The role of Fluoxetine against preneoplastic lesions and tumors in colon tissue:
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| Format: | Thesis Book |
| Language: | English |
| Published: |
2013
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| Subjects: | |
| Online Access: | Volltext Inhaltsverzeichnis |
| Item Description: | Zsfassung in dt. und engl. Sprache |
| Physical Description: | 104 Bl. graph. Darst. |
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| 100 | 1 | |a Kannen Cardoso, Vinicius |d 1983- |e Verfasser |0 (DE-588)1034306650 |4 aut | |
| 245 | 1 | 0 | |a The role of Fluoxetine against preneoplastic lesions and tumors in colon tissue |c von Vinicius Kannen Cardoso |
| 246 | 1 | 3 | |a Die Rolle von Fluoxetin gegen präneoplastische Läsionen und Tumoren im Darmgewebe |
| 264 | 1 | |c 2013 | |
| 300 | |a 104 Bl. |b graph. Darst. | ||
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| 502 | |a Würzburg, Univ., Diss., 2013 | ||
| 650 | 0 | 7 | |a Fluoxetin |0 (DE-588)4255786-0 |2 gnd |9 rswk-swf |
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Record in the Search Index
| _version_ | 1804150303824543744 |
|---|---|
| adam_text | IMAGE 1
INDEX
ABBREVIATIONS 8
ABSTRACT 10
ZUSAMMENFASSUNG 11
1 INTRODUCTION 13
1.1 FLUOXETINE 13
1.2 FLUOXETINE AND OXIDATIVE STRESS GENERATION 13
1.3 FLUOXETINE AND TUMORS 14
1.4 INCIDENCE OF COLON TUMORS 15
1.4.1 ADENOMA-CARCINOMA SEQUENCE MODEL 16
1.4.1 COLON PRENEOPLASTIC LESIONS, ABERRANT CRYPT FOCI, OR DYSPLASIA 16
1.4.2 COLON PRENEOPLASTIC LESIONS AND STROMAL AREAS 17
1.4.3 TUMOR METABOLISM 18
1.4.4 HYPOXIA, METABOLISM, AND INTRACELLULAR PH 19
2 AIMS 21
3 MATERIAL AND METHODS 22
3.1 FLUOXETINE STOCK SOLUTION PREPARATION 22
3.2 FERRIC REDUCING ANTIOXIDANT POWER ASSAY 22
3.3 CELL CULTURE 23
3.3.1 CELL LINES 23
3.3.1.1 V79 HAMSTER FIBROBLAST CELL LINE 23
3.3.1.2 HT29 HUMAN COLORECTAL ADENOCARCINOMA CELL LINE 23
3.3.1.3 CACO-2 HUMAN COLORECTAL ADENOCARCINOMA CELL LINE 24
3.3.1.3.1 EXPERIMENTAL DESIGN I 24
HTTP://D-NB.INFO/1036506991
IMAGE 2
3.3.1.3.2 EXPERIMENTAL DESIGN II 24
3.3.1.3.3 EXPERIMENTAL DESIGN III 24
3.3.2 ANALYTICAL METHODS 25
3.3.2.1 FLOW CYTOMETRY 25
3.3.2.1.1 DETECTION OF REATIVE OXYGEN SPECIES GENERATION 25
3.3.2.1.2 ANNEXIN V/PROPIDIUM IODIDE ASSAY 25
3.3.2.1.3 DETECTION OF SUPEROXIDE 25
3.3.2.1.4 INTRACELLULAR PH ANALYSIS 25
3.3.2.1.5 MITOCHONDRIAL MEMBRANE POTENTIAL ANALYSIS 26
3.3.2.1.6 CELL-CYCLE ANALYSIS 26
3.3.2.2 VIABILITY TEST 26
3.3.2.3 COMET ASSAY 27
3.3.2.4 WESTERN BLOTTING ANALYSIS 28
3.4 ANIMAL EXPERIMENTS 28
3.4.1 WISTAR RATS 29
3.4.2 C57BL/6 MICE 29
3.4.2.1 ANALYTICAL METHODS 29
3.4.2.1.1 HISTOPATHOLOGICAL ANALYSIS 30
3.4.2.1.2 IMMUNOHISTOCHEMISTRY AND IMMUNOFLUORESCENCE 30
3.4.2.2 NONOBESE DIABETIC SEVERE COMBINED IMMUNODEFICIENT MICE 33
