Stalk structures in lipid bilayer fusion studied by x-ray diffraction:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
Göttingen
Univ.-Verl. Göttingen
2012
|
| Schriftenreihe: | Göttingen series in x-ray physics
6 |
| Schlagworte: | |
| Online-Zugang: | Beschreibung für Marketing Inhaltsverzeichnis |
| Beschreibung: | Zusätzliches Online-Angebot unter www.sub.uni-goettingen.de |
| Beschreibung: | II, 182 S. Ill., graph. Darst. 24 cm |
| ISBN: | 9783863950439 |
Internformat
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Datensatz im Suchindex
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|---|---|
| adam_text | IMAGE 1
CONTENTS
INTRODUCTION 1
1 ELEMENTS O F LIPID BILAYER FUSION 5
1.1 LIPIDS 5
1.2 PHOSPHOLIPID MESOPHASES 7
1.3 BILAYER FUSION PATHWAYS 10
1.4 INTERACTION POTENTIALS BETWEEN LIPID BILAYERS 11
1.5 CONTINUUM THEORY A N D SIMULATIONS OF STALKS 14
2 X-RAY DIFFRACTION O N LIPID MESOPHASES 17
2.1 FUNDAMENTALS OF X-RAY DIFFRACTION 17
2.2 ORIENTED SAMPLES 20
2.3 EXPERIMENTAL TECHNIQUES 22
2.3.1 X-RAY REFLECTIVITY 22
2.3.2 GRAZING-INCIDENCE X-RAY DIFFRACTION 23
2.3.3 POWDER DIFFRACTION 25
2.4 INTENSITY CORRECTION FACTORS 26
2.5 THE PHASE PROBLEM 29
2.6 RESOLUTION 31
3 LYOTROPIC LIPID POLYMORPHISM 35
3.1 HYDRATION CONTROL B Y WATER VAPOUR 35
3.2 EXPERIMENTAL 37
3.2.1 SAMPLE PREPARATION 37
3.2.2 SAMPLE ENVIRONMENT 38
3.2.3 IN-HOUSE SMALL-ANGLE X-RAY SCATTERING SETUP 39
3.2.4 PHASE DIAGRAM DETERMINATION 4 1
3.3 RESULTS 41
3.3.1 CHARACTERISTIC DIFFRACTION PATTERNS 41
3.3.2 PHASE DIAGRAMS 42
3.3.3 INDEXING OF DIFFRACTION PATTERNS 48
3.4 CONCLUSIONS 55
4 BILAYER STRUCTURE AND INTERACTIONS 57
4.1 ELECTRON DENSITY PROFILES 57
4.2 OSMOTIC STRESS M E T H O D 58
4.3 MOTIVATION OF O U R WORK 61
4.4 EXPERIMENTAL 62
4.4.1 X-RAY REFLECTIVITY INSTRUMENT 62
4.4.2 MEASUREMENTS 63
4.4.3 DATA REDUCTION 63
4.4.4 FOURIER SYNTHESIS OF ELECTRON DENSITY PROFILES 66
4.4.5 PHASE RETRIEVAL: THE SWELLING M E T H O D 66
4.5 RESULTS A N D DISCUSSION 72
HTTP://D-NB.INFO/1020601833
IMAGE 2
II CONTENTS
4.5.1 EFFECTS A T CLOSE BILAYER SEPARATION 72
4.5.2 BILAYER STRUCTURE 72
4.5.3 HYDRATION FORCE PARAMETERS 74
4.5.4 HYDRATION BARRIER FOR M E M B R A N E FUSION 79
4.6 CONCLUSIONS 81
5 STRUCTURE DETERMINATION O F STALKS 83
5.1 DATA COLLECTION A N D REDUCTION 83
5.1.1 SYNCHROTRON GIXD EXPERIMENTS 83
5.1.2 X-RAY REFLECTIVITY EXPERIMENTS 93
5.1.3 POWDER DIFFRACTION EXPERIMENTS 94
5.1.4 COMBINATION OF DIFFERENT X-RAY GEOMETRIES 98
5.2 SOLVING T H E P H A S E PROBLEM 98
5.3 RESULTS 106
5.4 DISCUSSION 112
5.5 CONCLUSIONS 113
6 ANALYSIS O F THE 3D ELECTRON DENSITY O F STALKS 115
6.1 STRUCTURAL PARAMETERS OF THE STALK P H A S E 115
6.1.1 DEFINITIONS 115
6.1.2 RESULTS 117
6.2 ELECTRON DENSITY ISOSURFACE ANALYSIS 119
6.2.1 MOTIVATION 119
6.2.2 DIFFERENTIAL GEOMETRY OF IMPLICIT SURFACES 121
