Neural development: methods and protocols
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
New York, NY [u.a.]
Humana Press
2013
|
| Schriftenreihe: | Methods in molecular biology
1018 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XIII, 347 S. Ill., graph. Darst. |
| ISBN: | 9781627034432 |
Internformat
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| 100 | 1 | |a Zhou, Renping |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a Neural development |b methods and protocols |c ed. by Renping Zhou ... |
| 264 | 1 | |a New York, NY [u.a.] |b Humana Press |c 2013 | |
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Datensatz im Suchindex
| _version_ | 1804150327219322880 |
|---|---|
| adam_text | Contents
Preface
..........................................................
v
Contributors
.......................................................... xi
Part I Neuron and Glial Cell Culture
1
Isolation and Maintenance of Cortical Neural Progenitor Cells In Vitro
..... 3
Mi-Yeon Kim, Byoung-San Moon, and Kang-TTell Choi
2
Neural Induction with a Dopaminergic Phenotype
from Human Pluripotent Stem Cells Through a Feeder-Free Floating
Aggregation Culture
............................................ 11
Asuka Morizane, Daisuke
Doi,
and
Jun
Takahashi
3
Nucleoside Analog Labeling of Neural Stem Cells and Their Progeny
....... 21
Erik Michael DeBoer and Mladen-Roko Rasin
4
Culture of Dissociated Hippocampal Neurons
......................... 39
Tun
Pen¿f,
Wen-Cheng Xiong, and Lin
Mei
5
Culturing Mouse Cerebellar Granule Neurons
........................ 49
Tharakeswari Selvakumar and Daniel L.
Kilpatrick
6
Preparation of Primary Cultured Dopaminergic Neurons
from Mouse Brain
.............................................. 61
Won-Seok Choi, Hyung-Wook Kim, and Zhengui Xia
7
Culturing Astrocytes from Postnatal Rats
............................ 71
Tangyang
Huang and Cheryl F. Dreyfus
8
Culturing Oligodendrocyte Lineage Cells from Neonatal Rats
............. 81
Clifton G. Fulmer and Cheryl F. Dreyfus
9
Isolation and Culture of Schwann Cells
.............................. 93
Yanmei Tao
Part II Gene Expression and Analysis
10 DNA Transfection:
Calcium Phosphate Method
....................... 107
Munjin Kwon and Bonnie L. Firestein
11
The Gene-Gun Approach for Transfection and Labeling
of Cells in Brain Slices
...........................................
Ill
Anna Dtmaevsky
12
Lentiviral Vector Production,
Titration,
and Transduction
of Primary Neurons
............................................ 119
Baojin Ding and Daniel L.
Kilpatrick
VII
viii Contents
13
In
Ovo
Electroporation in
Embryonic Chick
Spinal Cords............... 133
Hui
Wang
and Michael P. Matise
14 Gene Transfer in
Developing Chick
Embryos: In
Ovo
Electroporation...... 141
Emma K.
Farley
15 In Utero Electroporation in
Mice
.................................. 151
Chitnlei
Wang and Lin
Mei
16 General
Introduction
to In
Sito
Hybridization Protocol Using
Nonradioactively Labeled Probes to Detect mRNAs on Tissue Sections
...... 165
Daehoon Lee, ShanXiong, and Wen-Cheng Xiong
17
Immunofluorescence Staining with Frozen Mouse
or Chick Embryonic Tissue Sections
................................ 175
Hui
Wang and Michael P. Matise
18 ß-Galactosidase
Staining of LacZ Fusion Proteins
in Whole Tissue Preparations
..................................... 189
Margaret A. Cooper and Renting Zhou
19
Chromatin Immunoprecipitation Assay of Brain Tissues
Using
Percoli
Gradient-Purified Nuclei
.............................. 199
Baojin Ding and Daniel L.
