Tools and techniques in biomolecular science:
Saved in:
| Format: | Book |
|---|---|
| Language: | English |
| Published: |
Oxford
Oxford Univ. Press
2013
|
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | XV, 512 S., [4] Bl. Ill., graph. Darst. |
| ISBN: | 9780199695560 |
Staff View
MARC
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| 245 | 1 | 0 | |a Tools and techniques in biomolecular science |c ed. by Aysha Divan ... |
| 246 | 1 | 3 | |a Biomolecular science |
| 264 | 1 | |a Oxford |b Oxford Univ. Press |c 2013 | |
| 300 | |a XV, 512 S., [4] Bl. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
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| 999 | |a oai:aleph.bib-bvb.de:BVB01-025679700 | ||
Record in the Search Index
| _version_ | 1804150000853188608 |
|---|---|
| adam_text | Preface
vi
Contributors
ix
PART
1
Working with
DNA
and
RNA
Cene
cloning essentials
1.1
Introduction
4
1.2
Gene cloning applications
4
1.3
Gene cloning in the laboratory
5
1.4
Gene cloning processes
14
1.5
Further types of gene cloning
18
1.6
Chapter summary
21
Polymerase chain reaction
23
23
24
26
31
40
41
41
DNA
mutagenesis
44
3.1
Introduction
45
3.2
Rational or site-directed mutagenesis
45
3.3
Uracil-containing
DNA 47
3.4
QuikChange! mutagenesis
47
3.5
Multi-site mutagenesis
52
3.6
Cassette mutagenesis
54
3.7
PCR mutagenesis
56
3.8
Saturation mutagenesis
58
2.1
Introduction
2.2
How PCR works
2.3
The PCR protocol
2.4
PCR techniques and applications
2.5
Forensic
DNA
analysis
2.6
Future prospects
2.7
Chapter summary
XU
COMTNÌS
3.9
Random mutagenesis
60
ЗЛО
Chapter summary
61
DNA
sequencing
66
4.1
First-generation sequencing
67
4.2
Next-generation sequencing
77
4.3
Third-generation sequencing
85
4.4
Chapter summary
86
Measuring DNA-protein interactions
88
5.1
Introduction
89
5.2
Footprinting
91
5.3
Electrophoretic mobility shift assay (EMSA)
97
5.4
Chromatin immunoprecipitation
103
5.5
Chapter summary
112
RNA
interference technology
116
6.1
Regulation of gene expression: the
RNA
interference pathway
117
6.2
Applications of RNAi
119
6.3
Short interfering
RNA (siRNA)
122
6.4
Short hairpin
RNA (shRNA)
127
6.5
Targeted gene silencing: therapeutic possibilities
136
6.6
Chapter summary
139
PART
2
Working with protein
Recombinant
protein expression
145
7.1
Introduction
146
7.2
Choosing the right expression system
148
7.3
E. co/l-based expression systems
151
7.4
Yeast expression systems
157
7.5
Baculovirus-insect larval cell expression system
167
7.6
Chapter summary
171
Protein purification
175
8.1
Introduction
175
8.2
Devising a purification strategy
176
8.3
Cell lysis and disruption
181
8.4
Performing chromatography
183
8.5 Chromatographie
separation methods
184
хш
8.6
Measuring protein recovery
8.7
Chapter summary
192
194
Antibodies as research tools
9.1
Introduction
9.2
Antibody structure and function
9.3
Target detection and visualization
9.4
Do I need to make a new antibody?
