vectors |2 cabt | |
| 650 | 7 | |a avian influenza virus |2 cabt | |
| 650 | 7 | |a hemagglutinins |2 cabt | |
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| 650 | 7 | |a amino acid |2 cabt | |
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| 650 | 7 | |a nucleotide |2 cabt | |
| 650 | 7 | |a virulence |2 cabt | |
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Record in the Search Index
| _version_ | 1804150707312394240 |
|---|---|
| adam_text | TABLE OF CONTENTS
TABLE OF CONTENTS
TABLE OF CONTENTS I
LIST OF FIGURES IV
LIST OF TABLES V
ABBREVIATIONS VI
ZUSAMMENFASSUNG 1
SUMMARY 3
CHAPTER 1. INTRODUCTION 5
1.1. TAXANOMY AND MORPHOLOGY OF DEV 5
1.2. REPLICATION OF DEV 6
1.3. PATHOLOGY OF DUCK PLAGUE 7
1.4. EPIDEMIOLOGY OF DUCK PLAGUE 8
1.5. TRANSMISSION OF DEV 11
1.6. FUNCTIONS OF DEV PROTEINS 12
1.7. DIAGNOSIS OF DEV 13
1.7.1. SEROLOGICALASSAYS 14
1.7.2. VIRUS DETECTION 14
1.8. VACCINES AGAINST DUCK PLAGUE 15
1.9. DUCKS AND THEIR ROLE IN AVIAN INFLUENZA EPIDEMIOLOGY 17
REFERENCES 18
CHAPTER 2. GENERATION OF AN INFECTIOUS CLONE OF DUCK ENTERITIS VIRUS AND
GENERATION OF A
VECTORED DEV EXPRESSING HEMAGGLUTININ OF H5N1 AVIAN INFLUENZA VIRUS 25
2.1. SUMMARY 25
2.2. INTRODUCTION 25
2.3. MATERIALSAND METHODS 27
2.3.1. PLASMIDS 27
2.3.2. VIRUSES, CELLS AND TRANSFECTION 28
2.3.3. MULTI-STEP GROWTH KINETICS AND PLAQUE SIZES DETERMINATIONS 29
2.3.4. BACTERIAL MANIPULATIONS 29
2.3.5. GENERATION OF A DEV BAC CLONE 30
BIBLIOGRAFISCHE INFORMATIONEN
HTTP://D-NB.INFO/1015695361
DIGITALISIERT DURCH
TABLE OF CONTENTS
2.3.6. DIAGNOSTIC PCR AND SEQUENCING 31
2.3.7. INDIRECT IMMUNOFLUORESCENCE (IIF) AND WESTERN BLOTTING 31
2.4. RESULTS AND DISCUSSION 32
2.4.1. GENERATION OF MUTANT DEV STRAIN 2085 HARBORING MINI-F PLASMID
SEQUENCES 32
2.4.2. GENERATION OF INFECTIOUS DEV BAC CLONE P2085 33
2.4.3. GENERATION OF P2085_H5 35
2.4.4. RECONSTITUTION OF RECOMBINANT DEV FROM CLONED DNA AND GENERATION
OF MUTANT
VIRUSES 35
2.4.5. ROBUST EXPRESSION OF AIV HA BY RECOMBINANT DEV V2085_H5 37
2.4.6. GROWTH PROPERTIES OF RECOMBINANT DEV 37
2.5. CONCLUSIONS 40
REFERENCES 41
CHAPTER 3. COMPLETE GENOME SEQUENCE OF VIRULENT DUCK ENTERITIS VIRUS
STRAIN 2085 AND
COMPARISON WITH GENOME SEQUENCES OF VIRULENT AND ATTENUATED DEV STRAINS
49
3.1. SUMMARY 49
3.2. INTRODUCTION 49
3.3. MATERIALSAND METHODS 51
3.3.7. VIRUS, CELLS AND BAC 51
3.3.2. DNA ISOLATION AND GENOME SEQUENCING 51
3.3.3 DNA SEQUENCE ANALYSIS 51
3.4. RESULTS AND DISCUSSION 52
3.4.1. GENOMIC ORGANIZATION OF DEV STRAIN 2085 52
3.4.2. COMPARISON OF ORFS BETWEEN DEV STRAINS 2085, VAC, CLONE-03 AND
CHV. 53
3.4.2.1. VARIATIONS IN LORF11 54
3.4.2.2. VARIATIONS IN UL2 55
3.4.2.3. VARIATIONS IN UL9 56
3.4.2.4. VARIATIONS IN LORF3 56
3.4.2.5. VARIATIONS IN OTHER ORFS PRESENT IN THE UNIQUE-LONG GENOME
SEGMENT 56
3.4.2.6. VARIATIONS IN OTHER ORFS PRESENT IN THE UNIQUE-SHORT GENOME
SEGMENT 61
TABLE OF CONTENTS
3.5. CONCLUDING REMARKS 64
REFERENCES 64
SUPPLEMENTARY TABLE 1 70
SUPPLEMENTARY TABLE 2 73
CHAPTER 4. DISCUSSION 75
4.1. CONSTRUCTION OF A DEV BAC 75
4.2. APPLICATIONS OF THE ENGINEERED DEV BAC 76
4.3. GLYCOPROTEINS OF DEV 78
4.4. A DEV-VECTORED H5 VACCINE CANDIDATE 80
4.5. COMPARISON OF DEV GENOMES 81
REFERENCES 83
PUBLICATIONS 93
ACKNOWLEDGEMENTS 94
|
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| author | Wang, Jichun |
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| author_sort | Wang, Jichun |
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| dewey-ones | 636 - Animal husbandry |
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| dewey-sort | 3636.5089691 |
| dewey-tens | 630 - Agriculture and related technologies |
| discipline | Agrar-/Forst-/Ernährungs-/Haushaltswissenschaft / Gartenbau |
| format | Thesis Book |
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| indexdate | 2024-07-10T00:43:12Z |
