The role of the proteoglycan versican in high-grade gliomas:
Saved in:
| Main Author: | |
|---|---|
| Format: | Thesis Book |
| Language: | English |
| Published: |
2012
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| Subjects: | |
| Online Access: | Volltext https://nbn-resolving.org/urn:nbn:de:bvb:355-epub-278633 Inhaltsverzeichnis |
| Physical Description: | 126, 8 Bl. Ill., graph. Darst. |
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Record in the Search Index
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|---|---|
| adam_text | IMAGE 1
CONTENTS
ABBREVIATIONS VII
INTRODUCTION 10
1.1 GLIOBLASTOMA MULTIFORME 10
1.1.1 MALIGNANCY CRITERIA IN GBM 11
1.2 EXTRACELLULAR MATRIX 12
1.2.1 STRUCTURE AND FUNCTION O F EXTRACELLULAR MATRIX 12
1.2.2 EXTRACELLULAR MATRIX IN NORMAL BRAIN TISSUE 12
1.2.3 EXTRACELLULAR MATRIX IN BRAIN TUMOURS 13
1.3 VERSICAN 14
1.3.1 THE ROLE O F VERSICAN ISOFORMS IN NORMAL TISSUE AND IN CANCER 16
1.4 QUESTION AND HYPOTHESIS 18
1.4.1 METHODOLOGICAL APPROACH 18
MATERIAL AND METHODS 21
1.5 MATERIALS 21
1.6 METHODS 29
1.6.1 CELL CULTURE 29
1.6.2 CELL COUNT 30
1.7 PREPARING RNA 32
1.7.1 RNA ISOLATION 32
1.7.2 REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION (RT-PCR) 33
1.8 DNA SYNTHESIS AND ANALYSIS 34
1.8.1 POLYMERASE CHAIN REACTION 34
1.8.2 PLASMID AMPLIFICATION IN DH5CC 36
1.8.3 ELECTROPHORESIS 38
1.8.4 GRADIENT PCR 39
1.8.5 QUANTITATIVE PCR 39
1.9 STABLE AND TRANSIENT TRANSFECTION 4 1
1.9.1 DESIGN OFSIRNAS. 41
1.9.2 CALCULATING THE SIRNA AMOUNT. 41
1.9.3 OPTIMIZING SIRNA TRANSFECTION 42
1.9.4 SH-RNA 42
1.9.5 TRANSFECTION O F ADHERENT CELLS 44
V
HTTP://D-NB.INFO/1034750607
IMAGE 2
1.10 PROTEIN 45
1.10.1 PROTEIN ISOLATION TECHNIQUES 45
1.10.2 IMMUNPRECIPITATION 46
1.10.3 BCA ASSAY 4 7
1.10.4 POLYACRYLAMIDGEL ELECTROPHORESIS 48
1.10.5 TRANSFERRING AND VISUALIZING THE PROTEIN 49
1.10.6 IMMUNOCYTOCHEMISTRY 51
L I 0.7 FUNCTIONAL ASSAYS 52
1.10.8 STATISTICAL ANALYSIS 55
RESULTS 57
1.11 SET UP EXPERIMENTS 57
1.11.1 RT-PCR 57
1.11.2 QUANTITATIVE PCR (QPCR) 59
1.11.3 WESTERN BLOT. 59
1.11.4 IMMUNOCYTOCHEMISTRY 62
1.11.5 SIRNA 64
1.12 TRANSFECTION WITH SIVL, SIV2 AND SIV3 69
1.12.1 SEMI-QUANTITATIVE ANALYSIS O F SIVL TRANSFECTION IN RT-PCR 69
1.12.2 SEMI-QUANTITATIVE ANALYSIS O F SIV2 TRANSFECTION IN RT-PCR 72
1.12.3 SEMI-QUANTITATIVE ANALYSIS O F SIV3 TRANSFECTION IN RT-PCR 73
1.12.4 QUANTITATIVE PCR IN SIVL-TRANSFECTED CELLS. 73
1.12.5 PROTEIN REGULATION 75
1.12.6 PROLIFERATION ASSAY. 79
1.12.7 ATTACHMENT ASSAY. 81
1.12.8 MIGRATION ASSAYS 87
DISCUSSION 94
LIST OF FIGURES AND TABLES 104
LIST OF LITERATURE 110
VI
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| spelling | Onken, Julia Sophie 1984- Verfasser (DE-588)1032579544 aut The role of the proteoglycan versican in high-grade gliomas vorgelegt von Julia Sophie Onken 2012 126, 8 Bl. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Regensburg, Univ., Diss., 2013 Glioblastom (DE-588)4157617-2 gnd rswk-swf Proteoglykane (DE-588)4176001-3 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Glioblastom (DE-588)4157617-2 s Proteoglykane (DE-588)4176001-3 s DE-604 Erscheint auch als Online-Ausgabe urn:nbn:de:bvb:355-epub-278633 http://epub.uni-regensburg.de/27863/ Verlag kostenfrei Volltext https://nbn-resolving.org/urn:nbn:de:bvb:355-epub-278633 Resolving-System DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025874398&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Onken, Julia Sophie 1984- The role of the proteoglycan versican in high-grade gliomas Glioblastom (DE-588)4157617-2 gnd Proteoglykane (DE-588)4176001-3 gnd |
| subject_GND | (DE-588)4157617-2 (DE-588)4176001-3 (DE-588)4113937-9 |
| title | The role of the proteoglycan versican in high-grade gliomas |
| title_auth | The role of the proteoglycan versican in high-grade gliomas |
| title_exact_search | The role of the proteoglycan versican in high-grade gliomas |
| title_full | The role of the proteoglycan versican in high-grade gliomas vorgelegt von Julia Sophie Onken |
| title_fullStr | The role of the proteoglycan versican in high-grade gliomas vorgelegt von Julia Sophie Onken |
| title_full_unstemmed | The role of the proteoglycan versican in high-grade gliomas vorgelegt von Julia Sophie Onken |
| title_short | The role of the proteoglycan versican in high-grade gliomas |
| title_sort | the role of the proteoglycan versican in high grade gliomas |
| topic | Glioblastom (DE-588)4157617-2 gnd Proteoglykane (DE-588)4176001-3 gnd |
| topic_facet | Glioblastom Proteoglykane Hochschulschrift |
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