Boundary lubricants in osteoarthritic synovial fluid:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
Gießen, Lahn
VVB Laufersweiler Verlag
2012
|
| Ausgabe: | 1. Aufl. |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XIII, 144 S. graph. Darst. |
| ISBN: | 9783835959521 |
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| 264 | 1 | |a Gießen, Lahn |b VVB Laufersweiler Verlag |c 2012 | |
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| 338 | |b nc |2 rdacarrier | ||
| 502 | |a Zugl.: Gießen, Univ., Diss., 2012 | ||
| 650 | 7 | |a osteoarthritis |2 cabt | |
| 650 | 7 | |a arthritis |2 cabt | |
| 650 | 7 | |a joint diseases |2 cabt | |
| 650 | 7 | |a synovial fluid |2 cabt | |
| 650 | 7 | |a lubricants |2 cabt | |
| 650 | 7 | |a materials |2 cabt | |
| 650 | 7 | |a techniques |2 cabt | |
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Datensatz im Suchindex
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| adam_text | IMAGE 1
TABLE O F CONTENTS
I TABLE O F CONTENTS
I TABLE O F CONTENTS I
II LIST O F FIGURES V
III LIST O F TABLES VII
IV LIST O F ABBREVIATIONS VIII
V SUMMARY XI
VI ZUSAMMENFASSUNG XII
1. INTRODUCTION 1
1.1 OSTEOARTHRITIS (OA) 1
1.1.1 SYMPTOMS AND RISK FACTORS O F OA 1
1.1.2 DIAGNOSIS AND THERAPY 2
1.1.3 PATHOPHYSIOLOGY O F O A 3
1.1.3.1 ARTICULAR CARTILAGE 4
1.1.3.2 SYNOVIUM 5
1.1.3.3 SUBCHONDRAL BONE 6
1.1.3.4 SYNOVIAL FLUID 6
1.1.4 ANIMAL MODELS O F OA 7
1.2 RHEUMATOID ARTHRITIS (RA) 8
1.3 THE BOUNDARY LUBRICATION SYSTEM O F ARTICULAR JOINTS 8
1.3.1 LUBRICIN 9
1.3.1.1 FUNCTION O F LUBRICIN IN O A 9
1.3.2 SURFACTANT PROTEINS 10
1.3.3 HYALURONIC ACID 11
1.3.4 LIPIDS 12
1.3.4.1 PHOSPHOLIPIDS 12
1.3.4.1.1 BOUNDARY LUBRICATION PROVIDED BY PL 13
1.3.4.1.2 PHOSPHOLIPIDS CONTAINING CHOLINE 16
1.3.4.1.3 PHOSPHATIDYLETHANOLAMINE AND PE-BASED PLASMALOGEN 17
1.3.4.1.4 PHOSPHATIDYLSERINE 18
I
HTTP://D-NB.INFO/1028502877
IMAGE 2
TABLE O F CONTENTS
1.3.4.1.5 PHOSPHATIDYLINOSITOL 19
1.3.4.1.6 PHOSPHATIDYLGLYCEROL 20
1.3.4.1.7 SPHINGOLIPIDS 20
2. AIM O F THE STUDY 2 3
3. MATERIAL AND METHODS 24
3.1 MATERIALS 24
3.1.1 TECHNICAL EQUIPMENT 24
3.1.2 CONSUMABLES 26
3.1.3 REAGENTS 2 7
3.1.4 REAGENT KITS 30
3.1.5 ANALYSIS SOFTWARE 31
3.1.6 BUFFERS AND SOLUTIONS 31
3.1.7 SYNOVIAL FLUID 34
3.1.7.1 HUMAN SYNOVIAL FLUID 34
3.1.7.2 CANINE SYNOVIAL FLUID 37
3.1.7.3 EQUINE SYNOVIAL FLUID 38
3.1.8 HUMAN CELLS AND TISSUES 38
3.1.8.1 HUMAN FIBROBLAST-LIKE SYNOVIOCYTES 38
3.1.8.2 HUMAN TISSUES 38
3.2 METHODS : 39
3.2.1 SAMPLING O F SYNOVIAL FLUID 39
3.2.2 ISOLATION O F MICROPARTICLES 39
3.2.3 EXTRACTION O F LIPIDS 39
3.2.4 SOLID PHASE EXTRACTION 4 0
3.2.5 PHOSPHOLIPID MASS SPECTROMETRY 41
3.2.6 ESI-MS/MS DATA CORRECTION 41
3.2.6.1 ENZYME-LINKED IMMUNOSORBENT ASSAY FOR P-2-MICROGLOBULIN 41
3.2.6.2 UREA DETERMINATION 42
3.2.7 CULTURE O F HUMAN FIBROBLAST-LIKE SYNOVIOCYTES 4 2
3.2.7.1 ISOLATION O F FIBROBLAST-LIKE SYNOVIOCYTES 42