Kilpatrick
20
In Vivo Dual Luciferase Reporter Assay with Chick Neural Tube
In
Ovo
Electroporation System
.................................... 211
Hui
Wang and Michael P. Matise
Part III Axon Pathways and Synapsks
21
Growth Cone Collapse Assay
..................................... 221
Xin
Tue,
Alexander I. Son, and RenpingZhou
22
The Stripe Assay: Studying Growth Preference and Axon Guidance
on Binary Choice Substrates In Vitro
............................... 229
Markus
Weschenfelder, Franco Weth,
Bernd Knöll,
and Martin Bastmeyer
23
Microcontact Printing of Substrate-Bound Protein Patterns
for Cell and Tissue Culture
....................................... 247
Martin Fritz and Martin Bastmeyer
24
Semiautomated Analysis of
Dendrite
Morphology in Cell Culture
.......... 261
Eric S. Sweet, Chris L.
Langhammer,
Melinda
К.
Kutzing,
and Bonnie
L.
Firestein
25
Monitoring Synaptic Plasticity by Imaging
AMPA
Receptor Content
and Dynamics on Dendritic Spines
................................. 269
Hiroshi Makino and Bo Li
26
Morphological Analysis of Neuromuscular Junctions
by Immunofluorescent Staining of Whole-Mount Mouse Diaphragms
....... 277
Haitao Wu and Lin
Mei
Contents ix
Part IV Morphological Analyses of the Developing Nervous System
27
Routine Histology Techniques for the Developing
and Adult Central Nervous System
................................. 289
W. Geoffrey McAuliffe
28
Cryosectioning
................................................ 301
Alexander I. Son, Katie Sokolowski, and RenpinjZhou
29
The Golgi-Cox Method
......................................... 313
Gitanjali Das, Kenneth Reuhl, and Renping Zhou
30
Neuroanatomical Tract-Tracing Using Biotinylated Dextran
Amine
......... 323
Nikolai
E. Lazárov
31
Retrograde Tracing Technique for Neonatal Animals
.................... 335
Кещо
Funakoshi, Akira Yoshikawa, and Yoshitoshi Atobe
Index
............................................................... 343
|
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| discipline | Biologie |
| format | Book |
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| indexdate | 2024-07-10T00:37:09Z |
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| isbn | 9781627034432 |
| language | English |
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| publishDate | 2013 |
| publishDateSearch | 2013 |
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| publisher | Humana Press |
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| series | Methods in molecular biology |
| series2 | Methods in molecular biology Springer protocols |
| spelling | Zhou, Renping Verfasser aut Neural development methods and protocols ed. by Renping Zhou ... New York, NY [u.a.] Humana Press 2013 XIII, 347 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 1018 Springer protocols Zellkultur (DE-588)4067547-6 gnd rswk-swf Entwicklung (DE-588)4113450-3 gnd rswk-swf Nervenzelle (DE-588)4041649-5 gnd rswk-swf Methode (DE-588)4038971-6 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Nervenzelle (DE-588)4041649-5 s Zellkultur (DE-588)4067547-6 s DE-604 Entwicklung (DE-588)4113450-3 s Methode (DE-588)4038971-6 s b DE-604 Methods in molecular biology 1018 (DE-604)BV035362695 1018 Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025978127&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Zhou, Renping Neural development methods and protocols Methods in molecular biology Zellkultur (DE-588)4067547-6 gnd Entwicklung (DE-588)4113450-3 gnd Nervenzelle (DE-588)4041649-5 gnd Methode (DE-588)4038971-6 gnd |
| subject_GND | (DE-588)4067547-6 (DE-588)4113450-3 (DE-588)4041649-5 (DE-588)4038971-6 (DE-588)4143413-4 |
| title | Neural development methods and protocols |
| title_auth | Neural development methods and protocols |
| title_exact_search | Neural development methods and protocols |
| title_full | Neural development methods and protocols ed. by Renping Zhou ... |
| title_fullStr | Neural development methods and protocols ed. by Renping Zhou ... |
| title_full_unstemmed | Neural development methods and protocols ed. by Renping Zhou ... |
| title_short | Neural development |
| title_sort | neural development methods and protocols |
| title_sub | methods and protocols |
| topic | Zellkultur (DE-588)4067547-6 gnd Entwicklung (DE-588)4113450-3 gnd Nervenzelle (DE-588)4041649-5 gnd Methode (DE-588)4038971-6 gnd |
| topic_facet | Zellkultur Entwicklung Nervenzelle Methode Aufsatzsammlung |
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