Microbial Nitrogen cycling in soils at pioneer sites:
Saved in:
| Main Author: | |
|---|---|
| Format: | Thesis Book |
| Language: | English |
| Published: |
Göttingen
Sierke Verl.
2012
|
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | 161 S. graph. Darst. |
| ISBN: | 9783868444742 |
Staff View
MARC
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Record in the Search Index
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|---|---|
| adam_text | IMAGE 1
CONTENTS
SUMMARY 9
ZUSAMMENFASSUNG 13
1 GENERAL INTRODUCTION 17
1.1 ECOSYSTEM DEVELOPMENT 17
1.2 GLOBAL NITROGEN BUDGET 18
1.3 NITROGEN CYCLE 20
1.4 THERMODYNAMICAL CONSIDERATIONS 21
1.5 MICROORGANISMS IN THE NITROGEN CYCLE 22
1.6 METHODS TO STUDY MICROBIAL NITROGEN CYCLING 24
1.6.1 DNA BASED CHARACTERISATION 24
1.6.2 MRNA BASED CHARACTERISATION 27
1.6.3 PROTEIN BASED CHARACTERISATION 28
1.6.4 QUANTIFICATION ON NITROGEN TURNOVER LEVEL 29
1.7 BACKGROUND AND RESEARCH HYPOTHESES OF THIS DISSERTATION . . . . 30
1.7.1 RESEARCH FRAMEWORK AND STUDY SITES 30
1.7.2 HYPOTHESES 33
1.7.3 PUBLICATIONS 35
2 ABUNDANCES AND POTENTIAL ACTIVITIES OF NITROGEN CYCLING MICROBIAL
COMMUNITIES ALONG A CHRONOSEQUENCE OF A GLACIER FOREFIELD 37 2.1
ABSTRACT 38
2.2 INTRODUCTION 39
2.3 MATERIALS AND METHODS 40
2.3.1 SAMPLING SITE 40
2.3.2 NITROGEN DEPOSITION 41
2.3.3 SAMPLING PROCEDURE 42
2.3.4 SOIL CARBON AND NITROGEN CONTENT 42
2.3.5 ENZYME ASSAYS 44
2.3.6 DNA EXTRACTION AND QUANTIFICATION 45
2.3.7 REAL-TIME PCR 45
2.3.8 STATISTICAL ANALYSIS 46
5
HTTP://D-NB.INFO/1027964532
IMAGE 2
CONTENTS
2.4 RESULTS 46
2.4.1 SOIL PARAMETERS ALONG THE CHRONOSEQUENCE 46
2.4.2 POTENTIAL ENZYME ACTIVITIES 46
2.4.3 ABUNDANCE OF FUNCTIONAL GENES 48
2.4.4 CORRELATION OF ABUNDANCES AND ACTIVITIES 50
2.5 DISCUSSION 53
2.5.1 THE INITIAL STATE 53
2.5.2 PLANTS AS COMPETITORS FOR NUTRIENTS 54
2.5.3 PLANTS AS DRIVER FOR NITROGEN TURNOVER 55
2.5.4 LINKING POTENTIAL ENZYMATIC ACTIVITIES TO GENE ABUNDANCE 56 2.5.5
THE CHRONOSEQUENCE APPROACH 57
2.6 CONCLUSION 57
2.7 ACKNOWLEDGEMENTS 57
3 ABUNDANCE OF MICROBES INVOLVED IN NITROGEN TRANSFORMATION IN T H E
RHIZOSPHERE OF LEUCANTHEMOPSIS ALPINA (L.) HEYWOOD GROWN IN SOILS FROM
DIFFERENT SITES OF THE DAMMA GLACIER FOREFIELD 59
3.1 ABSTRACT 60
3.2 INTRODUCTION 61
3.3 MATERIALS AND METHODS 62
3.3.1 EXPERIMENTAL PROCEDURES 62
3.3.2 SOIL AMMONIUM AND NITRATE MEASUREMENT 64
3.3.3 CARBON AND NITROGEN CONTENT IN PLANTS 64
3.3.4 NUCLEIC ACID EXTRACTION 64
3.3.5 REAL-TIME PCR ASSAY 64
3.3.6 STATISTICAL ANALYSIS 65
3.4 RESULTS 67
3.4.1 NITROGEN AND CARBON CONTENT OF PLANT BIOMASS 67
3.4.2 AMMONIUM AND NITRATE CONCENTRATIONS IN BS AND RRC SAMPLES 67
3.4.3 ABUNDANCES OF FUNCTIONAL GENES OF THE NITROGEN CYCLE . . 68 3.5
DISCUSSION 71
3.6 ACKNOWLEDGEMENTS 73
4 SUCCESSION OF N CYCLING PROCESSES IN BIOLOGICAL SOIL CRUSTS ON A
CENTRAL EUROPEAN INLAND DUNE 75
4.1 ABSTRACT 76
4.2 INTRODUCTION 77
4.3 MATERIALS AND METHODS 79
4.3.1 STUDY SITE 79
4.3.2 HYDROLOGY 79
6
IMAGE 3
CONTENTS
4.3.3 BSCS AT THE STUDY SITE 79
4.3.4 SAMPLING PROCEDURE 81
4.3.5 SOIL CHEMICAL AND PHYSICAL ANALYSIS 81
4.3.6 POTENTIAL ENZYME ACTIVITIES 83
4.3.7 DNA EXTRACTION AND REAL-TIME PCR 83
4.4 RESULTS 84
4.4.1 SOIL PHYSICAL AND CHEMICAL CHARACTERISATION 84
4.4.2 DEVELOPMENT OF BSCS 86
4.4.3 POTENTIAL ENZYME ACTIVITY 86
4.4.4 GENE ABUNDANCE 89
4.5 DISCUSSION 89
4.5.1 IDENTIFICATION OF BSC DEVELOPMENT 89