9.5
Common experimental platforms for immunoassay
9.6
Chapter summary
196
196
197
200
202
209
215
И
Measuring protein-protein interactions: qualitative approaches
218
10.1
Introduction
10.2
Pull-down methods
10.3
Phage display for analysis of protein interactions
10.4
Yeast two-hybrid assay
10.5
Chapter summary
218
219
223
229
233
■ !■
Measuring protein-protein interactions: quantitative approaches
237
11.1
Introduction
11.2
Fluorescence spectroscopy
11.3
isothermal titration microcalorimetry
11.4
Surface plasmon resonance
11.5
Chapter summary
237
238
246
252
258
Structural analysis of proteins: X-ray crystallography, NMR, AFM,
and CD spectroscopy
12.1
X-ray crystallography
12.2
Nuclear magnetic resonance
12.3
Atomic force microscopy
12.4
Circular dkhroism spectroscopy
12.5
Chapter summary
261
262
269
276
286
291
Mass spectrometry
13.1
Introduction
13.2
Sample preparation and ¡onization
13.3
Mass analysis
13.4
Tandem mass spectrometry
13.5
Chapter summary
295
296
298
306
310
313
CONTENTS
Proteomic
analysis
318
14.1
Introduction
318
14.2
Gel-based proteomics
320
14.3
Protein identification by tandem mass spectrometry (MS/MS)
322
14.4
Post-translational modifications
324
14.5
Quantitative mass spectrometry
327
14.6
Data analysis
338
14.7
Chapter summary
339
PART
3
Working with cells and tissues
Ш
Cl
Culturing mammalian cells
343
15.1
Introduction
344
15.2
Culturing cells in vitro: essential principles
345
15.3
General applications
351
15.4
Human embryonic and adult stem cells: culture, isolation, and expansion
351
15.5
Stem cell culture protocols
355
15.6
Limitations of cell culturing methods
360
15.7
Ethical and storage issues
364
15.8
Chapter summary
364
368
КЯ
Flow cytometry
16.1
Flow cytometers: how they work
369
16.2
Data display and analysis
373
16.3
Flow cytometry application: immunophenotyping
378
16.4
Flow cytometry application: cell cycle analysis
382
16.5
Flow cytometry application: apoptosis and viability assessment
383
16.6
Cell sorting
387
16.7
Chapter summary
392
Bioimaging: light and electron microscopy
394
17.1
Fluorescence microscopy
395
17.2
Transmission electron microscopy
405
17.3
Correlative light electron microscopy
413
17.4
Chapter summary
415
Histopathology in biomolecular research
418
18.1
Introduction
419
18.2
Ethical approval and consent
420
18.3
Tissue organization and data storage
420
CONTENTS
XV
18.4
Obtaining tissue samples for research
18.5
Recognizing pathology
18.6
Tissue preparation
18.7
Microscopic analysis of tissue samples
18.8
Chapter summary
420
422
425
428
436
PART
4
Working with models in the biomolecular sciences
Mouse models in
bioscience
research
19.1
Introduction
19.2
Transgeme
mice
19.3
Gene targeted mice
19.4
Regulatable gene expression systems (Cre-lox models)
19.5
Regulatable gene expression systems (Tet-Off/Tet-On mice)
19.6
Chapter summary
441
441
443
449
455
459
462
M
Mathematical models in biomolecular sciences
20.1
Introduction
20.2
Difference equations
20.3
Cellular automata
20.4
Networks
20.5
Markov chains
20.6
Petri
nets
20.7
Ordinary differential equations (ODEs)
20.8
Chapter summary
466
466
469
472
474
476
477
478
480
Glossary
Index
484
504
|
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| indexdate | 2024-07-10T00:31:58Z |
| institution | BVB |
| isbn | 9780199695560 |
| language | English |
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| physical | XV, 512 S., [4] Bl. Ill., graph. Darst. |
| publishDate | 2013 |
| publishDateSearch | 2013 |
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| publisher | Oxford Univ. Press |
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| spelling | Tools and techniques in biomolecular science ed. by Aysha Divan ... Biomolecular science Oxford Oxford Univ. Press 2013 XV, 512 S., [4] Bl. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Molekularbiologische Methode (DE-588)7650063-9 gnd rswk-swf Molekularbiologische Methode (DE-588)7650063-9 s DE-604 Divan, Aysha Sonstige (DE-588)1208236512 oth Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025679700&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Tools and techniques in biomolecular science Molekularbiologische Methode (DE-588)7650063-9 gnd |
| subject_GND | (DE-588)7650063-9 |
| title | Tools and techniques in biomolecular science |
| title_alt | Biomolecular science |
| title_auth | Tools and techniques in biomolecular science |
| title_exact_search | Tools and techniques in biomolecular science |
| title_full | Tools and techniques in biomolecular science ed. by Aysha Divan ... |
| title_fullStr | Tools and techniques in biomolecular science ed. by Aysha Divan ... |
| title_full_unstemmed | Tools and techniques in biomolecular science ed. by Aysha Divan ... |
| title_short | Tools and techniques in biomolecular science |
| title_sort | tools and techniques in biomolecular science |
| topic | Molekularbiologische Methode (DE-588)7650063-9 gnd |
| topic_facet | Molekularbiologische Methode |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025679700&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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