II
IMAGE 3
TABLE O F CONTENTS
3.2.7.2 EFFECT O F VARIOUS CELL CULTURE CONDITIONS 4 3
3.2.7.3 PREPARATION O F CELL CULTURE MEDIA FOR MS ANALYSIS 44
3.2.8 SF PREPARATION FOR GEL ELECTROPHORESIS 44
3.2.8.1 HYALURONIDASE TREATMENT 44
3.2.8.2 ALBUMIN DEPLETION 4 4
3.2.8.3 PROTEIN QUANTIFICATION 4 4
3.2.9 SEPARATION O F PROTEINS WITH GEL ELECTROPHORESIS 45
3.2.10 WESTERN BLOT 46
3.2.10.1 PROTEIN BLOTTING 46
3.2.10.2 PROTEIN DETECTION 4 6
3.2.11 TWO-DIMENSIONAL GEL ELECTROPHORESIS 4 7
3.2.12 PROTEIN IDENTIFICATION (MALDI-TOF) 4 7
3.2.13 RNA ISOLATION 4 8
3.2.13.1 RNA ISOLATION FROM SYNOVIAL TISSUE AND CELLS 4 8
3.2.13.2 RNA ISOLATION FROM CARTILAGE 49
3.2.14 REVERSE TRANSCRIPTION REACTION 4 9
3.2.15 SEMI-QUANTITATIVE POLYMERASE CHAIN REACTION 50
3.2.16 DNA AGAROSE GEL ELECTROPHORESIS 51
3.2.17 STATISTICAL ANALYSIS O F DATA 52
4. RESULTS 53
4.1 ESTIMATION O F UNDILUTED SF VOLUME 53
4.1.1 ELISA FOR ^ - M I C R O G L O B U L I N S 53
4.1.2 UREA CONCENTRATION IN SERUM AND SF 54
4.2 ANALYSIS O F PL COMPOSITION O F HUMAN SF 56
4.2.1 P L CONTENT IN HEALTHY SF FROM HUMAN CADAVERS 56
4.2.2 COMPOSITION O F PL IN MICROPARTICLES PRESENT IN HUMAN SF 56
4.2.3 COMPOSITION O F PL CLASSES IN HUMAN SF 58
4.2.4 COMPOSITION O F PL SPECIES IN HUMAN SF 59
4.3 PHOSPHOLIPID COMPOSITION O F HEALTHY AND O A CANINE SF 78
4.4 EFFECT O F JOINT DISTRACTION ON COMPOSITION O F PL IN SF 79
111
IMAGE 4
TABLE O F CONTENTS
4.5 COMPOSITION O F PL IN VARIOUS MAMMALIAN SPECIES 80
4.6 FIBROBLAST-LIKE SYNOVIOCYTES CELL CULTURE 82
4.6.1 OPTIMISATION O F FLS CULTURE CONDITIONS 82
4.6.2 DEVELOPMENT O F A METHOD FOR P L DETECTION IN CELL CULTURE MEDIA
83
4.7 DETECTION O F SURFACTANT PROTEINS IN HUMAN KNEE JOINTS 85
4.7.1 WESTERN BLOT ANALYSIS O F SURFACTANT PROTEINS IN HUMAN SF 85
4.7.2 MALDI-TOF/MS PROTEIN IDENTIFICATION 86
4.7.3 EXPRESSION O F SURFACTANT PROTEINS 87
4.7.3.1 TOTAL RNA ISOLATION FROM HUMAN CARTILAGE 87
4.7.3.2 EXPRESSION O F SURFACTANT PROTEIN ANALYSED B Y RT-PCR 88
5. DISCUSSION 9 0
5.1 PROBLEMS AND LIMITATIONS 9 0
5.2 BOUNDARY LUBRICATION PROVIDED BY PL 92
5.3 COMPOSITION O F PL SPECIES 95
5.4 JOINT DISTRACTION TO STUDY MECHANICAL IMPACT ON PL 9 8
5.5 PHOSPHOLIPID SYNTHESIS AND RELEASE B Y FLS 99
5.6 SURFACTANT PROTEINS IN HUMAN SF 100
5.7 SUMMARY, CONCLUSIONS AND FUTURE PERSPECTIVES 101
6. APPENDIX 104
7. REFERENCES 111
8. DECLARATION 122
9. CURRICULUM VITAE 123
10. ACKNOWLEDGEMENTS 125
IV
|
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| author | Kosińska, Marta Krystyna 1982- |
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| building | Verbundindex |
| bvnumber | BV040719944 |
| ctrlnum | (OCoLC)914570019 (DE-599)DNB1028502877 |
| dewey-full | 616.7223 |
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| dewey-ones | 616 - Diseases |
| dewey-raw | 616.7223 |
| dewey-search | 616.7223 |
| dewey-sort | 3616.7223 |
