Quantification of hypoxia by Hypoxia Inducible Factor-1alpha and its role in vascularization of the Arterio-Venous loop in a rat isolation chamber model:
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| Format: | Thesis Book |
| Language: | English |
| Published: |
2012
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| Subjects: | |
| Online Access: | Volltext http://d-nb.info/1026365406/34 https://nbn-resolving.org/urn:nbn:de:bvb:29-opus-35245 Inhaltsverzeichnis |
| Physical Description: | 70 S. Ill. |
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Record in the Search Index
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| adam_text | IMAGE 1
TABLE OF CONTENTS
ABSTRACT 1
ZUSAMMENSFASSUNG 3
1. INTRODUCTION: 5
1.1. TISSUE ENGINEERING 5
1.1.1. THE CHALLENGING: VASCULARIZATION 6
1.1.2. STRATEGIES FOR VASCULARIZATION: 7
1.2. AV LOOP MODEL 8
1.2.1. DEVELOPMENT OF AV LOOP 8
1.2.2. MATRIX IN AV LOOP MODEL: 9
1.2.3. INVESTIGATE METHODS FOR AV LOOPS: 11
1.3. HYPOXIA, HIF-1A AND VASCULOGENESIS 11
1.3.1. STRUCTURE 12
1.3.2. KINETIC OF INDUCTION AND DEGRADATION: 13
1.3.3. DEGRADATION BY HYDROXYLATION 13
1.3.4. ANGIOGENIC TARGET GENES AND MOLECULES 15
2. MATERIALS AND METHODS 17
2.1. RAT: 17
2.2. ISOLATION CHAMBER: 17
2.3. LIQUID REAGENT PREPARATION: 18
2.3.1. FIBRIN AND THROMBIN: 18
2.3.2. 4%PARAFORMALDEHYDE (PFA): 18
2.3.3. WASHING BUFFER 18
2.3.4. FAST RED CHROMOGEN 19
2.4. ANIMAL EXPERIMENTS 19
2.4.1. GROUP DESIGNS: 19
HTTP://D-NB.INFO/1026826136
IMAGE 2
2.4.2. PREOPERATIVE TREATMENT: 19
2.4.3. LOOP PREPARATION: 19
2.4.4. CHAMBER IMPLANTATION: 20
2.4.5. POSTOPERATIVE TREATMENT: 21
2.4.6. EXPLANTATION: 23
2.5. HISTOLOGY: 23
2.5.1. GENERAL PREPARATION: 23
2.5.2. IMMUNOHISTOCHEMISTRY (IHC) 25
2.5.3. GENERAL POST IHC PROCESSING 28
2.6. REGIONS OF INTEREST (ROI) DEFINITION IN THREE DIMENSIONS 29
2.6.1. REGIONS OF INTEREST (ROI) 29
2.6.2. COUNTING: 31
3. RESULTS: 32
3.1. HIF-1A POSITIVE AND GENERAL CELLS DISTRIBUTION 32
3.1.1. INTERACTION: DAY*DISTANCE: 33
3.2. HIF-1A AND PIMONIDAZOLE STAINING IN CONSECUTIVE SLIDES 36
3.3. ED1, HIF-1A CO LOCALIZATION 38
3.4. HIF-1A AND LECTIN 40
4. DISCUSSION: 43
4.1. THE PATTERN OF HYPOXIA WITHIN AV LOOP 44
4.1.1. THE HIF-1A POSITIVE RATE 44
4.1.2. CELL DISTRIBUTION 45
4.1.3. BS-I LECTIN VS LECTIN-HIF1A IHC 45
4.1.4. PIMONIDAZOLE VS HIF-1A 46
4.2. THE PATTERN OF VASCULARIZATION IN THE AV LOOP: 47
4.2.1. WHEN? 47
4.2.2. WHERE? 48
IMAGE 3
4.2.3. HOW? 48
4.2.4. WHY? 49
4.3. THE MODEL PREPARATION: WHAT IS CRUCIAL TO GAIN A PATENT AV LOOP? 50
4.4. CONCLUSION AND PERSPECTIVE 53
4.4.1. CONCLUSION 53
4.4.2. APPLICATION OF THE AV LOOP: 54
4.4.3. REGARDING THE ASSESSMENT FOR THE MODEL: WHERE TO PICK UP THE ROI
IN THE AV LOOP? 55
4.4.4. LIMITATION OF THE STUDY: 55
4.4.5. FURTHER PLAN: THE APPLICATION OF PHD INHIBITION 56
ACKNOWLEDGEMENT: 67
CURRICULUM VITAE 69
|
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| spelling | Yuan, Quan 1982- Verfasser (DE-588)1026797438 aut Quantification of hypoxia by Hypoxia Inducible Factor-1alpha and its role in vascularization of the Arterio-Venous loop in a rat isolation chamber model von Quan Yuan 2012 70 S. Ill. txt rdacontent n rdamedia nc rdacarrier Erlangen-Nürnberg, Univ., Diss., 2012 (DE-588)4113937-9 Hochschulschrift gnd-content Erscheint auch als Online-Ausgabe urn:nbn:de:bvb:29-opus-35245 https://open.fau.de/handle/openfau/2364 Verlag kostenfrei Volltext http://d-nb.info/1026365406/34 Langzeitarchivierung Nationalbibliothek https://nbn-resolving.org/urn:nbn:de:bvb:29-opus-35245 Resolving-System DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025484700&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Yuan, Quan 1982- Quantification of hypoxia by Hypoxia Inducible Factor-1alpha and its role in vascularization of the Arterio-Venous loop in a rat isolation chamber model |
| subject_GND | (DE-588)4113937-9 |
| title | Quantification of hypoxia by Hypoxia Inducible Factor-1alpha and its role in vascularization of the Arterio-Venous loop in a rat isolation chamber model |
| title_auth | Quantification of hypoxia by Hypoxia Inducible Factor-1alpha and its role in vascularization of the Arterio-Venous loop in a rat isolation chamber model |
| title_exact_search | Quantification of hypoxia by Hypoxia Inducible Factor-1alpha and its role in vascularization of the Arterio-Venous loop in a rat isolation chamber model |
| title_full | Quantification of hypoxia by Hypoxia Inducible Factor-1alpha and its role in vascularization of the Arterio-Venous loop in a rat isolation chamber model von Quan Yuan |
| title_fullStr | Quantification of hypoxia by Hypoxia Inducible Factor-1alpha and its role in vascularization of the Arterio-Venous loop in a rat isolation chamber model von Quan Yuan |
| title_full_unstemmed | Quantification of hypoxia by Hypoxia Inducible Factor-1alpha and its role in vascularization of the Arterio-Venous loop in a rat isolation chamber model von Quan Yuan |
| title_short | Quantification of hypoxia by Hypoxia Inducible Factor-1alpha and its role in vascularization of the Arterio-Venous loop in a rat isolation chamber model |
| title_sort | quantification of hypoxia by hypoxia inducible factor 1alpha and its role in vascularization of the arterio venous loop in a rat isolation chamber model |
| topic_facet | Hochschulschrift |
| url | https://open.fau.de/handle/openfau/2364 http://d-nb.info/1026365406/34 https://nbn-resolving.org/urn:nbn:de:bvb:29-opus-35245 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025484700&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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