Cilia: B
Saved in:
| Format: | Book |
|---|---|
| Language: | English |
| Published: |
Amsterdam [u.a.]
Elsevier
2013
|
| Edition: | 1. ed. |
| Series: | Methods in enzymology
525 |
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | LVI, 422 S., [8] Bl. Ill., graph. Darst. |
| ISBN: | 9780123979445 0123979447 |
Staff View
MARC
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Record in the Search Index
| _version_ | 1804150206301732864 |
|---|---|
| adam_text | CONTENTS
Contributors
xi
Preface
xvii
Volume in Series
xxi
1.
Non-Motile Primary Cilia as Fluid-Shear Stress Mechanosensors
1
Surya M. Nauli, Xingjian Jin, Wissam A. AbouAlaiwi, Wassim El-Jouni,
Xuefeng
Su,
and Jing Zhou
1.
Introduction
2
2.
Identification of Primary Cilium
3
3.
Mechanosensory Cilia
6
4.
Summary
17
Acknowledgments
17
References
17
2.
Biomechanical Measurement of Kinocilium
21
Corrie Spoon and
Wally
Grant
1.
Introduction
22
2.
Sample Preparation and Measurement
30
3.
Determining Mechanical Properties from Experimental Measurements
34
4.
Method Discussion
38
Acknowledgments
41
References
41
3.
Analysis of Primary Cilia in Directional Cell Migration
in
Fibroblasts
45
Seren
T.
Christensea Iben
R.
Veland,
Albrecht Schwab,
Michael Cammer,
and Peter Satir
1.
Introduction
46
2.
Localization of Primary Cilia by Immunofluorescence Microscopy A1
3.
Cell Migration and Orientation of Primary Cilia in Scratch Assays
50
4.
Cell Migration and Ciliary Signaling in
Micropipette
Assays
53
5.
Summary 57
Acknowledqments
57
References
57
vi
Contents
4.
Sperm Patch-Clamp
59
Polina
Lishko, David E. Clapham, Betsy
Navarro,
and Yuriy Kirichok
1.
Introduction
60
2.
Equipment
65
3.
Isolation of Spermatozoa for Patch-Clamp Experiments
67
4.
Patch-Clamp Recording
71
5.
Potential Pitfalls Associated with Sperm Patch Clamp
79
6.
Conclusions
81
Acknowledgments
81
References
81
5.
Laser Trap Measurements of
Flagellar
Membrane Motility
85
William H. Guilford and Robert A. Bloodgood
1.
Introduction
86
2.
Laser Trap Overview
88
3.
Measuring
Flagellar
Membrane Transport
91
Acknowledgments
105
References
105
6.
Probing Ciliogenesis Using Micropatterned Substrates
109
Amandine Pitaval, Andreas Christ, Alexis Curtet, Qingzong Tseng,
and Manuel
Théry
1.
Introduction
110
2.
Micropatterned Substrates Fabrication
112
3.
Cells Plating on Micropatterned Slides
119
4.
Cell Fixation and Immunofluorescence
122
5.
Automated Image Acquisition
124
6.
Automated Image Analysis
125
Acknowledgments
129
References
129
7.
Fluorescent Imaging of
Drosophila
melanogaster Sperm
in the Reproductive Tract: A New Model of
Flagellar
Motility
131
Xiangyi
Lu
1.
Introduction
132
2.
Imaging the Fly Sperm
140
3.
The Fly Sperm as a Model for Studying
Flagellar
Motility
146
Contents
vii
Acknowledgments
147
References
147
8.
Visualization of Mouse Nodal Cilia and Nodal Flow
149
Shigenori Nonaka
1.
Introduction and Historical Overview
149
2.
Tools and Solutions
151
3.
Dissection Procedures
152
4.
Observation by Light Microscopy
154
5.
Discussion
156
References
156
9.
Dissecting the Functional Interplay Between the TOR
Pathway and the Cilium in Zebrafish
159
Shiaulou Yuan,
Lu
Zhao, and Zhaoxia Sun
1.
Introduction
160
2.
Manipulation of the TOR Pathway in Zebrafish
161
3.
Analysis of Cilia Length in Zebrafish
167
4.
Cilia Motility and Fluid Flow Dynamics in the KV
174
5.
Assessing for Laterality Defects in Zebrafish
181
6.
Utilizing Chlamydomonas to Assay for TOR-Mediated Cilia Length Control
181
7.
Summary
187
Acknowledgments
188
References
188
10.
Using Xenopus Skin to Study Cilia Development and Function
191
Michael E. Werner and Brian J. Mitchell
1.
Introduction
192
2.
Molecular Manipulation of Xenopus Ciliated Epithelia
192
3.
Visualization of Cilia in Xenopus Embryos
199
4.
Embryological Manipulations Useful for the Analysis of Ciliated Epithelia
207
5.
Conclusion
215
Acknowledgments
215
References
215
11.
Zebrafish Cilia
219
lain
Drummond
and Christina Austin-Tse
1.
Introduction
220
2.
Molecular Approaches to Ciliogenesis in Zebrafish
221
viii Contents
3.
Wholemount Confocallmaging of Zebrafish
Cilia
231
4.