3.4.2.2.1 FIRST EXPERIMENT - CACO-2 XENOGRAFT-TUMORS 33
3.4.2.2.2 SECOND EXPERIMENT - HT29 XENOGRAFT-TUMORS 33
3.4.2.2.3 THIRD EXPERIMENT - HT29 XENOGRAFT-TUMORS 34
3.4.2.2.3.1 MONITORING OF XENOGRAFT-TUMORS 34
3.4.2.2.4 ANALYTICAL METHODS 34
IMAGE 3
3.4.2.2.4.1 HISTOPATHOLOGICAL ANALYSIS 34
3.4.2.2.4.2 IMMUNOHISTOCHEMISTRY AND IMMUNOFLUORESCENCE 35
3.4.2.2.4.3 WESTERN BLOTTING ANALYSIS OF XENOGRAFT-TUMOR SAMPLES 35
3.4.2.2.4.4 MILLIPLEX ASSAY FOR HUMAN OXIDATIVE PHOSPHORYLATION AND
PROTEIN
KINASE B/MAMMALIAN TARGET OF RAPAMYCIN PHOSPHOPROTEIN SIGNALING 36
3.4.2.2.4.5 QUANTITATIVE REAL-TIME REVERSE TRANSCRIPTION-POLYMERASE
CHAIN
REACTION 37
3.4.2.2.4.6 BIOCHEMICAL MEASUREMENTS 38
3.4.2.2.4.7 STATISTICAL ANALYSIS 38
4 RESULTS 39
4.1 FLUOXETINE DOES NOT SHOW ANTIOXIDANT, PRO-OXIDANT OR DNA-DAMAGING
POTENTIAL 39
4.2 EFFECTS OF FLUOXETINE TREATMENT IN WISTAR RATS 41
4.2.1 FLUOXETINE REDUCES DYSPLASIA, CRYPTAL PROLIFERATION, AND
PRENEOPLASTIC
ANGIOGENESIS IN DIMETHYLHYDRAZINE-EXPOSED COLON TISSUE 41
4.3 EFFECTS OF FLUOXETINE TREATMENT IN C57BL/6 MICE 43
4.3.1 FLUOXETINE DECREASES DYSPLASIA AND PRENEOPLASTIC ANGIOGENESIS IN
METHYLNITRONITROSOGUANIDINE-EXPOSED COLON TISSUE 43
4.3.2 FLUOXETINE REDUCES COLON PROLIFERATION IN EPITHELIA AND STROMAL
AREAS IN
METHYLNITRONITROSOGUANIDINE-EXPOSED COLON TISSUE 45
4.3.1 FLUOXETINE MODULATES THE EXPRESSION OF NF-ICB-SIGNALING ELEMENTS
IN
METHYLNITRONITROSOGUANIDINE-EXPOSED COLON TISSUE 47
4.3.2 FLUOXETINE CONTROLS ANGIOGENESIS-RELATED MARKERS IN
METHYLNITRONITROSOGUANIDINE-TREATED MICE 49
4.4 EFFECTS OF FLUOXETINE TREATMENT IN COLON TUMORS 52
IMAGE 4
4.4.1 FIRST EXPERIMENT - CACO-2 CELL LINE 52
4.4.1.1 FLUOXETINE REDUCES REACTIVE OXYGEN SPECIES GENERATION IN CACO-2
CELLS
52
4.4.1.2 FLUOXETINE DECREASES INTRACELLULAR PH, MITOCHONDRIAL MEMBRANE
POTENTIAL, AND SUPEROXIDE GENERATION IN HYPOXIA-EXPOSED CACO-2 CELLS 53
4.4.1.3 FLUOXETINE SHRINKS CACO-2 XENOGRAFT-TUMORS BY REDUCING
PROLIFERATION 55
4.4.2 SECOND EXPERIMENT - HT29 CELL LINE 58
4.4.2.1 FLUOXETINE DOES NOT INDUCE REACTIVE OXYGEN SPECIES OR
DNA-DAMAGE,
BUT DELAYS HT29 CELLS AT THE G0/G1 CELL-CYCLE PHASE 58
4.4.2.2 FLUOXETINE REDUCES REACTIVE OXYGEN SPECIES GENERATION IN HT29
CELLS 61
4.4.2.3 FLUOXETINE DECREASES INTRACELLULAR PH, MEMBRANE MITOCHONDRIAL
POTENTIAL, AND SUPEROXIDE GENERATION ARRESTING HYPOXIA-EXPOSED HT29
CELLS AT
THE G0/G1 CELL-CYCLE PHASE 62
4.4.2.4 FLUOXETINE SHRINKS HT29 XENOGRAFT-TUMORS BY REDUCING
PROLIFERATION 65
4.4.3 THIRD EXPERIMENT - HT29 TUMORS 67
4.4.3.1 FLUOXETINE AND 5-FLUOROURACIL HAVE SIMILAR EFFECTS AGAINST HT29
XENOGRAFT-TUMORS 67
4.4.3.1 FLUOXETINE ACTS AGAINST TUMOR METABOLISM AND MOLECULAR SIGNALING
68
5 DISCUSSION 71
5.1 STANDARD-FLUOXETINE DOSES SHOW SAFETY IN TOXICOLOGICAL TESTS 71
