Molecular insights into the protein disulfide isomerase family:
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| Main Author: | |
|---|---|
| Format: | Thesis Book |
| Language: | English |
| Published: |
2012
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| Subjects: | |
| Online Access: | Volltext Inhaltsverzeichnis |
| Item Description: | Zsfassung in dt. und engl. Sprache. - Online erschienen: 2013 |
| Physical Description: | 158 Bl. Ill., graph. Darst. |
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| 100 | 1 | |a Kober, Franz-Xaver Wilhelm |d 1981- |e Verfasser |0 (DE-588)1033776793 |4 aut | |
| 245 | 1 | 0 | |a Molecular insights into the protein disulfide isomerase family |c von Franz-Xaver Wilhelm Kober |
| 246 | 1 | 3 | |a Molekulare Mechanismen der Protein Disulfid Isomerase Familie |
| 264 | 1 | |c 2012 | |
| 300 | |a 158 Bl. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
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| 500 | |a Zsfassung in dt. und engl. Sprache. - Online erschienen: 2013 | ||
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Record in the Search Index
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| adam_text | IMAGE 1
D TABLE OF CONTENTS
A ACKNOWLEDGEMENTS - 2
B SUMMARY - ENGLISH - 4
C ZUSAMMENFASSUNG - DEUTSCH - 6
D TABLE OF CONTENTS - 8
1. INTRODUCTION - 1 0
1.1 ON PROTEINS, STRUCTURES AND ENERGY LANDSCAPES - 10
1.2 REDOX CHEMISTRY AND ITS APPLICATION TO PROTEINS - 1 6
1.3 ASSISTANTS IN FOLDING: THE PROTEIN DISULFIDE ISOMERASE FAMILY - 2 0
1.3.1 THE ARCHITECTURE O F PROTEIN DISULFIDE ISOMERASE (PDIA1) - 22
1.3.2. THE PDI PROTEIN FAMILY - 26
2. MATERIALS & METHODS - 28
2.1 MOLECULAR BIOLOGY - 28
2.1.1 SEPARATION O F DNA MOLECULES BY SIZE - 28
2.1.2 EXTRACTION O F DNA FROM AGAROSE GELS - 29
2.1.3 PURIFICATION O F PLASMID DNA - 29
2.1.4 DIGESTION WITH RESTRICTION ENDONUCLEASES - 30
2.1.5 POLYMERASE CHAIN REACTION AND SITE DIRECTED MUTAGENESIS - 30
2.1.6 SEQUENCING O F DNA - 31
2.1.7 CULTIVATION AND STORAGE OF E. COLI STRAINS - 32
2.1.8 TRANSFORMATION O F E. COLI - 32
2.1.9 LARGE SCALE LYSIS O F E. COLI CELLS - 33
2.2 BIOINFORMATICS - 35
2.2.1 UNIPROTKB DATABASE - 35
2.2.2 EXPASY TOOLS AND PROTPARAM - 35
2.2.3 CLUSTALW - 3 5
2.2.4 GENERUNNER - 36
2.2.5 PYMOL - 36
2.2.6 NCBI DATABASE AND TOOLS - 36
2.2.7 MOSFLM / XDS - 3 7
2.2.8 CCP4 PROGRAM SUITE - 37
2.2.9 PHENIX PROGRAM SUITE - 37
2.2.10 HYDROPRO - 38
- 8 -
HTTP://D-NB.INFO/1037169115
IMAGE 2
2.3 PROTEIN BIOCHEMISTRY - 39
2.3.1 DETERMINATION O F PROTEIN CONCENTRATION - 39
2.3.2 SEPARATION O F PROTEINS BY SDS-POLYACRYLAMIDE GEL ELECTROPHORESIS
- 39
2.3.3 COOMASSIE STAINING O F POLYACRYLAMIDE GELS - 4 0
2.3.4. PROTEIN PURIFICATION PROTOCOLS - 4 1
2.3.5 REDUCED CARBOXYMETHYLATION O F MODEL SUBSTRATES - 4 6
2.3.6 PREPARATION O F APO-RIBOFLAVIN BINDING PROTEIN - 46
2.3.7 PREPARATION O F DI-EOSINE-GLUTHATIONE (DI-E-GSSG) - 4 7
2.3.8 FLUORESCENCE SELF-QUENCHING (FSQ) ASSAY - 4 7
2.3.9 RIBOFLAVING QUENCHING ASSAY - 4 8
2.3.10 ISOTHERMAL TITRATION CALORIMETRY (ITC) - 4 9
2.3.11 CIRCULAR DICHROISM SPECTROSCOPY (CD SPECTROSCOPY) - 5 0
2.3.12 DYNAMIC LIGHT SCATTERING (DLS) - 51
2.3.13 CRYSTALLIZATION OF PROTEINS - 52
2.3.13 DIFFRACTION EXPERIMENTS - 53
2.3.14 DATA PROCESSING - 5 4
2.3.15 STRUCTURE SOLUTION - 55
2.4 MATERIALS - 58
2.4.1 LIST O F PRIMERS - 58
2.4.2 LIST OF EXPRESSION VECTORS - 61
2.4.3 EQUIPMENT - 62
2.4.4 CONSUMABLES - 63
3.4.5 CHEMICALS AND SOLVENTS - 6 3