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| isbn | 9783863870232 |
| language | English |
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| spelling | Wang, Jichun Verfasser (DE-588)1015943373 aut Generation of an infectious clone of Duck Enteritis Virus (DEV) a basis for pathogenesis studies and vectored vaccine development vorgelegt von Jichun Wang Generierung eines infektiösen Duck-Enteritis-Virus-(DEV)-Klons Berlin Mensch und Buch Verl. 2011 VIII, 95 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Nebentitel: Generierung eines infektiösen Duck Enteritis Virus (DEV) Klons Berlin, Freie Univ., Diss., 2011 duck enteritis virus cabt duck plague virus cabt anatid herpesvirus 1 cabt bacterial artificial chromosomes cabt glycoproteins cabt vectors cabt avian influenza virus cabt hemagglutinins cabt genomes cabt amino acid cabt sequences cabt nucleotide cabt virulence cabt vaccines cabt (DE-588)4113937-9 Hochschulschrift gnd-content Erscheint auch als Online-Ausgabe urn:nbn:de:kobv:188-fudissthesis000000025137-0 http://www.diss.fu-berlin.de/diss/receive/FUDISS_thesis_000000025137 kostenfrei Volltext DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=026226105&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Wang, Jichun Generation of an infectious clone of Duck Enteritis Virus (DEV) a basis for pathogenesis studies and vectored vaccine development duck enteritis virus cabt duck plague virus cabt anatid herpesvirus 1 cabt bacterial artificial chromosomes cabt glycoproteins cabt vectors cabt avian influenza virus cabt hemagglutinins cabt genomes cabt amino acid cabt sequences cabt nucleotide cabt virulence cabt vaccines cabt |
| subject_GND | (DE-588)4113937-9 |
| title | Generation of an infectious clone of Duck Enteritis Virus (DEV) a basis for pathogenesis studies and vectored vaccine development |
| title_alt | Generierung eines infektiösen Duck-Enteritis-Virus-(DEV)-Klons |
| title_auth | Generation of an infectious clone of Duck Enteritis Virus (DEV) a basis for pathogenesis studies and vectored vaccine development |
| title_exact_search | Generation of an infectious clone of Duck Enteritis Virus (DEV) a basis for pathogenesis studies and vectored vaccine development |
| title_full | Generation of an infectious clone of Duck Enteritis Virus (DEV) a basis for pathogenesis studies and vectored vaccine development vorgelegt von Jichun Wang |
| title_fullStr | Generation of an infectious clone of Duck Enteritis Virus (DEV) a basis for pathogenesis studies and vectored vaccine development vorgelegt von Jichun Wang |
| title_full_unstemmed | Generation of an infectious clone of Duck Enteritis Virus (DEV) a basis for pathogenesis studies and vectored vaccine development vorgelegt von Jichun Wang |
| title_short | Generation of an infectious clone of Duck Enteritis Virus (DEV) |
| title_sort | generation of an infectious clone of duck enteritis virus dev a basis for pathogenesis studies and vectored vaccine development |
| title_sub | a basis for pathogenesis studies and vectored vaccine development |
| topic | duck enteritis virus cabt duck plague virus cabt anatid herpesvirus 1 cabt bacterial artificial chromosomes cabt glycoproteins cabt vectors cabt avian influenza virus cabt hemagglutinins cabt genomes cabt amino acid cabt sequences cabt nucleotide cabt virulence cabt vaccines cabt |
| topic_facet | duck enteritis virus duck plague virus anatid herpesvirus 1 bacterial artificial chromosomes glycoproteins vectors avian influenza virus hemagglutinins genomes amino acid sequences nucleotide virulence vaccines Hochschulschrift |
| url | http://www.diss.fu-berlin.de/diss/receive/FUDISS_thesis_000000025137 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=026226105&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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