Role of TNF-a [TNF-alpha] on progressive glomerulosclerosis in Alport nephropathy:
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| Main Author: | |
|---|---|
| Format: | Thesis Book |
| Language: | English |
| Published: |
2012
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| Subjects: | |
| Online Access: | Volltext Volltext Inhaltsverzeichnis |
| Physical Description: | 113 Bl. Ill., graph. Darst. 21 cm |
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MARC
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Record in the Search Index
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|---|---|
| adam_text | IMAGE 1
CONTENTS
1. INTRODUCTION 3
1.1 CHRONIC KIDNEY DISEASE 3
1.1.1 DEVELOPMENT O F GLOMERULOSCLEROSIS 4
1.1.2 GLOMERULOSCLEROSIS-ASSOCIATED CHRONIC KIDNEY DISEASES 8
1.2 CONTRIBUTING FACTORS O F DISEASE PROGRESSION IN CKD 9
1.2.1 LEUKOCYTE INFILTRATION IN CKD 10
1.2.2 ROLE OF TUMOR NECROSIS FACTOR-A IN CKD 14
1.2.3 PATTERN RECOGNITION RECEPTORS AND INNATE IMMUNITY 17
1.3 ALPORT SYNDROME 23
1.3.1 STRUCTURE AND DISTRIBUTION O F TYPE IV COLLAGEN 23
1.3.2 GENETICS O F ALPORT SYNDROME : 25
1.3.3 CLINICAL AND PATHOLOGIC FEATURES O F ALPORT NEPHROPATHY 26
1.3.4 ANIMAL MODELS O F ALPORT SYNDROME 27
1.3.5 MOUSE AUTOSOMAL RECESSIVE ALPORT MODEL 27
1.3.6 TREATMENT O F ALPORT SYNDROME 29
2. HYPOTHESIS/OBJECTIVES 30
3. MATERIALS AND METHODS 31
3.1 MATERIALS 31
3.1.1 EQUIPMENT 31
3.1.2 CHEMICALS AND MATERIALS 32
3.1.4 SOLUTIONS 36
3.2 METHODS 39
3.2.1 ANIMAL STUDIES 39
3.2.1.1 HOMING CONDITIONS AND ANIMAL PROCEDURES 39
3.2.1.2 STUDY DESIGN AND EXPERIMENTAL PROCEDURES 39
3.2.1.3 PLASMA AND URINE SAMPLE ANALYSIS 40
3.2.1.4 IMMUNOHISTOCHEMICAL METHODS AND HISTOPATHOLOGICAL EVALUATION 43
3.2.1.4 ELECTRON MICROSCOPY 44
3.2.1.5 TUNEL ASSAY 44
3.2.2 CYTOKINES 45
3.2.3 RNA ANALYSIS 45
3.2.4 CDNA SYNTHESIS AND REAL-TIME RT-PCR (SYBR GREEN) 46
1
HTTP://D-NB.INFO/1028657749
IMAGE 2
3.2.5 FLOW CYTOMETRY 48
3.2.6 ISOLATION O F RENAL MACROPHAGES 48
3.2.7 PROTEIN ISOLATION AND WESTERN BLOTTING 49
3.2.8 IN VITRO STUDIES 49
3.2.6 STATISTICAL ANALYSIS 50
4. RESULTS 51
4.1 ENDOGENOUS FACTORS CONTRIBUTING TO ALPORT NEPHROPATHY 51
4.1.1 LEUKOCYTE INFILTRATION OF ALPORT NEPHROPATHY IN COL4A3-/- MICE 51
4.1.2 TNF-A EXPRESSION IN COL4A3-/- MICE WITH ALPORT NEPHROPATHY 53
4.1.3 TNF-A EXPRESSION IN INTRARENAL LEUKOCYTES O F COL4A3-/- MICE 54
4.1.4 TNF-A BLOCKADE PROLONGS LIFE SPAN O F COL4A3-/- MICE 56
4.1.5 TNF-A BLOCKADE IMPROVES RENAL FUNCTION IN COL4A3-L- MICE 57
4.1.6 TNF-A BLOCKADE PROTECTS COL4A3-L- MICE FROM GLOMERULOSCLEROSIS 58
4.1.7 TNF-A BLOCKADE PREVENTS PODOCYTE LOSS IN COL4A3-/- MICE 61
4.2 EXOGENOUS FACTORS THAT ACCELERATE THE PROGRESSION O F ALPORT
NEPHROPATHY 65
4.2.1 EFFECT O F INNATE IMMUNITY ON RENAL PARENCHYMAL CELLS 65
4.2.2 MACROPHAGE ACTIVATION VIA TLRS IN ALPORT NEPHROPATHY 67
4.2.2.1 EFFECT O F LPS OR CPG-DNA ON LIFE SPAN O F COL4A3-/- MICE 67
4.2.2.2 CPG-DNA, BUT NOT LPS, AGGRAVATES ALPORT NEPHROPATHY 68
4.2.2.3 CPG-DNA INCREASES ACTIVATED RENAL MACROPHAGES IN COL4A3-L- MICE
...74
4.2.2.4 CPG-DNA AND LPS MODULATE THE EXPRESSION O F MACROPHAGE MARKERS
.77
4.2.2.5 EFFECT O F CPG-DNA ON ALPORT NEPHROPATHY IS MEDIATED BY TNF-A 77
5. DISCUSSION 82
6. SUMMARY AND CONCLUSION 90
7. ZUSAMMENFASSUNG 91
8. REFERENCES 92
9. ABBREVIATIONS 106
CURRICULUM VITAE 110
2
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| physical | 113 Bl. Ill., graph. Darst. 21 cm |
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| spelling | Ryu, Mi 1980- Verfasser (DE-588)1028651953 aut Role of TNF-a [TNF-alpha] on progressive glomerulosclerosis in Alport nephropathy vorgelegt von Mi Ryu 2012 113 Bl. Ill., graph. Darst. 21 cm txt rdacontent n rdamedia nc rdacarrier München, Univ., Diss., 2012 (DE-588)4113937-9 Hochschulschrift gnd-content Erscheint auch als Online-Ausgabe urn:nbn:de:bvb:19-148917 https://nbn-resolving.org/urn:nbn:de:bvb:19-148917 Resolving-System kostenfrei Volltext http://edoc.ub.uni-muenchen.de/14891/ Verlag kostenfrei Volltext DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025780660&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Ryu, Mi 1980- Role of TNF-a [TNF-alpha] on progressive glomerulosclerosis in Alport nephropathy |
| subject_GND | (DE-588)4113937-9 |
| title | Role of TNF-a [TNF-alpha] on progressive glomerulosclerosis in Alport nephropathy |
| title_auth | Role of TNF-a [TNF-alpha] on progressive glomerulosclerosis in Alport nephropathy |
| title_exact_search | Role of TNF-a [TNF-alpha] on progressive glomerulosclerosis in Alport nephropathy |
| title_full | Role of TNF-a [TNF-alpha] on progressive glomerulosclerosis in Alport nephropathy vorgelegt von Mi Ryu |
| title_fullStr | Role of TNF-a [TNF-alpha] on progressive glomerulosclerosis in Alport nephropathy vorgelegt von Mi Ryu |
| title_full_unstemmed | Role of TNF-a [TNF-alpha] on progressive glomerulosclerosis in Alport nephropathy vorgelegt von Mi Ryu |
| title_short | Role of TNF-a [TNF-alpha] on progressive glomerulosclerosis in Alport nephropathy |
| title_sort | role of tnf a tnf alpha on progressive glomerulosclerosis in alport nephropathy |
| topic_facet | Hochschulschrift |
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