3. RESULTS & DISCUSSION - 6 5
3.1 THE MULTISTATE-EQUILIBRIUM O F YEAST PDI - 65
3.2 CRYSTALLIZATION O F PDI FAMILY MEMBERS - 79
3.3 FLUORESCENCE-BASED ASSAYS FOR ACTIVITY MEASUREMENTS O F PDI FAMILY
PROTEINS - 102
3.4 SUBSTRATE RECOGNITION O F THE PDI FAMILY - 122
4. SYNOPSIS & OUTLOOK - 140
5. REFERENCES - 143
E LIST OF ABBREVATIONS - 151
F LIST OF FIGURES - 153
G LIST OF PUBLICATIONS AND CONGRESS CONTRIBUTIONS - 155
H CURRICULUM VITAE - 156
- 9 -
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| language | English |
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| spelling | Kober, Franz-Xaver Wilhelm 1981- Verfasser (DE-588)1033776793 aut Molecular insights into the protein disulfide isomerase family von Franz-Xaver Wilhelm Kober Molekulare Mechanismen der Protein Disulfid Isomerase Familie 2012 158 Bl. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Zsfassung in dt. und engl. Sprache. - Online erschienen: 2013 Würzburg, Univ., Diss., 2012 Biochemie (DE-588)4006777-4 gnd rswk-swf Protein-Disulfid-Isomerase (DE-588)4256460-8 gnd rswk-swf Disulfidbrücke (DE-588)4275810-5 gnd rswk-swf Proteinfaltung (DE-588)4324567-5 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Disulfidbrücke (DE-588)4275810-5 s Protein-Disulfid-Isomerase (DE-588)4256460-8 s Proteinfaltung (DE-588)4324567-5 s Biochemie (DE-588)4006777-4 s DE-604 Erscheint auch als Online-Ausgabe urn:nbn:de:bvb:20-opus-72144 https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-72144 Resolving-System kostenfrei Volltext DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025938920&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Kober, Franz-Xaver Wilhelm 1981- Molecular insights into the protein disulfide isomerase family Biochemie (DE-588)4006777-4 gnd Protein-Disulfid-Isomerase (DE-588)4256460-8 gnd Disulfidbrücke (DE-588)4275810-5 gnd Proteinfaltung (DE-588)4324567-5 gnd |
| subject_GND | (DE-588)4006777-4 (DE-588)4256460-8 (DE-588)4275810-5 (DE-588)4324567-5 (DE-588)4113937-9 |
| title | Molecular insights into the protein disulfide isomerase family |
| title_alt | Molekulare Mechanismen der Protein Disulfid Isomerase Familie |
| title_auth | Molecular insights into the protein disulfide isomerase family |
| title_exact_search | Molecular insights into the protein disulfide isomerase family |
| title_full | Molecular insights into the protein disulfide isomerase family von Franz-Xaver Wilhelm Kober |
| title_fullStr | Molecular insights into the protein disulfide isomerase family von Franz-Xaver Wilhelm Kober |
| title_full_unstemmed | Molecular insights into the protein disulfide isomerase family von Franz-Xaver Wilhelm Kober |
| title_short | Molecular insights into the protein disulfide isomerase family |
| title_sort | molecular insights into the protein disulfide isomerase family |
| topic | Biochemie (DE-588)4006777-4 gnd Protein-Disulfid-Isomerase (DE-588)4256460-8 gnd Disulfidbrücke (DE-588)4275810-5 gnd Proteinfaltung (DE-588)4324567-5 gnd |
| topic_facet | Biochemie Protein-Disulfid-Isomerase Disulfidbrücke Proteinfaltung Hochschulschrift |
| url | https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-72144 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025938920&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT koberfranzxaverwilhelm molecularinsightsintotheproteindisulfideisomerasefamily AT koberfranzxaverwilhelm molekularemechanismenderproteindisulfidisomerasefamilie |