Electron
Microscopy Methods for Fish Cilia
237
5.
Live Imaging of Zebrafish Cilia Using arll3b:GFP Expression
239
6.
Summary
240
Acknowledgments
241
References
241
12.
Analysis of Ciliary Assembly and Function in
Planaria
245
Panteleímon
Rompolas, Juliette Azimzadeh, Wallace F. Marshall,
and Stephen M. King
1.
Introduction
246
2.
Maintaining
Planarians
in the Lab
247
3.
Genetic Manipulation of
Planaria
250
4.
Imaging
Planarian
Cilia
254
5.
Planaria
Gliding and Ciliary Motility Assays
259
6.
Summary
262
References
263
13.
Discovery and Functional Evaluation of Ciliary Proteins
in Tetrahymena thermophila
265
Jacek
Gaertig,
Dorota Wloga,
Krishna Kumar Vasudevan, Mayukh Guha,
and William
Dentier
1.
Introduction
266
2.
Cell Culture
267
3.
Deciliation, Purification, and Fractionation of Cilia
269
4.
Localization of Proteins in Tetrahymena
273
5.
Phenotypic Studies on Live Ciliary Mutants
280
6.
Summary
282
Acknowledgments
282
References
282
14.
Analysis of Ciliogenesis in Primary Culture Mouse
Trachéal
Epithelial Cells
285
Eszter
К.
Vladar
and Steven
L. Brody
1.
Introduction
286
2.
Generation of MTEC Cultures
287
3.
Staging Ciliogenesis in MTEC Preparations
296
4.
Assessment of Ciliogenesis and MCCs by Transmission Electron Microscopy
300
5.
Gene Transfer, Drug Treatment, and FACS of MTEC Preparations
303
Contents ix
б.
Summary
308
Acknowledgments
308
References
308
15.
Isolation of Mammalian Primary Cilia
311
Hiroaki Ishikawa and Wallace F. Marshall
1.
Introduction
312
2.
Cell Culture
314
3.
Isolation of Mammalian Primary Cilia
316
4.
Protein Correlation Profiling
319
5.
Immunofluorescence Microscopy of Primary Cilia
320
6.
Summary
322
Acknowledgments
323
References
323
16.
Finding Ciliary Genes: A Computational Approach
327
Johan Henriksson,
Brian P.
Piasecki, Kristina
Lend, Thomas R.
Bürglin,
and Peter
Swoboda
1.
Introduction
328
2.
Choice of Bioinformatics Software
331
3.
Retrieving Genome (Sequence) Data
332
4.
Finding Orthologous Genes
333
5.
Searching Sequences for Regulatory Elements
336
6.
Scoring Candidate Genes
339
7.
Summary and Discussion
345
Acknowledgments
347
References
347
17.
Chemical Screening Methods for
Flagellar
Phenotypes
in Chlamydomonas
351
Prachee Avasthi and Wallace F. Marshall
1.
Introduction
352
2.
Screen Design Considerations
353
3.
Flagellar
Length Assay
356
4.
Deflagellation Assay
357
5.
Motility Assay
358
6.
Nonflagellar Phenotypes
359
7.
Data Analysis
362
8.
Target Validation and Identification
366
X
Contents
9.
Summary
367
Acknowledgments
368
References
368
18.
Centrosome Isolation and Analysis by Mass Spectrometry-Based
Proteomics
371
Lis
Jakobsen, Jacob Morville Schroder,
Katja
M.
Larsen, Emma
Lundberg,
and Jens S. Andersen
1.
Introduction
372
2.
Centrosome Isolation
374
3.
Centrosome Protein Identification
380
4.
Probing Centrosome Protein Properties by Quantitative Proteomics
386
5.
Summary
390
Acknowledgments
390
References
391
Author Index
395
Subject Index
413
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| language | English |
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| series | Methods in enzymology |
| series2 | Methods in enzymology |
| spelling | Cilia B ed. by Wallace F. Marshall 1. ed. Amsterdam [u.a.] Elsevier 2013 LVI, 422 S., [8] Bl. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in enzymology 525 Methods in enzymology ... Proteindesign (DE-588)4346801-9 gnd rswk-swf Zilie (DE-588)4190826-0 gnd rswk-swf Zilie (DE-588)4190826-0 s Proteindesign (DE-588)4346801-9 s DE-604 Marshall, Wallace F. Sonstige oth (DE-604)BV040914191 2 Methods in enzymology 525 (DE-604)BV000000938 525 Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025893298&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Cilia Methods in enzymology Proteindesign (DE-588)4346801-9 gnd Zilie (DE-588)4190826-0 gnd |
| subject_GND | (DE-588)4346801-9 (DE-588)4190826-0 |
| title | Cilia |
| title_auth | Cilia |
| title_exact_search | Cilia |
| title_full | Cilia B ed. by Wallace F. Marshall |
| title_fullStr | Cilia B ed. by Wallace F. Marshall |
| title_full_unstemmed | Cilia B ed. by Wallace F. Marshall |
| title_short | Cilia |
| title_sort | cilia |
| topic | Proteindesign (DE-588)4346801-9 gnd Zilie (DE-588)4190826-0 gnd |
| topic_facet | Proteindesign Zilie |
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