| dewey-tens | 610 - Medicine and health |
| discipline | Agrar-/Forst-/Ernährungs-/Haushaltswissenschaft / Gartenbau Medizin |
| edition | 1. Aufl. |
| format | Thesis Book |
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| spelling | Kosińska, Marta Krystyna 1982- Verfasser (DE-588)1028606605 aut Boundary lubricants in osteoarthritic synovial fluid von Marta Krystyna Kosinska 1. Aufl. Gießen, Lahn VVB Laufersweiler Verlag 2012 XIII, 144 S. graph. Darst. txt rdacontent n rdamedia nc rdacarrier Zugl.: Gießen, Univ., Diss., 2012 osteoarthritis cabt arthritis cabt joint diseases cabt synovial fluid cabt lubricants cabt materials cabt techniques cabt Gelenkschmiere (DE-588)4156451-0 gnd rswk-swf Arthrose (DE-588)4143132-7 gnd rswk-swf Phospholipide (DE-588)4131432-3 gnd rswk-swf Krankheitsverlauf (DE-588)4208496-9 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Arthrose (DE-588)4143132-7 s Gelenkschmiere (DE-588)4156451-0 s Phospholipide (DE-588)4131432-3 s Krankheitsverlauf (DE-588)4208496-9 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025700194&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Kosińska, Marta Krystyna 1982- Boundary lubricants in osteoarthritic synovial fluid osteoarthritis cabt arthritis cabt joint diseases cabt synovial fluid cabt lubricants cabt materials cabt techniques cabt Gelenkschmiere (DE-588)4156451-0 gnd Arthrose (DE-588)4143132-7 gnd Phospholipide (DE-588)4131432-3 gnd Krankheitsverlauf (DE-588)4208496-9 gnd |
| subject_GND | (DE-588)4156451-0 (DE-588)4143132-7 (DE-588)4131432-3 (DE-588)4208496-9 (DE-588)4113937-9 |
| title | Boundary lubricants in osteoarthritic synovial fluid |
| title_auth | Boundary lubricants in osteoarthritic synovial fluid |
| title_exact_search | Boundary lubricants in osteoarthritic synovial fluid |
| title_full | Boundary lubricants in osteoarthritic synovial fluid von Marta Krystyna Kosinska |
| title_fullStr | Boundary lubricants in osteoarthritic synovial fluid von Marta Krystyna Kosinska |
| title_full_unstemmed | Boundary lubricants in osteoarthritic synovial fluid von Marta Krystyna Kosinska |
| title_short | Boundary lubricants in osteoarthritic synovial fluid |
| title_sort | boundary lubricants in osteoarthritic synovial fluid |
| topic | osteoarthritis cabt arthritis cabt joint diseases cabt synovial fluid cabt lubricants cabt materials cabt techniques cabt Gelenkschmiere (DE-588)4156451-0 gnd Arthrose (DE-588)4143132-7 gnd Phospholipide (DE-588)4131432-3 gnd Krankheitsverlauf (DE-588)4208496-9 gnd |
| topic_facet | osteoarthritis arthritis joint diseases synovial fluid lubricants materials techniques Gelenkschmiere Arthrose Phospholipide Krankheitsverlauf Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025700194&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT kosinskamartakrystyna boundarylubricantsinosteoarthriticsynovialfluid |