4.5.2 DEVELOPMENT OF NITROGEN POOLS 91
4.5.3 HYDROLOGICAL EFFECTS ON NITROGEN POOLS 91
4.5.4 SUCCESSION OF NITROGEN CYCLING PROCESSES 92
4.5.5 CONCLUSIONS 94
4.6 ACKNOWLEDGEMENTS 94
5 SIMPLE ABSOLUTE QUANTIFICATION METHOD CORRECTING FOR QUANTITATIVE PCR
EFFICIENCY VARIATIONS FOR MICROBIAL COMMUNITY SAMPLES 97 5.1 ABSTRACT 98
5.2 INTRODUCTION 99
5.3 METHODS 101
5.3.1 BACTERIAL CULTURES 101
5.3.2 ENVIRONMENTAL SAMPLES 102
5.3.3 REAL-TIME PCR ASSAYS 103
5.3.4 DETERMINATION OF CT AND EFI 104
5.3.5 QUANTIFICATION METHODS 104
5.3.6 EFI OF BACTERIAL STRAINS AND ENVIRONMENTAL SAMPLES . . . . 106
5.3.7 ANALYSIS OF TEMPLATE MIXTURES FROM TWO SPECIES 106 5.3.8 ANALYSIS
OF ENVIRONMENTAL SAMPLES 106
5.4 RESULTS AND DISCUSSION 107
5.4.1 EFFICIENCY OF QPCR REACTIONS DEPENDS ON THE TEMPLATE . . 107 5.4.2
LIMITATION OF THE SC METHOD 109
5.4.3 HYPOTHETICAL COMPARISON OF SC AND OPC METHOD . . . . 109 5.4.4
EXPERIMENTAL COMPARISON OF THE SC AND OPC METHOD . . 114 5.4.5
QUANTIFICATION OF ENVIRONMENTAL SAMPLES BY SC AND OPC METHODS 117
5.4.6 LIMITATIONS OF THE OPC METHOD 117
5.4.7 CONCLUSION 119
7
IMAGE 4
CONTENTS
5.5 ACKNOWLEDGEMENTS 119
6 GENERAL DISCUSSION 121
6.1 EVIDENCE FOR MICROBIAL SUCCESSION IN SOIL 121
6.2 PRIMARY PRODUCERS KICK-START MICROBIAL SUCCESSION 122
6.3 DEVELOPMENT OF BIOGEOCHEMICAL CYCLES AT THE DAMMA GLACIER
CHRONOSEQUENCE 125
6.4 ACCOUNTING FOR HETEROGENEITY: MATCHING SAMPLING SETUP AND RE SEARCH
QUESTION 127
6.5 FUNCTIONAL GENE ANALYSIS: PROMISING TOOL 128
6.6 OUTLOOK 131
BIBLIOGRAPHY 133
ACKNOWLEDGEMENTS 159
CURRICULUM VITAE 161
8
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| spelling | Brankatschk, Robert 1982- Verfasser (DE-588)1028429223 aut Microbial Nitrogen cycling in soils at pioneer sites presented by Robert Brankatschk Göttingen Sierke Verl. 2012 161 S. graph. Darst. txt rdacontent n rdamedia nc rdacarrier Zugl.: Zürich, Techn. Hochsch., Diss., 2012 Stickstoffkreislauf (DE-588)4183276-0 gnd rswk-swf Urner Alpen (DE-588)4331785-6 gnd rswk-swf Lieberoser Heide (DE-588)7743926-0 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Urner Alpen (DE-588)4331785-6 g Lieberoser Heide (DE-588)7743926-0 g Stickstoffkreislauf (DE-588)4183276-0 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025418339&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Brankatschk, Robert 1982- Microbial Nitrogen cycling in soils at pioneer sites Stickstoffkreislauf (DE-588)4183276-0 gnd |
| subject_GND | (DE-588)4183276-0 (DE-588)4331785-6 (DE-588)7743926-0 (DE-588)4113937-9 |
| title | Microbial Nitrogen cycling in soils at pioneer sites |
| title_auth | Microbial Nitrogen cycling in soils at pioneer sites |
| title_exact_search | Microbial Nitrogen cycling in soils at pioneer sites |
| title_full | Microbial Nitrogen cycling in soils at pioneer sites presented by Robert Brankatschk |
| title_fullStr | Microbial Nitrogen cycling in soils at pioneer sites presented by Robert Brankatschk |
| title_full_unstemmed | Microbial Nitrogen cycling in soils at pioneer sites presented by Robert Brankatschk |
| title_short | Microbial Nitrogen cycling in soils at pioneer sites |
| title_sort | microbial nitrogen cycling in soils at pioneer sites |
| topic | Stickstoffkreislauf (DE-588)4183276-0 gnd |
| topic_facet | Stickstoffkreislauf Urner Alpen Lieberoser Heide Hochschulschrift |
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