6.2.3 IMPLEMENTATION 122
6.2.4 RESULTS 124
6.3 DISCUSSION A N D COMPARISON TO C O N T I N U U M THEORY 131
6.3.1 GENERAL REMARKS 131
6.3.2 POSSIBLE LIMITATIONS OF C O N T I N U U M THEORY 131
6.3.3 COMPARISON TO C O N T I N U U M MODELS 132
6.4 CONCLUSIONS 134
7 SUMMARY 135
A APPENDIX 137
A.L TRIPLET RELATIONSHIP 137
A.2 CRYSTALLOGRAPHIC D A T A A N D SWELLING DIAGRAMS 139
A.3 GAUSS-BONNET T H E O R E M APPLIED TO STALKS 156
A . 4 MATLAB CODE: EXAMPLES 1 5 8
REFERENCES 171
LIST O F PUBLICATIONS 183
DANKSAGUNG 185
CURRICULUM VITAE 187
|
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| isbn | 9783863950439 |
| language | English |
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| physical | II, 182 S. Ill., graph. Darst. 24 cm |
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| spelling | Aeffner, Sebastian 1981- Verfasser (DE-588)102249516X aut Stalk structures in lipid bilayer fusion studied by x-ray diffraction Sebastian Aeffner Göttingen Univ.-Verl. Göttingen 2012 II, 182 S. Ill., graph. Darst. 24 cm txt rdacontent n rdamedia nc rdacarrier Göttingen series in x-ray physics 6 Zusätzliches Online-Angebot unter www.sub.uni-goettingen.de Zugl.: Göttingen, Univ., Diss., 2011 (DE-588)4113937-9 Hochschulschrift gnd-content Göttingen series in x-ray physics 6 (DE-604)BV037380493 6 X:MVB text/html http://www.univerlag.uni-goettingen.de/content/list.php?notback=1&details=isbn-978-3-86395-043-9 Beschreibung für Marketing DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=026183078&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Aeffner, Sebastian 1981- Stalk structures in lipid bilayer fusion studied by x-ray diffraction Göttingen series in x-ray physics |
| subject_GND | (DE-588)4113937-9 |
| title | Stalk structures in lipid bilayer fusion studied by x-ray diffraction |
| title_auth | Stalk structures in lipid bilayer fusion studied by x-ray diffraction |
| title_exact_search | Stalk structures in lipid bilayer fusion studied by x-ray diffraction |
| title_full | Stalk structures in lipid bilayer fusion studied by x-ray diffraction Sebastian Aeffner |
| title_fullStr | Stalk structures in lipid bilayer fusion studied by x-ray diffraction Sebastian Aeffner |
| title_full_unstemmed | Stalk structures in lipid bilayer fusion studied by x-ray diffraction Sebastian Aeffner |
| title_short | Stalk structures in lipid bilayer fusion studied by x-ray diffraction |
| title_sort | stalk structures in lipid bilayer fusion studied by x ray diffraction |
| topic_facet | Hochschulschrift |
| url | http://www.univerlag.uni-goettingen.de/content/list.php?notback=1&details=isbn-978-3-86395-043-9 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=026183078&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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