5.2 FLUOXETINE REDUCES COLON DYSPLASIA TAKING PRENEOPLASTIC ANGIOGENESIS
UNDER
CONTROL 72
5.3 FLUOXETINE TAKES TUMOR METABOLISM UNDER CONTROL TO REDUCE ITS GROWTH
75
6 CONCLUSION 79
7 REFERENCES 80
IMAGE 5
8 ACKNOWLEDGEMENT
9 CURRICULUM VITAE
10 AFFIDAVIT
|
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| spelling | Kannen Cardoso, Vinicius 1983- Verfasser (DE-588)1034306650 aut The role of Fluoxetine against preneoplastic lesions and tumors in colon tissue von Vinicius Kannen Cardoso Die Rolle von Fluoxetin gegen präneoplastische Läsionen und Tumoren im Darmgewebe 2013 104 Bl. graph. Darst. txt rdacontent n rdamedia nc rdacarrier Zsfassung in dt. und engl. Sprache Würzburg, Univ., Diss., 2013 Fluoxetin (DE-588)4255786-0 gnd rswk-swf Magenkrebs (DE-588)4036951-1 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Fluoxetin (DE-588)4255786-0 s Magenkrebs (DE-588)4036951-1 s DE-604 Erscheint auch als Online-Ausgabe urn:nbn:de:bvb:20-opus-77589 https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-77589 Resolving-System kostenfrei Volltext DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025961583&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Kannen Cardoso, Vinicius 1983- The role of Fluoxetine against preneoplastic lesions and tumors in colon tissue Fluoxetin (DE-588)4255786-0 gnd Magenkrebs (DE-588)4036951-1 gnd |
| subject_GND | (DE-588)4255786-0 (DE-588)4036951-1 (DE-588)4113937-9 |
| title | The role of Fluoxetine against preneoplastic lesions and tumors in colon tissue |
| title_alt | Die Rolle von Fluoxetin gegen präneoplastische Läsionen und Tumoren im Darmgewebe |
| title_auth | The role of Fluoxetine against preneoplastic lesions and tumors in colon tissue |
| title_exact_search | The role of Fluoxetine against preneoplastic lesions and tumors in colon tissue |
| title_full | The role of Fluoxetine against preneoplastic lesions and tumors in colon tissue von Vinicius Kannen Cardoso |
| title_fullStr | The role of Fluoxetine against preneoplastic lesions and tumors in colon tissue von Vinicius Kannen Cardoso |
| title_full_unstemmed | The role of Fluoxetine against preneoplastic lesions and tumors in colon tissue von Vinicius Kannen Cardoso |
| title_short | The role of Fluoxetine against preneoplastic lesions and tumors in colon tissue |
| title_sort | the role of fluoxetine against preneoplastic lesions and tumors in colon tissue |
| topic | Fluoxetin (DE-588)4255786-0 gnd Magenkrebs (DE-588)4036951-1 gnd |
| topic_facet | Fluoxetin Magenkrebs Hochschulschrift |
| url | https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-77589 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025961583&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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